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Continuous production of virus-like particles by recombinant insect cells

Research Project

Project/Area Number 19560777
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionKobe University

Principal Investigator

YAMAJI Hideki  Kobe University, 大学院工学研究科, 准教授 (40283874)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords昆虫細胞 / 組換えタンパク質生産 / ウイルスタンパク質 / ワクチン / ウイルス様粒子 / 日本脳炎ウイルス / 遺伝子組換え
Research Abstract

ウイルスの表面タンパク質の遺伝子を発現する組換え昆虫細胞を作製し, 安全かつ有効なワクチンとして利用可能なウイルス様粒子の生産について検討した. カイコガ由来のアクチンプロモーターの上流にバキュロウイルス由来のトランス作用因子とエンハンサーを配し, 選択マーカーを有する発現ベクターに, 日本脳炎ウイルスの表面タンパク質prMおよびEの遺伝子を挿入した. このプラスミドを培養昆虫細胞に導入して薬剤存在下で培養することにより, 哺乳動物細胞と比べて100倍以上のEタンパク質を分泌生産する細胞を得ることに成功した.

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report

Research Products

(12 results)

All 2009 2008 2007

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (9 results)

  • [Journal Article] A high-level expression vector containing selectable marker for continuous production of recombinant protein in insect cells2009

    • Author(s)
      Takuya Shishido, Naoya Kurata, Myung Eui Yoon, Tsutomu Tanaka, Hideki Yamaji, Hideki Fukuda, Akihiko Kondo
    • Journal Title

      Biotechnol. Lett. 31

      Pages: 623-627

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production of functional antibody Fab fragment by recombinant insect cells2008

    • Author(s)
      Hideki Yamaji, Toshitaka Manabe, Keizo Watakabe, Masaru Muraoka, Ikuo Fujii, Hideki Fukuda
    • Journal Title

      Biochem. Eng. J. 41

      Pages: 203-209

    • NAID

      120001478904

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production of bionanocapsules in immobilized insect cell culture using porous biomass support particles2007

    • Author(s)
      Takuya Shishido, Masaru Muraoka, Hideki Yamaji, Akihiko Kondo, Hideki Fukuda
    • Journal Title

      J. Biosci. Bioeng. 103

      Pages: 572-574

    • NAID

      110006385794

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Presentation] 昆虫細胞を用いた日本脳炎ウイルスタンパク質の生産2009

    • Author(s)
      高橋裕輔, 中村匡崇, 勝田知尚, 山地秀樹
    • Organizer
      化学工学会第74年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-03-19
    • Related Report
      2008 Final Research Report
  • [Presentation] 昆虫細胞を用いた日木脳炎ウイルスタンパク質の生産2009

    • Author(s)
      高橋 裕輔, 他
    • Organizer
      化学工学会第74年会
    • Place of Presentation
      横浜国立大学
    • Year and Date
      2009-03-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] Low multiplicity infection of 293 cells for adenovirus vector production2008

    • Author(s)
      Naoya Morishita, Kentaro Yamada, Shuji Kubo, Akinobu Gotoh, Tomohisa Katsuda, Hideki Fukuda, Hideki Yamaji
    • Organizer
      JAACT 2008 Fukuoka (The 21th Annual and International Meeting of the Japanese Association for Animal Cell Technology), "POWER OF CELLS, Animal Cell Technology in Bioscience, Bioengineering and Bioindustries"
    • Place of Presentation
      Fukuoka
    • Year and Date
      2008-11-26
    • Related Report
      2008 Final Research Report
  • [Presentation] Production of adenovirus vector by 293 cells immobilized within porous biomass support particles2008

    • Author(s)
      Naoya Morishita, Shuji Kubo, Akinobu Gotoh, Tomohisa Katsuda, Hideki Fukuda, Hideki Yamaji
    • Organizer
      JAACT 2008 Fukuoka (The 21th Annual and International Meeting of the Japanese Association for Animal Cell Technology), "POWER OF CELLS Animal, Cell Technology in Bioscience, Bioengineering and Bioindustries"
    • Place of Presentation
      Fukuoka
    • Year and Date
      2008-11-26
    • Related Report
      2008 Final Research Report
  • [Presentation] Secretory production of Japanese encephalitis virus E protein by recombinant insect cells2008

    • Author(s)
      Yusuke Takahashi, Masataka Nakamura, Takanori Furuta, Tomohisa Katsuda, Hideki Yamaji
    • Organizer
      "Bioseparation for Biorecognition and Bionanotechnology" Conference
    • Place of Presentation
      Hanyang University, Korea
    • Year and Date
      2008-10-27
    • Related Report
      2008 Final Research Report
  • [Presentation] Secretory produclion of Japanese cnccphalilis virus E protcin by recombinant insect cells2008

    • Author(s)
      Y. Takahashi, et al.
    • Organizer
      “Bioseparation for Biorccognition and Bionanotech-nology" Conference
    • Place of Presentation
      Hanyang University, Ansan, Korea
    • Year and Date
      2008-10-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 293細胞の固定化培養によるアデノウイルスベクターの生産2008

    • Author(s)
      森下直矢, 山田健太郎, 山地秀樹, 福田秀樹
    • Organizer
      化学工学会第73年会
    • Place of Presentation
      浜松
    • Year and Date
      2008-03-18
    • Related Report
      2008 Final Research Report
  • [Presentation] Display of functional antibody Fab fragment on the baculovirus surface2007

    • Author(s)
      Hideki Yamaji, Hideki Fukuda
    • Organizer
      13th European Congress on Biotechnology
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2007-09-18
    • Related Report
      2008 Final Research Report
  • [Presentation] 昆虫細胞を用いる機能性抗体の生産システム2007

    • Author(s)
      古田貴憲, 山地秀樹, 福田秀樹
    • Organizer
      化学工学会第39回秋季大会
    • Place of Presentation
      札幌
    • Year and Date
      2007-09-14
    • Related Report
      2008 Final Research Report

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Published: 2007-03-31   Modified: 2016-04-21  

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