Induction of Bone Regeneration by Controlled Release of Osteogenesis/Angiogenesis Factors from Titanium Implants

• Project/Area Number: 19390494
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 補綴理工系歯学
• Research Institution: Osaka University
• Principal Investigator: YATANI Hirofumi Osaka University, 大学院・歯学研究科, 教授 (80174530)
• Co-Investigator(Kenkyū-buntansha): EGUSA Hiroshi 大阪大学, 大学院・歯学研究科, 助教 (30379078) ; TABATA Yasuhiko 京都大学, 再生医科学研究所, 教授 (50211371)
• Co-Investigator(Renkei-kenkyūsha): TABATA Yasuhiko 京都大学, 再生医科学研究所, 教授 (50211371)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2008: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2007: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
• Keywords: インプラント / 骨再生 / 薬剤徐放 / 小分子化合物 / ペプチド / 骨芽細胞 / 破骨細胞 / b-FGF

Research Abstract

The aim of this study is to establish a novel technology to achieve efficient bone regeneration by controlled release of bioactive factors from titanium implants. We designed a titanium implant chamber which contains a biodegradable material releasing bioactive factor (b-FGF). We found that this system induces bone regeneration around the implant in an animal model. We also identified osteogenesis inducible peptide and small molecule, and elucidated mechanisms of these molecules on bone regeneration.

Research Products

• [Journal Article] A small-molecule approach to bone regenerative medicine in dentistry (2010)
  • Author(s): Egusa H, Saeki M, Doi M, Fukuyasu S, Matsumoto T, Kamisaki Y, Yatani H
  • Journal Title: Journal of Oral Biosciences Volume: 52
  • NAID: 10027105562
  • Description: 印刷中
  • Peer Reviewed
• [Journal Article] Enhanced bone regeneration via multimodal actions of synthetic peptide SVVYGLR on osteoprogenitors and osteoclasts. (2009)
  • Author(s): Egusa H, Kaneda Y, Akashi Y, Hamada Y, Matsumoto T, Saeki M, Thakor DK, Tabata Y, Matsuura N, Yatani H
  • Journal Title: Biomaterials 30 Pages: 4676-4686
  • Peer Reviewed
• [Journal Article] Enhanced bone regeneration Via multimodal actions of synthetic peptide SVVYGLR on osteoprogenitors and osteoclasts (2009)
  • Author(s): Egusa H, Kaneda Y, Akashi Y, Hamada Y, Matsumoto T, Sacki M, Thakor Dk, Tabata Y, Matsuura N, Yatani H
  • Journal Title: Biomaterals 30 Pages: 4676-4686
  • Peer Reviewed
• [Journal Article] Enhanced bone regeneration via multimodal actions of synthetic peptide SVVYGLR on osteoprogenitors and osteoclasts (2009)
  • Author(s): Egusa H, Kaneda Y, Akashi Y, Hamada Y, Matsumoto T, Saeki M, Thakor Dk, Tabata Y, Matsuura N, Yatani H
  • Journal Title: Biomaterials Volume: 30 Pages: 4676-4686
  • Peer Reviewed
• [Journal Article] Synthetic osteopontin-derived peptide SVVYGLR can induce neovascularization in artificial bone marrow scaffold biomaterials. (2007)
  • Author(s): Hamada Y, Egusa H, Kaneda Y, Hirata I, Kawaguchi N, Hirao T, Matsumoto T, Yao M, Daito K, Suzuki M, Yatani H, Daito M, Okazaki M, Matsuura N
  • Journal Title: Dental Materials Journal 26 Pages: 487-492
  • NAID: 110006368707
  • Peer Reviewed
• [Journal Article] Synthetic osteopontin-derived peptide svvyglr can induce neovascularization in artificial bone marrow scaffold biomaterials (2007)
  • Author(s): Hamada Y, Egusa H, Kaneda Y, Hirata I, Kawaguchi N, Hirao T, Matsumoto T, Yao M, Daito K, Suzuki M, Yatani H, Daito M, Okazaki M, Matsuura N
  • Journal Title: Dental Materials Journal 26(4) Pages: 487-492
  • NAID: 110006368707
  • Peer Reviewed
• [Journal Article] Modification of apatite materials for bone tissue engineering and drug delivery carriers (2007)
  • Author(s): Matsumoto T., Okazaki M., Nakahira A., Sasaki J., Egusa H., Sohmura T.
