• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Evolution of photosynthetic organisms in the Great Oxidation Event

Research Project

Project/Area Number 19570036
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionNagoya University

Principal Investigator

FUJITA Yuichi  Nagoya University, 大学院・生命農学研究科, 准教授 (80222264)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords光合成 / クロロフィル / ラン藻 / アナロガス酵素 / アイソザイム / 進化 / 大酸化イベント / 金属酵素 / クロロフィル生合成 / ラン藻(シアノバクテリア) / コプロポルフィリノーゲンIII酸化酵素 / オキシゲナーゼ / radical SAM / 代謝系の進化 / 嫌気的環境 / イソサイクリック環 / ヒドラターゼ / ラジカルSAM / プロトクロロフィリド
Research Abstract

ラン藻Synechocystis sp. PCC 6803のテトラピロール生合成系のうち4つの酵素系において、環境の酸素レベルに応じて機能分化している複数の系が機能していることを明らかにした。また、その複数の酵素系の成り立ちは、1)構造の異なる酵素系(アナロガス酵素)が利用される場合、3)構造が類似した酵素系(アイソザイム)が利用される場合、という2つに分類できることが判った。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report

Research Products

(55 results)

All 2009 2008 2007

All Journal Article Presentation Book

  • [Journal Article] Prolamellar bodies formed by cyanobacterial protochlorophyllide oxidoreductase in Arabidopsis2009

    • Author(s)
      Masuda, S., Ikeda, R., Masuda, T., Hashimoto, H., Tsuchiya, T., Kojima, H., Nomata, J., Fujita, Y., Mimuro, M., Ohta, H. and Takamiya, K.
    • Journal Title

      Plant J in press

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation and evolution of chlorophyll metabolism2008

    • Author(s)
      Masuda, T. and Fujita, Y.
    • Journal Title

      Photochem. Photobiol. Sci 7

      Pages: 1131-1149

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional expression of nitrogenaselike protochlorophyllide reductase from Rhodobacter capsulatus in Escherichia coli2008

    • Author(s)
      Yamamoto, H., Nomata, J. and Fujita, Y.
    • Journal Title

      Photochem. Photobiol. Sci 7

      Pages: 1238-1242

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] NB-protein (BchNBchB) of dark-operative protochlorophyllide reductase is the catalytic component containing oxygen-tolerant Fe-S clusters2008

    • Author(s)
      Nomata, J., Ogawa, T., Kitashima, M., Inoue, K. and Fujita, Y.
    • Journal Title

      FEBS Lett 582

      Pages: 1346-1350

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of two homologous genes, chlA_I and chlA_<II>, that are differentially involved in isocyclic ring formation of chlorophyll a in the cyanobacterium Synechocystis sp PCC 68032008

    • Author(s)
      Minamizaki, K., Mizoguchi, T., Goto, T., Tamiaki, H. and Fujita, Y.
    • Journal Title

      J. Biol. Chem 283

      Pages: 2684-2692

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation and evolution of chlorophyll metabolism.2008

    • Author(s)
      Masuda, T., Fujita, Y.
    • Journal Title

      Photochemical Photobioloeical Sciences 7

      Pages: 1131-1149

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional expression of nitrogenase-like protochlorophyllide reductase from Rhodobacter capsulatus in Escherichia coli.2008

    • Author(s)
      Yamamoto, H., Nomata, J., Fujita, Y.
    • Journal Title

      Photochemical Photobiological Sciences 7

      Pages: 1238-1242

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of two homologous genes,chlA_I and chlA_<II>, that are differentially involved in isocyclic ring formation of chlorophyll a in the cyanobacterium Synechocystis sp. PCC 68032008

    • Author(s)
      K. Minamizaki, T. Mizoguchi, T. Goto, H. Tamiaki and Y.Fujita
    • Journal Title

      Journal of Biological Chemistry 283巻

      Pages: 2684-2692

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NB-protein (BchN-BchB) of dark-operative protochlorophyllide reductase is the catalytic component containing oxygen-tolerant Fe-S clusters2008

