Cellular immunological activities of antigenic N-glycans from unused seaweed-glycoproteins and it's applications.

• Project/Area Number: 19500701
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Sakuyo Music College
• Principal Investigator: KIMURA Mariko Sakuyo Music College, 食文化学部, 准教授 (00351932)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 海藻 / 生理活性 / 抗原性 / 糖鎖 / サイトカイン / 未利用資源 / 糖タンパク質糖鎖 / 抗原性糖鎖 / 海藻糖タンパク質 / 糖鎖薬剤 / 機能性食品 / 糖鎖構造 / 海藻N-グリカン

Research Abstract

In this study, we analyzed the structures and cellular immunological activities of N-glycans linked to seaweed glycoproteins. First, glycopeptides were prepared from the pepsin-digests of seaweed homogenates (brown algae, red seaweeds, and green laver) by gelfiltration and HPLC. Glycan moieties were liberated by hydrazinolysis and then were fluorescence-labeled. The structural analysis using HPLC-mapping showed that major N-glycans of hydrophytes are typical high-mannose type structures, while the typical plant complex type N-glycans having α1-3Fuc and β1-2 Xyl residues were not found. However, we found some unique glycans that were resistant to several glycosidases, indicating that some hydrophytes-specific glycans might occur on some seaweed glycoproteins. Furthermore, the immunological activity of the seaweed glycopeptides was assayed, and it was found that some glycopeptide-fraction could stimulate human monocytes.

