Molecular basis of pathogenicity of classical swine fever virus -Mechanism of down regulation of innate immunity in the cells and pigs infected with classical swine fever virus-

• Project/Area Number: 19380170
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Hokkaido University
• Principal Investigator: SAKODA Yoshihiro Hokkaido University, 大学院・獣医学研究科, 准教授 (40333637)
• Co-Investigator(Kenkyū-buntansha): AOKI Hiroshi 日本獣医生命科学大学, 獣医学部, 講師 (10440067)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2007: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: 豚コレラウイルス / 病原性 / 自然免疫 / ペスチウイルス / 宿主

Research Abstract

To assess the molecular basis of classical swine fever virus, the mechanism of down regulation of innate immunity mediated by type-1 interferon was analyzed using mutant viruses constructed by reverse genetics. Critical amino acids of N^<pro> protein for the control of type-1 interferon production were determined and it was concluded that the down regulation of innate immunity mediated by type-1 interferon was one of the important factors for the pathogenicity of classical swine fever virus in pigs.

Research Products

• [Journal Article] Classical swine fever virus can remain virulent after specific elimination of the interferon regulatory factor 3-degrading function of Npro (2009)
  • Author(s): Ruggli, N., Summerfield, A., Fiebach, A.R., Guzylack-Piriou, L., Bauhofer, O., Lamm, C.G., Waltersperger, S., Matsuno, K., Liu, L., Gerber, M., Choi, K.H., Hofmann, M.A., Sakoda, Y., Tratschin, J. D
  • Journal Title: J. Virol 83 Pages: 817-829
  • Peer Reviewed
• [Journal Article] Classical swine fever virus can remain virulent after specific elimination of the interferon regulatory factor 3-degrading function of Npro (2009)
  • Author(s): Ruggli, N., et al
  • Journal Title: J.Virol 83 Pages: 817-829
  • Peer Reviewed
• [Journal Article] 豚コレラ清浄性維持確認のための診断技術 (2009)
  • Author(s): 青木博史
  • Journal Title: 養豚の友 479 Pages: 24-29
• [Journal Article] Cleavage of the NS2-3 protein in the cells of cattle persistently infected with non-cytopathogenic bovine viral diarrhea virus (2008)
  • Author(s): Kameyama, K., Sakoda, Y., Matsuno, K., Ito, A., Tajima, M., Nakamura, S., Kida, H
  • Journal Title: Microbiol. Immunol 52 Pages: 277-282
  • NAID: 10025691963
  • Peer Reviewed
• [Journal Article] Cleavage of the NS2-3 protein in the cells of cattle persistently infected with non-cytopathogenic bovine viral diarrhea virus (2008)
  • Author(s): Kameyama, K., et.al.
  • Journal Title: Microbiol Immunol 52 Pages: 277-282
  • Peer Reviewed
• [Journal Article] Identification of new genetic subtypes of bovine viral diarrhea virus genotype 1 isolated in Japan (2008)
  • Author(s): Nagai, M., et.al.
  • Journal Title: Virus Genes 36 Pages: 135-139
  • Peer Reviewed
• [Journal Article] Classical swine fever virus can remain virulent after specific elimination of the interferon regulatory factor 3 degrading function of Npro (2008)
