Neuronal mechanisms for determination of basic respiratory cycle by dual rhythm generators in the medulla

• Project/Area Number: 19500277
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Showa University
• Principal Investigator: ONIMARU Hiroshi Showa University, 医学部・第二生理, 准教授 (30177258)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 呼吸リズム / 傍顔面呼吸ニューロン / 延髄 / 呼吸中枢 / 中枢化学受容 / Phox2b / 呼吸リズム形成 / 神経科学 / 脳・神経 / プレベッチンガー / 新生ラット / 摘出脳標本 / 顔面神経核

Research Abstract

Two distinct neuronal networks ; the pre-Botzinger complex (pre-BotC) and the parafacial respiratory group (pFRG), in the ventrolateral medulla (VLM) are involved in respiratory rhythm generation. In the present study, we have studied the neuronal mechanisms for determination of basic respiratory rhythm by interaction between these neuron groups. We found that the pFRG is necessary for postnatal survival because of its involvement in rhythm generation and central chemoreception in the neonatal system.

Research Products

• [Journal Article] Phox2b, RTN/pFRG neurons and respiratory rhythmogenesis. (2009)
  • Author(s): Onimaru H, Ikeda K, Kawakami K.
  • Journal Title: Respir Physiol Neurobiol. 168(1-2) Pages: 13-8
  • Peer Reviewed
• [Journal Article] Electrophysiological responses of sympathetic preganglionic neurons to ANG II and aldosterone. (2009)
  • Author(s): Minoura Y, Onimaru H, Iigaya K, Homma I, Kobayashi Y.
  • Journal Title: Am J Physiol Regul Integr Comp Physiol. 297
  • Peer Reviewed
• [Journal Article] Opioids prolong and anoxia shortens delay between onset of preinspiratory (pFRG) and inspiratory (preBotC) network bursting in newborn rat brainstems. (2009)
  • Author(s): Ballanyi K, Ruangkittisakul A, Onimaru H
  • Journal Title: Pflugers Arch. 458 Pages: 571-87
  • Peer Reviewed
• [Journal Article] Relation of Blood Pressure Quantitative Trait Locus on Rat Chromosome 1 to Hyperactivity of Rostralventrolateral Medulla. (2009)
  • Author(s): Iigaya K, Kumagai H, Nabika T, Harada Y, Onimaru H, Oshima N, Takimoto C, Kamayachi T, Saruta T, Itoh H.
  • Journal Title: Hypertension 53 Pages: 42-48
  • Peer Reviewed
• [Journal Article] Phox2b, RTN/pFRG neurons and respiratory rhythmogenesis (2009)
  • Author(s): Onimaru H, Ikeda K, Kawakami K.
  • Journal Title: Respir Physiol Neurobiol. 168 Pages: 13-18
  • Peer Reviewed
• [Journal Article] Electrophysiological responses of sympathetic preganglionic neurons to ANG II and aldosterone (2009)
  • Author(s): Minoura Y, Onimaru H, Iigaya K, Homma I, Kobayashi Y.
  • Journal Title: Am J Physiol Regul Integr Comp Physiol. 297
  • Peer Reviewed
• [Journal Article] Opioids prolong and anoxia shortens delay between onset of preinspiratory(pFRG)and inspiratory(preBotC)network bursting in newborn rat brainstems (2009)
  • Author(s): Ballanyi K, Ruangkittisakul A, Onimaru H.
  • Journal Title: Pflugers Arch. 458 Pages: 571-587
  • Peer Reviewed
• [Journal Article] Relation of Blood Pressure Quantitative Trait Locus on Rat Chromosome 1 to Hyperactivity of Rostralventrolateral Medulla. (2009)
  • Author(s): Iigaya K, Kumagai H, Onimaru H, 他
  • Journal Title: Hypertension 53 Pages: 42-48
  • Peer Reviewed
• [Journal Article] CO2-Sensitive Preinspiratory Neurons of the Parafacial Respiratory Group Express Phox2b in the Neonatal Rat. (2008)
  • Author(s): Onimaru H, Ikeda K, Kawakami K
  • Journal Title: J Neurosci. 28(48) Pages: 12845-12850
  • Peer Reviewed
• [Journal Article] Monosynaptic Excitatory Connection from the RostralVentrolateral Medulla to Sympathetic Preganglionic Neurons Revealed by Simultaneous Recordings To directly investigate whether a monosynaptic connection exists between neurons in the rostral ventrolateralM (2008)
  • Author(s): Oshima N, Kumagai H, Onimaru H, Kawai A, Pilowsky P M, Iigaya K, Takimoto C, Hayashi K, Saruta T, Itoh H.