  • Journal Title: Current Medicinal Chemistry 14(25) Pages: 2726-2733
  • Peer Reviewed
• [Journal Article] Synthetic osteopontin-derived peptide SVVYGLR can induce neovascularization in artificial bone marrow scaffold (2007)
  • Author(s): Hamada Y, Egusa H, Kaneda Y, Hirata I, Kawaguchi N, Hirao T, Matsumoto T, Yao M, Daito K, Suzuki M, Yatani H, Daito M, Okazaki M, Matsuura N
  • Journal Title: Dental Materials Journal Volume: 26 Pages: 487-492
  • Peer Reviewed
• [Journal Article] A small-molecule approach to bone regenerative medicine in dentistry.
  • Author(s): Egusa H, Saeki M, Doi M, Fukuyasu S, Matsumoto T, Kamisaki Y, Yatani H
  • Journal Title: Journal of Oral Biosciences (accepted)
  • NAID: 10027105562
  • Peer Reviewed
• [Journal Article] A small-molecule approach to bone regenerative medicine in dentistry
  • Author(s): Egusa H, Sacki M, Doi M, Fukuyasu S, Matsumoto T, Kamisaki Y.Yatani H
  • Journal Title: Journal of Oral Biosciences (in press)
  • NAID: 10027105562
  • Peer Reviewed
• [Presentation] Chemical library screening of orphan ligands for identifying osteoclastogenesis-related small molecular compounds (2009)
  • Author(s): Egusa H, Doi M, Saeki M, Kamisaki Y, Yatani H
  • Organizer: 26th Naito Conference on Osteobiology
  • Place of Presentation: 兵庫
  • Year and Date: 2009-11-04
• [Presentation] Chemical library screening of orphan ligands for identifying osteoclastogenesis-related small molecular compounds (2009)
  • Author(s): Egusa H, Doi M, Saeki M, Kamisaki Y, Yatani H
  • Organizer: 26th Naito Conferece on Osteobiology
  • Place of Presentation: 兵庫
  • Year and Date: 2009-11-04
• [Presentation] Dental Implant Treatment & Regenerative Medicine-The Impact of Biotechnology on Current and Future Oral Implantology- (2009)
  • Author(s): Egusa H
  • Organizer: Oral Health & Science Seminar in the University of Hong Kong, Faculty of Dentistry
  • Place of Presentation: 香港, 中国.
  • Year and Date: 2009-10-28
• [Presentation] Dental Implant Treatment & Regenerative Medicine-The Impact of Biotechnology on Current and Future Oral Implantology- (2009)
  • Author(s): Hiroshi Egusa
  • Organizer: Oral Health & Scienee Seminar in the University of Hong Kong, Faculty of Dentistry
  • Place of Presentation: 香港,中国
  • Year and Date: 2009-10-28
• [Presentation] Dental Implant Treatment & Regenerative Medicine -The Impact of Biotechnology on Current and Future Oral Implantology- (2009)
  • Author(s): Hiroshi Egusa
  • Organizer: Oral Health & Science Seminar in the University of Hong Kong, Faculty of Dentistry
  • Place of Presentation: 香港, 中国
  • Year and Date: 2009-10-28
  • Invited
• [Presentation] 合成ペプチドSVVYGLRの骨組織再生作用 (2009)
  • Author(s): 江草宏, 金田善俊, 萱島浩輝, 福安翔, 明石喜裕, 矢谷博文
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟.
  • Year and Date: 2009-09-11
• [Presentation] 合成ペプチトSVVYGLRの骨組織再生作用 (2009)
  • Author(s): 江草宏, 金田善俊, 萓島浩輝, 福安翔, 明石喜裕, 矢谷博文
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-11
• [Presentation] 合成ペプチドSVVYGLRの骨組織再生作用 (2009)
  • Author(s): 江草 宏, 金田善俊, 萱島浩輝, 福安 翔, 明石喜裕, 矢谷博文
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-11
• [Presentation] 小分子化合物harmineは破骨前駆細胞におけるNFATc1およびId2発現を増強する (2009)
  • Author(s): 江草宏, 土居正典, 佐伯万騎男, 矢谷博文
  • Organizer: 第27回日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-23
• [Presentation] 小分子化合物harmineは破骨前駆細胞におけるNFATelおよびld2発現を増強する (2009)
  • Author(s): 江草宏, 土居正典, 佐伯万騎男, 矢谷博文
  • Organizer: 第27回日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-23
• [Presentation] 小分子化合物harmineは破骨前駆細胞におけるNFATclおよびId2発現を増強する (2009)
  • Author(s): 江草 宏, 土居正典, 佐伯万騎男, 矢谷博文
  • Organizer: 第27回日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-23
• [Presentation] 小分子化合物harmineは破骨前駆細胞におけるNFATc1およびId2発現を増強する (2009)
  • Author(s): 土居正典, 江草宏, 佐伯万騎男, 矢谷博文, 上崎善規
  • Organizer: 第108回大阪大学歯学会例会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-16
• [Presentation] 小分子化合物harmineは破骨前駆細胞におけるNFATclおよびld2発現を増強する (2009)
  • Author(s): 土居正典, 江草宏, 佐伯万騎男, 矢谷博文, 上崎善規
  • Organizer: 第108回大阪大学歯学会例会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-16
• [Presentation] 小分子化合物harmineは破骨前駆細胞におけるNFATclおよびId2発現を増強する (2009)
  • Author(s): 土居正典, 江草 宏, 佐伯万騎男, 矢谷博文, 上﨑善規
  • Organizer: 第108回大阪大学歯学会例会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-16
• [Presentation] 再生医学とインプラント治療-近未来のインプラント学におけるバイオテクノロジーのインパクト- (2009)
  • Author(s): 江草宏
  • Organizer: 第118回日本補綴歯科学会シンポジウム「インプラントと再生医療」
  • Place of Presentation: 京都.
