Production of a new low energy photosynthesis with chlorophylld : Understanding of principal of photosynthesis

• Project/Area Number: 19370064
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Nagoya University
• Principal Investigator: ITOH Shigeru Nagoya University, 大学院・理学研究科, 教授 (40108634)
• Co-Investigator(Kenkyū-buntansha): MINO Hiroyuki 名古屋大学, 大学院・理学研究科, 准教授 (70300902) ; UDUMAKI Tatsuya 名古屋大学, 大学院・理学研究科, 研究員 (60402418) ; ISHIURA Masahiro 名古屋大学, 遺伝子実験施設, 教授 (20132730)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2008: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2007: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
• Keywords: 光合成 / 酸素発生 / クロロフィル / 電子移動 / 光生物 / シアノバクテリア / 植物生理 / レーザ分光 / 光化学系 / 電子伝達系 / 遺伝子操作 / 色素機能 / 生体エネルギー

Research Abstract

クロロフィルdをもつ新発見の光合成生物Acaryochloris marina の光合成系の反応中心複合体及び光捕集系タンパク質の機能と構造その遺伝子をを検討した。クロロフィルd 合成系の原因遺伝子は未特定におわったが、クロロフィルdを使う光合成系の特性を明らかにした。多様な生物の光合成系の比較研究を行い、光合成光反応系の構築原理を検討した。新規の光センサータンパク質についても研究した。

Research Products

• [Journal Article] Heat stress causes inhibition of the de nove synthesis of antenna proteins and photobleaching in cultured Symbiodinium. (2008)
  • Author(s): Takahashi, S., Whitney, S. Itoh, S.. Maruyama, T., Badger, M
  • Journal Title: Proc. Natl. Acad. Science, USA 105 Pages: 4203-4208
  • Peer Reviewed
• [Journal Article] Parallel electron donation pathways to cytochrome cz in the type I homodimeric photosynthetic reaction center complex of Chlorobium tepidum (2008)
  • Author(s): Tsukatani, Y., Azai, C., Kondo, T., Itoh, S., Oh-oka, H
  • Journal Title: Biochim. Biophys. Acta 1777 Pages: 1211-1217
  • Peer Reviewed
• [Journal Article] A novel photoactive GAF domain of cyanobacteriochrome AnPixJ that shows reversible green/red photoconversion (2008)
  • Author(s): Narikawa, R., Fukushima, Y., Ishizuka, T., Itoh, S.., Ikeuchi, M
  • Journal Title: J. Mol. Biol 380 Pages: 844-855
  • Peer Reviewed
• [Journal Article] Photoreactions of Tyr8- and Gln50-mutated BLUF domains of the PixD protein of Thermosynechococcus elongatus BP-1 : photoconversion at low temperature without Tyr8 (2008)
  • Author(s): Fukushima Y, Murai Y, Okajima K, Ikeuchi M, Itoh, S
  • Journal Title: Biochemistry 47 Pages: 660-9
  • Peer Reviewed
• [Journal Article] An electron spin-polarized signal of the P800+A1(Q)- state in the homodimeric reaction center core complex of Heliobacterium modesticaldum. Miyamoto (2008)
  • Author(s): R, Mino, H, Kondo, T, Itoh, S., Oh-Oka, H
  • Journal Title: Biochemistry 47 Pages: 4386-93
  • Peer Reviewed
• [Journal Article] Temperature-dependent energy gap of the primary charge separation in photosystem I : study of delayed fluorescence at 77-268 (2008)
  • Author(s): K. Shibata, Y., Akai, S., Kasahara, T., Ikegami, I., Itoh,S
  • Journal Title: J Phys Chem B 112 Pages: 6695-6702
  • Peer Reviewed
• [Journal Article] Role of phosphatidylglycerol in the function and assembly of Photosystem II in a cdsA-inactivated PAL mutant strain of Synechocystis sp. PCC6803 that lacks phycobilisomes (2008)
  • Author(s): Laczko-Dobos H, Ughy B, Toth SZ., Komenda J.,, Zsiros O, Domonkos I, Parducz A, Bogos B, Komura M, Itoh, S. Gombos Z
  • Journal Title: Biochim Biophys Acta 1777 Pages: 1184-94
  • Peer Reviewed
• [Journal Article] Photosystem I complexes associated with fucoxanthin-chlorophyll-binding proteins from a marine centric diatom, Chaetoceros gracilis (2008)
  • Author(s): Ikeda Y, Komura M, Watanabe M, Minami C, Koike H, Itoh, S. Kashino, Y., Satoh, K
  • Journal Title: Biochim Biophys Acta 1777 Pages: 351-61
  • Peer Reviewed
• [Journal Article] Covalently linked zinc chlorophyll dimers as a model of a chlorophyllous pai in photosynthetic reaction centers (2008)
  • Author(s): Tamiaki,H., Kazuhiro Fukai, H., Shimazu, H., Nishide, K., Shibata, Y., Itoh, S. and Kunieda M
  • Journal Title: Photochem. Photobiol. Sci. 7 Pages: 1231-1237
  • Peer Reviewed
• [Journal Article] Photosystem I complexes associated with fucoxanthin-chlorophyll-binding proteins from a marine centric diatom, Chaetoceros gracilis. (2008)
  • Author(s): Ikeda Y, Komura M, Watanabe M, Minami C, Koike H, Itoh, S. Kashino, Y., Satoh, K.
