Application and analysis of function of radical scavenger in crop seed germination

• Project/Area Number: 19380014
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Crop science/Weed science
• Research Institution: Kyushu University
• Principal Investigator: IWAYA-INOUE Mari Kyushu University, 農学研究院, 教授 (60091394)
• Co-Investigator(Kenkyū-buntansha): YUASA Takashi 九州大学, 農学研究院, 准教授 (40312269)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000); Fiscal Year 2008: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2007: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
• Keywords: コムギ / 穂発芽 / 自由水 / 過酸化水素 / アスコルビン酸 / ラッカセイ / エチレン

Research Abstract

イネ科およびマメ科作物の子実の成熟および発芽過程について主に活性酸素およびラジカルスカベンジャーの機能に注目して調べた。吸水後のコムギおよびオオムギ種子のアリューロン細胞で生成されるH_2O_2は、抗酸化物質であるアスコルビン酸により消去されることを明らかにした。また、子実の登熟過程において、穂発芽抵抗性のコムギ"農林61号"は、ラジカルスカベンジャーであるアスコルビン酸による発芽抑制効果が登熟後期の開花28 日目から顕著となり、H_2O_2を消去するカタラーゼ活性が著しく高くなった。さらに、コムギのアスコルビン酸散布処理は、いずれの登熟ステージにおいても有効な穂発芽抑制物質となることが示された。デンプン種子だけでなく、油糧種子の成熟や発芽過程についても基礎的な知見を得た。ラッカセイでは、H_2O_2処理により90%以上の発芽率を示し、対照区に比べ著しく改善された。含水率は子葉および下胚軸ともに吸水に伴い漸増したが、下胚軸の増大は顕著だった。水の動態を示すT2は、下胚軸では吸水1日後から5日目まで漸増し自由水が出現した。乾燥子実へのH_2O_2処理により下胚軸および幼根においてエチレン生成が3倍となることが明らかになった。そこで、エチレン応答性遺伝子群の発現を正に制御する転写因子抗GmEin3 抗体によるイムノブロットを行った結果、H_2O_2処理によりラッカセイの下胚軸および幼根に明瞭な交差シグナルが早い時期から検出された。また、ダイズ種子の成熟過程の子実の成熟過程における含水率の変化とオートファジー関連遺伝子であるATG8ファミリーとの関係にも着目し、GmATG8i は含水率の低下に伴い開花28日目からその発現程度が低くなることも合わせて報告した。

Research Products

• [Journal Article] Hydrogen peroxide scavensing regulates germination ability during wheat (Triticum aestivum L.) seed maturation (2008)
  • Author(s): Ishibashi, Y., K. Yamamoto, T. Tawaratsumida, T. Yuasa and M. Iwaya-Inoue
  • Journal Title: Plant Sign. Behav. 3 Pages: 183-188
  • Peer Reviewed
• [Journal Article] Isolation and characterization of a cDNA coding cowpea (Vigna unguiculata (L.) Walp.) calcineurin B-like protein interacting protein kinase (2008)
  • Author(s): Imamura, M., T. Yuasa, T. Takahashi, N. Nakamura, Nang Myint Phyu Sin Htwe, S. H. Zheng , K. Shimazaki, M. Iwaya-Inoue
  • Journal Title: VuCIPK1, Plant Biotechnol 25 Pages: 437-445
  • Peer Reviewed
• [Journal Article] ^1H-NMRによる作物の温度応答モニタリング法 (2008)
  • Author(s): 井上眞理
  • Journal Title: 日本作物学会紀事 77 Pages: 527-532
  • Peer Reviewed
• [Journal Article] Water status of thermal stress-resistant rice seeds (2008)
  • Author(s): Tanaka, K., E. Takeda, R. Onishi, M. Funaba, Y. Ishibashi and M. Iwaya-Inoue
  • Journal Title: Cryobiol. Cryotechnol 54(2) Pages: 129-134
  • Peer Reviewed
• [Journal Article] Relationship of water content and dry weight to autophagy-related gene expression in maturing seeds of soybean (2008)
  • Author(s): Nang Myint Phyu Sin Htwe, H. Tanigawa, Y. Ishibashi, T. Yuasa and M. Iwaya-Inoue
  • Journal Title: Cryobiol. Cryotechnol 54(2) Pages: 135-142
  • Peer Reviewed
• [Journal Article] A tobacco calcium-dependent protein kinase, CDPK1, regulates the transcription factor repression of shoot growth in response to gibberellins (2008)
  • Author(s): Ishiba, S., T. Yuasa, M. Nakata, Y. Takahashi
  • Journal Title: Plant Cell 20(12) Pages: 3273-3288
  • Peer Reviewed
• [Journal Article] Hydrogen peroxide scavensing regulates germination ability during wheat(Triticum aestivum L. )seed maturation. (2008)
  • Author(s): Ishibashi, Y., et al.
