Translation arrest and degradation of aberrant protein induced by translation of poly(A) tail
Project/Area Number |
19370075
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Molecular biology
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Research Institution | Nagoya University |
Principal Investigator |
INADA Toshifumi Nagoya University, 大学院・理学研究科, 准教授 (40242812)
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Project Period (FY) |
2007 – 2009
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Project Status |
Completed (Fiscal Year 2009)
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Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2009: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2008: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
|
Keywords | 遺伝子発現 / 品質管理 / 異常mRNA / 異常翻訳 / プロテアソーム / リボソーム / 新生ポリペプチド鎖 / 翻訳アレスト / RNA / 翻訳伸長阻 / RMA品質管理 / タンパク質分解 / mRNA分解 / 翻訳 |
Research Abstract |
Gene expression is highly accurate due to quality-control systems that prevent the expression of potentially harmful protein products. We previously reported that translation of the poly(A) tail plays a crucial role in repressing the production of aberrant proteins from nonstop mRNAs by both translation repression and proteasome-dependent nascent protein destabilization in yeast. This clearly indicates that translation arrest and protein degradation, in addition to mRNA degradation, are crucial for repressing the expression of nonstop mRNAs. We also recently reported that translation arrest caused by the presence of consecutive basic amino acids in a nascent protein induces Not4p-dependent co-translational protein degradation by the proteasome.
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] Nucleolar structure and function regulated by a deubiquitinating enzyme USP362009
Author(s)
Endo, A., Matsumoto, M., Inada, T., Yamamoto, A., Nakayama, K.I., Kitamura, N., Komada, M
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Journal Title
J. Cell Sci 122
Pages: 678-685
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[Journal Article]2009
Author(s)
稲田利文
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Journal Title
稲田利文 (2009) 蛋白質核酸酵素増刊号『多様性と非対称性を獲得するRNAプログラム』(共立出版)
Related Report
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[Journal Article] Nucleolar structure and function regulated by a deubiquitinating enzyme USP362009
Author(s)
Endo, A. Matsumoto, M. Inada, T., Yamamoto, A., Nakayama, K., Kitamura, N, Komada, M.
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Journal Title
J. Cell Sci. 122
Pages: 678-685
Related Report
Peer Reviewed
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[Presentation] RNA制御学2009
Author(s)
稲田利文、大野睦人
Organizer
日本生物化学会シンポジウム
Place of Presentation
神戸
Year and Date
2009-10-22
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