| Project/Area Number |
19380019
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Kyoto University |
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Principal Investigator |
YONEMORI Keizo Kyoto University, 農学研究科, 教授 (10111949)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Masahiko 果樹研究所, ブドウ・カキ研究チーム, 上席研究員 (00355439)
SATO Akihiko 果樹研究所, ブドウ・カキ研究チーム, 上席研究員 (30355440)
TAO Ryutaro 京都大学, 農学研究科, 准教授 (10211997)
YAMANE Hisayo 京都大学, 農学研究科, 助教 (80335306)
北島 宣 近畿大学, 農学部, 講師 (70135549)
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| Co-Investigator(Renkei-kenkyūsha) |
KANNZAKI Shinya 近畿大学, 農学部, 講師 (20330243)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2007: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
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| Keywords | マメガキ / 倍数体 / 甘渋性決定遺伝子 / 転写因子 / 定量PCR / DNAマーカー / 遺伝子単離 / プロアントシアニジン / AFLP / 羅田甜柿 / 甘渋性関与遺伝子 / 遺伝子発現 / フラボノイド合成 / タンニン細胞 |
|---|
| Research Abstract |
The gene conferring the trait of astringency-loss in Japanese-type PCNA is shown to be located at 100kb away of fosmid contig constructed from Diospyros lotus, a close relative of D.kaki. This single gene controls the astringency trait in persimmon fruit and 6 alleles are responsible for the appearance of this trait. The DkMyb4, a regulatory gene, is also found to control tannin biosynthesis in the fruit and is indicated to determine whether an individual is PCNA or non-PCNA due to its strength of expression. Using the DNA sequences linked to this gene, we could construct a primer pair for PCR analysis for selecting PCNA offspring of Japanese-type. In addition, we also found an AFLP marker to distinguish Chinese-type PCNA among breeding population.
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