• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Construction of recombinant gonadotropin and establishment of the artificial fertilization system in hagfish.

Research Project

Project/Area Number 19570056
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionNiigata University

Principal Investigator

UCHIDA Katsuhisa  Niigata University, 自然科学系, 助教 (50360508)

Co-Investigator(Kenkyū-buntansha) MASUMI Nozaki  新潟大学, 自然科学系, 教授 (70136232)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords比較内分泌学 / 生理学 / 生殖 / 進化 / ホルモン / 比較内分泌 / 発生・分化 / 生体生命情報学
Research Abstract

下垂体から分泌され、生殖腺を刺激するホルモン(ゴナドトロピン;GTH)は、顎を持つ脊椎動物では、性ステロイドを介した配偶子の形成・成熟や、繁殖行動の誘起に必須の役割を担っている。本研究では、現存する最古の脊椎動物であるヌタ(メクラ)ウナギの下垂体GTH遺伝子の同定を発端に、酵母細胞を宿主として組換え型GTHを遺伝子工学的に構築している。この組換え型ホルモンは、ヌタウナギ自身が持つ天然型GTHと生化学的に極めて類似した構造を持ち、生殖腺からの性ステロイドの分泌を促進することが示唆された。

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (40 results)

All 2010 2009 2008 2007

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (26 results)

  • [Journal Article] Changes in genexpression levels of somatolactin in the pituitary and morphology of gill mitochondria-rich cells in Mozambique tilapia after transfer to acidic freshwater (pH 3. 5)2010

    • Author(s)
      Furukawa F, Watanabe S, Kaneko T, Uchida K
    • Journal Title

      General and Comparative Endocrinology Vol.166

      Pages: 549-555

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Changes in gene expression levels of somatolaction the pituitary and morphology of gill mitochondria-rich cells in Mozambique tilapia after transfer to acidic freshwater (pH 3.5).2010

    • Author(s)
      Furukawa F, Watanabe S, Kane Ko T, Uchida K.
    • Journal Title

      General and Comparative Endocrinology (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA cloning and isolation of somatolactin in Mozambique tilapia, and effects of seawater acclimation, confinement stress, fasting on its pituitary expression2009

    • Author(s)
      Uchida K., Moriyama S., Breves J.P., Fox B.K., 他4名
    • Journal Title

      General and Comparative Endocrinology Vol.161

      Pages: 162-170

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The hagfish pituitary gland and its putative adenohypophysialhormones2008

    • Author(s)
      Nozaki M
    • Journal Title

      Zoological Science Vol.25

      Pages: 1028-1036

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identity and distribution of immunoreactive adenohypophysial cells in the pituitary during the life cycle of sea lampreys, Petromyzon marinus2008

    • Author(s)
      Nozaki M., Ominato K., Shimotani T., Kawauchi H., Youson J.H., Sower S. A
    • Journal Title

      General and Comparative Endocrinology Vol.155

      Pages: 403-412

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Origins of gonadotropin-releasing hormone (GnRH) in vertebrates: Identification of a novel GnRH in a basal vertebrates, the sea lamprey2008

    • Author(s)
      Kavanaugh S.I., Nozaki M., Sower S. A
    • Journal Title

      Endocrinology Vol.149

      Pages: 3860-3869

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene structure and functional characterization of growth hormone in dogfish, Squalus acanthias2008

    • Author(s)
      Moriyama S., Oda M., Tamazaki T., Yamaguchi K., Amiya N., Takahashi A., Amano M., Goto T., Nozaki M., 他2名
    • Journal Title

      Zoological Science Vol.25

      Pages: 604-613

    • NAID

      40016249905

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The hagfish pituitary gland and its putative adenohypophysialhormones2008

    • Author(s)
      Nozaki M
    • Journal Title

      Zoological Science 25

      Pages: 1028-1036

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identity and distribution of immunoreactive adenohypophysial cells in the pituitary during the life cycle of sea lampreys, Petromyzon marinus2008

    • Author(s)
      Nozaki M., Ominato K., Shimotani T., Kawauchi H., Youson J.H., Sower S.A.
    • Journal Title

      General and Comparative Endocrinology 155

      Pages: 403-412

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Origins of gonadotropin-releasing hormone(GnRH)in vertebrates : Identification of a novel GnRH in a basal vertebrates, the sea lamprey2008

    • Author(s)
      Kavanaugh S.I., Nozaki M., Sower S.A.
    • Journal Title

      Endocrinology 149

      Pages: 3860-3869

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene structure and functional characterization of growth hormone in dogfish, Squalus acanthias2008

    • Author(s)
      Moriyama S., Oda M., Tamazaki T., Yamaguchi K., Amiya N., Takahashi A., Amano M., Goto T., Nozaki M., 他2名
    • Journal Title

      Zoological Science 25

      Pages: 604-613

    • NAID

      40016249905

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] cDNA cloning and isolation of somatolactin in Mozambique tilapia, and effects of seawater acclimation, confinement stress fasting on its pituitary expression2008