Research Products

• [Journal Article] N-Glycans linked to glycoproteins in edible beans : Natural resources for bioactive oligosaccharides. (2011)
  • Author(s): Kimura M., Hara T., Kimura Y.
  • Journal Title: Biosci.Biotechnol.Biochem. 75(1) Pages: 155-158
  • Peer Reviewed
• [Journal Article] N-Glycans linked to glycoproteins in edible beans : Natural Resources for bioactive oligosaccharides. (2011)
  • Author(s): Mariko Kimura
  • Journal Title: Biosci.Biotechnol.Biochem. Volume: 75 Pages: 155-158
  • Peer Reviewed
• [Journal Article] Intracellular and extracellular free N-glycans produced by plant cells : Occurrence of unusual plant complex-type free N-glycans in extracellular spaces. (2010)
  • Author(s): Maeda M., Kimura M., Kimura Y.
  • Journal Title: J.Biochem. 148(6) Pages: 681-692
  • NAID: 10027879797
  • Peer Reviewed
• [Journal Article] Identification of royal jelly glyco-protein that carries unique complex type N-glycans harboring T-antigen(Galβ1-3GalNAc)unit. (2010)
  • Author(s): Kimura Y., Nagai H., Miyamoto M., Kimura M., Yonekura M.
  • Journal Title: Biosci.Biotechnol.Biochem. 74(10) Pages: 2148-2150
  • NAID: 10027560979
  • Peer Reviewed
• [Journal Article] Intracellular and extracellular free N-glycans produced by plant cells : occurrence of unusual plant complex type free N-glycans in extracellular spaces. (2010)
  • Author(s): Megumi Maeda
  • Journal Title: J.Biochem. Volume: 148 Pages: 603-616
  • Peer Reviewed
• [Journal Article] Identification of royal jelly glycoprotein that carries unique complex type N-glycans harboring T-antigen(Galβ1-3GalNAc)unit. (2010)
  • Author(s): Yoshinobu Kimura
  • Journal Title: Biosci.Biotechnol.Biochem. Volume: 74 Pages: 2148-2150
  • NAID: 10027560979
  • Peer Reviewed
• [Journal Article] Salt-adaptation of Tobacco BY2 cells induces change in glycoform of N-glycans. (2008)
  • Author(s): Kimura Y., Watanabe T., Kimura M., Maeada M., Murata Y., Fujiyama K.
  • Journal Title: Biosci.Biotechnol.Biochem 72 Pages: 514-522
  • Peer Reviewed
• [Journal Article] Salt-adaptation of Tobacco BY2 cells induces change in glycoform of N-glycans (2008)
  • Author(s): Kimura, Y., Watanabe, T., Kimura, M., Maeada, M., Murata, Y., Fujiyama, K.
  • Journal Title: Biosci. Biotechnol. Biochem 72 Pages: 514-522
  • Peer Reviewed
• [Journal Article] Salt-adaptation of Tobacco BY2 cells induces change in glycoform of N-glycans : Enhancement of exo-and endo-glycosidaseactivities by salt-adaptation. (2008)
  • Author(s): Kimura, Y., Watanabe, T., Kimura, M., Maeada, M., Murata, Y., Fujiyama, K.
  • Journal Title: Biosci. Biotechnol. Biochem. 72 Pages: 514-522
  • Peer Reviewed
• [Journal Article] Salt-adaptation of Tobacco BY2 cells induces change in glycaform of N-glycans (2008)
  • Author(s): Kimura, Y., Watanabe, T., Kimura. M., Maeada, M., Murata, Y., Fuiivama, K.
  • Journal Title: Biosci.Biotechnol.Biochem 72 Pages: 514-522
  • Peer Reviewed
• [Presentation] Sppression of Endo-β-N-acetylglucosaminidase and Peptide : N-glycanase Activity in Arabidopsis thaliana (2009)
  • Author(s): Takeoka, Y., Sakamura, S., Fujiyama, K., Kimura, M., Kimura, Y.
  • Organizer: 15th European Carbohydrate Symposium
  • Place of Presentation: Vienna(Austria)
  • Year and Date: 2009-07-20
• [Presentation] A novel β1-3-galactosyltransferase involved in biosynthesis of honeybee N-glycan bearing T-antigen unit (2009)
  • Author(s): Sakamura, S., Kimura, M., Fujiyama, K., Kimura, Y.
  • Organizer: 15th European Carbohydrate Symposium
  • Place of Presentation: Vienna(Austria)
  • Year and Date: 2009-07-20
• [Presentation] A novel β1-3 galactosyltransferase involved in biosynthesis of honeybee N-glycan bearing T-antigen unit. (2009)
  • Author(s): Sakamura S., Kimura M., Fujiyama K., Okihara K., Hashimoto K., Yamada H., Kimura Y.
  • Organizer: 15^<th> European Carbohydrate Symposium, Abstract p 487
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2009-07-19
• [Presentation] Suppression of endo-β-N-acetyl-glucosaminidase and peptide : N-glycanase activity in Arabidopsis thaliana. (2009)
  • Author(s): Takeoka Y., Sakamuira S., Fujiyama K., Kimura M., Kimura Y.
  • Organizer: 15^<th> European Carbo-hydrate Symposium, Abstract p 531
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2009-07-19
• [Presentation] A novel β 1-3 galactosyltransferase involved in biosynthesis of honeybee N-glycan bearing T-antigen unit (2009)