  • Journal Title: Plant Sign. Behav. 3 Pages: 183-188
  • Peer Reviewed
• [Journal Article] Isolation and characterization of a cDNA coding cowpea (Vigna unguiculata(L. )Walp. )calcineurin B-like protein interacting protein kinase, VuCIPK1. (2008)
  • Author(s): Imamura, M., et al.
  • Journal Title: Plant Biotechnol. 25 Pages: 437-445
  • Peer Reviewed
• [Journal Article] Nutrient dynamics through leachate and turf grass growth in sands amended with food-waste compost in pots. (2008)
  • Author(s): Shimozono, N., et al.
  • Journal Title: Comm. Soil Sci. Plant Anal. 39 Pages: 241-266
  • Peer Reviewed
• [Journal Article] Water status of thermal stress-resistant rice seeds. (2008)
  • Author(s): Tanaka, K., et al.
  • Journal Title: Cryobiol. Cryotechnol. 54(2) Pages: 129-134
  • Peer Reviewed
• [Journal Article] Relationship of water content and dry weight to autophagy-related gene expression in maturing seeds of soybean. (2008)
  • Author(s): Nang M. P. S. H., et al.
  • Journal Title: Cryobiol. Cryotechnol. 54(2) Pages: 135-142
  • Peer Reviewed
• [Journal Article] A tobacco calcium-dependent protein kinase, CDPK1, regulates the transcription factor repression of shoot growth in response to gibberellins. (2008)
  • Author(s): Ishida, S., et al.
  • Journal Title: Plant Cell 20(12) Pages: 3273-3288
  • Peer Reviewed
• [Journal Article] Hydrogen peroxide scavenging regulates germination ability during wheat(Triticum aestivum L.)seed maturation (2008)
  • Author(s): Ishibashi, Y., et. al.
  • Journal Title: Plant Signaling and Behavior 3(3) Pages: 1-6
  • Peer Reviewed
• [Journal Article] Water status related to aquaporins in cowpea plants exposed to drought stress (2007)
  • Author(s): Yamauchi, T., M. Imamura, M. Arimura, M. Maeshima, R. Matsunaga, T, Yuasa and M. Iwaya-Inoue
  • Journal Title: Cryobiol. Cryotechnol. 53(2) Pages: 87-93
  • Peer Reviewed
• [Journal Article] Effect of low temperature, plant hormones and ascorbic acid on wheat germinationphenomena (2007)
  • Author(s): Tawaratsumida, T., K. Yamamoto, Y. Ishibashi and M. Iwaya-Inoue
  • Journal Title: Cryobiol. Cryotechnol. 53(2) Pages: 133-138
  • Peer Reviewed
• [Journal Article] Water status related to aquaporins in cowpea plants exposed to drought stress (2007)
  • Author(s): Yamauchi, T., et. al.
