Antioxidative activity of uric acid in Drosophila

• Project/Area Number: 19510067
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Okayama University
• Principal Investigator: 根岸 友恵 (根岸 友惠) Okayama University, 大学院・医歯薬学総合研究科, 准教授 (80116491)
• Co-Investigator(Kenkyū-buntansha): 鈴木 利典 就実大学, 薬学部, 教授 (90368697)
• Research Collaborator: 濱武 有子 岡山大学, 大学院・医歯薬学総合研究科, 博士前期課程・大学院生 ; 藤原 大 岡山大学, 大学院・医歯薬学総合研究科, 博士前期課程・大学院生
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 尿酸 / 活性酸素 / ショウジョウバエ / タバコ副流煙 / 酸化傷害 / 生殖毒性 / ニトロソ化

Research Abstract

痛風の原因物質として知られている尿酸であるが、抗酸化物質として重要な働きをしている。尿酸がどのような酸化ストレスに対して防御作用を示すかを調べ、生物における尿酸の存在意義とその利用価値を示すことを目的とした。ショウジョウバエの尿酸欠損株はタバコ副流煙曝露に感受性が高い。副流煙曝露時の尿酸含量を測定した結果、野生株では尿酸が、尿酸欠損株では前駆体含量は増加した。このことは酸化傷害に対する防御機構として尿酸合成が亢進している可能性を示唆するものである。

Research Products

• [Journal Article] Acetylation to amino group on guanosine induced by nitric oxide inn acetonitrile under aerobic conditions (2009)
  • Author(s): Suzuki, T., Fukai, T., Seki, Y., Inukai, M.
  • Journal Title: Chem.Pharm.Bull. 57 Pages: 89-91
• [Journal Article] Formation of diazoate intemediate upon nitrous acid and nitric oxide treatment of 2'-deoxyadenosine (2009)
  • Author(s): Suzuki, T., Iwakura, K., Takashima, Y., Kasajima, N., Inukai, M.
  • Journal Title: Bioorg.Med.Chem. 19 Pages: 788-791
• [Journal Article] Hypersensitivity of a urate-null strain of Drosophila melanogaster to the toxic effects of environmental cigarette smoke (2009)
  • Author(s): Hamatake, Y., Morita, A., Yuma, Y., Okamoto, K., Arimoto, S., Suzuki, T., Kasai, H., Kawai, K., Negishi, T.
  • Journal Title: Genes & Environment 31 Pages: 43-45
  • NAID: 10025925947
• [Journal Article] Formation of spiroiminodihydantoin nucleoside from 8-oxo-7, 8-dihydro-2'-deoxyguanosine by nitric oxide under aerobic conditions (2009)
  • Author(s): Suzuki, T.
  • Journal Title: Bioorg.Med.Chem.Lett. 19 Pages: 4944-4947
• [Journal Article] Effects of uric acid on nitrosation of N-acetylcysteine by diethylamine NONOate and N-acetyl-N-nitrosotryptophan (2009)
  • Author(s): Suzuki, T., Naka, A., Kimura H.
  • Journal Title: Chem.Pharm.Bull. 57 Pages: 736-739
• [Journal Article] Effects of bromide upon reaction of nucleosides with hydrogen peroxide induced by ultraviolet light (2009)
  • Author(s): Suzuki, T., Moriwaki, N., Kurokawa, K., Inukai, M.
  • Journal Title: Bioorg.Med.Chem.Lett. 9 Pages: 3217-3219
• [Journal Article] Acetylation to amino group on guanosine induced by nitric oxide inacetonitrile under aerobic conditions. (2009)
  • Author(s): Suzuki, T., et al.
  • Journal Title: Chem. Phrm. Bull. 57 Pages: 89-91
  • Peer Reviewed
• [Journal Article] Formation of diazoate intermediate upon nitrous acid and nitric oxidetreatment of 2' -deoxyadenosine. (2009)
  • Author(s): Suzuki, T., et al.
  • Journal Title: Bioorg. Med. Chem. 19 Pages: 788-791
  • Peer Reviewed
• [Journal Article] Hypersensitivity of a urate-null strain of Drosophila melanogaster to the toxic effects of environmental cigarette smoke (2009)
  • Author(s): Hamatake, Y., et al.
  • Journal Title: Genes & Envirnment in press
  • Peer Reviewed
• [Journal Article] Binding of MutS protein to oligonucleotides containing a methylated- or an ethylated guanine residue, and correlation with mutation frequency (2008)
  • Author(s): Taira, K., Nakamura, S., Nakano, K., Maehara, D., Okamoto, K., Arimoto, S., Loakes, D., Worth, L., Schaaper, R. M., Seio, K., Sekine, M., Negishi, K., Negishi, T.
