Mechanisms of transcriptional regulation by histone H2A specific deubiquitylase

• Project/Area Number: 19390077
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Nagasaki University
• Principal Investigator: ITO Takashi Nagasaki University, 大学院・医歯薬学総合研究科, 教授 (90306275)
• Co-Investigator(Kenkyū-buntansha): NAKAGAWA Takeya 長崎大学, 大学院・医歯薬学総合研究科, 助教 (50363502) ; NANBA Yasuaki 長崎大学, 大学院・医歯薬学総合研究科, 助教 (90444869)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2007: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
• Keywords: クロマチン / 遺伝子転写 / ヒストン / 翻訳後修飾 / ユビキチン化 / H2A / 部分肝切除 / 肝再生 / 転写開始 / 高次構造 / マイクロアレイ / USP21 / 脱ユビキチン化

Research Abstract

真核細胞は遺伝情報を安定に保ち、正確に発現し、細胞分化を維持するため、多彩な機構を働かせている。H2Aの118番目のリジン残基はユビキチン化されることが30年前に明らかにされている。われわれは今回の研究により、このH2A118Kのユビキチン化がH3K4 のメチル化とクロストークすることにより遺伝子転写開始を調節していることを明らかにした

Research Products

• [Journal Article] 肝臓再生とユビキチン化ヒストンH2Aによる遺伝子転写制御 (2008)
  • Author(s): 伊藤 敬
  • Journal Title: 実験医学 26 Pages: 1347-1352
• [Journal Article] Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di-and trimethylation. (2008)
  • Author(s): Nakagawa, T., Kajitani, T., Togo, S., Masuko, N., Ohdan, H., Hishlkawa, Y., Koji, T., Matsuyama, T., Ikura, T., Murainatsu, M., and Ito, T.
  • Journal Title: Genes Dev 22 Pages: 37-49
  • Peer Reviewed
• [Journal Article] (2007). Role of histone phosphorylation in chromatin dynamics and its implications in diseases (2007)
  • Author(s): Oki, M., Aihara, H., and Ito, T.
  • Journal Title: Subcell Biochem 41 Pages: 319-336
• [Journal Article] Role of histone modification in chromatin dynamics. (2007)
  • Author(s): Ito, T.
  • Journal Title: J Biochem(Tokyo) 141 Pages: 609-614
  • NAID: 10020098988
  • Peer Reviewed
• [Journal Article] Concerted action of Aurora B,Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation. (2007)
  • Author(s): Brittle, A.L., Nanba, Y., Ito, T., and Ohkura, H.
  • Journal Title: Exp Cell Res 313 Pages: 2780-2785
  • Peer Reviewed
• [Journal Article] 2007. DNA damage-dependent acetylation and ubiquitination of H2AX enhances chromatin dynamics (2006)
  • Author(s): Ikura, T., Tashiro, S., Kakino, A., Shima, H., Jacob, N., Amunugama, R., Yoder, K., Izumi, S., Kuraoka, I., Tanaka, K., Kimura, H., Ikura, M., Nishikubo, S., Ito, T., Muto, A., Miyagawa, K., Takeda, S., Fishel, R., Igarashi, K., and Kamiya, K.
  • Journal Title: Mol Cell Biol 27(20) Pages: 7028-7040
  • Peer Reviewed
• [Journal Article] 2007. Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation (2006)
  • Author(s): Brittle, A.L., Nanba, Y., Ito, T., and Ohkura, H.
  • Journal Title: Exp Cell Res. 313(13) Pages: 2780-2785
  • Peer Reviewed
• [Journal Article] 2008. Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di- and trimethylation (2005)
  • Author(s): Nakagawa, T., Kajitani, T., Togo, S., Masuko, N., Ohdan, H., Hishikawa, Y., Koji, T., Matsuyama, T., Ikura, T., Muramatsu, M., and Ito, T
  • Journal Title: Genes Dev Dev22(1) Pages: 37-49
  • Peer Reviewed
• [Journal Article] 2007. Role of histone modification in chromatin dynamics (2005)
  • Author(s): Ito, T
  • Journal Title: J Biochem (Tokyo) 141(5) Pages: 609-614
  • Peer Reviewed
• [Presentation] Histone modification and Transcriptional Regulation Takashi Ito, Department of Biochemistry, Nagasaki University of Medicine (2008)
  • Author(s): Takashi Ito
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] 細胞核ダイナミックスとRNA(1)内藤カンファレンス(Histone modification and Transcriptional Regulation)(Oral presentation)(Poster Session) (2008)
  • Author(s): Chair Takashi Ito & William Lee Kraus
  • Organizer: Session C : Chromatin structure and Histone Modifications during Transcription
• [Presentation] Deubiquitylation of hisotne H2A activates transcriptiona initiation via trans-histone crosstalk with H3K4 di-and tri-methylation. (2008)
  • Author(s): Nakagawa, T., Takuya Kajitani, Shinji Togo, Norio Masuko, Yoshitaka Hishikawa, Koji Takehiko, Toshifumi Matsuyama, Hideki, Ohdan, Tsuyoshi Ikura, Masami Muramatsu and Takashi Ito
  • Organizer: Keystone Symposia
  • Place of Presentation: Keystone
• [Presentation] Role of histone phosphorylation in chromatin dynamics
  • Author(s): Hirofumi Mizusaki, Hitoshi Aihara, Haruhiko Koseki, Masami Muramatsu, Takashi Ito
  • Place of Presentation: Department of Biochemistry, Nagasaki University School of Medicine(Department of Biochemistry, Nagasaki University School of MedicineDepartment of Biochemistry, Nagasaki University School of MedicineDepartment of Biochemistry, Nagasaki University School
• [Presentation] ATP-Dependent Stimulator of Nucleosomal Histone Acetylation (ASNA) modifies H2A during Transcriptional Activation
  • Author(s): Masamichi Doiguchi, Takeya Nakagawa, Hitoshi Ueda, Masami Muramatsu, and Takashi Ito
  • Place of Presentation: Department of Biochemistry, Nagasaki University of Medicine, Nagasaki, Japan1. The Graduate School of Natural Science and Technology and Department of Biology, Faculty of Science, Okayama University, Tsushima-naka, Okayama, Japan2. Saitama Medical School
• [Presentation] Keystone Symposia 2008 Regulatory Mechanismas in Eukaryotic transcription(Poster Session 2)
  • Author(s): Takeya Nakagawa, Takuya Kajitani, Shinji Togo, Norio Masuko, Hideki, Ohdan, Yoshitaka Hishikawa, Koji Takehiko, Toshifumi Matsuyama, Tsuyoshi Ikura, Masami Muramatsu and Takashi Ito,
  • Organizer: Deubiquitylation of hisotne H2A activates transcriptiona initiation via trans-histone crosstalk with H3K4 di- and tri-methylation.Kevin Struhl, and John T. Lis
  • Place of Presentation: Keystone Ressort Keystone, Colorado
• [Book] 分子生物学イラストレイテッド改訂第3版 (2008)
  • Author(s): 伊藤 敬(分担執筆)
  • Publisher: 羊土社