    • Author(s)
      J. Nomata, T. Ogawa, M. Kitashima, K. lnone and Y. Fujita
    • Journal Title

      FEBS Letters 582巻

      Pages: 1346-1350

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] ニトロゲナーゼ類似型プロトクロロフィリド還元酵素の触媒コンポーネントNB-蛋白質の立体構造に基づく立体特異的なD環二重結合還元機構2009

    • Author(s)
      野亦次郎・張本純平・村木則文・溝口正・民秋均・栗栖源嗣・藤田祐一
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-23
    • Related Report
      2008 Final Research Report
  • [Presentation] ラン藻Leptolyngbya boryanaにおけるプロトクロロフィリド還元酵素の酸素感受性2009

    • Author(s)
      山本治樹・久留宮祥平・大橋理恵・藤田祐一
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-23
    • Related Report
      2008 Final Research Report
  • [Presentation] ラン藻Synechocystis sp. PCC6803のヘム・クロロフィル生合成系における2つのコプロポルフィリノーゲンIIIオキシダーゼ2009

    • Author(s)
      後藤武知・藤田祐一
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-23
    • Related Report
      2008 Final Research Report
  • [Presentation] ラン藻Synechocystis sp. PCC 6803 のヘム・クロロフィル生合成系における2つのコプロポフィリノーダンIIIオキ. シダーゼ2009

    • Author(s)
      後藤武知, 藤田祐一
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-03-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] ラン藻Synechocystis sp. PCC 6803 におけるクロロフィルのE環生成に関わる2つのアイソフォームChlA_1とChlA_<II>の互換性2009

    • Author(s)
      川尻安志, 南崎啓, 藤田祐一
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-03-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] ラン藻Synechocystis sp. PCC 6803 における2つのヘムオキシゲナーゼアイソフォームの機能分化2009

    • Author(s)
      青木里奈, 後藤武知, 藤田祐一
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-03-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] ラン藻Synechocystis sp. PCC6803における2つのヘムオキシゲナーゼアイソフォームの機能分化2009

    • Author(s)
      青木里奈・後藤武知・南崎啓・藤田祐一
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] ラン藻Synechocystis sp. PCC6803におけるクロロフィルのE環生成に関わる2つのアイソフォームChlA_I とChlA_<II>の互換性2009

    • Author(s)
      川尻安志・南崎啓・藤田祐一
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] ラン藻Leptolyngbya boryanaにおける光依存型プロトクロロフィリド還元酵素の大量発現2009

    • Author(s)
      小島寛子・大城香・藤田祐一
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] Structural aspects of dark-operative protochlorophyllide reductase2009

    • Author(s)
      Fujita, Y.
    • Organizer
      International Symposium on Chemistry of Reductases II
    • Place of Presentation
      Nagoya, Japan (Invited Speaker)
    • Related Report
      2008 Final Research Report
  • [Presentation] クロロフィル生合成系の酸素環境に対する適応進化2008

    • Author(s)
      藤田祐一
    • Organizer
      日本植物学会第72回大会シンポジウム「テトラピロール代謝調節、進化と多機能性」
    • Place of Presentation
      高知大学
    • Year and Date
      2008-09-25
    • Related Report
      2008 Final Research Report
  • [Presentation] クロロフィル生合成系の酸素環境に対する適応進化2008

    • Author(s)
      藤田祐一
    • Organizer
      日本植物学会第72回大会シンポジウム
    • Place of Presentation
      高知大学
    • Year and Date
      2008-09-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] 光非依存型(暗所作動型)プロトクロロフィリド還元酵素のX線構造解析2008

    • Author(s)
      村木則文・野亦次郎・志波智生・藤田祐一・栗栖源嗣
    • Organizer
      第49回日本植物生理学会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-22
    • Related Report
      2008 Final Research Report
  • [Presentation] ニトロゲナーゼ類似型プロトクロロフィリド還元酵素の触媒コンポーネントNB-蛋白質の金属中心の解析2008