  • Journal Title: Mutat.Res. 640 Pages: 107-112
• [Journal Article] Reaction of 2'-deoxicytidine with peroxynitrite in the presence of ammonium bromide (2008)
  • Author(s): Suzuki. T., Iida, K., Uchibe, S., Inukai, M.
  • Journal Title: Bioorg.Med.Chem. 16 Pages: 5164-5170
• [Journal Article] Influence of neighboring base sequences on the mutagenesis induced by 7,8-dihydro-8-oxoguanine in yeast (2008)
  • Author(s): Yung, C.-W., Okugawa, Y., Otsuka, C., Okamoto, K., Arimoto, S., Loakes, D., Negishi, K., Negishi, T.
  • Journal Title: Mutagenesis 23 Pages: 509-513
• [Journal Article] Kinetics of dCTP incorporation opposite to 7, 8-dihydro-8-oxoguanine with different 5'nearest neighbors by yeast polymerase h (2008)
  • Author(s): Yung, C. -W., et al.
  • Journal Title: Nucleic Acids Symposium Series 52 Pages: 531-532
• [Journal Article] Influence of neighboring base sequences on the mutagenesis induced by 7, 8-dihydro-8-oxoguanine in yeast (2008)
  • Author(s): Yung, C. -W., et al.
  • Journal Title: Mutagenesis 23 Pages: 509-513
  • Peer Reviewed
• [Journal Article] Reaction of 2' -deoxycytidine with peroxynitrite in the presence of ammonium bromide. (2008)
  • Author(s): Suzuki, T., et al.
  • Journal Title: Bioorg. Med. Chem. 16 Pages: 5164-5170
  • Peer Reviewed
• [Journal Article] Binding of MutS protein to oligonucleotides containing a methylated- or an ethylated guanine residue, and correlation with mutation frequency (2008)
  • Author(s): Taira, K., et. al.
  • Journal Title: Mutation Res. 640 Pages: 107-112
  • Peer Reviewed
• [Journal Article] Reaction of 2'-deoxycytidine with peroxynitrite in the presence of ammonium bromide (2008)
  • Author(s): Suzuki, T., et. al.
  • Journal Title: Bioorg. Med. Chem (in press)
  • Peer Reviewed
• [Journal Article] Enhancement of phase II enzyme activity by purpurin resulting in suppression of MeIQx-DNA adduct formation in mice (2007)
  • Author(s): Takahashi, E., Arimoto, S., Okamoto, K., Negishi. T.
  • Journal Title: Mutat.Res. 626 Pages: 128-134
• [Journal Article] Levels of 8-hydroxy-2'-deoxyguanosine increase in Drosophila larval DNA after irradiation with 364nm laser-light but not X-ray (2007)
  • Author(s): Negishi, T., Kawai, K., Arakawa, R., Higashi, H., Nakamura, T., Watanabe, M., Kasai, H., Fujikawa, K.
  • Journal Title: Photochem. Photobiol. 83 Pages: 658-663
• [Journal Article] Nitrosation of uric acid induced by nitric oxide under aerobic conditions (2007)
  • Author(s): Suzuki, T.
  • Journal Title: Niric Oxide 16 Pages: 226-273
• [Journal Article] Levels of 8-hydroxy-2'-deoxyguanosine increase in Drosophila larval DNA after irradiation with 364 nm laser-light but not X-ray (2007)
  • Author(s): Negishi, T., et. al.
  • Journal Title: Photochem. Photobiol. 83 Pages: 658-663
  • Peer Reviewed
• [Journal Article] Enhancement of phase II enzyme activity by purpurin resulting in suppression of MeIQx-DNA adduct formation in mice (2007)
  • Author(s): Takahashi, E., et. al.
  • Journal Title: Mutation Res. 626 Pages: 128-134
  • Peer Reviewed
• [Journal Article] Nucleotide incorporation against 7,8-dihydro-8-oxoguanine is influenced by neighboring base sequences in TLS DNA polymerase reaction (2007)
  • Author(s): Yung, C.W., et. al.
  • Journal Title: Nucleic Acids Symposium Series 51 Pages: 49-50
• [Journal Article] Nitrosation of uric acid induced by nitric oxide under aerobic conditions (2007)
  • Author(s): Suzuki, T.