    • Author(s)
      Uchida K., Moriyama S., Breves J.P., Fox B.K., 他4名
    • Journal Title

      General and Comparative Endocrinology 25

      Pages: 604-613

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gonadotropin-like and adrenocorticotropin-like cells in the pituitary gland of hagfish, Paramyxine atami: immunohistochemistry in combination with lectin histochemistry2007

    • Author(s)
      Nozaki M., Shimotani T., Uchida K
    • Journal Title

      Cell and Tissue Research Vol.328

      Pages: 563-572

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gonadotropin-like and adrenocorticotropin-like cells in the pituitary gland of hagfish, Paramyxine atami: immunohistochemistry in combination with lectin histochemistry.2007

    • Author(s)
      Nozaki M., Shimotani T. and Uchida K.
    • Journal Title

      Cell and Tissue Research 328

      Pages: 563-572

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Effects of estradiol and testosterone treatment on GTH and ACTH cells in the pituitary of the hagfish2010

    • Author(s)
      Nozaki M., Honda K., Miki M., Shimotani T., Uchida K
    • Organizer
      6th Intercongress Symposium of the Asia Oceania Society for Comparative Endocrinology
    • Place of Presentation
      New Zealand
    • Related Report
      2009 Final Research Report
  • [Presentation] Effects of estradiol and testosterone treatment on GTH and ACTH cells in the pituitary of the hagfish.2010

    • Author(s)
      Nozaki M., Honda K., Miki M., Shimotani T., Uchida K.
    • Organizer
      6th Intercongress Symposium of the Asia Oceania Society for Comparative Endocrinology
    • Place of Presentation
      New Zealand
    • Related Report
      2009 Annual Research Report
  • [Presentation] クロヌタウナギにおける組換え生殖腺刺激ホルモンの構築2009

    • Author(s)
      内田勝久、亀井宏泰、森山俊介、野崎眞澄, 他2名
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] クロヌタウナギの腺下垂体細胞に対するエストロジェンとテストステロン投与の影響2009

    • Author(s)
      野崎眞澄、本田香織、三木誠、下谷豊和、内田勝久
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] ヌタウナギの新規RFamideペプチドの同定と生理作用の解析2009

    • Author(s)
      大杉知裕、内田勝久、野崎眞澄、筒井和義
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] ホヤ下垂体研究の現状-マボヤとカタユウレイボヤの比較ー2009

    • Author(s)
      寺門潔、下谷豊和、野崎眞澄
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Identification of hagfish gonadotropin (GTH) and cellular distribution of GTH and adrenocorticotropin (ACTH) in the hagfish adenohypophysis2009

    • Author(s)
      Nozaki M., Uchida K., Miki M., Shimotani T., Honda K., Moriyama, S
    • Organizer
      The 16th International Congress of Comparative Endocrinology
    • Place of Presentation
      Hong Kong
    • Related Report
      2009 Final Research Report
  • [Presentation] Novel hagfish hypothalamic RFamide peptides: isolation, localization and biological activity2009

    • Author(s)
      Osugi T., Uchida K., Nozaki M., Sower S.A., Tsutsui K
    • Organizer
      The 16th International Congress of Comparative Endocrinology
    • Place of Presentation
      Hong Kong
    • Related Report
      2009 Final Research Report
  • [Presentation] Identification of hagfish gonadotropin (GTH) and cellular distribution of GTH and adrenocrticotropin (ACTH) in the hagfish adenohypophysis2009

    • Author(s)
      Nozaki M., Uchida K., Miki M., Shimotani T., Honda K., Moriyama, S.
    • Organizer
      The 16th International Congress of Comparative Endocrinology
    • Place of Presentation
      Hong Kong
    • Related Report
      2009 Annual Research Report
  • [Presentation] Novel hagfish hypothalamic RFamide peptides : isolatiom, localization and biological avtivity.2009

    • Author(s)
      Osugi T., Uchida K., Nozaki M., Sower S.A., Tsutsui K.
    • Organizer
      The 16th International Congress of Comparative Endocrinology
    • Place of Presentation
      Hong Kong
    • Related Report
      2009 Annual Research Report
  • [Presentation] クロヌタウナギにおける生殖腺刺激ホルモンの単離・精製と機能解析2008

    • Author(s)
      内田勝久、森山俊介、千葉洋明、下谷豊和、野崎眞澄
    • Organizer
      日本比較内分泌学会第33回大会
    • Place of Presentation
      広島
    • Year and Date
      2008-12-06
    • Related Report
      2009 Final Research Report
  • [Presentation] クロヌタウナギにおける生殖腺刺激ホルモンの単離・精製と機能解析2008

    • Author(s)
      内田勝久, 他4名
    • Organizer
      日本比較内分泌学会第33回大会
    • Place of Presentation
      広島大学学士会館
    • Year and Date
      2008-12-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] Identity of hagfish gonadotropin (GTH) and cellular distribution of GTH and adrenocorticotropin (ACTH) in the hagfish adenohypophysis2008