    • Author(s)
      野亦次郎・江端梢・村木則文・栗栖源嗣・藤田祐一
    • Organizer
      第49回日本植物生理学会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-22
    • Related Report
      2008 Final Research Report
  • [Presentation] ラン藻Leptolyngbya boryana(Plectonema boryanum)のプロトクロロフィリド還元酵素の触媒コンポーネントNB-蛋白質(ChlN-ChlB)の生化学的解析2008

    • Author(s)
      山本治樹・久留宮祥平・大橋理恵・藤田祐一
    • Organizer
      第49回日本植物生理学会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-22
    • Related Report
      2008 Final Research Report
  • [Presentation] ラン藻Synechocystis sp. PCC6803のヘム・クロロフィリド生合成系における2つのコプロポルフィリノーゲンIIIオキシダーゼ2008

    • Author(s)
      後藤武知・南崎啓・藤田祐一
    • Organizer
      第49回日本植物生理学会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-22
    • Related Report
      2008 Final Research Report
  • [Presentation] 光非依存型(暗所作動型)プロトクロロフィリド還元酵素のX線構造解2008

    • Author(s)
      村木則文・野亦次郎・志波智生・藤田祐一・栗栖源嗣
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌(札幌コンベンションセンター)
    • Year and Date
      2008-03-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] ニトロゲナーゼ類似型プロトクロロフィリド還元酵素の触媒コンポーネントNB-蛋白質の金属中心の解析2008

    • Author(s)
      野亦次郎・江端梢・村木則文・栗栖源嗣・藤田祐一
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌(札幌コンベンションセンター)
    • Year and Date
      2008-03-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] ラン藻Leptolyngbya boryana (Plectonema boryanum)のプロトクロロフィリド還元酵素の触媒コンポーネントNB-蛋白質(ChiN-ChiB)の生化学的解析2008

    • Author(s)
      山本治樹・久留宮祥平・大橋理恵・藤田祐一
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌(札幌コンベンションセンダー)
    • Year and Date
      2008-03-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] Characterization of nitrogenase-like enzymes in chlorophyll and bacteriochlorophyll biosynthesis2008

    • Author(s)
      Y. Fujita
    • Organizer
      lnternational Symposium on Chemistry of Reductases
    • Place of Presentation
      名古屋(名古屋大学)
    • Year and Date
      2008-03-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] Nitrogenase homologues in chlorophyll biosynthesis2008

    • Author(s)
      Fujita, Y
    • Organizer
      Gordon Research Conference on Iron-Sulfur Enzymes
    • Place of Presentation
      Colby Sawyer College, New London, NH, USA (Invited Speaker)
    • Related Report
      2008 Final Research Report
  • [Presentation] 光非依存型(暗所作動型)プロトクロロフィリド還元酵素の解析2008

    • Author(s)
      野亦次郎・江端梢・村木則文・志波智生・栗栖源嗣・藤田祐一
    • Organizer
      第8回光合成研究会公開シンポジウム「光合成を支える多様な分子システム」
    • Place of Presentation
      名古屋大学
    • Related Report
      2008 Final Research Report
  • [Presentation] ラン藻Leptolygbya boryanaにおける暗所作動型プロトクロロフィリド還元酵素の生化学的解析2008

    • Author(s)
      山本治樹・久留宮祥平・大橋理恵・藤田祐一
    • Organizer
      第8回光合成研究会公開シンポジウム「光合成を支える多様な分子システム」
    • Place of Presentation
      名古屋大学
    • Related Report
      2008 Final Research Report
  • [Presentation] Characterization of nitrogenase-like enzymes in chlorophyll and bacteriochlorophyll biosynthesis pathways2008

    • Author(s)
      Fujita, Y.
    • Organizer
      International Symposium on Chemistry of Reductases
    • Place of Presentation
      Nagoya, Japan (Invited Speaker)
    • Related Report
      2008 Final Research Report
  • [Presentation] Comparative analysis of two nitrogenase-like enzymes, protochlorophyllide reductase and chlorophyllide a reductase2008

