| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2007: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Research Abstract |
We tried an approach to construct 2- and 3-dimensional tissues using a ligand-immobilized polymer chain as an artificial extracellular matrix. This technique based on the cell-agglomerate engineering allowed the efficient cellular coating of artificial materials with complicated shapes for improvement of biocompatibility and facilitation of tissue reconstruction. Next, we proposed a novel method for enhancing ligand-receptor binding and modifying cell surface as artificial material. So, we prepared a multi-functional polymer (polymer nano-tool) consisted of ligand, photo-reactive group, biotin and disulfide. We found that cells modified with biotin-carrying nano-tools specifically adhered to an avidin-immobilized plate and the formation of cell agglomerates was mediated by adding avidin.
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