  • Journal Title: Cryobiology and Cryotechnology 53(2) Pages: 75-85
  • Peer Reviewed
• [Presentation] ラッカセイ子実の発芽時の吸水パターンと中性脂質の分解との関係 (2009)
  • Author(s): 渕上茉莉子、石橋勇志、湯淺高志、井上眞理
  • Organizer: 日本作物学会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2009-03-28
• [Presentation] Starvation stress activates autophagy-related genes and ethylene signaling in soybean seedling (2008)
  • Author(s): Nang Myint Phyu Sin Htwe, T. Yuasa, Y. Hashiguchi, M. Fuchigami and M. Iwaya-Inoue
  • Organizer: Japan-Korea Int. Symposium
  • Place of Presentation: Chungnam natn. Univ., Daejeon, Korea
  • Year and Date: 2008-11-12
• [Presentation] Hydraulic properties of drought-resistant cowpea plants (2008)
  • Author(s): Imamura, M., T. Yuasa and M. Iwaya-Inoue
  • Organizer: Japan-Korea Int. Symposium
  • Place of Presentation: Chungnam natn. Univ., Daejeon, Korea
  • Year and Date: 2008-11-12
• [Presentation] Starvation stress activates autophagy-related genes and ethylene signaling in soybean seedling. (2008)
  • Author(s): Nang, M. P. S. H., et al.
  • Organizer: Japan-Korea Int. Symposium
  • Place of Presentation: Daejeon, Korea
  • Year and Date: 2008-11-12
• [Presentation] Hydraulic properties of drought-resistant cowpea plants. (2008)
  • Author(s): Imamura, M., et al.
  • Organizer: Japan-Korea Int. Symposium
  • Place of Presentation: Daejeon, Korea
  • Year and Date: 2008-11-12
• [Presentation] ダイズとインゲンマメの発芽過程における水分動態と組織化学的特性 (2008)
  • Author(s): 久松美咲、谷川宏行、石橋勇志、湯浅高志、井上眞理
  • Organizer: 日本作物学会
  • Place of Presentation: 神戸大学
  • Year and Date: 2008-09-24
• [Presentation] コムギの登熟過程および成熟子実に対するアスコルビン酸の発芽抑制効果 (2008)
  • Author(s): 山本康平、俵積田智也、石橋勇志、湯浅高志、井上眞理
  • Organizer: 日本作物学会
  • Place of Presentation: 神戸大学
  • Year and Date: 2008-09-24
• [Presentation] 乾燥ストレスによるササゲの子実成熟期における葉と子実の糖動態の変化 (2008)
  • Author(s): 有村恵, ほか
  • Organizer: 日本作物学会
  • Place of Presentation: 神戸大学
  • Year and Date: 2008-09-24
• [Presentation] マメ科子実の発芽特性(水分動態と組織化学的解析) (2008)
  • Author(s): 久松美咲, ほか
  • Organizer: 日本作物学会九州支部会
  • Place of Presentation: 熊本県立大学
  • Year and Date: 2008-08-20
• [Presentation] Role of hydrogen peroxide produced in aleurone cell on germination process (2008)
  • Author(s): Ishibashi, Y., T. Tawaratsumida, K. Kondou, T. Yuasa and M. Iwaya-Inoue
  • Organizer: The 9th ISSS Conference on Seed Biology.
  • Place of Presentation: The University of Warmia and Mazury Olsztyn, Poland
  • Year and Date: 2008-06-11
• [Presentation] Reactive oxygen species produced by NADPH oxidase are involved in promoting the germination of barley seeds (2008)
  • Author(s): Tawaratsumida, T., K. Kondou, Y. Ishibashi, T. Yuasa and M. Iwaya-Inoue
  • Organizer: The 9th ISSS Conference on Seed Biology.
  • Place of Presentation: The University of Warmia and Mazury Olsztyn, Poland
  • Year and Date: 2008-06-11
• [Presentation] Role of hydrogen peroxide produced in aleurone cell on germination process. (2008)
  • Author(s): Ishibashi, Y., et al.
  • Organizer: The 9th ISSS Conference on Seed Biology
  • Place of Presentation: Olsztyn, Poland
  • Year and Date: 2008-06-11
• [Presentation] Reactive oxygen species produced by NADPH oxidase are involved in promoting the germination of barley seeds. (2008)
  • Author(s): Tawaratsumida, T., et al.
