Construction of recombinant gonadotropin and establishment of the artificial fertilization system in hagfish.

• Project/Area Number: 19570056
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Niigata University
• Principal Investigator: UCHIDA Katsuhisa Niigata University, 自然科学系, 助教 (50360508)
• Co-Investigator(Kenkyū-buntansha): MASUMI Nozaki 新潟大学, 自然科学系, 教授 (70136232)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 比較内分泌学 / 生理学 / 生殖 / 進化 / ホルモン / 比較内分泌 / 発生・分化 / 生体生命情報学

Research Abstract

下垂体から分泌され、生殖腺を刺激するホルモン(ゴナドトロピン;GTH)は、顎を持つ脊椎動物では、性ステロイドを介した配偶子の形成・成熟や、繁殖行動の誘起に必須の役割を担っている。本研究では、現存する最古の脊椎動物であるヌタ(メクラ)ウナギの下垂体GTH遺伝子の同定を発端に、酵母細胞を宿主として組換え型GTHを遺伝子工学的に構築している。この組換え型ホルモンは、ヌタウナギ自身が持つ天然型GTHと生化学的に極めて類似した構造を持ち、生殖腺からの性ステロイドの分泌を促進することが示唆された。

Research Products

• [Journal Article] Changes in genexpression levels of somatolactin in the pituitary and morphology of gill mitochondria-rich cells in Mozambique tilapia after transfer to acidic freshwater (pH 3. 5) (2010)
  • Author(s): Furukawa F, Watanabe S, Kaneko T, Uchida K
  • Journal Title: General and Comparative Endocrinology Vol.166 Pages: 549-555
  • Peer Reviewed
• [Journal Article] Changes in gene expression levels of somatolaction the pituitary and morphology of gill mitochondria-rich cells in Mozambique tilapia after transfer to acidic freshwater (pH 3.5). (2010)
  • Author(s): Furukawa F, Watanabe S, Kane Ko T, Uchida K.
  • Journal Title: General and Comparative Endocrinology (印刷中)
  • Peer Reviewed
• [Journal Article] DNA cloning and isolation of somatolactin in Mozambique tilapia, and effects of seawater acclimation, confinement stress, fasting on its pituitary expression (2009)
  • Author(s): Uchida K., Moriyama S., Breves J.P., Fox B.K., 他4名
  • Journal Title: General and Comparative Endocrinology Vol.161 Pages: 162-170
  • Peer Reviewed
• [Journal Article] The hagfish pituitary gland and its putative adenohypophysialhormones (2008)
  • Author(s): Nozaki M
  • Journal Title: Zoological Science Vol.25 Pages: 1028-1036
  • Peer Reviewed
• [Journal Article] Identity and distribution of immunoreactive adenohypophysial cells in the pituitary during the life cycle of sea lampreys, Petromyzon marinus (2008)
  • Author(s): Nozaki M., Ominato K., Shimotani T., Kawauchi H., Youson J.H., Sower S. A
  • Journal Title: General and Comparative Endocrinology Vol.155 Pages: 403-412
  • Peer Reviewed
• [Journal Article] Origins of gonadotropin-releasing hormone (GnRH) in vertebrates: Identification of a novel GnRH in a basal vertebrates, the sea lamprey (2008)
  • Author(s): Kavanaugh S.I., Nozaki M., Sower S. A
  • Journal Title: Endocrinology Vol.149 Pages: 3860-3869
  • Peer Reviewed
• [Journal Article] Gene structure and functional characterization of growth hormone in dogfish, Squalus acanthias (2008)
  • Author(s): Moriyama S., Oda M., Tamazaki T., Yamaguchi K., Amiya N., Takahashi A., Amano M., Goto T., Nozaki M., 他2名
  • Journal Title: Zoological Science Vol.25 Pages: 604-613
  • NAID: 40016249905
  • Peer Reviewed
• [Journal Article] The hagfish pituitary gland and its putative adenohypophysialhormones (2008)
  • Author(s): Nozaki M
  • Journal Title: Zoological Science 25 Pages: 1028-1036
  • Peer Reviewed
• [Journal Article] Identity and distribution of immunoreactive adenohypophysial cells in the pituitary during the life cycle of sea lampreys, Petromyzon marinus (2008)
  • Author(s): Nozaki M., Ominato K., Shimotani T., Kawauchi H., Youson J.H., Sower S.A.
  • Journal Title: General and Comparative Endocrinology 155 Pages: 403-412
  • Peer Reviewed
• [Journal Article] Origins of gonadotropin-releasing hormone(GnRH)in vertebrates : Identification of a novel GnRH in a basal vertebrates, the sea lamprey (2008)
  • Author(s): Kavanaugh S.I., Nozaki M., Sower S.A.
  • Journal Title: Endocrinology 149 Pages: 3860-3869
  • Peer Reviewed
• [Journal Article] Gene structure and functional characterization of growth hormone in dogfish, Squalus acanthias (2008)
  • Author(s): Moriyama S., Oda M., Tamazaki T., Yamaguchi K., Amiya N., Takahashi A., Amano M., Goto T., Nozaki M., 他2名