  • Author(s): Sakamura, S., Kimura, M., Fujiyama, K., Okihara, K., Hashimoto, K., Yamada, H., Kimura, Y.
  • Organizer: 15^<th> European Carbohydrate Symposium, Abstract p487
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2009-07-19
• [Presentation] Suppression of endo-β-N-acetyl-glucosaminidase and peptide : N-glycanase activity in Arabidopsis thaliana (2009)
  • Author(s): Takeoka, Y., Sakamuira, S., Fujiyama, K., Kimura, M., Kimura, Y.
  • Organizer: 15^<th> European Carbo- hydrate Symposium, Abstract p 531
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2009-07-19
• [Presentation] 糖タンパク質薬剤開発に必要とされる有用糖ペプチドの多量調製法の確立 (2009)
  • Author(s): 永井裕美,木村万里子,木村吉伸
  • Organizer: 日本農芸化学会大会(講演要旨集p323)
  • Place of Presentation: 福岡
• [Presentation] 糖タンパク質薬剤開発に必要とされる有用糖ペプチドの多量調製法の確立 (2009)
  • Author(s): 永井裕美, 木村万里子, 木村吉伸
  • Organizer: 日本農芸化学会大会, 講演要旨集p323
  • Place of Presentation: 福岡
• [Presentation] Suppression of Endo-β-N-acetylglucosaminidase Activity in Arabidopsis thaliana and Tomato Plant. (2008)
  • Author(s): Nakamura, K., Takeoka, Y., Sakamura, S., Hosoi, K., Fujiyama, K., Kimura, M., Kimura, Y.
  • Organizer: International Carbohydrate Symposium 2008
  • Place of Presentation: ノルウェー, オスロ
  • Year and Date: 2008-07-28
• [Presentation] Suppression of Endo-β-N-acetyl glucosaminidase activity in Arabidopsis thaliana and Tomato Plant. (2008)
  • Author(s): Nakamura K., Takeoka Y., Sakamura S., Hosoi K., Fujiyama K., Kimura M., Kimura Y.
  • Organizer: XXIV International Carbohydrate Symposium
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2008-07-27
• [Presentation] Suppression of Endo-β-N-acetyl glucosaminidase activity in Arabidopsis thaliana and Tomato Plant (2008)
  • Author(s): Nakamura, K., Takeoka, Y., Sakamura, S., Hosoi, K., Fujiyama, K., Kimura, M., Kimura, Y.
  • Organizer: XXIV International Carbohydrate Symposium
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2008-07-27
• [Presentation] 雑豆に含まれる糖タンパク質糖鎖の網羅的構造解析 (2008)
  • Author(s): 原共紀、木村万里子、木村吉伸
  • Organizer: 日本農芸化学会中四国支部例会
  • Place of Presentation: 徳島
• [Presentation] Processing pathway of N-glycan with T-antigen in Apis mellifera : Structural features of N-glycans synthesized in Apis Mellifera (2007)
  • Author(s): Takayuki U., Mariko K., Yoichiro H., Kiyoshi O., Hiroyuki S., Hideo Y., Yoshinobu K.
  • Organizer: XIX International Symposium on Glycoconjugates
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2007-07-19
• [Presentation] Identification of royal jelly glyco-protein bearing N-glycans with T-antigen unit. (2007)
  • Author(s): Hiromi N., Mariko K., Kiyoshi O., Ken H., Hiroyuki S., Hideo Y., Masami Y., Yoshinobu K.
  • Organizer: XIX International Symposium on Glycoconjugates
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2007-07-19
• [Presentation] Identification of royal jelly glyco-protein bearing N-glycans with T- antigen unit (2007)
  • Author(s): Hiromi N., Mariko K., Kiyoshi O., Ken H., Hiroyuki S., Hideo Y., Masami Y., Yoshinobu K.
  • Organizer: XIX International Symposium on Glycoconjugates
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2007-07-19
• [Presentation] Identification of royal jelly glycoprotein bearing N-glycans with Tantlgen unit (2007)
  • Author(s): Hiromi N., Mariko K., Kiyoshi O., Ken H., Hiroyuki s., Hideo Y., Masami Y., Yoshinobu K
  • Organizer: XIX International Symposium on Glycoconjugates
  • Place of Presentation: ケアンズ・オーストラリア
  • Year and Date: 2007-07-17
• [Presentation] Processing pathway of N-glycan with T-antigen in Apis mellifera:Structural features of N-glycans synthesized in Apis mellifera (2007)
  • Author(s): Takayuki U., Mariko K., Yoichiro H., Kiyoshi O., Hiroyuki S., Hideo Y., Yoshinobu K.
  • Organizer: XIX International Symposium on Glycoconjugates
  • Place of Presentation: ケアンズ・オーストラリア
  • Year and Date: 2007-07-17
• [Presentation] 雑豆に含まれる糖タンパク質糖鎖の網羅的構造解析 (2007)
  • Author(s): 原 共紀、木村 万里子、木村 吉伸
  • Organizer: 日本農芸化学会中四国支部例会
  • Place of Presentation: 徳島大学
  • Year and Date: 2007-01-26
• [Book] 水産食品(魚介類・藻類) (「食べ物と健康」食品学・食品機能学・食品加工学(改訂版)) (2010)
  • Author(s): 長澤治子,木村万里子, 他
  • Publisher: 医歯薬出版
• [Book] 食品学実験-基礎から応用まで-(改訂版) (2010)
  • Author(s): 長澤治子,木村万里子, 他
  • Publisher: 青山社
• [Book] 「食べ物と健康」食品学・食品機能学・食品加工学 (改訂版) (2010)
  • Author(s): 長澤治子, 木村万里子, 他
  • Publisher: 医歯薬出版
• [Book] 「食品学実験―基礎から応用まで―」(改訂版) (2010)
  • Author(s): 長澤治子, 木村万里子, 他
  • Publisher: 青山社
• [Book] 食品学実験-基礎から応用まで (2007)
  • Author(s): 長澤 治子、塩見 慎次郎, 額田 真喜子, 川口 洋、木村 万里子, 山本 奈美
  • Total Pages: 227
  • Publisher: 青山社