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Development of next generation nano-biomaterials by cell surface engineering

Research Project

Project/Area Number 19300176
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionKeio University

Principal Investigator

FUJIMOTO Keiji  Keio University, 理工学部, 教授 (70229045)

Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2007: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords細胞 / 組織工学 / 接着性ペプチド / 人工細胞外マトリックス / 細胞表層改質 / 光固定化 / 細胞凝集体 / 細胞接着性ペプチド / ポリマーナノツール / 伸展性 / 増殖性 / 細胞間接着 / 細胞組織の再構築
Research Abstract

We tried an approach to construct 2- and 3-dimensional tissues using a ligand-immobilized polymer chain as an artificial extracellular matrix. This technique based on the cell-agglomerate engineering allowed the efficient cellular coating of artificial materials with complicated shapes for improvement of biocompatibility and facilitation of tissue reconstruction. Next, we proposed a novel method for enhancing ligand-receptor binding and modifying cell surface as artificial material. So, we prepared a multi-functional polymer (polymer nano-tool) consisted of ligand, photo-reactive group, biotin and disulfide. We found that cells modified with biotin-carrying nano-tools specifically adhered to an avidin-immobilized plate and the formation of cell agglomerates was mediated by adding avidin.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (42 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (12 results) (of which Peer Reviewed: 11 results) Presentation (18 results) Book (9 results) Remarks (3 results)

  • [Journal Article] In situ synthesis of polysaccharide nanoparticles via polyion complex of carboxymethyl cellulose and chitosan2011

    • Author(s)
      S.Kaihara, Y.Suzuki, K.Fujimoto
    • Journal Title

      Colloids and Surfaces B : Biointerfaces 8

      Pages: 343-348

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] In situ synthesis of polysaccharide nanoparticles via polyion complex of carboxy methyl cellulose and chitosan2011

    • Author(s)
      S.Kaihara, Y.Suzuki, K.Fujimoto
    • Journal Title

      Colloids and Surfaces B : Biointerfaces

      Volume: 85 Pages: 343-348

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Preparation of Sugar Polymer-Coated Nanocapsules by the Layer-by-Layer Deposition on the Liposome2009

    • Author(s)
      Y.Fukui, K.Fujimoto
    • Journal Title

      Langmuir 25

      Pages: 10020-10025

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of Sugar Polymer-Coated Nanocapsules by the Layer-by-Layer Deposition on the Liposome.2009

    • Author(s)
      Y. Fukui, K. Fujimoto
    • Journal Title

      Langmuir 25

      Pages: 10020-10025

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] The Preparation of Sugar Polymer-Coated Nanocapsules by the Layer-by-Layer Deposition on the Liposome2009

    • Author(s)
      Fukui, Y., Fujimoto, K.
    • Journal Title

      Langmuir 25

      Pages: 10020-10025

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simple and Natural Approaches to Create Particulate Materials2009

    • Author(s)
      Fujimoto, K.
    • Journal Title

      J.Jpn.Soc.Colour Mater. 82

      Pages: 219-223

    • NAID

      10024765944

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Formation and recovery of a cell sheet by a particle monolayer with the surface roughness2008

    • Author(s)
      M.Miura, K.Fujimoto
    • Journal Title

      Colloids and Surfaces B : Biointerfaces 66

      Pages: 125-133

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Formation and recovery of a cell sheet by a particle monolayer with the surface roughness.2008

    • Author(s)
      M. Mura, K. Fujimoto
    • Journal Title

      Colloids and Surfaces B: Biointerfaces 66

      Pages: 125-133

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Formation and recovery of a cell sheet by a particle monolayer withthe surface roughness2008

    • Author(s)
      M. Miura, K. Fujimoto
    • Journal Title

      Colloids and Surfaces B : Biointerfaces 66

      Pages: 125-133

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of bionanocapsules by the layer-bylayer deposition of polypeptides onto a liposome2007