    • Author(s)
      Nozaki M., Uchida K., Miki M., Shimotani S., Honda K., Moriyama S
    • Organizer
      The 15th CDB Meeting, "Advances in Cyclostome Research: Body plan and developmental programs before jawed vertebrates
    • Place of Presentation
      Kobe, Japan
    • Year and Date
      2008-01-25
    • Related Report
      2009 Final Research Report
  • [Presentation] Identity of hagfish gonadotropin (GTH) and cellular distribution of GTH and adrenocorticotropin (ACTH) in the hagfish adenohypophysis.2008

    • Author(s)
      Nozaki M., Uchida K., Miki M., Shimotani S., Honda K. and Moriyama S.
    • Organizer
      The 15th CDB Meeting, "Advances in Cyclostome Research: Body plan and developmental programs before jawed vertebrates
    • Place of Presentation
      RIKEN Kobe CDB Institute
    • Year and Date
      2008-01-25
    • Related Report
      2007 Annual Research Report
  • [Presentation] Identification of hagfish gonadotropin (GTH) and its evolutionary implication2007

    • Author(s)
      Nozaki M., Shimotani S., Uchida K., Moriyama S
    • Organizer
      Okazaki Biology Conference, OBC 6 on "Marine Biology"
    • Place of Presentation
      Okazaki, Japan
    • Year and Date
      2007-12-03
    • Related Report
      2009 Final Research Report
  • [Presentation] Identification of hagfish gonadotropin (GTH) and its evolutionary implication.2007

    • Author(s)
      Nozaki M., Shimotani S., Uchida K. and Moriyama S.
    • Organizer
      Okazaki Biology Conference, OBC 6 on "Marine Biology"
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2007-12-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] 最古の脊椎動物・ヌタウナギの生殖腺刺激ホルモン2007

    • Author(s)
      内田勝久
    • Organizer
      平成19年度東京大学海洋研究所共同利用研究集会「魚類の適応と進化の統合生物学:遺伝子から行動まで」
    • Place of Presentation
      東京
    • Year and Date
      2007-11-16
    • Related Report
      2009 Final Research Report
  • [Presentation] 最古の脊椎動物・ヌタウナギの生殖腺刺激ホルモン2007

    • Author(s)
      内田勝久
    • Organizer
      平成19年度東京大学海洋研究所共同利用研究集会「魚類の適応と進化の統合生物学:遺伝子から行動まで」
    • Place of Presentation
      東京大学海洋研究所
    • Year and Date
      2007-11-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] 日本比較内分泌学会第32回大会2007

    • Author(s)
      野崎眞澄、下谷豊和、内田勝久、森山俊介、高橋明義、川内浩司、Stacia A.Sower
    • Organizer
      日本比較内分泌学会第32回大会
    • Place of Presentation
      栃木
    • Year and Date
      2007-10-12
    • Related Report
      2009 Final Research Report
  • [Presentation] 無顎類からみた下垂体と腺下垂体ホルモンの進化2007

    • Author(s)
      野崎眞澄、下谷豊和、内田勝久、森山俊介、高橋明義、川内浩司、Stacia A. Sower
    • Organizer
      日本比較内分泌学会第32回大会
    • Place of Presentation
      栃木県・日光プリンスホテル
    • Year and Date
      2007-10-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] クロヌタウナギの下垂体における生殖腺刺激ホルモン遺伝子発現解析2007

    • Author(s)
      内田勝久、森山俊介、本田香織、下谷豊和、野崎眞澄
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      青森
    • Year and Date
      2007-09-20
    • Related Report
      2009 Final Research Report
  • [Presentation] クロヌタウナギの腺下垂体におけるACTH細胞の同定2007

    • Author(s)
      野崎眞澄、下谷豊和、内田勝久、安田明和
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      青森
    • Year and Date
      2007-09-20
    • Related Report
      2009 Final Research Report
  • [Presentation] クロヌタウナギの下垂体における生殖腺刺激ホルモン遺伝子発現解析2007

    • Author(s)
      内田勝久、森山俊介、本田香織、下谷豊和、野崎眞澄
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前大学
    • Year and Date
      2007-09-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] クロヌタウナギの腺下垂体におけるACTH細胞の同定2007

    • Author(s)
      野崎眞澄、下谷豊和、内田勝久、安田明和
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前大学
    • Year and Date
      2007-09-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] 新潟県産クロヌタウナギの生殖腺刺激ホルモン遺伝子の単離を発端に2007

    • Author(s)
      内田勝久
    • Organizer
      日本下垂体研究会第22回学術集会
    • Place of Presentation
      神奈川
    • Year and Date
      2007-08-25
    • Related Report
      2009 Final Research Report
  • [Presentation] 新潟県産クロヌタウナギの生殖腺刺激ホルモン遺伝子の単離を発端に2007

    • Author(s)
      内田勝久
    • Organizer
      日本下垂体研究会第22回学術集会
    • Place of Presentation
      神奈川県・湘南国際村センター国際会議場
    • Year and Date
      2007-08-25
    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi