Exploration of novel cold-adapted microorganisms to develop a systemfor the production of useful compounds at low temperatures
| Project/Area Number |
19404020
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kyoto University |
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Principal Investigator |
KURIHARA Tatsuo Kyoto University, 化学研究所, 准教授 (70243087)
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| Co-Investigator(Kenkyū-buntansha) |
ESAKI Nobuyoshi 京都大学, 化学研究所, 教授 (50135597)
MIHARA Hisaaki 京都大学, 化学研究所, 助教 (30324693)
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| Project Period (FY) |
2007 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2007: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Keywords | 低温適応微生物 / 低温菌 / 好冷菌 / 低温物質生産 / 低温誘導性遺伝子 / タンパク質分泌生産 |
|---|
| Research Abstract |
アラスカ永久凍土やスイス高山土壌から低温での生育特性やタンパク質の分泌生産特性に優れた細菌を分離した。アラスカ永久凍土由来の低温適応微生物がヘモリシン共制御タンパク質のホモログを分泌高生産することを見いだし、本タンパク質の分泌装置を利用した外来タンパク質分泌生産系構築に向けて分泌機構を解析した。一方、南極海水から分離した低温菌について、本菌を宿主としたタンパク質生産系構築において利用価値が高いと考えられる低温誘導性プロモーターを単離した。
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium2009
Author(s)
Kawamoto, J., Kurihara, T., Yamamoto, K., Nagayasu, M., Tani, Y., Mihara, H., Hosokawa, M., Baba, T., Sato, S. B. and Esaki, N.
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Journal Title
Shewanella livingstonensis Ac10. J. Bacteriol 191
Pages: 632-40
Related Report
Peer Reviewed
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[Journal Article] Cold adaptation of eicosapentaenoic acid-less mutant of Shewanella livingstonensis Ac10 involving uptake and remodeling of synthetic phospholipids containing various polyunsaturated fatty acids2008
Author(s)
Sato, S., Kurihara, T., Kawamoto, J., Hosokawa, M., Sato, S. B. and Esaki, N.
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Journal Title
Extremophiles 12
Pages: 753-61
Related Report
Peer Reviewed
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[Journal Article] Proteomic studies of an Antarctic cold-adapted bacterium, Shewanella livingstonensis Ac10, for global identification of cold-inducible proteins2007
Author(s)
Kawamoto, J., Kurihara, T., Kitagawa, M., Kato, I. and Esaki, N.
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Journal Title
Extremophiles 11
Pages: 819-26
Related Report
Peer Reviewed
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[Journal Article] Construction of a low-temperature protein expression system using a cold-adapted bacterium, Shewanella sp. strain Ac10, as the host2007
Author(s)
Miyake, R., Kawamoto, J., Wei, Y.-L., Kitagawa, M., Kato, I., Kurihara, T. and Esaki, N.
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Journal Title
Appl. Environ. Microbiol 73
Pages: 4849-56
Related Report
Peer Reviewed
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