  • Journal Title: Nitric Oxide 16 Pages: 266-273
  • Peer Reviewed
• [Presentation] 副流煙曝露により誘起される致死作用と尿酸含量の関係 (2009)
  • Author(s): 藤原大, 濱武有子, 岡本敬の介, 有元佐賀惠, 鈴木利典, 根岸友惠
  • Organizer: 日本薬学会129年会
  • Place of Presentation: 京都
• [Presentation] ヤナギマツタケに含まれる抗変異原物質の研究 (2009)
  • Author(s): 米田香仁, 塩澤明子, 岡本敬の介, 有元佐賀惠, 根岸友惠
  • Organizer: 日本薬学会129年会
  • Place of Presentation: 京都
• [Presentation] システインとトリプトファンのニトロン化反応における尿酸の作用 (2009)
  • Author(s): 鈴木利典, 仲敦史, 木村悠
  • Organizer: 日本薬学会129年会
  • Place of Presentation: 京都
• [Presentation] システインとトリプトファンのニトロソ化反応における尿酸の作用 (2009)
  • Author(s): 鈴木 利典, 他
  • Organizer: 日本薬学会129年会
  • Place of Presentation: 京都
• [Presentation] タバコ副流煙の生体影響 (2008)
  • Author(s): 濱武有子, 森田あゆみ, 岡本敬の介, 有元佐賀恵, 葛西宏, 河井一明, 鈴木利典, 根岸友恵
  • Organizer: 日本薬学会128年会
  • Place of Presentation: 横浜
• [Presentation] 酵母ポリメラーゼη欠損株における8-ヒドロキシグアニンの変異誘導に対する隣接配列の影響 (2008)
  • Author(s): 根岸和雄, 川上朝子, 大塚智恵, 翁經緯, 尾山廣, 根岸友惠
  • Organizer: 日本薬学会128年会
  • Place of Presentation: 横浜
• [Presentation] アルキル化剤の変異原性に対するミスマッチ修復の関与 (2008)
  • Author(s): 中野浩太, 平健太郎, 有元佐賀惠, 岡本敬の介, 根岸友惠
  • Organizer: 日本薬学会128年会
  • Place of Presentation: 横浜
• [Presentation] 8-Oxo 残基の translesion synthesis における隣接塩基配列の影響 (2008)
  • Author(s): 翁經緯, 奥川洋司, 川上朝子, 岡本敬の介, 有元佐賀惠, David Loakes, 根岸和雄, 根岸友惠
  • Organizer: 日本薬学会128年会
  • Place of Presentation: 横浜
• [Presentation] 臭化アンモニウム存在下でのパーオキシナイトライトとデオキシシチジンの反応 (2008)
  • Author(s): 鈴木利典, 位田和也
  • Organizer: 日本薬学会128年会
  • Place of Presentation: 横浜
• [Presentation] UVAレーザーによるDNAの酸化傷害 (2008)
  • Author(s): 根岸友惠, 東正一, 中村貴宣, 渡辺正勝, 葛西宏, 河井一明, 翁經緯, 根岸和雄
  • Organizer: 第30回日本光医学・光生物学会
  • Place of Presentation: 松江
• [Presentation] ヤナギマツタケに含まれる抗変異原物質の研究 (2008)
  • Author(s): 米田香仁, 塩澤明子, 有元佐賀惠, 岡本敬の介, 根岸友惠
  • Organizer: 第13回日本フードファクター学会
  • Place of Presentation: 東京
• [Presentation] 副流煙曝露により誘起される致死作用と尿酸含量の関係 (2008)
  • Author(s): 根岸友惠, 濱武有子, 藤原大, 有元佐賀惠, 鈴木利典
  • Organizer: 第37回日本環境変異原学会
  • Place of Presentation: 那覇
• [Presentation] O^4-alkylthymine の修復経路の解明 (2008)
  • Author(s): 中野浩太, 高橋栄造, 有元佐賀惠, 岡本敬の介, 根岸友惠
  • Organizer: 第37回日本環境変異原学会
  • Place of Presentation: 沖縄
• [Presentation] Kinetics of dCTP incorporation opposite to 7, 8-dihydro-8-oxoguanine with different 5' nearest neighbos by yeast polymerase h (2008)
  • Author(s): Yung, C. -W., et al
  • Organizer: Joint Symposium of the 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and the 35th international Symposiumu on Nucleic Acids Chemistry
  • Place of Presentation: 京都
• [Presentation] 水、窒素酸化物、ハロゲンが関与するDNA損傷 (2008)
  • Author(s): 鈴木 利典
  • Organizer: 第21回変異機構研究会
  • Place of Presentation: 小牧(愛知県)
• [Presentation] Effects of environmental cigarette smoke (ECS)on the viability of Drosophila exposed at the third instar larval stage and urate contents inlarvae : 副流煙曝露により誘起される致死作用と尿酸含量の関係 (2008)
  • Author(s): 根岸 友恵, 他
  • Organizer: 第37回日本環境変異原学会
  • Place of Presentation: 那覇
• [Presentation] Repair pathway of 04-alkylthymine : 04-alkylthymineの修復経路の解明 (2008)