  • Journal Title: Hypertens Res. 31 Pages: 1445-1454
  • Peer Reviewed
• [Journal Article] C02-Sensitive Preinspiratory Neurons of the Parafacial Respiratory Group Express Phox2b in the Neonatal Rat. (2008)
  • Author(s): Onimaru H, Ikeda K, Kawakami K
  • Journal Title: J Neurosci. 28 Pages: 12845-12850
  • Peer Reviewed
• [Journal Article] Monosynaptic Excitatory Connection from the RostralVentrolateral Medulla to Sympathetic Preganglionic Neurons Revealed by Simultaneous Recordings To directly investigate whether a monosynaptic connection exists between neurons in the rostral ventrolateral (2008)
  • Author(s): OSHIMA N, KUMAGAI H, ONIMARU H, 他
  • Journal Title: Hypertens Res. 31 Pages: 1445-1454
• [Journal Article] Defective interaction between dual oscillators for respiratory rhythm generation in Na+, K+-ATPaseα2 subunit-deficient mice. (2007)
  • Author(s): Onimaru H, Ikeda K, Kawakami K.
  • Journal Title: J Physiol 584.1 Pages: 271284-271284
  • Peer Reviewed
• [Journal Article] Spontaneous Oscillatory Burst Activity in the Piriform-Amygdala Region and its Relation to In Vitro Respiratory Activity in Newborn Rats. (2007)
  • Author(s): Onimaru H, Homma I.
  • Journal Title: Neurosci. 144 Pages: 387-394
  • Peer Reviewed
• [Journal Article] Novel axonal projection from the caudal end of the ventrolateral medulla to the intermediolateral cell column. (2007)
  • Author(s): Iigaya K, Kumagai H, Onimaru H, Kawai A, Oshima N, Onami T, Takimoto C, Kamayachi T, Hayashi K, Saruta T, Itoh H.
  • Journal Title: Am J Physiol Regul Integr Comp Physiol. 292
  • Peer Reviewed
• [Journal Article] Defective interaction between dual oscillators for respiratory rhythm generation in Na^+, K^+-ATPase a2 subunit-deficient mice (2007)
  • Author(s): Onimaru H. Ikeda K, Kawakami K.
  • Journal Title: J Physiol 584.1 Pages: 271-284
  • Peer Reviewed
• [Journal Article] Spontaneous Oscillatory Burst Activity in the Piriform?Amygdala Region and its Relation to in Vitro Respiratory Activity in Newborn Rats (2007)
  • Author(s): Onimaru H, Homma I.
  • Journal Title: Neurosci 144 Pages: 387-394
  • Peer Reviewed
• [Journal Article] Novel axonal projection from the caudal end of the ventrolateral medulla to the intermediolateral cell column (2007)
  • Author(s): Iigaya K, Kumagai H, Onimaru H, ほか
  • Journal Title: Am J Physiol Regul Integr Comp Physiol. 292 Pages: 927-936
  • Peer Reviewed
• [Presentation] Network mechanisms of respiratory rhythm generation in rodents (2009)
  • Author(s): Onimaru, H., Homma, I.
  • Organizer: The 36th Congress of the International Union of Physiological Sciences(IUPS)
  • Place of Presentation: 京都
  • Year and Date: 2009-07-28
• [Presentation] Phox2b expression of pFRG/pre-inspiratory neurons and Respiratory Rhythmogenesis (2009)
  • Author(s): Onimaru, H.
  • Organizer: The XIth Oxford Conference on Modeling and Control of Breathing
  • Place of Presentation: 奈良
  • Year and Date: 2009-07-25
• [Presentation] Phox2b immunoreactivity of the parafacial respiratory group neurons (2009)
  • Author(s): Onimaru, H., Ikeda, K., Kawakami, K.