    • Author(s)
      J. Nomata, M. Kitashima, T. Ogawa, K. lnoue, Y. Fujita
    • Organizer
      lnternational Symposium on Chemistry of Reductases
    • Place of Presentation
      名古屋(名古屋大学)
    • Related Report
      2007 Annual Research Report
  • [Presentation] ラン藻Synechocystis sp.PCC 6803のヘム・クロロフィル生命成系における2つのコプロポルフィリノーゲンIIIオキシダーゼ2008

    • Author(s)
      後藤武知・南崎啓・藤田祐一
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌(札幌コンベンションセンター)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Final two stages of chlorophyllide a formation in chlorophyll biosynthesis: E-ring formation and D-ring reduction2007

    • Author(s)
      Y. Fujita
    • Organizer
      lnternational Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms
    • Place of Presentation
      京都(京都テルサ)
    • Year and Date
      2007-12-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] ラン藻Synechocystis sp. PCC6803におけるクロロフィルのE環生成反応2007

    • Author(s)
      藤田祐一
    • Organizer
      かずさDNA研研究会ラン藻の分子生物学2007
    • Place of Presentation
      かずさDNA研
    • Year and Date
      2007-12-04
    • Related Report
      2008 Final Research Report
  • [Presentation] ラン藻Synechocystis sp. PCC 6803におけるクロロフィルE環生成反応2007

    • Author(s)
      藤田祐一
    • Organizer
      かずさDNA研究所研究会「ラン藻の分子生物学2007」
    • Place of Presentation
      木更津(かずさアーク)
    • Year and Date
      2007-12-04
    • Related Report
      2007 Annual Research Report
  • [Presentation] クロロフィル生合成系におけるニトロゲナーゼ類似酵素の阻害剤2007

    • Author(s)
      野亦次郎・溝口正・民秋均・藤田祐一
    • Organizer
      第71回日本生化学会中部例会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2007-05-31
    • Related Report
      2008 Final Research Report
  • [Presentation] Final two stages of chlorophyllide a formation in chlorophyll biosynthesis : E-ring formation and D-ring reduction2007

    • Author(s)
      Fujita, Y.
    • Organizer
      7th International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms
    • Place of Presentation
      Kyoto, Japan (Invited Speaker)
    • Related Report
      2008 Final Research Report
  • [Presentation] ラン藻Synechocystis sp. PCC6803のクロロフィルのE環は2つの酸素依存型酵素によって生成される2007

    • Author(s)
      南崎啓・後藤武知・藤田祐一
    • Organizer
      光合成研究会シンポジウム
    • Place of Presentation
      岡山大学
    • Related Report
      2008 Final Research Report
  • [Presentation] Characterization of metal center of the catalytic components, NB-protein, of dark-operative protochlorophyllide reductase from Rhodobacter capsulatus2007

    • Author(s)
      J. Nomata, K. Ebata, T. Ogawa, K. lnoue, Y. Fujita
    • Organizer
      lnternational Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms
    • Place of Presentation
      京都(京都テルサ)
    • Related Report
      2007 Annual Research Report
  • [Presentation] The catalytic component NB-protein (ChlN-ChlB) of protochlorophyllide reductase from the cyanobacterium Leptolyngbya boryana2007

    • Author(s)
      H.Yamamoto, S. Kurumiya, R. Ohashi, Y. Fujita
    • Organizer
      lnternational Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms
    • Place of Presentation
      京都(京都テルサ)
    • Related Report
      2007 Annual Research Report
  • [Presentation] ldentification of two acsF genes involved in isocyclic ring formation of chlorophylla in the cyanobacterium Synechocystis sp. PCC 68032007

    • Author(s)
      K. Minamizaki, T. Goto, T. Mizoguchi, H. Tamiaki, Y. Fujita
    • Organizer
      lnternational Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms
    • Place of Presentation
      京都(京都テルサ)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Two homologous genes, chlAl and chlAll, are differentially involved in isocyclic ring formation of chlorophyll a in the cyanobacterium Synechocystis sp. PCC 68032007