    • Author(s)
      K.Fujimoto, Y.Fukui, T.Toyoda
    • Journal Title

      Macromolecules 40

      Pages: 5122-5128

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of bionanocapsules by the layer-bylayer deposition of polypeptides onto a liposome.2007

    • Author(s)
      K. Fujimoto, Y. Fukui, T. Toyoda
    • Journal Title

      Macromolecules 40

      Pages: 5122-5128

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Preparation of bionanocapsules by the layer-by-layer deposition of polypeptides onto a liposome2007

    • Author(s)
      Keiji Fujimoto
    • Journal Title

      Macromolecules 40

      Pages: 5122-5128

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Development of a Particle Nano-imprinting Technique by Core-shell Particles2010

    • Author(s)
      K.Fujimoto, H.Watanabe
    • Organizer
      2010 MRS Fall Meeting
    • Place of Presentation
      Sheraton Boston Hotel (MA, USA)
    • Year and Date
      2010-12-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光反応性バイオマテリアルを用いた細胞表面改質と細胞組織体の構築2010

    • Author(s)
      齋藤真, 貝原祥子, 藤本啓二
    • Organizer
      第59回高分子討論大会, 高分子学会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 反応性バイオナノツールを用いた細胞表層改質および活性制御2010

    • Author(s)
      貝原祥子, 藤本啓二
    • Organizer
      第37回医用高分子シンポジウム, 高分子学会
    • Place of Presentation
      東大先端研(東京)
    • Year and Date
      2010-07-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Simple Designs of Nanobio-Materials by Learning from Bio-Interfaces2009

    • Author(s)
      K.Fujimoto
    • Organizer
      NIMS Week 2009
    • Place of Presentation
      Tsukuba
    • Year and Date
      2009-07-22
    • Related Report
      2010 Final Research Report
  • [Presentation] Simple Designs of Nanobio-Materials by Learning from Bio-Interfaces2009

    • Author(s)
      藤本啓二
    • Organizer
      NIMS WEEK 2009
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2009 Annual Research Report
  • [Presentation] 光反応性ポリマーナノツールを用いた細胞表層改質および活性制御2009

    • Author(s)
      住元彩香、藤本啓二
    • Organizer
      第31回日本バイオマテリアル学会大会
    • Place of Presentation
      京都府民総合交流プラザ
    • Related Report
      2009 Annual Research Report
  • [Presentation] 細胞凝集体が拓く生体組織構築に向けたアプローチ2008

    • Author(s)
      中畝明菜, 藤本啓二
    • Organizer
      第57回高分子学会討論会
    • Place of Presentation
      大阪市立大学、大阪
    • Year and Date
      2008-09-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] マルチリガンド型ポリマーによる細胞構造体のかたちづくり2008

    • Author(s)
      中畝明菜, 藤本啓二
    • Organizer
      第37回医用高分子シンポジウム
    • Place of Presentation
      東京医科歯科大学、東京
    • Year and Date
      2008-07-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] マルチリガンド型ポリマーを用いた3次元細胞構造体の構築と制御2008

    • Author(s)
      中畝明菜, 藤本啓二
    • Organizer
      第57回高分子学会年次大会
    • Place of Presentation
      横浜パシフィコ、横浜
    • Year and Date
      2008-05-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] Effect of nano-scale roughness on detachment of a cell sheet from a particle monolayer2008

    • Author(s)
      M.Miura, K.Fujimoto
    • Organizer
      The 8th World Biomaterials Congress
    • Place of Presentation
      Amsterdam, The Netherlands
    • Year and Date
      2008-05-28
    • Related Report
      2010 Final Research Report
  • [Presentation] Effect of nano-scale roughness on detachment of a cell sheet from a particle monolayer.2008

    • Author(s)
      M.Miura, K.Fujimoto
    • Organizer
      The 8th World Biomaterials Congress
    • Place of Presentation
      Amsterdam, The Netherlands
    • Year and Date
      2008-05-28
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Effect of nano-scale roughness on detachment of a cell sheet from a particlemonolayer2008

