Development of functional glyco-devices using fluorous interaction

• Project/Area Number: 19300168
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: The University of Tokyo
• Principal Investigator: HATANAKA Kenichi The University of Tokyo, 生産技術研究所, 教授 (70167584)
• Co-Investigator(Kenkyū-buntansha): KASUYA Mariacarmelita 東京大学, 生産技術研究所, 助教 (30334361) ; MIZUNO Mamoru (財)野口研究所, 研究部, 研究員 (40271506) ; TOJINO Mami (財)野口研究所, 研究部, 研究員 (70435551)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2007: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
• Keywords: 糖鎖 / 糖鎖生産 / 医用材料 / 細胞 / フルオラス / 糖鎖認識 / 糖鎖プライマー / レクチン / ウイルス / インフルエンザ / GM3 / 糖鎖の固定化 / プライマー / グリコシル化 / 臨界ミセル濃度 / 表面張力 / 細胞膜 / フルオラスタグ

Research Abstract

Glyco-compound having fluoroalkyl chain was synthesized and its carbohydrate chain was elongated using cells. The oligosaccharide having fluoroalkyl chain was immobilized on the fluoro-material and the immobilized material was tested for the absorption of influenza virus. It was found that the ethylene oxide oligomer was necessary as a spacer for recognition of influenza virus. It was confirmed that the immobilized oligosaccharide compound having oligo(ethylene oxide) spacer was specifically recognized by influenza virus.