  • Journal Title: Biochim Biophys Acta 1777 Pages: 351-361
  • Peer Reviewed
• [Journal Article] An electron spin-polarized signal of the P800+Al(Q)- state in the homodimeric reaction center core complex of Heliobacterium modesticaldum. (2008)
  • Author(s): Miyamoto, R, Mino, H, Kondo, T, Itoh, S. ^*, Oh-Oka, H.
  • Journal Title: Biochemistry 47 Pages: 4386-4393
  • Peer Reviewed
• [Journal Article] Heat stress causes inhibition of the de nove synthesis of antenna proteins and photobleaching in cultured Symbiodinium. (2008)
  • Author(s): Takahashi, S., Whitney, S. Itoh, S. Maruyama, T., Badger, M.
  • Journal Title: Proc. Natl. Acad. Science, USA 105 Pages: 4203-4208
  • Peer Reviewed
• [Journal Article] Photoreactions of Tyr8-and Gln50-mutated BLUF domains of the PixD Protein of Thermosynechococcus elongatus BP-1: Photoconversion at low temperature without Tyr-8 (2008)
  • Author(s): Fukushima, Y., Okajima, K., Shibata, Y., Ikeuchi, M. and Itoh, S.
  • Journal Title: Biochemistry 47 Pages: 660-669
  • Peer Reviewed
• [Journal Article] Heat stress causes inhibition of the de nove synthesis of antenna proteins and photobleaching in cuItured Symbiodinium (2008)
  • Author(s): Takahashi, S., Whitney, S. Itoh, S. Maruyama, T., Badger, M.
  • Journal Title: Proc. Natl. Acad. Science, USA 105 Pages: 4203-4208
  • Peer Reviewed
• [Journal Article] Function of chlorophyll d in reaction centers of photosystems I and II of the oxygenic photosynthesis of Acaryochloris marina (2007)
  • Author(s): Itoh, S., Mino, H., Itoh, K., Shigenaga, T., Uzumaki, T. and Iwaki, M
  • Journal Title: Biochemistry 46 Pages: 12473-12481
  • Peer Reviewed
• [Journal Article] Energy and electron transfer in the photosynthetic reaction center complex of Acidiphilium rubrum containing Zn-bacteriochlorophyll a studied by femtosecond up-conversion spectroscopy (2007)
  • Author(s): Tomi, T., Shibata, Y., Ikeda, Y., Taniguchi, S., Chosrowjan, H., Mataga, N., Shimada, K., Itoh, S
  • Journal Title: Biochim. Biophys. Acta 1767 Pages: 22-30
  • Peer Reviewed
• [Journal Article] Protons bound to the Mn cluster in photosystem II oxygen evolving complex detected by proton matrix ENDOR (2007)
  • Author(s): Yamada, H., Mino, H. and Itoh, S
  • Journal Title: Yamada, H., Mino, H. and Itoh, S 1767 Pages: 197-203
  • Peer Reviewed
• [Journal Article] Polarized fluorescence of aggregated bacteriochlorophyll c and baseplate bacteriochlorophyll a in single chlorosomes isolated from Chloroflexus aurantiacus (2007)
  • Author(s): Shibata, Y., Saga, Y., Tamiaki, H. and Itoh, S
  • Journal Title: Biochemistry 46 Pages: 7062-7068
  • Peer Reviewed
• [Journal Article] Excited-state dynamics of normal and doubly N-confused type hexaphyrin derivatives studied by time-resolved fluorescence measurements (2007)
  • Author(s): Ryu, J-H., Ito, F., Nagamura, T., Nakamura, K., Furuta, H., Shibata, Y. and Itoh, S
  • Journal Title: Chem. Phys. Lett. 443 Pages: 274-279
  • Peer Reviewed
• [Journal Article] Two intermediate states I and J trapped at low temperature in the photocycles of two BLUF domain proteins of cyanobacteria Synechocystis sp. PCC6803 and Thermosynechococcus elongatus BP-1 (2007)
  • Author(s): Fukushima, Y., Okajima, K., Ikeuchi, M., Itoh, S
  • Journal Title: Photochem. Photobiol 83 Pages: 112-121
  • Peer Reviewed
• [Journal Article] Function of chlorophyll d in reaction centers of photosystems I and II of the oxygenic photosynthesis of Acaryochloris marina (2007)
  • Author(s): Itoh, S., Mino, H., Itoh, K., Shigenaga, T., Uzumaki, T. and Iwaki, M.
  • Journal Title: Biochemistry 46 Pages: 12473-12481
  • Peer Reviewed
• [Presentation] 光センサータンパク質AnPixJ のシリカメソ多孔体内への導入 (2009)
  • Author(s): 富田祐介、青木俊、野地智康、上滝千尋、宇津巻竜也、神哲郎、成川礼、池内昌彦、梶野勉、伊藤繁
  • Organizer: 生物物理学会中部支部講演会
  • Place of Presentation: 名古屋大学
• [Presentation] シリカメソ多孔体への光合成膜タンパク質光化学系I の導入とその機能の評価 (2009)
  • Author(s): 上滝千尋、石坂壮二、野地智康、梶野勉、福嶋喜章、関藤武士、伊藤繁
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] 光センサータンパク質AnPixJのシリカメソ多孔体内への導入 (2009)
  • Author(s): 富田祐介、青木俊、野地智康、上滝千尋、宇津巻竜也、神哲郎、成川礼、池内昌彦、梶野勉、伊藤繁
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] 極低温フェムト-ピコ秒時間分解蛍光測定による乾燥地衣類光化学系II の高速励起エネルギー消光過程の解析 (2009)
  • Author(s): 小村理行、三宅博之、山岸篤史、柴田穣、岩崎郁子、山本好和、伊藤繁
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] 珪藻の光化学系Iアンテナーコア超分子複合体上での超高速励起エネルギー移動 (2009)
  • Author(s): 山岸篤史、池田洋平、小池裕幸、佐藤和彦、小村理行、柴田穣、伊藤繁
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] 極低温での光化学系I 反応中心の超高速エネルギー捕集過程の解析 (2009)
  • Author(s): 柴田 穣、山岸 篤史、小村 理行、伊藤繁
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] PSI・II反応中心のシリカナノポア内への導入とクロロフィルやキノンの置換 (2008)
  • Author(s): 伊藤繁
  • Organizer: 第46回日本生物部理学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-05
• [Presentation] Fluorescence Studies of single cells of Cyanobacteria and Lichen (2008)
  • Author(s): S. Itoh, K Sugiura, K Satoh, and M. Komura
  • Organizer: 4th Asia Oceania Conference of photobiology
  • Place of Presentation: Baranasi, India
• [Presentation] Picosecond and confocal fluorescence studies of chlorophylls in cyanobacteria, lichen and higher plant at 4K and room temperature (2008)
  • Author(s): S. Itoh, K. Sugiura, S. Keisuke, Y. Shibata, and M. Komura
  • Organizer: International Conference "Photosynthesis in the Global Perpectve"
  • Place of Presentation: Devi Ahilya Univ. Indore, Madhya Pradesh, India