  • Author(s): Ruggli, N., et.al.
  • Journal Title: J Virol 83 Pages: 817-829
  • Peer Reviewed
• [Journal Article] 豚コレラ清浄性維持確認に求められる豚コレラ診断法について (2008)
  • Author(s): 青木博史
  • Journal Title: 家畜衛生雑誌 34(3) Pages: 109-114
• [Journal Article] 日本における豚コレラの撲滅達成と今後 (2008)
  • Author(s): 青木博史
  • Journal Title: 獣医疫学雑誌 12(2) Pages: 120-124
  • NAID: 130000340450
• [Journal Article] Identification of new genetic subtypes of bovine viral diarrhea virus genotype 1 isolated in Japan. (2008)
  • Author(s): Nagai, M., et. al.
  • Journal Title: Virus Genes 36 Pages: 135-139
  • Peer Reviewed
• [Journal Article] Cleavage of the NS2-3 protein in the cells of cattle persistent ly infected with non-cytopathogenic bovine viral diarrhea virus. (2008)
  • Author(s): Kameyama, K., et. al.
  • Journal Title: Microbiol Immunol (In press)
  • Peer Reviewed
• [Journal Article] Genomic analyses of bovine viral diarrhea viruses isolated form imported cattle between 1991 and 2005. (2008)
  • Author(s): Yamamoto, T., et. al.
  • Journal Title: Veterinary Microbiology 127 Pages: 386-391
  • Peer Reviewed
• [Journal Article] Classical swine fever virus Npro interacts with interferon regulatory factor 3 and induces its proteasomal degradation (2007)
  • Author(s): Bauhofer, O., Summerfield, A., Sakoda, Y., Tratschin, J.D., Hofmann, M.A., Ruggli, N
  • Journal Title: J. Virol 81 Pages: 3087-3096
  • Peer Reviewed
• [Journal Article] Classical swine fever virus N^<pro> interacts with interferon regulatory factor 3 and induces its proteasomal degradation. (2007)
  • Author(s): Bauhofer, O., et. al.
  • Journal Title: Journal of Virology 81 Pages: 3087-3096
  • Peer Reviewed
• [Journal Article] Genetic and pathobiological characterization of bovine viral diarrhea viruses recently isolated from cattle in Japan. (2007)
  • Author(s): Matsuno, K., et. al.
  • Journal Title: Journal of Veterinary Medical Science 69 Pages: 515-520
  • Peer Reviewed
• [Presentation] 豚コレラ弱毒生ワクチン株の豚扁桃継代による病原性の復帰 (2010)
  • Author(s): 吉野史、迫田義博、杉田征彦、野村拓志、山本直樹、岡松正敏、喜田宏
  • Organizer: 第149回日本獣医学会
  • Place of Presentation: 日本獣医生命科学大学、東京
  • Year and Date: 2010-03-26
• [Presentation] 細胞病原性牛ウイルス性下痢ウイルスNose株に含まれる費細胞病原性準種の遺伝子構造解析 (2010)
  • Author(s): 青木博史
  • Organizer: 第149回日本獣医学会
  • Place of Presentation: 日本獣医生命科学大学、東京
  • Year and Date: 2010-03-26
• [Presentation] 細胞病原性牛ウイルス性下痢ウイルスNose株に含まれる非細胞病原性準種の遺伝子構造解析 (2010)
  • Author(s): 青木博史
  • Organizer: 第149回日本獣医学会
  • Place of Presentation: 日本獣医生命科学大学、東京
  • Year and Date: 2010-03-26
• [Presentation] 近年北海道で分離された牛ウイルス性下痢ウイルスの遺伝子解析 (2010)
  • Author(s): 田村友和, ら
  • Organizer: 第149回日本獣医学会
  • Place of Presentation: 日本獣医生命科学大学、東京
  • Year and Date: 2010-03-26
• [Presentation] 牛ウイルス性下痢ウイルス(BVDV)の表面糖蛋白E2に対するモノクローン抗体の作出と抗原性解析 (2010)
  • Author(s): 杉田征彦, ら
  • Organizer: 第149回日本獣医学会
  • Place of Presentation: 日本獣医生命科学大学、東京
  • Year and Date: 2010-03-26
• [Presentation] 豚コレラウイルスの病原性の分子基盤-弱毒生ワクチン株の豚継代による病原性の部分復帰- (2009)