Research Products

• [Journal Article] Evaluation of the Hydrophobicity of Perfluoroalkyl Chains in Amphiphilic Compounds That Are Incorporated into Cell Membrane (2011)
  • Author(s): M.C.Kasuya, S.Nakano, R.Katayama, K.Hatanaka
  • Journal Title: J.Fluorine Chem. Vol.132 Pages: 202-206
  • Peer Reviewed
• [Journal Article] Evaluation of the Hydrophobicity of Perfluoroalkyl Chains in Amphiphilic Compounds That Are Incorporated into Cell Membrane (2011)
  • Author(s): M.C.Z.Kasuya, S.Nakano, R.Katayama, K.Hatanaka
  • Journal Title: Journal of Fluorine Chemistry Volume: 132(掲載確定)
  • Peer Reviewed
• [Journal Article] Fluorous Tag for the Simultaneous Synthesis of Different Kinds of Glycolipids (2010)
  • Author(s): M.C.Z.Kasuya, M.Tojino, M.Mizuno, K.Hatanaka
  • Journal Title: J.Fluorine Chem. Vol.131 Pages: 655-659
  • Peer Reviewed
• [Journal Article] Fluorous Tag Methods for the Simultaneous Synthesis of Different Kinds of Glycolipids (2010)
  • Author(s): M.C.Z.Kasuya, M.Tojino, M.Mizuno, K.Hatanaka
  • Journal Title: Journal of Fluorine Chemistry Volume: 131 Pages: 655-659
  • Peer Reviewed
• [Journal Article] Effect of Fluorous Tag on Glycosylation of Saccharide Primers in Animal Cells (2009)
  • Author(s): M.C.Z.Kasuya, M.Tojino, S.Nakano, M.Mizuno, K.Hatanaka
  • Journal Title: Bull.Chem.Soc.Jpn. Vol.82 Pages: 1409-1415
  • NAID: 10027170349
  • Peer Reviewed
• [Journal Article] Effect of Fluorous Tags on Glycosylation of Saccharide Primers in Animal Cells (2009)
  • Author(s): M.C.Z.Kasuya, M.Tojino, S.Nakano, M.Mizuno, K.Hatanaka
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 82 Pages: 1409-1415
  • NAID: 10027170349
  • Peer Reviewed
• [Journal Article] Quantitative Analysis of EGFR Affinity to Immobilized Glycolipids by Surface Plasmon Resonance (2008)
  • Author(s): Y.Haga, S.-I.Hakomori, K.Hatanaka
  • Journal Title: Carbohydr.Res. Vol.343 Pages: 3034-3038
  • Peer Reviewed
• [Journal Article] Effect of Lipid Mimetics of GM3 and Lyso-GM3 Dimer on EGF Receptor Tyrosine Kinase and EGF-induced Signal Transduction (2008)
  • Author(s): Y.Haga, K.Hatanaka, S.-I.Hakomori
  • Journal Title: Biochim.Biophys.Acta Vol.1780 Pages: 393-404
  • Peer Reviewed
• [Journal Article] Purification of Gangliosides by Liquid-Liquid Partition Chromatography (2008)
  • Author(s): T.Kato, M.C.Kasuya, K.Hatanaka
  • Journal Title: J.Lipid Res. Vol.49 Pages: 2474-2478
  • Peer Reviewed
• [Journal Article] Inhibitory Effects of Glycopolymers Having Globotriose and/or Lactose on Cytotoxicity of Shiga-Toxin 1 (2008)
  • Author(s): A.Miyagawa, M.C.Kasuya, K.Hatanaka
  • Journal Title: Carbohydr.Polym. Vol.67 Pages: 260-264
  • Peer Reviewed
• [Journal Article] Purification of Gangliosides by liquid-liquid partition chromatography (2008)
  • Author(s): T.Kato, M.C.Kasuya, K.Hatanaka
  • Journal Title: J.Lipid Res. 49 Pages: 2474-2478
  • Peer Reviewed
• [Journal Article] Effect of lipid mimics of GM3 and lyso-GM3 dimer on EGF receptor tyrosine kinase and EGF-induced signal transduction (2008)
  • Author(s): Y.Haga, K.Hatanaka, S.Hakomori
  • Journal Title: Biochim.Biophys.Acta 1780 Pages: 393-404
  • Peer Reviewed
• [Journal Article] Quantitative analysis of EGFR affinity to immobilized glycolipids by surface plasmon resonance (2008)
  • Author(s): Y.Haga, K.Hatanaka, S.Hakomori
  • Journal Title: Carbohydr.Res. 343 Pages: 3034-3038
  • Peer Reviewed
• [Journal Article] Lyso-GM3, its dimer, and multimer : their synthesis and their effect on epidermal growth factorinduced receptor tyrosine kinase (2007)
  • Author(s): Y.Murozuka, N.Watanabe, K.Hatanaka, S.-I.Hakomori
  • Journal Title: Glycoconjugate J. Vol.24 Pages: 551-563
  • Peer Reviewed
• [Journal Article] Simple and Convenient Synthesis of a Fluorinated GM4 Analogue (2007)
  • Author(s): M.C.Kasuya, A.Ito, K.Hatanaka
  • Journal Title: J.Fluorine Chem. Vol.128 Pages: 562-565
  • Peer Reviewed
• [Journal Article] Simple and convenient synthesis of a fluorinated GM4 analogue (2007)
  • Author(s): M. C. Kasuya, A. Ito, K. Hatanaka
  • Journal Title: Journal of Fluorine Chemistry 128 Pages: 562-565
  • Peer Reviewed
• [Journal Article] Inhibitory effects of glycopolymers having globotriose and/or lactose on cytotoxicity of Shig (2007)
  • Author(s): A. Miyagawa, M. C. Kasuya, K. Hatan
  • Journal Title: Carbohydrate Polymers 67 Pages: 260-264
  • Peer Reviewed
• [Journal Article] Lyso-GM3, its dimer, and trimer : their synthesis, and their effect on epidermal growth factor (2007)
  • Author(s): Y. Murozuka, N. Watanabe, K. Hatan
  • Journal Title: Glycoconjugate Journal 24 Pages: 551-563
  • Peer Reviewed
• [Presentation] Incorporation of Fluorous Glycoside in Cell and Oligosaccharide Production (2009)
  • Author(s): K.Hatanaka
  • Organizer: International Symposium on Fluorous Technologies '09
  • Place of Presentation: Jackson Hole (USA)
  • Year and Date: 2009-08-25
• [Presentation] Cellular Uptake of Fluoroalkyl Glycoside and Saccharide-Chain Elongation (2008)
  • Author(s): S.Nakano
  • Organizer: 24th International Carbohydrate Symposium
  • Place of Presentation: Oslo (Norway)
  • Year and Date: 2008-07-28
• [Presentation] Cellular uptake of fluoroalkyl glycoside and saccharide-chain elongation (2008)
  • Author(s): S.Nakano, M.C.Kasuya, K.Hatanaka
  • Organizer: 24^<th> International Carbohydrate symposium
  • Place of Presentation: オスロ市(ノルウェー)
  • Year and Date: 2008-07-28
• [Presentation] Fluorinated Saccharide : Versatile Scaffords for Oligosaccharide Synthesis Using Cells (2007)
  • Author(s): M.C.Kasuya
  • Organizer: International Symposium on Fluorous Technologies '07
  • Place of Presentation: Yokohama (Japan)
  • Year and Date: 2007-07-30
• [Presentation] Fluorinated Saccharide : Versatile Scaffolds for Oligosaccharide Synthesis Using Cells (2007)
  • Author(s): M. C. Kasuya, K. Hatanaka
  • Organizer: The 2nd International Symposium on Fluorous Technologies
  • Place of Presentation: 横浜市
  • Year and Date: 2007-07-30
• [Book] 酵素利用技術大系(細胞内糖鎖合成装置による糖脂質合成) (2010)
  • Author(s): 畑中研一(分担執筆)
  • Total Pages: 993
  • Publisher: エヌ・ティー・エス
• [Book] 酵素利用技術大系(pp404-407:細胞内糖鎖合成装置による糖脂質合成) (2010)
  • Author(s): 畑中研一(分担執筆)
  • Total Pages: 993
  • Publisher: エヌ・ティー・エス
• [Remarks]
  • URL: http://www.chembio.t.u-tokyo.ac.jp/labs/hatanaka.html