  • Author(s): 中野 浩太, 他
  • Organizer: 第37回日本環境変異原学会
  • Place of Presentation: 那覇
• [Presentation] タバコ副流煙の生体影響 (2008)
  • Author(s): 濱武 有子, 他
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Presentation] N-ニトロソプロリンとデオキシグアノシとのUVA光反応生成物 (2008)
  • Author(s): 佐野 嘉容子, 他
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Presentation] 8-oxo残基のtranslesion synthesisにおける隣接塩基配列の影響 (2008)
  • Author(s): 翁 經緯, 他
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Presentation] アルキル化剤の変異原生に対するミスマッチ修復の関与 (2008)
  • Author(s): 中野 浩太, 他
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Presentation] 臭化アンモニウム存在下でのパーオキシナイトライトとデオキシシチジンの反応 (2008)
  • Author(s): 鈴木 利典, 他
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Presentation] 臭化アンモニウム存在下でのパーオキシナイトライトとデオキシシチジンの反応 (2007)
  • Author(s): 鈴木利典, 内部慎也
  • Organizer: 第29回日本フリーラジカル学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] Nucleotide incorporation against 7,8-dihydro-8-oxoguanine is influenced y neighboring base sequences in TLS DNA polymerase reaction (2007)
  • Author(s): 翁經緯, 鈴木哲矢, 川上朝子 Loakes, D., 根岸和雄, 根岸友惠
  • Organizer: 日本環境変異原学会36回大会・第1回アジア環境変異原学会
  • Place of Presentation: 小倉
• [Presentation] Invovement of MMR in Drosphila somatic cell mutation induced by UV (2007)
  • Author(s): 森恵美子, 平健太郎, 藤井亜世, 有元佐賀惠, 岡本敬の介, 根岸友惠
  • Organizer: 日本環境変異原学会36回大会・第1回アジア環境変異原学会
  • Place of Presentation: 小倉
• [Presentation] Effects of side strea cigarette smoke on the reproducibility of Drosophila exposed at third instar larvae (2007)
  • Author(s): 濱武有子, 森田あゆみ, 岡本敬の介, 有元佐賀惠, 葛西宏, 河井一明, 根岸友惠
  • Organizer: 日本環境変異原学会36回大会・第1回アジア環境変異原学会
  • Place of Presentation: 小倉
• [Presentation] Nucleotide incorporation against 7,8-dihydro-8-oxoguanine is influenced y neighboring base sequences in TLS DNA polymerase reaction (2007)
  • Author(s): Chin-Wei Yung, 他
  • Organizer: 5th international Symposiumu on Nucleic Acids Chemistry
  • Place of Presentation: 東京
• [Presentation] Antimutagenic components in the edible mushroom Agrocybe cylindracea (2007)
  • Author(s): Tomoe Negishi, 他
  • Organizer: 9th International Conference on Mechanisms of Antimutagenesis and Anticarcinogenesis
  • Place of Presentation: 韓国・済州島
• [Presentation] Effects of side stream cigarette smoke on the reproducibility of Drosophila exposed at third instar larvae (2007)
  • Author(s): 濱武 有子, 他
  • Organizer: 日本環境変異原学会36回大会・第1回アジア環境変異原学会
  • Place of Presentation: 小倉
• [Presentation] Antimutagenic components in the edible mushroom Agrocybe cylindrasea (2007)
  • Author(s): 米田 香仁, 他
  • Organizer: 日本環境変異原学会36回大会・第1回アジア環境変異原学会
  • Place of Presentation: 小倉
• [Book] in "Chemoprevention of Cancer and DNA Damage", eds. by Knasmueller et al. (2009)
  • Author(s): Hayatsu, H., Negishi T., Arimoto-Kobayashi, S.
  • Publisher: Chlorophyll against cancer
• [Book] Chemoprevention of Cancer and DNA Damage (2009)
  • Author(s): Hayatsu, H., Negishi T., Arimoto-Kobayashi, S.
  • Total Pages: 9
  • Publisher: Wiley-VCH