  • Year and Date: 2009-06-06
• [Presentation] 再生医学とインプラント治療-近未来のインプラント学におけるバイオテクノロジーのインパクト- (2009)
  • Author(s): 江草宏
  • Organizer: 第118回日本補綴歯科学会シンポジウム「インプラントと再生医療」
  • Place of Presentation: 京都
  • Year and Date: 2009-06-06
• [Presentation] 再生医学とインプラント治療-近未来のインプラント学におけるバイオテクノロジーのインパクト- (2009)
  • Author(s): 江草 宏
  • Organizer: 第118回日本補綴歯科学会 シンポジウム「インプラントと再生医療」
  • Place of Presentation: 京都
  • Year and Date: 2009-06-06
  • Invited
• [Presentation] Enhanced Bone Regeneration with Bioactive Synthetic Peptide SVVYGLR. (2009)
  • Author(s): Egusa H, Kaneda Y, Akashi Y, Kayashima H, Yatani H
  • Organizer: 6th Biennial Congress of Asian Academy of Prosthodontics
  • Place of Presentation: Seoul, Korea.
  • Year and Date: 2009-04-25
• [Presentation] Enhanced Bone Regeneration with Bioactive Synthetic Peptide SVVYGLR (2009)
  • Author(s): Egusa H, Kaneda Y, Akashi Y, Kayashima H, Yatani H
  • Organizer: 6th Biennial Congress of Asian Academy of Prosthodontics
  • Place of Presentation: Seoul,韓国
  • Year and Date: 2009-04-24
• [Presentation] Enhanced Bone Regeneration with Bioactive Synthetic Peptide SVVYGLR (2009)
  • Author(s): Egusa H, Kaneda Y, Akashi Y, Kayashima H, Yatani H
  • Organizer: 6th Biennial Congress of Asian Academy of Prosthodontics
  • Place of Presentation: Seoul, 韓国
  • Year and Date: 2009-04-24
• [Presentation] Development of high-throughput screening system for identifying osteoclastogenesis related compounds. (2009)
  • Author(s): Egusa H, Doi M, Saeki M, Kamisaki Y, Yatani H
  • Organizer: 87th IADR General Session
  • Place of Presentation: Miami, USA.
  • Year and Date: 2009-04-03
• [Presentation] Development of high-throughput screening system for identifying osteoclastogenesis-related compounds (2009)
  • Author(s): Egusa H, Doi M, Saeki M, Kamisaki Y, Yatani H
  • Organizer: 87th IADR(国際歯学研究学会)General Session
  • Place of Presentation: Miami, USA
  • Year and Date: 2009-04-03
• [Presentation] Bone regeneration induced by functional peptide SVVYGLR. (2009)
  • Author(s): Kaneda Y, Egusa H, Akashi Y, Ashida S, Hamada Y, Yatani H
  • Organizer: 87th IADR General Session
  • Place of Presentation: Miami, USA.
  • Year and Date: 2009-04-01
• [Presentation] Bone regeneration induced by functional peptide SVVYGLR (2009)
  • Author(s): kaneda Y, Egusa H, Akashi Y, Ashida S, Hamada Y, Yatani H
  • Organizer: 87th IADR(国際歯学研究学会)General Session
  • Place of Presentation: Miami, USA
  • Year and Date: 2009-04-01
• [Presentation] HarmineのNFAT発現増強作用 (2008)
  • Author(s): 土居正典, 江草宏, 佐伯万騎男, 飯田務, 上崎善規, 矢谷博文
  • Organizer: 第50回歯科基礎医学会学術大会
  • Place of Presentation: 東京.