    • Author(s)
      M. Miura, K. Fujimoto
    • Organizer
      The 8^<th> World Biomaterials Congress
    • Place of Presentation
      Amsterdam, The Netherlands
    • Year and Date
      2008-05-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] ポリマー鎖を用いた細胞構造体の作製および生体組織工学への応用2007

    • Author(s)
      藤本 啓二
    • Organizer
      高分子学会討論大会
    • Place of Presentation
      名古屋工業大学
    • Year and Date
      2007-09-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] ナノスケール表面構造が創り出す細胞のかたち-細胞を単層で剥離する-2007

    • Author(s)
      藤本 啓二
    • Organizer
      高分子学会年次大会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2007-05-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] 力学的強度の異なるハイドロゲル粒子膜の作製と組織工学材料としての応用2007

    • Author(s)
      藤本 啓二
    • Organizer
      高分子学会年次大会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2007-05-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] ポリマー微粒子を用いた量子ドットの配列制御と蛍光特性制御2007

    • Author(s)
      藤本 啓二
    • Organizer
      高分子学会年次大会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2007-05-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] リボソームをテンプレートとする多糖被覆ナノバイオカプセルの作製2007

    • Author(s)
      藤本 啓二
    • Organizer
      高分子学会年次大会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2007-05-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] アットリアクターを用いたバイオミネラリゼーションによる生体適合性表面の構築2007

    • Author(s)
      藤本 啓二
    • Organizer
      高分子学会年次大会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2007-05-29
    • Related Report
      2007 Annual Research Report
  • [Book] バイオマテリアルの基礎2010

    • Author(s)
      藤本啓二,貝原祥子
    • Publisher
      東京医学館
    • Related Report
      2010 Final Research Report
  • [Book] バイオマテリアルの基礎,再生医療に必要なバイオマテリアル2010

    • Author(s)
      藤本啓二, 貝原祥子
    • Total Pages
      259
    • Publisher
      東京医学館
    • Related Report
      2010 Annual Research Report
  • [Book] 生体に学ぶ微粒子材料の創製2008

    • Author(s)
      藤本啓二
    • Publisher
      化学工業社
    • Related Report
      2010 Final Research Report 2009 Self-evaluation Report
  • [Book] 次世代医療のための高分子材料工学, 第2章細胞機能制御界面創製技術, 1. 微粒子集積表面での細胞挙動の観察と制御の試み2008

    • Author(s)
      藤本啓二
    • Total Pages
      286
    • Publisher
      シーエムシー出版
    • Related Report
      2008 Annual Research Report
  • [Book] 環境調和型新材料シリーズ, 生体材料, 3.7高分子微粒子, 3.7.1生体と高分子の接点を探る2008

    • Author(s)
      藤本啓二
    • Total Pages
      295
    • Publisher
      日刊工業新聞社
    • Related Report
      2008 Annual Research Report
  • [Book] 細胞と遊ぶ2008

    • Author(s)
      藤本啓二
    • Total Pages
      92
    • Publisher
      バイオマテリアル-生体材料-
    • Related Report
      2008 Annual Research Report
  • [Book] 生体に学ぶ微粒子材料の創製2008

    • Author(s)
      藤本 啓二
    • Publisher
      化学工業社
    • Related Report
      2007 Annual Research Report
  • [Book] ポリマーと複合化したナノ蛍光体の作製と応用2007

    • Author(s)
      藤本 啓二
    • Publisher
      シーエムシー
    • Related Report
      2007 Annual Research Report
  • [Book] 生体非吸収性高分子からなる微粒子の作製と特徴2007

    • Author(s)
      藤本 啓二
    • Publisher
      メディカルドゥ
    • Related Report
      2007 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.applc.keio.ac.jp/~fujimoto/lab.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.applc.keio.ac.jp/~fujimoto/lab.html

    • Related Report
      2009 Self-evaluation Report
  • [Remarks]

    • URL

      http://www.applc.keio.ac.jp/~fujimoto/lab.html

    • Related Report
      2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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