Elucidation of the molecular mechanism underlying the enhancement of somatic-cell reprogramming by a histone deacetylase inhibitor, and its applications
| Project/Area Number |
19380161
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kinki University |
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Principal Investigator |
KISHIGAMI Satoshi Kinki University, 生物理工学部, 准教授 (10291064)
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| Research Collaborator |
TERUHIKO Wakayama 理化学研究所・神戸研究所, チームリーダー (40360672)
KAZUHIRO Saeki 近畿大学, 生物理工学部, 教授 (10298937)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2007: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Keywords | 発生工学 / 核の初期化 / 核移植 / リプログラミング / クローン動物 / ヒストン脱アセチル化酵素 / マウス / 再生医療 / ピストン脱アセチル化酵素 |
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| Research Abstract |
Somatic-cell nuclear transfer (SCNT) has been extremely inefficient with rates less than 5 percentage points since the first clone, "Dolly" the sheep. The representative researcher for this grant found that treatment of cloned embryos with trichostatin A (TSA), a histone deacetylase inhibitor (HDACi), dramatically increase the production rate of cloned mice more than six-times. Supported by this grant, the importance of HDAC in embryonic development and a mechanism underlying improvement of SCNT by HDACi treatment were revealed and, further cloning of "unclonable" mouse strains were achieved for the first time.
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Report
(4 results)
Research Products
(62 results)
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[Journal Article]2009
Author(s)
岸上哲士, 矢持隆之, 松原圭吾, 細井美彦
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Journal Title
再生医療(メディカルレビュー社)
Pages: 43-46
Related Report
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[Journal Article] Regulation of chromatin and chromosome morphology by histone H3 modifications in pig oocytes2007
Author(s)
Bui HT, Van Thuan N, Kishigami S, Wakayama S, Hikichi T, Ohta H, Mizutani E, Yamaoka E, Wakayama T, Miyano T
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Journal Title
Reproduction Vol.133,No.2
Pages: 371-382
Related Report
Peer Reviewed
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[Journal Article] Differentiation potential of parthenogenetic embryonic stem cells is improved by nuclear transfer2007
Author(s)
Hikichi T, Wakayama S, Mizutani E, Takashima Y, Kishigami S, Van Thuan N, Ohta H, Thuy Bui H, Nishikawa S, Wakayama T
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Journal Title
Stem Cells Vol.25,No.1
Pages: 46-53
Related Report
Peer Reviewed
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