  • Year and Date: 2008-09-25
• [Presentation] HarmineのNFAT発現増強作用 (2008)
  • Author(s): 土居正典, 江草 宏, 佐伯万騎男, 飯田 務, 上﨑善規, 矢谷博文
  • Organizer: 第50回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-25
• [Presentation] オーファンリガンドライブラリーを用いた破骨細胞分化関連化合物のスクリーニング (2008)
  • Author(s): 江草宏
  • Organizer: 第50回歯科基礎医学会学術大会シンポジウム「硬組織の明るい未来を目指して」シンポジスト
  • Place of Presentation: 東京.
  • Year and Date: 2008-09-23
• [Presentation] オーファンリガンドライブラリーを用いた破骨細胞分化関連化合物のスクリーニング (2008)
  • Author(s): 江草 宏
  • Organizer: 第50回歯科基礎医学会学術大会シンポジウム「硬組織の明るい未来を目指して」
  • Place of Presentation: 東京
  • Year and Date: 2008-09-23
  • Invited
• [Presentation] DNA methylation analysis of osteogenic gene promoter regions in mouse bone marrow stromal cells (2008)
  • Author(s): Egusa H, Ashida S, Kobayashi M, Akashi Y, Yatani H
  • Organizer: American Society for Bone and Mineral Research Annual Meeting
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2008-09-15
• [Presentation] Global methylation screening in mesenchymal stem cell genomes during osteogenesis (2008)
  • Author(s): Egusa H, Ashida S, Kobayashi M, Yatani H, Nishimural
  • Organizer: 86^<th> IADR (国際歯科学会)General Session
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-04
• [Presentation] 機能性ペプチドSVVYGLRを用いた骨組織再生の試み (2008)
  • Author(s): 金田善俊, 江草宏, 濱田吉之輔, 矢谷博文
  • Organizer: 第117回日本補綴歯科学会学術大会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-06-07
• [Presentation] 機能性ペプチドSVVYGLRが歯周組織細胞へ及ぼす影響 (2007)
  • Author(s): 金田善俊, 江草宏, 濱田吉之輔, 松浦成昭, 矢谷博文
  • Organizer: 第104回大阪大学歯学会例会
  • Place of Presentation: 大阪.
  • Year and Date: 2007-07-19
• [Presentation] 機能性ペプチドSVVYGLRが歯周組織細胞へ及ぼす影響 (2007)
  • Author(s): 金田善俊, 江草 宏, 濱田吉之輔, 松浦成昭, 矢谷博文
  • Organizer: 第104回大阪大学歯学会例会
  • Place of Presentation: 大阪
  • Year and Date: 2007-07-19
• [Presentation] Effects of osteopontin-derived peptide SVVYGLR on cells from mesenchymal tissues. (2007)
  • Author(s): Kaneda Y, Egusa H, Hamada Y, Ashida S, Kobayashi M, Yatani H
  • Organizer: 85th IADR General Session
  • Place of Presentation: New Orleans, USA.
  • Year and Date: 2007-03-21
• [Presentation] Effects of osteopontin-derived peptide SVVYGLR on cells from mesenchymal tissues (2007)
  • Author(s): Kaneda Y, Egusa H, Hamada Y, Ashida S, Kobayashi M, Yatani H
  • Organizer: 85^<th> IADR General Session
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2007-03-21
• [Presentation] Effects of osteopontin-derived peptide SVVYGLR on cells from mesenchymal tissues (2007)
  • Author(s): Kaneda Y, Egusa H, Hamada Y, Ashida S, Kobayashi M, Yatani H
  • Organizer: 85th International Association for Dental Research (IADR) General Session
  • Place of Presentation: New Orleans, USA
• [Book] 顎口腔機能の再建をめざして「生命歯科医学のカッティング・エッジ」(米田俊之編) (2008)
  • Author(s): 矢谷博文,中村隆志,瑞森崇弘,石垣尚一,山田真一,江草宏
  • Publisher: 大阪大学出版会
• [Book] 顎口腔機能の再建をめざして「生命歯科医学のカッティング・エッジ」 (2008)
  • Author(s): 矢谷博文, 中村隆志, 瑞森崇弘, 石垣尚一, 山田真一, 江草 宏
  • Publisher: 大阪大学出版会
• [Remarks] 江草宏:日本補綴歯科学会特定推進研究優秀論文賞受賞,日本補綴歯科学会,平成22年6月13日受賞式.
• [Remarks] 江草宏:第27回日本骨代謝学会優 秀ポスター演題賞受賞,日本骨代謝学会:平成21年7月23日.
• [Remarks] Egusa H:第6回アジア補綴歯科学会最 優秀ポスター発表賞,Asian Academy of Prosthodontics:平成21年4月26日.
• [Remarks] Kaneda Y, Egusa H, Yatani H, et al.: Arthur R. Frechette Prosthodontic Research Award Finalist,国際歯科研究学会:平成21年4月3日.