  • Organizer: The 9th ISSS Conference on Seed Biology
  • Place of Presentation: Olsztyn, Poland
  • Year and Date: 2008-06-11
• [Presentation] Relationship between H_2O_2 scavenging and germination ability during wheat (Triticum aestivum L.) (2008)
  • Author(s): Ishibashi, Y., K. Yamamoto, T. Tawaratsumida, T. Yuasa and M. Iwaya-Inoue
  • Organizer: The 5th Int. Crop Science Congress
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2008-04-13
• [Presentation] The influence on germination in wheat seeds treated by plant hormones, ascorbic acid and temperatures in relation to water status (2008)
  • Author(s): Tawaratsumida, T., K. Yamamoto, Y. Ishibashi and M. Iwaya-Inoue
  • Organizer: The 5th Int. Crop Science Congress
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2008-04-13
• [Presentation] Influences of heat stress on rice plants characterized by induction of heat shock proteins in relation to water status (2008)
  • Author(s): Tanaka, K., E. Takeda, R. Onishi, Y. Ishibashi, T. Yuasa and M. Iwaya-Inoue
  • Organizer: The 5th Int. Crop Science Congress
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2008-04-13
• [Presentation] Relationship between H_2O_2 scavenging and germination ability during wheat (Triticum aestivum L. ). (2008)
  • Author(s): Ishibashi, Y., et al.
  • Organizer: The 5th Int. Crop Science Congress
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2008-04-13
• [Presentation] The Influence on germination in wheat seeds treated by plant hormones ascorbic acid and temperatures in relation to water status. (2008)
  • Author(s): Tawaratsumida, T., et al.
  • Organizer: The 5th Int. Crop Science Congress
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2008-04-13
• [Presentation] Influences of heat stress on rice plants characterized by induction of heat shock proteins in relation to water status. (2008)
  • Author(s): Tanaka, K., et al.
  • Organizer: The 5th Int. Crop Science Congress
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2008-04-13
• [Presentation] 水稲登熟初期の高温ストレスに対する籾の水分動態 (2008)
  • Author(s): 田中浩平、大西亮樹、武田恵理子、井上眞理
  • Organizer: 日本作物学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-03-27
• [Presentation] 登熟過程における「にこまる」と「ヒノヒカリ」籾の温度ストレス応答の差異 (2008)
  • Author(s): 舩場貢、武田恵理子、石橋勇志、井上眞理
  • Organizer: 日本作物学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-03-27
• [Presentation] トマトのSNF1関連キナーゼホモログと環境ストレスシグナル (2008)
  • Author(s): 湯浅 高志
  • Organizer: 日本植物生理学会
  • Place of Presentation: 札幌コンベンションセンター
• [Presentation] 登熟過程における「にこまる」と「ヒノニカリ」籾の温度ストレス応答の差異 (2008)
  • Author(s): 舩場 貢
  • Organizer: 日本作物学会
  • Place of Presentation: 農林水産技術会議事務所
• [Presentation] 水稲登熟初期の高温ストレスに対する籾の水分動態 (2008)
  • Author(s): 田中 浩平
  • Organizer: 日本作物学会
  • Place of Presentation: 農林水産技術会議事務所
• [Presentation] コムギの登熟過程の発芽現象における低温とアスコルビン酸の拮抗作用 (2007)
  • Author(s): 俵積田智也、山本康平、石橋勇志、井上眞理
  • Organizer: 低温生物工学会大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2007-07-23
• [Presentation] イネの登熟過程における温度ストレスが子実の形態形成に及ぼす水分生理学的要因 (2007)
  • Author(s): 武田恵理子、舩場貢、岩浪賢司、伊佐磨美、石橋勇志、井上眞理
  • Organizer: 日本植物学会九州支部会
  • Place of Presentation: 福岡女子大学
  • Year and Date: 2007-05-08
• [Presentation] ササゲの気孔閉鎖とABA 合成関連遺伝子の発現と関係について (2007)
  • Author(s): 今村雅和、山内崇、古屋忠彦、湯淺高志、井上眞理
  • Organizer: 日本作物学会
  • Place of Presentation: 金沢大学
• [Presentation] マメ科植物のCBL結合プロテインキナーゼ(VuCIPK1)の解析 (2007)
  • Author(s): 今村 雅和
  • Organizer: 日本植物学会
  • Place of Presentation: 東京理科大学
• [Presentation] ダイズのカルシニューリンB様分子結合プロテインキナーゼおよびCBLホモログの解析 (2007)
  • Author(s): 湯浅 高志
  • Organizer: 日本作物学会
  • Place of Presentation: 金沢大学
• [Remarks]
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• [Remarks]
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