Analysis of DNA damage avoidance systems by RNA polymerase II on transcription elongation

• Project/Area Number: 19510064
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Osaka University (2008); Department of Clinical Research, National Hospital Organization National Kyushu Cancer Center (2007)
• Principal Investigator: KURAOKA Isao Osaka University, 大学院・基礎工学研究科, 准教授 (60335396)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: DNA損傷 / DNA修復 / 突然変異機構 / 転写機構 / DNA修復機構 / RNAポリメラーゼ / メチル化損傷 / 転写変異 / ヌクレオチド除去修復機構 / 塩基除去修復機構 / RNAポリメラーゼII / TFIIS / XAB2 / スプライシング

Research Abstract

転写反応が活性酸素で生じるDNA損傷に遭遇したときの挙動を解析し、転写における損傷回避機構(損傷乗り越えRNA合成) を発見した。また色素性乾皮症A群結合蛋白質が細胞内で蛋白質複合体を形成し、これがスプライシング因子として機能すること、さらにこれに加えて色素性乾皮症G群蛋白質が転写因子と蛋白質複合体を形成し、基本転写に直接関与することを見いだした。

Research Products

• [Journal Article] Effects of DNA lesions on transcription elongation by RNA polymerases (2008)
  • Author(s): Kuraoka, I.
  • Journal Title: Genes Environ 30 Pages: 63-70
  • NAID: 110006873842
  • Peer Reviewed
• [Journal Article] Isolation of XAB2 complex involved in pre-mRNA splicing, transcription, and transcription-coupled repair (2008)
  • Author(s): Kuraoka, I., Ito, S., Wada, T., Hayashida, M., Lee, L., Saijo, M., Nakatsu, Y., Matsumoto, M., Matsunaga, T., Handa, H., Qin, J., Nakatani, Y., and Tanaka, K.
  • Journal Title: J Biol Chem 283 Pages: 940-950
  • Peer Reviewed
• [Journal Article] Effects of DNA lesions on Transcription Elongation by RNA polymerases. (2008)
  • Author(s): Kuraoka Isao
  • Journal Title: Genes and Environment 30 Pages: 63-70
  • NAID: 110006873842
  • Peer Reviewed
• [Journal Article] Isolation of XAB2 complex involved in pre-mRNA splicing, transcription, and transcription-coupled repair. (2008)
  • Author(s): Kuraoka I, Ito S, Wada T, Hayashida M, Lee L, Saijo M, Nakatsu Y, Matsumoto M, Matsunaga T, Handa H, Qin J, Nakatani Y, Tanaka K.
  • Journal Title: J Biol Chem. 283 Pages: 940-50
  • Peer Reviewed
• [Journal Article] DNA repair as a determinant of tumour chemosensitivity (2007)
  • Author(s): Oda, S., Kuraoka, I., and Maehara, Y.
  • Journal Title: Gan To Kagaku Ryoho 34 Pages: 347-357
• [Journal Article] RNA polymerase II bypasses 8-oxoguanine in the presence of transcription elongation factor TFIIS (2007)
  • Author(s): Kuraoka, I., Suzuki, K., Ito, S., Hayashida, M., Kwei, J. S., Ikegami, T., Handa, H.,Nakabeppu, Y., and Tanaka, K.
  • Journal Title: DNA Repair(Amst) 6 Pages: 841-851
  • Peer Reviewed
• [Journal Article] Molecular evidence of the involvement of the nucleotide excision repair (NER) system in the repair of the mono(ADP-ribosyl)ated DNA adduct produced by pierisin-1, an apoptosis-inducing protein from the cabbage butterfly (2007)
  • Author(s): Kawanishi, M., Matsukawa, K., Kuraoka, I., Takamura-Enya, T., Totsuka, Y., Matsumoto, Y., Watanabe, M., Zou, Y., Tanaka, K., Sugimura, T., Wakabayashi, K., and Yagi, T.
  • Journal Title: Chem Res Toxicol 20 Pages: 694-700
  • Peer Reviewed
• [Journal Article] XPG stabilizes TFIIH, allowing transactivation of nuclear receptors : implications for Cockayne syndrome in XP-G/CS patients (2007)
  • Author(s): Ito, S., Kuraoka, I., Chymkowitch, P., Compe, E., Takedachi, A., Ishigami, C., Coin, F., Egly, J. M., and Tanaka, K.
  • Journal Title: Mol Cell 26 Pages: 231-243
  • Peer Reviewed
• [Journal Article] DNA damage-dependent acetylation and ubiquitination of H2AX enhances chromatin dynamics (2007)