    • Author(s)
      K. Minamizaki, T. Goto, T. Mizoguchi, H. Tamiaki, Y. Fujita
    • Organizer
      lnternational Symposium on Biosynthesis of Tetrapyrroles
    • Place of Presentation
      草津(立命館大学)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Chlorophyll a biosynthesis under anaerobic environments in the cyanobacterium Synechocystis sp. PCC 68032007

    • Author(s)
      T. Goto, K. Minamizaki, Y. Fujita
    • Organizer
      14th lnternational Congress of Photosynthesis
    • Place of Presentation
      Glasgow
    • Related Report
      2007 Annual Research Report
  • [Presentation] Biochemical analysis of two catalytic components of nitrogenase-like enzymes protochlorophyllide reductase and chlorophyllide a reductase from Rhodobacter capsulatus2007

    • Author(s)
      J. Nomata, M. Kitashima, T. Ogawa, K. lnoue, Y. Fujita
    • Organizer
      14th lnternational Congress of Photosynthesis
    • Place of Presentation
      Glasgow
    • Related Report
      2007 Annual Research Report
  • [Presentation] ラン藻Synechocystis sp. PCC 6803のクロロフィルのE環は2つの酸素依存型酵素によって生成される2007

    • Author(s)
      南崎啓・後藤武知・藤田祐一
    • Organizer
      光合成研究会シンポジウム
    • Place of Presentation
      岡山(岡山大学)
    • Related Report
      2007 Annual Research Report
  • [Book] 光合成細菌Rhodobacter capsulatus における大量発現と形質転換「光合成研究法」低温科学67 巻2009

    • Author(s)
      藤田祐一、野亦次郎
    • Publisher
      北海道大学低温科学研究所
    • Related Report
      2008 Final Research Report
  • [Book] 嫌気条件下でのタンパク質精製「光合成研究法」低温科学67 巻2009

    • Author(s)
      藤田祐一、野亦次郎
    • Publisher
      北海道大学低温科学研究所
    • Related Report
      2008 Final Research Report
  • [Book] 低温科学第67巻「光合成研究法」2009

    • Author(s)
      藤田祐一, 野亦次郎
    • Total Pages
      5
    • Publisher
      嫌気条件下でのタンパク質精製
    • Related Report
      2008 Annual Research Report
  • [Book] Chlorophyll a biosynthesis under anaerobic environments in the cyanobacterium Synechocystis sp. PCC 6803. In Photosynthesis. Energy from the Sun: 14th International Congress on Photosynthesis2008

    • Author(s)
      Minamizaki, K., Goto, T. and Fujita, Y.
    • Publisher
      Edited by Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B., Springer
    • Related Report
      2008 Final Research Report
  • [Book] Biochemical analysis of two catalytic components of nitrogenaselike enzymes protochlorophyllide reductase and chlorophyllide a reductase from Rhodobacter capsulatus. In Photosynthesis. Energy from the Sun: 14th International Congress on Photosynthesis2008

    • Author(s)
      Nomata, J., Kitashima, M., Ogawa, T., Inoue, K and Fujita Y.
    • Publisher
      Edited by Allen, J. F., Gantt, E., Golbeck, J. H., Osmond, B., Springer
    • Related Report
      2008 Final Research Report
  • [Book] Photosyntheis. Energy from the Sun: 14th International Congress on Photosynthesis2008

    • Author(s)
      K. Minamizaki, T. Goto, Y. Fujita
    • Total Pages
      4
    • Publisher
      Chlorophyll a biosynthesis under anaerobic environments in the cyanobacterium Synechocystis sp. PCC 6803
    • Related Report
      2007 Annual Research Report
  • [Book] Photosyntheis. Energy from the Sun: 14th International Congress on Photosynthesis2008

    • Author(s)
      J. Nomata, M. Kitashima, T. Ogawa, K. Inoue, Y. Fujita
    • Total Pages
      4
    • Publisher
      Biochemical analysis of two catalytic components of nitrogenase-like enzymes protochlorophyllide reductase and chlorophyllide a reductase from Rhodobacter capsulatus
    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi