Identification of erythrocyte proteins interacting with RhopH complex of malaria parasites

• Project/Area Number: 19390120
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Ehime University
• Principal Investigator: TORII Motomi Ehime University, 大学院・医学系研究科, 教授 (20164072)
• Co-Investigator(Kenkyū-buntansha): OTSUKI Hitoshi 愛媛大学, 大学院・医学系研究科, 助教 (80403806)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2008: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2007: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
• Keywords: 原虫 / マラリア / 感染症 / マラリア原虫 / 赤血球接着 / レセプター

Research Abstract

マラリア原虫メロゾイトは、宿主赤血球侵入時に先端部小器官からタンパク質を分泌する。先端部小器官から分泌されるタンパク質の中で、ロプトリーから分泌される高分子ロプトリータンパク質複合体(RhopH複合体)の赤血球侵入における役割は分かっていない。本研究では、RhopHの赤血球侵入時における機能の解明に向けて、RhopH複合体と相互作用する赤血球タンパク質の検索を行った結果、RhopH複合体と結合するタンパク質を見いだすことに成功した。

Research Products

• [Journal Article] Single amino acid substitution in Plasmodium yoelii erythrocyte ligand determines its localization and controls parasite virulence (2009)
  • Author(s): Otsuki H, Kaneko O, Thongkukiatkul A, Tachibana M, Iriko H, Takeo S, Tsuboi T, Torii M.
  • Journal Title: Proc Natl Acad Sci (in press)(電子版)
  • NAID: 120006984096
  • Peer Reviewed
• [Journal Article] Single amino acid substitution in Plasmodiun yoelii erythrocyte ligand determinesits localization and controls parasite virulence. (2009)
  • Author(s): Otsuki H, Kaneko O, Thongkukiatkul. A, Tachibana M, Iriko H, Takeo S, Tsuboi T, Torii M
  • Journal Title: Rroc Natl Acad Sci USA (in press)
  • Peer Reviewed
• [Journal Article] The Plasmodium falciparum RhopH2 promoter and first 24 amino acids are sufficient to target proteins to the rhoptries (2007)
  • Author(s): Ghoneim A, Kaneko O, Tsuboi T, Torii M.
  • Journal Title: Parasitol Int 56 Pages: 31-43
  • NAID: 10018566061
  • Peer Reviewed
• [Presentation] A complex formation of rhoptry neck protein 2 with a microneme protein, AMA1, in Plasmodium falciparum (2008)
  • Author(s): Cao J, Kaneko O, Thongkukiatkul A, Tachibana M, Otsuki H, Tsuboi T, Torii M.
  • Organizer: 57^<th> ASTMH Annual Meeting, New Orleans
  • Place of Presentation: USA
  • Year and Date: 2008-07-11
• [Presentation] Erythrocyte-Binding-Like molecule and Virulence of Plasmodium yoelii (2008)
  • Author(s): Otsuki H, Kaneko O, Thongkukiatkul A, Tachibana M, Iriko H, Takeo S, Tsuboi T, Torii M.
  • Organizer: 19th Annual Molecular Parasitology Meeting
  • Place of Presentation: Woods Hole, USA
• [Presentation] ネズミマラリア原虫の赤血球結合分子相同体EBLの局在と病原性 (2008)
  • Author(s): 大槻均、金子修、入子英幸、竹尾暁、坪井敬文、Thongkukiatkul Amporn、鳥居本美
  • Organizer: 第77回日本寄生虫学会大会
  • Place of Presentation: 長崎市
• [Presentation] A complex formation of rhoptry neck protein 2 with a microneme protein, AMA1, in Plasmodium falciparum (2008)
  • Author(s): 曹俊、金子修、Thongkukiatkul Amporn、橘真由美、大槻均、坪井敬文、鳥居本美
  • Organizer: 第77回日本寄生虫学会大会
  • Place of Presentation: 長崎市
• [Presentation] A complex formation of rhoptry neck protein 2 with a microneme protein, AMA1, in Plasmodium falciparuum. (2008)
  • Author(s): Cao J, Kaneko O, Thongkukiatkul A, Tachibana M, Otsuki H, Tsuboi T, Torii M
  • Organizer: 57^<th> ASTMH Annual Meeting
  • Place of Presentation: New Orleans, USA
• [Presentation] Erythrocyte-Binding-Like molecule and Virulence of Plasmodiun yoelii. (2008)
  • Author(s): Otsuki H, Kaneko O, Thongkukiatkul A, Tachibana M, Iriko H, Takeo S, TsuboiT, Torii M
  • Organizer: 19th Annual Molecular Parasitology Meeting
  • Place of Presentation: Woods Hole, USA
• [Presentation] A ccmplex formation of rhoptry neck protein 2 with a microneme protein, AMA1, in Plasmodium falciparum. (2008)
  • Author(s): 曹俊, 金子修, Thongkukiatkul Amporn, 橘真由美, 大槻均, 坪井敬文, 鳥居本美
  • Organizer: 第77回日本寄生虫学会大会
  • Place of Presentation: 長崎市
• [Presentation] A complex formation of rhoptry neck protein 2 with a microneme protein, AMA1, in Plesmodium falciparum (2007)
  • Author(s): Cao J, Kaneko O, Thongkukiatkul A, Tachibana M, Otsuki H, Tsuboi T, Torii M.
  • Organizer: 第6回分子寄生虫・マラリア研究フォーラム
  • Place of Presentation: 松山
• [Presentation] Plasmodium falciparum rhoptry neck protein (PfRON2) expressed at both erythrocytic and pre-erythrocytic invasive parasites (2007)
  • Author(s): Cao J, Kaneko O, Thongkukiatkul A, Tachibana M , Otsuki H, Sattabongkot J, Tsuboi T, Torii M.
  • Organizer: The 7^<th> Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji