Detoxification of azo dye waste by Microorganisms

• Project/Area Number: 19510096
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Niihama National College of Technology
• Principal Investigator: HAYASE Nobuki Niihama National College of Technology, 生物応用化学科, 教授 (00311100)
• Co-Investigator(Kenkyū-buntansha): NAKAGAWA Katsuhiko 新居浜工業高等専門学校, 生物応用化学科, 教授 (40149977) ; USHIO Kazutoshi 新居浜工業高等専門学校, 生物応用化学科, 教授 (30203508)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2009: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2007: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: アゾ染料 / 微生物分解 / 芳香族アミン / 芳香族アミン化合物

Research Abstract

Most of aromatic amines resulting from the cleavage of azo dyes are potentially carcinogenic. Therefore aromatic amines are should be treated and detoxified completely. The main objective of this study is to observe microbial decolorization and detoxification of azo dyes. 1) Bradyrhizobium sp. No.168, which degraded sulfanilic acid resulting from the cleavage of azo bond of Orange II, was isolated. The combination of strain No.168 and azo dyes decolorizing microorganisms decolorized Orange II and degraded sulfanilic acid resulting from the decolorization of Orange II. The continuous decolorization of Orange II and degradation of sulfanilic acid was accomplished by immobilized these microorganisms. 2) 4-amino-1-naphtalenesulfonate resulting from the decolorization of Bordeaux S was degraded by laccase produced from white-rot fungi. The continuous degradation of 4-amino-1-naphtalenesulfonat was accomplished by immobilized laccase on alumina beads.

Research Products

• [Journal Article] 脱窒細菌ma-1株による合成染料の脱色 (2008)
  • Author(s): 早瀬伸樹、伊藤真希、牛尾一利、中川克彦
  • Journal Title: 新居浜工業高等専門学校紀要 44 Pages: 31-36
  • NAID: 40016199513
• [Journal Article] 脱窒細菌MA-1株による合成染料の脱色 (2008)
  • Author(s): 早瀬伸樹
  • Journal Title: 新居浜工業高等専門学校紀要 44 Pages: 31-36
  • NAID: 40016199513
• [Presentation] アゾ染料の微生物分解に関する研究(第2報) (2010)
  • Author(s): 八木裕悟、牛尾一利、中川克彦、早瀬伸樹
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-03-30
• [Presentation] 固定化ラッカーゼによる芳香族アミン化合物の分解 (2010)
  • Author(s): 中川祐登、堤主計、中川克彦、早瀬伸樹
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-03-30
• [Presentation] アゾ染料の微生物分解に関する研究 (2009)
  • Author(s): 八木裕悟、山崎蕗、牛尾一利、中川克彦, 早瀬伸樹
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 2009-03-28
• [Presentation] 白色腐朽菌が生産する酵素による芳香族アミン化合物の分解 (2009)
  • Author(s): 中川祐登、堤主計、中川克彦、早瀬伸樹
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 2009-03-28
• [Presentation] 微生物によるアゾ染料分解 (2009)
  • Author(s): 八木裕悟、山崎蕗、牛尾一利、中川克彦、早瀬伸樹
  • Organizer: 第14回高専シンポジウム
  • Place of Presentation: 高知市文化プラザ
  • Year and Date: 2009-01-24
• [Presentation] 白色腐朽菌が生産する酵素の担体への固定化 (2009)
  • Author(s): 中川祐登、早瀬伸樹、堤主計、中川克彦
  • Organizer: 第14回高専シンポジウム
  • Place of Presentation: 高知市文化プラザ
  • Year and Date: 2009-01-24
• [Presentation] 微生物によるアソ染料分解 (2009)
  • Author(s): 八木裕悟, 山崎蕗, 牛尾一利, 中川克彦, 早瀬伸樹
  • Organizer: 第14回高専シンポジウム
  • Place of Presentation: 高知市文化プラザ
  • Year and Date: 2009-01-24