  • Author(s): Ikura, T., Tashiro, S., Kakino, A., Shima, H., Jacob, N., Amunugama, R., Yoder, K., Izumi, S., Kuraoka, I., Tanaka, K., Kimura, H., Ikura, M., Nishikubo, S., Ito, T., Muto, A., Miyagawa, K., Takeda, S., Fishel, R., Igarashi, K., and Kamiya, K.
  • Journal Title: Mol Cell Biol 27 Pages: 7028-7040
  • Peer Reviewed
• [Journal Article] RNA polymerase II bypasses 8-oxoguanine in the presence of transcription elongation factor TFIIS. (2007)
  • Author(s): Kuraoka I, Suzuki K, Ito S, Hayashida M, Kwei JS, Ikegami T, Handa H, Nakabeppu Y, Tanaka K.
  • Journal Title: DNA Repair 6 Pages: 841-51
  • Peer Reviewed
• [Journal Article] XPG stabilizes TFIIH, allowing transactivation of nuclear receptors : implications for Cockayne syndrome in XP-G/CS patients. (2007)
  • Author(s): Ito S, Kuraoka I, Chymkowitch P, Compe E, Takedachi A, Ishigami C, Coin F, Egly JM, Tanaka K.
  • Journal Title: Mol Cell. 26 Pages: 7028-40
  • Peer Reviewed
• [Presentation] ヒトRNAポリメラーゼII転写反応におけるメチル化DNA損傷の影響 (2008)
  • Author(s): 倉岡功、田中亀代次
  • Organizer: 第37回日本環境変異原学会
  • Place of Presentation: 宜野湾
  • Year and Date: 2008-12-05
• [Presentation] 転写およびスプライシングに関与する修復蛋白質XAB2複合体の単離 (2008)
  • Author(s): 倉岡功、田中亀代次
  • Organizer: 第51回日本放射線影響学会
  • Place of Presentation: 北九州
  • Year and Date: 2008-11-21
• [Presentation] Effects of methyl DNA lesions on transcription elongation by RNA polymerase II (2008)
  • Author(s): Kuraoka I
  • Organizer: Gordon ResearchConferences (Mutagenesis)
  • Place of Presentation: Oxford
  • Year and Date: 2008-07-22
• [Presentation] Effects of methyl DNA lesions on transcription elongation by RNA polymerase II (2008)
  • Author(s): Kuraoka Isao
  • Organizer: Gordon Research Conferences (Mutagenesis)
  • Place of Presentation: オックスフォード大学
• [Presentation] 転写およびスプライシングに関与する修復蛋白質XAB2複合体の単離 (2008)
  • Author(s): 倉岡 功, 田中亀代次
  • Organizer: 日本放射線影響学会第51回大会
  • Place of Presentation: 北九州国際会議場
• [Presentation] ヒトRNAポリメラーゼII転写反応におけるメチル化DNA損傷の影響 (2008)
  • Author(s): 倉岡 功, 田中亀代次
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄コンベンションセンター
• [Presentation] メチル化によるDNA 損傷がRNAPII の転写伸長に与える影響 (2007)
  • Author(s): 倉岡功、田中亀代次
  • Organizer: 第30回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] メチル化によるDNA損傷がRNAPIIの転写伸長に与える影響 (2007)
  • Author(s): 倉岡 功、田中亀代次
  • Organizer: 第30回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] 色素性乾皮症G群蛋白質は基本転写因子TFIIHと複合体を形成する (2007)
  • Author(s): 倉岡功、伊藤伸介、竹立新人、石上智愛、田中亀代次
  • Organizer: 第36回日本環境変異原学会
  • Place of Presentation: 北九州
  • Year and Date: 2007-11-30
• [Presentation] 色素性乾皮症G群蛋白質は基本転写因子TFIIH複合体の安定化する (2007)
  • Author(s): 倉岡功、伊藤伸介、竹立新人、石上智愛、田中亀代次
  • Organizer: 第50回日本放射線影響学会
  • Place of Presentation: 千葉
  • Year and Date: 2007-11-15
• [Presentation] RNAポリメラーゼII 転写伸長反応におけるDNA塩基損傷の影響 (2007)
  • Author(s): 倉岡功、田中亀代次
  • Organizer: 第66回日本癌学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-04
• [Presentation] RNAポリメラーゼII転写伸長反応におけるDNA塩基損傷の影響 (2007)
  • Author(s): 倉岡 功、田中亀代次
  • Organizer: 第66回日本癌学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-04
• [Presentation] 色素性乾皮症G群蛋白質による基本転写因子TFIIH複合体の安定化 (2007)
  • Author(s): 倉岡功、伊藤伸介、竹立新人、田中亀代次
  • Organizer: 変異機構研究会第20回夏の学校
  • Place of Presentation: 名古屋
  • Year and Date: 2007-07-29
• [Presentation] 色素性乾皮症G群蛋白質は基本転写因子TFIIHと複合体を形成する (2007)
  • Author(s): 倉岡功、伊藤伸介、竹立新人、田中亀代次
  • Organizer: 第13回日本家族性腫瘍学会
  • Place of Presentation: 高知
  • Year and Date: 2007-06-16