  • Author(s): 迫田義博、岡松正敏、野村拓志、杉田征彦、吉野史、喜田宏
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 都市センターホテル、東京
• [Presentation] Induction of apoptosis in porcine kidney cells by type I interferon induced by infection with classical swine fever virus (2008)
  • Author(s): Sakoda Y, Ito A, Kameyama K, Nomura T, Ruggli N, Tratschin J. -D, Kida H
  • Organizer: The 7th ESVV Pestivirus Symposium
  • Place of Presentation: Uppsala, Sweden
• [Presentation] Expression kinetics of Interferon Promoter Stimulator-1(IPS-1) inducing natural immunity in the cells infected with Classical Swine Fever Virus (2008)
  • Author(s): Nomura T, Sakoda Y, Matsuno K, Kida H
  • Organizer: 7th ESVV Pestivirus Symposium
  • Place of Presentation: Uppsala, Sweden
• [Presentation] 豚コレラウイルス感染細胞内における自然免疫関連因子Interferon Promoter Stimulator-1(IPS-1)の動態 (2008)
  • Author(s): 野村拓志, ら
  • Organizer: 第73回日本インターフェロン・サイトカイン学会学術集会・第19回日本生体防御学会学術総会・第45回補体シンポジウム合同大会
  • Place of Presentation: 北海道大学、札幌
• [Presentation] 豚コレラウイルスの感染より誘導されたI型インターフェロンが直接豚腎臓細胞のアポトーシスを誘導する (2008)
  • Author(s): 迫田義博, ら
  • Organizer: 第73回日本インターフェロン・サイトカイン学会学術集会・第19回日本生体防御学会学術総会・第45回補体シンポジウム合同大会
  • Place of Presentation: 北海道大学、札幌
• [Presentation] 動物用生物学的製剤の迷入ウイルス否定試験に供するための牛胎子血清の品質調査 (2008)
  • Author(s): 小佐々隆志, ら
  • Organizer: 第56回日本ウィルス学会
  • Place of Presentation: 岡山コンベンションセンター、岡山
• [Presentation] Induction of apoptosis in porcine kidney cells by type I interferoninduced by infection with classical swine fever virus (2008)
  • Author(s): Sakoda, Y., et.al.
  • Organizer: The 7th ESVV Pestivirus Symposium
  • Place of Presentation: Uppsala, Sweden
• [Presentation] Expression kinetics of Interferon Promoter Stimulator-1 (IPS-1) inducing natural immunity in the cells infected with Classical Swine Fever Virus (2008)
  • Author(s): Nomura, T., et.al.
  • Organizer: The 7th ESVV Pestivirus Symposium
  • Place of Presentation: Uppsala, Sweden
• [Presentation] 豚コレラウイルスのCPK-NS細胞に対する病原性の解析 (2007)
  • Author(s): 伊藤麻子、迫田義博、Nicolas Ruggli、喜田宏
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 札幌コンベンションセンター、札幌
• [Presentation] 牛ウイルス性下痢ウイルスの生物現象の差によるゲノム塩基配列の相違 (2007)
  • Author(s): 小佐々隆志、青木博史、野田紗知子、関口秀人、福所秋雄、中村成幸
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 札幌コンベンションセンター、札幌
• [Presentation] 豚コレラウイルスのCPK-NS細胞に対する病原性の解析 (2007)
  • Author(s): 伊藤麻子、迫田義博、Nicolas Ruggli、喜田宏
  • Organizer: 第144回日本獣医学会学術集会
  • Place of Presentation: 酪農学園大学、江別
• [Presentation] 豚コレラウイルス感染細胞内における自然免疫関連因子Interferon Promoter Stimulator-1(IPS-1)の動態 (2007)
  • Author(s): 野村拓志, ら
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 札幌コンベンションセンター、札幌
• [Presentation] 豚コレラウイルス感染細胞内における自然免疫関連因子Interferon Promoter Stimulator-1(IPS-1)の勲態 (2007)
  • Author(s): 野村拓志, ら
  • Organizer: 第144回日本獣医学会学術集会
  • Place of Presentation: 酪農学園大学、江別
• [Presentation] END現象の解明に向けた牛ウイルス性下痢ウイルスのEND陽性株および陰性株の遺伝子解析 (2007)
  • Author(s): 小佐々隆志, ら
  • Organizer: 第143回日本獣医学会学術集会
  • Place of Presentation: っくば国際会議場、つくば