  • Organizer: The XIth Oxford Conference on. Modeling and Control of Breathing
  • Place of Presentation: 奈良
  • Year and Date: 2009-07-25
• [Presentation] Effects of corticotropin releasing factor on spontaneous burst activity in the piriform-amygdala complex of newborn rat brain preparation in vitro (2009)
  • Author(s): Fujii, T., Onimaru, H., Homma, I.
  • Organizer: The XIth Oxford Conference on Modeling and Control of Breathing
  • Place of Presentation: 奈良
  • Year and Date: 2009-07-25
• [Presentation] Effects of TRP channel agonists on respiratory rhythm generation in the brainstem preparation isolated from newborn rat (2009)
  • Author(s): Suganuma, M., Onimaru, H., Homma I.
  • Organizer: The XIth Oxford Conference on Modeling and Control of Breathing
  • Place of Presentation: 奈良
  • Year and Date: 2009-07-24
• [Presentation] Network mechanisms of respiratory rhythm generation in rodents. (2009)
  • Author(s): Onimaru, H., Homma, I.
  • Organizer: The 36th Congress of the International Union of Physiological Sciences (IUPS) PSJ II-1-1
  • Place of Presentation: Kyoto(シンポジウム招待講演)
• [Presentation] Phox2b expression of pFRG/pre-inspiratory neurons and Respiratory Rhythmogenesis. (2009)
  • Author(s): Onimaru, H.
  • Organizer: The XIth Oxford Conference on Modeling and Control of Breathing, Abstract S5-4
  • Place of Presentation: Nara(シンポジウム講演発表)
• [Presentation] Effects of TRP channel agonists on respiratory rhythm generation in the brainstem preparation isolated from newborn rat. (2009)
  • Author(s): Suganuma, M., Onimaru, H., Homma I.
  • Organizer: The XIth Oxford Conference on Modeling and Control of Breathing, Abstract A34
  • Place of Presentation: Nara
• [Presentation] Phox2b immunoreactivity of the parafacial respiratory group neurons. (2009)
  • Author(s): Onimaru, H., Ikeda, K., Kawakami, K.
  • Organizer: The XIth Oxford Conference on Modeling and Control of Breathing, Abstract B18
  • Place of Presentation: Nara
• [Presentation] Effects of corticotropin releasing factor on spontaneous burst activity in the piriform-amygdala complex of newborn rat brain preparation in vitro. (2009)
  • Author(s): Fujii, T., Onimaru, H., Homma, I.
  • Organizer: The XIth Oxford Conference on Modeling and Control of Breathing, Abstract B11
  • Place of Presentation: Nara
• [Presentation] Postnatal change in the spatio-temporal pattern of respiratory neuron activity in the ventral medulla of rodents (2008)
  • Author(s): Dnimaru., H., Arata, S., Homma, I
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-25
• [Presentation] Characterization and functional significance of parafacial neurons. (2008)
  • Author(s): Onimaru H.
  • Organizer: International Symposium on Respiratory Control.
  • Place of Presentation: St MAXIMIN France(招待講演)
• [Presentation] Postnatal change in the spatio-temporal pattern of respiratory neuron activity in the ventral medulla of rodents. (2008)
  • Author(s): ONIMARU H., ARATA S., HOMMA I.
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] Effects of corticotropin releasing factor (CRF) on spontaneous burst activity in the piriform-amygdala complex of the in vitro brain preparation from newborn rat. Neurosci., Res. (2008)
  • Author(s): Fujii T., Onimaru H., Homma I.
  • Organizer: 第30回日本神経科学会
  • Place of Presentation: 東京
• [Presentation] Characterization and functional significance of parafacial neurons. (2008)
  • Author(s): Onimaru H
  • Organizer: International Symposium on Respiratory Control.
  • Place of Presentation: St MAXIMIN France
• [Presentation] Midline sectiom of the Inedulla abolishes inspiratory activity and desynchronizes pre-inspiratory neuron rhythm on the left and right side of the medulla in newborn rats (2007)
  • Author(s): Onimaru H., Janczewski WA., Homma
  • Organizer: Society for Neuroscience 37th Annual Meeting
  • Place of Presentation: San Diego,USA
  • Year and Date: 2007-11-05
• [Presentation] Effects of midline sagittal sectioning of medulla on pre-inspiratory and inspiratory neuron activity in vitro (2007)
  • Author(s): Onimaru H. and Homma I.