• [Presentation] The S-state dependence of the location and affinity of the protons bound to the manganese cluster in photosystem II (2008)
  • Author(s): H. Mino
  • Organizer: International Conference "Photosynthesis in the Global Perpectve"
  • Place of Presentation: Devi Ahilya Univ. Indore, Madhya Pradesh, India
• [Presentation] Pulsed EPR Study of the Light-induced Radicals in a Blue Light Sensor Protein TePixD (2008)
  • Author(s): H. Mino, H. Nagai, Y. Fukushima, K. Okajima, M. Ikeuchi and S. Itoh
  • Organizer: Asia Pacific EPR Society-EPR Symposium 2008
  • Place of Presentation: Cairns Convention Centre, Cairns, Australia
• [Presentation] Chlorophyll-Proteins Inside Silica Mesoporous Material ; Function of Bacterial Light Harvesting-II Complex and Photosystem I Reaction Center Complex (2008)
  • Author(s): C. Kamidaki, I. Oda, S. Ishizaka, T. Noji, D. Fujita, T. Kajino, Y. Fukushima, T. Sekitoh, and S. Itoh
  • Organizer: IUMRS-ICA2008
  • Place of Presentation: Nagoya congress center, Nagoya
• [Presentation] 極低温時間分解蛍光測定による中心目珪藻Chaetoceros gracillis の光化学系I 超分子複合体内のエネルギー移動の解析 (2008)
  • Author(s): 山岸篤史、池田洋平、小池裕幸、佐藤和彦、小村理行、柴田穣、伊藤繁
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 対称型光合成反応中心タンパク質複合体内で機能する電子伝達体の配位構造 (2008)
  • Author(s): 近藤徹, 松岡昌弘, 浅井智広, 吉岡哲記, 三野広幸, 大岡宏造, 伊藤繁
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] ジビニルクロロフィルを持つシアノバクテリア変異体PS I、PS II の分光的解析 (2008)
  • Author(s): 山川壽伯、Md Rafiqul Islam、佐藤和彦、小池裕幸、伊藤繁
  • Organizer: 日本植物学会第72回年会
  • Place of Presentation: 高知大学
• [Presentation] 新型光合成反応中心シアノバクテリアAcaryochloris marina の光化学系Iの機能と電子移動反応 (2008)
  • Author(s): 三野広幸・長井浩子
  • Organizer: 第47回電子スピンサイエンス学会年会
  • Place of Presentation: 九州大学
• [Presentation] 地衣類内共生シアノバクテリアのレーザー共焦点顕微鏡解析I : 地衣4種における地衣組織内分布と色素系比較 (2008)
  • Author(s): 小村理行、佐藤圭介、伊藤繁、岩崎郁子、山本好和
  • Organizer: 第7回日本地衣学会
  • Place of Presentation: 秋田県立大学
• [Presentation] 地衣類内共生シアノバクテリアのレーザー共焦点顕微鏡解析I : 秋田県高尾山と田沢湖で採集したモミジツメゴケ内部と単離培養したNostoc の色素比較 (2008)
  • Author(s): 佐藤圭介、小村理行、岩崎郁子、山本好和、伊藤繁
  • Organizer: 第7回日本地衣学会
  • Place of Presentation: 秋田県立大学
• [Presentation] ジビニルクロロフィルを持つシロイヌナズナ変異体pcb2の光合成能と熱耐性 (2008)
  • Author(s): 山川壽伯、中西弘充、宮本幸一良、林田信明、小村理行、伊藤繁
  • Organizer: 第8回光合成研究会シンポジウム
  • Place of Presentation: 名古屋大学
• [Presentation] Unidirectional electron transfer in photosystem I reaction center of Acarychloris that contain chlorophyll d. (2007)
  • Author(s): Itoh, S., Itoh, K., Mino, M., Kumazaki, S. Iwaki, M.
  • Organizer: 14th International Photosynthesis Congress
  • Place of Presentation: Glasgow, UK
• [Book] 光合成研究法 (2008)
  • Author(s): 伊藤繁
  • Total Pages: 679
  • Publisher: 日本光合成研究会
• [Book] 生物物理学ハンドブック (2007)
  • Author(s): 伊藤繁
  • Total Pages: 680
  • Publisher: 日本生物物理学会
• [Remarks]
  • URL: http://www.bio.nagoya-u.ac.jp/index2.html