Study on photosynthetic water oxidation chemistry in photosystem II

• Project/Area Number: 19370061
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Ibaraki University
• Principal Investigator: ONO Takaaki Ibaraki University, 工学部, 教授 (10175268)
• Co-Investigator(Kenkyū-buntansha): MINO Hiroyuki 名古屋大学, 理学研究科, 准教授 (70300902) ; MIZUSAWA Naoki 東京大学, 総合文化研究科, 助教 (80342856)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2007: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 光生物 / 光合成 / 光化学系II / Mnクラスター / 酸素発生系 / FTIR / ESR / 水分解反応 / 生物物理学 / 光合成光 / 化学系II

Research Abstract

Fourier transform infrared(FTIR) spectra for reaction intermediates in photosynthetic water oxidation were measure at low frequency in ^<17>O and ^<18>O raveled water in PSII core samples were prepared from thermophilic cyanobacterium Thermocynechococcus elongatus and the infrared bands responsible for direct interaction between Mn and substrate water were assigned in every reaction intermediate states of the water oxidation. FTIR spectra were also measured in site-directed mutants of Synechocystis sp.PCC6803. Based on the results, roles of D1 C-terminal alanine as a ligand were evaluated.

Research Products

• [Journal Article] Temperature-sensitive reaction of a pho-tosensor protein YcgF : Possibility of a role of temperature sensor. (2010)
  • Author(s): Nakasone Y., Ono T., Ishi A., Masuda S., Terazima M.
  • Journal Title: Biochemistry 49 Pages: 2288-2296
  • Peer Reviewed
• [Journal Article] Temperature-sensitive reaction of a photosensor protein YcgF : Possibility of a role of temperature sensor (2010)
  • Author(s): Nakasone, Y., Ono, T., Ishi A., Masuda, S., Terazima M.
  • Journal Title: Biochemistry 49 Pages: 2288-2296
  • Peer Reviewed
• [Journal Article] Tempearture sensitive reaction of a photosensor protein YcgF : Possiblty of a role of temperature sensor (2010)
  • Author(s): Nakasone Yusuke
  • Journal Title: Biochemistry 49 Pages: 2288-2296
  • Peer Reviewed
• [Journal Article] 熱発光・遅延発光 (2009)
  • Author(s): 小野高明
  • Journal Title: 低温科学 67 Pages: 453-463
  • Peer Reviewed
• [Journal Article] Involvement of digalactosyldiacylglycerol in cellular thermotolerance of Synechocystis sp. PCC 6803. (2009)
  • Author(s): Mizusawa N., Sakata S., Sakurai I., Sato N., Wada H.
  • Journal Title: Arch. Microbiol. 191 Pages: 595-601
  • Peer Reviewed
• [Journal Article] Crucial Role in light signal transduction for the conserved Met93 of the BLUF protein PixD/Slr1694. (2008)
  • Author(s): Masuda S., Hasegawa K., Ohta H., Ono T.A.
  • Journal Title: Plant Cell Physiol. 49 Pages: 1600-1606
  • NAID: 10027334810
  • Peer Reviewed
• [Journal Article] Crucial Role in light signal transduction for the conserved Met93 of the BLUF protein PixD/Slr1694 (2008)
  • Author(s): Masuda, S., Hasegawa, K., Ohta, H., Ono, T. A.
  • Journal Title: Plant Cell Physiol. 49 Pages: 1600-1606
  • NAID: 10027334810
  • Peer Reviewed
• [Journal Article] Crucial Role in Light Signaling Transition for the Conserved Met93 of the BLUF Protein PixD/Slr1694 (2008)
  • Author(s): Masuda Shinji
  • Journal Title: Plant Cell Physiology 49 Pages: 1600-1606
  • Peer Reviewed
• [Journal Article] Transient dimerization and confor-mational change of a BLUF Protein : YcgF. (2007)
  • Author(s): Nakasone Yusuke, Ono Taka-aki, Ishii Asako, Shinji Masuda, Terazima Masahide
  • Journal Title: J.Am.Chem.Soc. 129 Pages: 7028-7035
  • Peer Reviewed
• [Journal Article] Transient dimerization and conformational change of a BLUF Protein : YcgF (2007)
  • Author(s): Nakasone Yusuke, Ono Taka-aki, Ishii Asako, Shinji Masuda, Terazima Masahide
  • Journal Title: J.Am.Chem.Soc. 129 Pages: 7028-7035
  • Peer Reviewed
• [Journal Article] Transient Dimerization and Conformational Change of a BLUF Protein: YcgF (2007)
  • Author(s): Nakasone Yusuke
  • Journal Title: Journal Am. Chem. Soc. 129 Pages: 7028-7035
  • Peer Reviewed
• [Presentation] 青色光BLUF受容体 (2009)
  • Author(s): 小野高明
  • Organizer: 第2回生体分子科学コロキウム
  • Place of Presentation: 茨城量子ビームサイエンスセンター、那珂
  • Year and Date: 2009-06-24
• [Presentation] フーリエ変換赤外分光法による光合成酸素発生研究 (2009)
  • Author(s): 小野高明
  • Organizer: 第6回茨城大学遺伝子実験施設公開シンポジウム
  • Place of Presentation: 茨城大学、阿見
  • Year and Date: 2009-03-10
• [Presentation] FTIR studies on blue-light receptor using FAD (BLUF) (2008)
  • Author(s): 小野高明
  • Organizer: Joint Symposium of 5th Japan-China Crossover Science Symposium
  • Place of Presentation: 水戸・茨城大学
  • Year and Date: 2008-02-28
• [Presentation] FTIR studies on blue-light receptor using FAD (BLUF) Joint Symposium of 5th Japan-China (2008)
  • Author(s): 小野高明
  • Organizer: Crossover Science Symposium
  • Place of Presentation: 水戸・茨城大学
  • Year and Date: 2008-02-28
• [Presentation] 青色光センサーBLUF蛋白質YcgFの温度依存的会合とその光反応 (2008)
  • Author(s): 中曽根祐介
  • Organizer: 2008光化学討論会
  • Place of Presentation: 大阪府立大学中百舌鳥キャンパス
• [Presentation] BLUF蛋白質YcgFの温度依存的反応分子機構-温度センサーとしての可能性- (2008)
  • Author(s): 中曽根祐介
  • Organizer: 第35回生体分子科学討論会
  • Place of Presentation: 兵庫県先端科学技術支援センター
• [Presentation] 青色光センサーBLUF蛋白質YcgFの温度依存的会合とその光反応 (2008)
  • Author(s): 中曽根祐介
  • Organizer: 光化学討論会
  • Place of Presentation: 大阪府立大学 中百舌鳥キャンパス
• [Presentation] BLUF蛋白質YcgFの温度依存的反応分子機構 -温度センサーとしての可能性- (2008)
  • Author(s): 中曽根祐介
  • Organizer: 第35回 生体分子科学討論会
  • Place of Presentation: 兵庫県先端科学技術支援センター
• [Presentation] FTIR studies on blue-light receptor using FAD (BLUF)
  • Author(s): 小野高明
• [Remarks] ホームページ等
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• [Remarks] 茨城大学研究者情報総覧
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• [Remarks] 茨城大学研究者情報総覧
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