  • Organizer: 第30回日本神経科学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
• [Presentation] Midline section of the medulla abolishes inspiratory activity and desynchronizes pre-inspiratory neuron rhythm on the left and right side of the medulla in newborn rats. (2007)
  • Author(s): Onimaru H., Janczewski WA., Homma I.
  • Organizer: Society for Neuroscience 37th Annual Meeting, Abstract 407. 21
  • Place of Presentation: San Diego, USA
• [Presentation] Opiate- and anoxia-induced respiratory phase shifts in isolated newborn rat brainstems. (2007)
  • Author(s): Ballanyi K., Ruangkittisakul A., Onimaru H.
  • Organizer: Society for Neuroscience 37th Annual Meeting, Abstract 340. 7
  • Place of Presentation: San Diego, USA
• [Presentation] Respiratory rhythm generator dominance in a coupled oscillator system of the medulla. J. Physiol. Sci. (2007)
  • Author(s): Onimaru, H.
  • Organizer: 第84回日本生理学会大会
  • Place of Presentation: 大阪
• [Presentation] Effects of midline sagittal sectioning of medulla on pre-inspiratory and inspiratory neuron activity in vitro. Neurosci. Res. (2007)
  • Author(s): Onimaru H., Homma I.
  • Organizer: 第30回日本神経科学会
  • Place of Presentation: 横浜
• [Book] Adv Exp Med Biol. 669(Phox2b Expressing Neurons in the Most Rostral Medulla of Newborn Rats) (2010)
  • Author(s): Onimaru H, Ikeda K, Kawakami K.
• [Book] Adv Exp Med Biol. 669(Effects of hypocapnia on spontaneous burst activity in the piriform-amygdala complex of newborn rat brain preparation in vitro) (2010)
  • Author(s): Fujii T., Onimaru H., Suganuma M., Homma I.
• [Book] Adv Exp Med Biol. 669 (2010)
  • Author(s): Onimaru H, Ikeda K, Kawakami K.
  • Total Pages: 4
  • Publisher: Springer
• [Book] Adv Exp Med Biol. 669 (2010)
  • Author(s): Fujii T., Onimaru H., Suganuma M., Homma I.
  • Total Pages: 4
  • Publisher: Springer
• [Book] Encyclopedia of Neuroscience(Eds: Marc D. Binder, Nobutaka Hirokawa and Uwe Windhorst, Respiratory Network Analysis, Isolated Respiratory Center Functions) (2009)
  • Author(s): Ballanyi K, Onimaru H.
  • Publisher: Springer-Verlag GmbH
• [Book] Encyclopedia of Neuroscience(Eds: Marc D. Binder, Nobutaka Hirokawa and Uwe Windhorst, Respiratory Network Analysis, Functional Imaging) (2009)
  • Author(s): Ballanyi K, Onimaru H, Keller B.
  • Publisher: Springer-Verlag GmbH
• [Book] Respiratory Network Analysis, Isolated Respiratory Center Functions. Encyclopedia of Neuroscience. (2009)
  • Author(s): Ballanyi K, Onimaru H.
  • Total Pages: 5
  • Publisher: Springer-Verlag GmbH Berlin Heidelberg
• [Book] Respiratory Network Analysis, Functional Imaging. Encyclopedia of Neuroscience (2009)
  • Author(s): Ballanyi K, Onimaru H, Keller B.
  • Total Pages: 5
  • Publisher: Springer-Verlag GmbH Berlin Heidelberg
• [Book] Advances in Experimental Medicine and Biology Series Volume 605(Eds M. J. Poulin and R. J. A. Wilson, Newborn Rat Brainstem-Spinal Cord Preparation) (2008)
  • Author(s): Onimaru H
  • Publisher: Springer Publishers
• [Book] Two Modes of Respiratory Rhythm Generation in the Newborn Rat Brainstem-Spinal Cord Preparation.In Advances in Experimental Medicine and Biology Series (2008)
  • Author(s): Onimaru H. Homma I.
  • Total Pages: 5
  • Publisher: Springer Publishers, New York