Studies on cellular function of C1-microorganisms aiming at reduction of green house gas

• Project/Area Number: 19380048
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kyoto University
• Principal Investigator: SAKAI Yasuyoshi Kyoto University, 大学院・農学研究科, 教授 (60202082)
• Co-Investigator(Kenkyū-buntansha): YURIMOTO Hiroya 京都大学, 大学院・農学研究科, 准教授 (00283648)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000); Fiscal Year 2008: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000); Fiscal Year 2007: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
• Keywords: メタン / メタノール / メタン資化性細菌 / メタノール資化性細菌 / メタノール資化性酵母 / コンソーシアム / ホルムアルデヒド / ETBE

Research Abstract

植物表層におけるメタンやメタノール資化性微生物の分布や生理生態を明らかにするとともに、温室効果ガス排出削減やこれらの炭素源を資源化するために必要な応用微生物学研究を行った。植物試料を分離源にメタンおよびメタノール資化性微生物のスクリーニングを行い、多くの分離源からメタン資化性細菌とメタノール資化性細菌を含む微生物コンソーシアムを取得した。また、葉上でのメタノール資化性酵母の生育にはメタノール代謝経路が重要であること、植物葉上には大量の微生物が利用可能なメタノールが存在することが明らかとなってきた。

Research Products

• [Journal Article] Activation of the oxidative stress regulator PpYap1 through conserved cysteine residues during methanol metabolism in the yeast Pichia pastoris (2009)
  • Author(s): Yano, T., H. Yurimoto, and Y. Sakai
  • Journal Title: Biosci. Biotechnol. Biochem (in press)
  • NAID: 10027542721
  • Peer Reviewed
• [Journal Article] Atg26-mediated pexophagy is required for host invasion by the plant pathogenic fungus Colletotrichum orbiculare (2009)
  • Author(s): Asakura, M., S. Ninomiya, M. Sugimoto,M. Oku, S.Yamashita, T. Okuno, Y.Sakai, and Y. Takano
  • Journal Title: Plant Cell 21 Pages: 1291-1304
  • Peer Reviewed
• [Journal Article] The Yap-1 regulated glutathione redox system curtails the accumulation of formaldehyde and reactive oxygen species in methanol metabolism of Pichia pastoris (2009)
  • Author(s): Yano, T., E. Takigami, H. Yurimoto and Y. Sakai
  • Journal Title: Eukaryot. Cell 8 Pages: 540-549
  • Peer Reviewed
• [Journal Article] ethanolinducible gene expression and heterologous protein production in the methylotrophic yeast Candida boidinii (2009)
  • Author(s): Yurimoto H. and Y. Sakai
  • Journal Title: Biotechnol. Appl. Biochem 53 Pages: 85-92
  • Peer Reviewed
• [Journal Article] The Yapl-regulated glutathione redox system curtails the accumulation of formaldehyde and reactive oxygen species in methanol metabolism of Pichia nastoris (2009)
  • Author(s): Yano, T., et . al.
  • Journal Title: Eukaryot. Cell 8 Pages: 540-549
  • Peer Reviewed
• [Journal Article] Activation of the oxidative stress regulator PpYapl through conserved cysteine residues during methanol metabolism in the Pichia pastoris (2009)
  • Author(s): Yano, T., et . al.
  • Journal Title: Biosci. Biotechnol. Biochem.
  • Peer Reviewed
• [Journal Article] Methanol-inducible gene expression and heterologous protein production in the methylotrophic yeast Candida boidinii. (2009)
  • Author(s): Yurimoto, H., Sakai, Y.
  • Journal Title: Biotechnol. Appl. Biochem.
  • Peer Reviewed
• [Journal Article] Atg26-mediated pexophagy is required for host invasion by the plant pathogenic fungus Colletotrichum orbiculare. (2009)
  • Author(s): Asakura, M., et . al.
  • Journal Title: Plant Cell
  • Peer Reviewed
• [Journal Article] アーキアにおけるホルムアルデヒド固定酵素群の生理機能 (2008)
  • Author(s): 由里本博也、折田和泉、阪井康能
  • Journal Title: バイオサイエンスとインダストリー 66 Pages: 447-449
  • NAID: 10024745583
• [Journal Article] Trm1p, a Zn(II)_2Cys_6-type transcription factor, is a master regulator of methanol-specific gene activation in the methylotrophic yeast Candida boidinii (2008)
  • Author(s): Sasano, Y., H. Yurimoto, M. Yanaka, and Y. Sakai
  • Journal Title: Eukaryot. Cell 7 Pages: 527-536
  • Peer Reviewed
• [Journal Article] アーキアにおけるホルムアルデヒド固定酵素群の生理機能 (2008)
  • Author(s): 由里本博也, 折田和泉, 阪井康能
  • Journal Title: バイオサイエンスとインダストリー 66 Pages: 447-449
  • NAID: 10024745583
• [Journal Article] Trmlp, a Zn(II)2Cys6-type transcription factor, is a master regulator of methanol-specific gene activation in the methylotrophic yeast Candida boidinii (2008)
  • Author(s): Sasano, Y., et. al.
  • Journal Title: Eukaryot. Cell. 7 Pages: 527-536
  • Peer Reviewed
• [Journal Article] Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase (2007)
  • Author(s): Orita, I., N. Sakamoto, N. Kato, H.Yurimoto, and Y. Sakai
  • Journal Title: Appl. Microbiol.Biotechnol 76 Pages: 439-445
  • Peer Reviewed
• [Journal Article] Gene-tagging mutagenesis in the methylotrophic yeast Candida boidinii (2007)
  • Author(s): Sasano, Y., H. Yurimoto, and Y. Sakai
  • Journal Title: J. Biosci. Bioeng 104 Pages: 86-89
  • NAID: 110006368813
  • Peer Reviewed
• [Journal Article] Gene-tagging mutagenesis in the methylotrophic yeast Candida boidinii (2007)
  • Author(s): Sasano, Y., et. al.
  • Journal Title: J. Biosci. Bioeng. 104 Pages: 86-89
  • Peer Reviewed
• [Journal Article] Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase (2007)
  • Author(s): Orita, I., et. al.
  • Journal Title: Appl. Microbiol. Biotechnol. 76 Pages: 439-445
  • Peer Reviewed
• [Presentation] Importance of glutathione redox system and its regulation in yeast methanol metabolism (2008)
  • Author(s): Yano, T., E. Takigami, H. Yurimoto, Y. Sakai
  • Organizer: The 12th International Congress on Yeasts
  • Place of Presentation: Kyiv, Ukraine
• [Presentation] Syntrophic life style of C1-microorganisms on plant leaves (2008)
  • Author(s): Sakai, Y.
  • Organizer: Gordon Research Conference on Molecular Basis Of Microbial One-Carbon Metabolism
  • Place of Presentation: Lewiston, ME, USA
• [Presentation] Physiological role of RuMP pathway enzymes and their application (2008)
  • Author(s): Yurimoto, H.
  • Organizer: Gordon Research Conference on Molecular Basis Of Microbial One Carbon Metabolism
  • Place of Presentation: Lewiston, ME, USA