Elucidation of function of polyamines and regulation of their contents in cells
Project/Area Number |
19390016
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biological pharmacy
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Research Institution | Chiba University |
Principal Investigator |
IGARASHI Kazuei Chiba University, 名誉教授 (60089597)
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Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Kazuhiro 千葉大学, 大学院・薬学研究院, 講師 (60302569)
KASHIWAGI Keiko 千葉科学大学, 薬学部, 教授 (80169424)
植村 武史 千葉大学, 大学院・薬学研究院, 助教 (50401005)
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Project Period (FY) |
2007 – 2009
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Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2008: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2007: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
|
Keywords | 核酸 / 生体分子 / 生理活性 / 脳・神経 / バイオテクノロジー |
Research Abstract |
Polyamines (putrescine, spermidine, spermine) play important roles in cell proliferation and differentiation. In this study, role of polyamines on stimulation of specific kinds of protein synthesis and NMDA receptor activity at both stimulatory and resting states, and properties of polyamine transport systems in Escherichia coli and yeast have been studied. New findings in this study have been described below.
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Report
(4 results)
Research Products
(78 results)
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[Journal Article] Intense correlation between protein-conjugated acrolein and primary Sjogren's syndrome2010
Author(s)
Higashi, K., Yoshida, M., Igarashi, A., Ito, K., Wada, Y., Murakami, S., Kobayashi, D., Nakano, M., Sohda, M., Nakajima, T., Narita, I., Toida, T., Kashiwagi, K., Igarashi, K.
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Journal Title
Clin.Chim.Acta 41
Pages: 359-363
Related Report
Peer Reviewed
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[Journal Article] Identification of acrolein-conjugated protein in plasma of patients with brain infarction2010
Author(s)
Yoshida, M., Higashi, K., Jin, L., Machi, Y., Suzuki, T., Masuda, A., Dohmae, N., Suganami, A., Tamura, Y., Nishimura, K., Toida, T., Tomitori, H., Kashiwagi, K., Igarashi, K.
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Journal Title
Biochem.Biophys.Res.Commun. 391
Pages: 1234-1239
Related Report
Peer Reviewed
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[Journal Article] Polyamine-modulon in yeast―stimulation of COX4 synthesis by spermidine at the level of translation2009
Author(s)
Uemura, T., Higashi, K., Takigawa, M., Toida, T., Kashiwagi, K., Igarashi, K.
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Journal Title
Int.J.Biochem.Cell Biol. 41
Pages: 2538-2545
Related Report
Peer Reviewed
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[Journal Article] Intense correlation between brain infarction and protein-conjugated acrolein2009
Author(s)
Saiki, R., Nishimura, K., Ishii, I., Omura, T., Okuyama, S., Kashiwagi, K., Igarashi, K.
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Journal Title
Stroke 40
Pages: 3356-3361
Related Report
Peer Reviewed
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[Journal Article] Acrolein, IL-6 and CRP as markers of silent brain infarction2009
Author(s)
Yoshida, M., Tomitori, H., Machi, Y., Katagiri, D., Ueda, S., Horiguchi, K., Kobayashi, E., Saeki, N., Nishimura, K., Ishii, I., Kashiwagi, K., Igarashi, K.
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Journal Title
Atherosclerosis 203
Pages: 557-562
Related Report
Peer Reviewed
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[Journal Article] Acrolein toxicity : comparison with reactive oxygen species2009
Author(s)
Yoshida, M., Tomitori, H., Machi, Y., Hagihara, M., Higashi, K., Goda, H., Ohya, T., Niitsu, M., Kashiwagi, K., Igarashi, K.
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Journal Title
Biochem.Biophys.Res.Commun. 378
Pages: 313-318
Related Report
Peer Reviewed
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[Journal Article] Identification of proteins whose synthesis is preferentially enhanced by polyamines at the level of translation in mammalian cells2009
Author(s)
Nishimura, K., Okudaira, H., Ochiai, E., Higashi, K., Kaneko, M., Ishii, I., Nishimura, T., Dohmae, N., Kashiwagi, K., Igarashi, K.
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Journal Title
Int.J.Biochem.Cell Biol. 41
Pages: 2251-2261
Related Report
Peer Reviewed
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[Journal Article] Enhancement of the synthesis of RpoE and StpA by polyamines at the level of translation in Escherichia coli under heat shock conditions2009
Author(s)
Terui, Y., Higashi, K., Tabei, Y., Tomitori, H., Yamamoto, K., Ishihama, A., Igarashi, K., Kashiwagi, K.
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Journal Title
J.Bacteriol. 191
Pages: 5348-5357
Related Report
Peer Reviewed
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[Journal Article] Selective structural change by spermidine of the bulged-out region of double-stranded RNA and its effect on RNA function2008
Author(s)
Higashi, K., Terui, Y., Suganami, A., Tamura, Y., Nishimura, K., Kashiwagi, K., Igarashi, K.
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Journal Title
J.Biol.Chem. 283
Pages: 32989-32994
Related Report
Peer Reviewed
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[Journal Article] The pore region of N-methyl-D-aspartate receptors differentially influences stimulation and block by spermine2008
Author(s)
Jin, L., Miyazaki, M., Mizuno, S., Takigawa, M., Hirose, T., Nishimura, K., Toida, T., Williams, K., Kashiwagi, K., Igarashi, K.
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Journal Title
J.Pharmacol.Exp.Ther. 327
Pages: 68-77
Related Report
Peer Reviewed
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[Journal Article] Selective structural change of bulged-out region of double-stranded RNA containing bulged nucleotides by spermidine2008
Author(s)
Higashi, K., Terui, Y., Inomata, E., Katagiri, D., Nomura, Y., Someya, T., Nishimura, K., Kashiwagi, K., Kawai, G., Igarashi, K.
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Journal Title
Biochem.Biophys.Res.Commun. 370
Pages: 572-577
Related Report
Peer Reviewed
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[Journal Article] Binding of spermine and ifenprodil to a purified, soluble regulatory domain of the N-methyl-Daspartate receptor2008
Author(s)
Han, X., Tomitori, H., Mizuno, S., Higashi, K., Full, C., Fukiwake, T., Terui, Y., Leewanich, P., Nishimura, K., Toida, T., Williams, K., Kashiwagi, K., Igarashi, K.
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Journal Title
J.Neurochem. 107
Pages: 1566-1577
Related Report
Peer Reviewed
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[Journal Article] Identification of a spermidine excretion protein complex (MdtJI) in Escherichia coli2008
Author(s)
Higashi, K., Ishigure, H., Demizu, R., Uemura, T., Nishino, K., Yamaguchi, A., Kashiwagi, K., Igarashi, K.
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Journal Title
J.Bacteriol. 190
Pages: 872-878
Related Report
Peer Reviewed
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[Journal Article] Enhancement of the synthesis of RpoN, Cra and H-NS by polyamines at the level of translation in Escherichia coli cultured with glucose and glutamate2007
Author(s)
Terui, Y., Higashi, K., Taniguchi, S., Shigemasa, A., Nishimura, K., Yamamoto, K., Kashiwagi, K., Ishihama, A., Igarashi, K.
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Journal Title
J.Bacteriol. 189
Pages: 2359-2368
Related Report
Peer Reviewed
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[Journal Article] Comparative studies of anthraquinone-and anthracenetetraamines as blockers of N-methyl-D-aspartate receptors2007
Author(s)
Jin, L., Sugiyama, H., Takigawa, M., Katagiri, D., Tomitori, H., Nishimura, K., Kaur, N., Phanstiel IV, O., Kitajima, M., Takayama, H., Okawara, T., Williams, K., Kashiwagi, K., Igarashi, K.
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Journal Title
J.Pharmacol.Exp.Ther. 320
Pages: 47-55
Related Report
Peer Reviewed
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[Journal Article] Minor contribution of an internal ribosome entry site in the 5'-UTR of ornithine decarboxylase mRNA on its translation2007
Author(s)
Nishimura, K., Sakuma, A., Yamashita, T., Hirokawa, G., Imataka, H., Kashiwagi, K., Igarashi, K.
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Journal Title
Biochem.Biophys.Res.Commun. 364
Pages: 124-130
Related Report
Peer Reviewed
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[Presentation] Binding of spermine and ifenprodil to a regulatory domain (R-domain) of the N-methyl-D-aspartate (NMDA) receptor and the role of R-domain on the activity of NMDA receptor2009
Author(s)
Kashiwagi, K., Han, X., Mizuno, S., Tomitori, H., Higashi, K., Terui, Y., Nishimura, K., Toida, T., Williams, K., Igarashi, K.
Organizer
第31回生体膜と薬物の相互作用シンポジウム
Place of Presentation
大阪大学コンベンションセンター場
Year and Date
2009-12-01
Related Report
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[Presentation] Binding of spermine and ifenprodil to a regulatory domain (R-domain) of the N-methyl-D-aspartate (NMDA) receptor and the role of R-domain on the activity of NMDA receptor.2009
Author(s)
Kashiwagi, K., Han, X., Mizuno, S., Tomitori, H., Higashi, K., Terui, Y., Nishimura, K., Toida, T., Williams, K., Igarashi, K.
Organizer
第31回生体膜と薬物の相互作用シンポジウム
Place of Presentation
大阪大学コンベンションセンター
Year and Date
2009-12-01
Related Report
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[Presentation] Isolation and characterization of an acrolein tolerant mutant of FM3A cells.2009
Author(s)
Tomitori, H., Nakamura, M., Yoshida, M., Kashiwagi, K., Igarashi, K.
Organizer
Gordon Research Conference on Polyamines
Place of Presentation
Waterville Valley, NH, USA
Year and Date
2009-06-24
Related Report
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[Presentation] Intense correlation between brain infarction and protein-conjugated acrolein.2009
Author(s)
Saiki, R., Nishimura, K., Ishii, I., Omura, T., Okuyama, S., Kashiwagi, K., Igarashi, K.
Organizer
Gordon Research Conference on Polyamines
Place of Presentation
Waterville Valley, NH, USA
Year and Date
2009-06-24
Related Report
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[Presentation] Polyamine effect on protein synthesis and its mechanism2009
Author(s)
Kashiwagi, K., Terui, Y., Higashi, K., Okudaira, H., Ochiai, E., Tomitori, H., Nishimura, K., Yamamoto, K., Ishihama, A., Igarashi, K.
Organizer
Gordon Research Conference on Polyamines
Place of Presentation
Waterville Valley, NH, USA
Year and Date
2009-06-22
Related Report
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[Presentation] Polyamine effect on protein synthesis and its mechanism.2009
Author(s)
Kashiwagi, K., Terui, Y., Higashi, K., Okudaira, H., Ochiai, E., Tomitori, H., Nishimura, K., Yamamoto, K., Ishihama, A., Igarashi, K.
Organizer
Gordon Research Conference on Polyamines
Place of Presentation
Waterville Valley, NH, USA
Year and Date
2009-06-22
Related Report
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[Presentation] Identification and function of amino acid residues on PotB and PotC involved in spermidine uptake activity.2009
Author(s)
Higashi, K., Sakamaki, Y., Uemura, T., Herai, E., Demizu, R., Zenda, R., Toida, T., Kashiwagi, K., Igarashi, K.
Organizer
Gordon Research Conference on Polyamines
Place of Presentation
Waterville Valley, NH, USA
Year and Date
2009-06-22
Related Report
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[Presentation] Polyamine stimulation of the synthesis of RpoE and StpA at the level of translatiohn in Escherichia coli under heat shock conditions.2009
Author(s)
Terui, Y., Tabei, Y., Higashi, K., Tomitori, H., Yamamoto, K., Ishihama, A., Igarashi, K., Kashiwagi, K.
Organizer
Biogenic Amines : Biochemical, Physiological, and Clinical Aspects
Place of Presentation
Bertinoro, Italy
Year and Date
2009-05-14
Related Report
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[Presentation] アフリカツメガエル胚発生に対するポリアミンの作用2008
Author(s)
塩川光一郎,近藤剛士,麻生舞,高井惇一,田久保繁樹,見村朋美,高橋宜治,梶谷正行,照井裕介,五十嵐一衛
Organizer
第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
Place of Presentation
神戸ボートアイランド
Year and Date
2008-12-12
Related Report
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[Presentation] 大腸菌スペルミジン優先取り込み系の膜貫通蛋白質PodB及びPotCの性質2008
Author(s)
出水梨沙,東恭平,酒巻善春,植村武史,戸来江美子,善田理沙,西村和彦,柏木敬子,五十嵐一衛,木敬子,五十嵐一衛
Organizer
第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
Place of Presentation
神戸ポートアイランド
Year and Date
2008-12-09
Related Report
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[Presentation] NMDA受容体の膜領域の構造の解析2008
Author(s)
金麗花, 田村真紀, 瀧川美紀, 廣瀬直雄, 西村和洋, 戸井田敏彦, K. Williams, 柏木敬子, 五十嵐一衛
Organizer
日本薬学会第128年会
Place of Presentation
パシフィコ横浜
Year and Date
2008-03-28
Related Report
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[Presentation] Acrolein toxicity : its mechanism and comparison of toxicity with reactive oxygen species2007
Author(s)
Yoshida, M., Hagihara, M., Tomitori, H., Nishimura, K., Kashiwagi, K., Igarashi, K.
Organizer
International Congress on Biogenic Amines : Biological and Clinical Perspectives
Place of Presentation
Sicily, Italy
Year and Date
2007-10-17
Related Report
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