  • Journal Title: Zoological Science 25 Pages: 604-613
  • NAID: 40016249905
  • Peer Reviewed
• [Journal Article] cDNA cloning and isolation of somatolactin in Mozambique tilapia, and effects of seawater acclimation, confinement stress fasting on its pituitary expression (2008)
  • Author(s): Uchida K., Moriyama S., Breves J.P., Fox B.K., 他4名
  • Journal Title: General and Comparative Endocrinology 25 Pages: 604-613
  • Peer Reviewed
• [Journal Article] Gonadotropin-like and adrenocorticotropin-like cells in the pituitary gland of hagfish, Paramyxine atami: immunohistochemistry in combination with lectin histochemistry (2007)
  • Author(s): Nozaki M., Shimotani T., Uchida K
  • Journal Title: Cell and Tissue Research Vol.328 Pages: 563-572
  • Peer Reviewed
• [Journal Article] Gonadotropin-like and adrenocorticotropin-like cells in the pituitary gland of hagfish, Paramyxine atami: immunohistochemistry in combination with lectin histochemistry. (2007)
  • Author(s): Nozaki M., Shimotani T. and Uchida K.
  • Journal Title: Cell and Tissue Research 328 Pages: 563-572
  • Peer Reviewed
• [Presentation] Effects of estradiol and testosterone treatment on GTH and ACTH cells in the pituitary of the hagfish (2010)
  • Author(s): Nozaki M., Honda K., Miki M., Shimotani T., Uchida K
  • Organizer: 6th Intercongress Symposium of the Asia Oceania Society for Comparative Endocrinology
  • Place of Presentation: New Zealand
• [Presentation] Effects of estradiol and testosterone treatment on GTH and ACTH cells in the pituitary of the hagfish. (2010)
  • Author(s): Nozaki M., Honda K., Miki M., Shimotani T., Uchida K.
  • Organizer: 6th Intercongress Symposium of the Asia Oceania Society for Comparative Endocrinology
  • Place of Presentation: New Zealand
• [Presentation] クロヌタウナギにおける組換え生殖腺刺激ホルモンの構築 (2009)
  • Author(s): 内田勝久、亀井宏泰、森山俊介、野崎眞澄, 他2名
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-19
• [Presentation] クロヌタウナギの腺下垂体細胞に対するエストロジェンとテストステロン投与の影響 (2009)
  • Author(s): 野崎眞澄、本田香織、三木誠、下谷豊和、内田勝久
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-19
• [Presentation] ヌタウナギの新規RFamideペプチドの同定と生理作用の解析 (2009)
  • Author(s): 大杉知裕、内田勝久、野崎眞澄、筒井和義
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-19
• [Presentation] ホヤ下垂体研究の現状-マボヤとカタユウレイボヤの比較ー (2009)
  • Author(s): 寺門潔、下谷豊和、野崎眞澄
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-19
• [Presentation] Identification of hagfish gonadotropin (GTH) and cellular distribution of GTH and adrenocorticotropin (ACTH) in the hagfish adenohypophysis (2009)
  • Author(s): Nozaki M., Uchida K., Miki M., Shimotani T., Honda K., Moriyama, S
  • Organizer: The 16th International Congress of Comparative Endocrinology
  • Place of Presentation: Hong Kong
• [Presentation] Novel hagfish hypothalamic RFamide peptides: isolation, localization and biological activity (2009)
  • Author(s): Osugi T., Uchida K., Nozaki M., Sower S.A., Tsutsui K
  • Organizer: The 16th International Congress of Comparative Endocrinology
  • Place of Presentation: Hong Kong
• [Presentation] Identification of hagfish gonadotropin (GTH) and cellular distribution of GTH and adrenocrticotropin (ACTH) in the hagfish adenohypophysis (2009)
  • Author(s): Nozaki M., Uchida K., Miki M., Shimotani T., Honda K., Moriyama, S.
  • Organizer: The 16th International Congress of Comparative Endocrinology
  • Place of Presentation: Hong Kong
• [Presentation] Novel hagfish hypothalamic RFamide peptides : isolatiom, localization and biological avtivity. (2009)
  • Author(s): Osugi T., Uchida K., Nozaki M., Sower S.A., Tsutsui K.
  • Organizer: The 16th International Congress of Comparative Endocrinology
  • Place of Presentation: Hong Kong
• [Presentation] クロヌタウナギにおける生殖腺刺激ホルモンの単離・精製と機能解析 (2008)
  • Author(s): 内田勝久、森山俊介、千葉洋明、下谷豊和、野崎眞澄
  • Organizer: 日本比較内分泌学会第33回大会
  • Place of Presentation: 広島
  • Year and Date: 2008-12-06
• [Presentation] クロヌタウナギにおける生殖腺刺激ホルモンの単離・精製と機能解析 (2008)
  • Author(s): 内田勝久, 他4名
  • Organizer: 日本比較内分泌学会第33回大会
  • Place of Presentation: 広島大学学士会館
  • Year and Date: 2008-12-06
• [Presentation] Identity of hagfish gonadotropin (GTH) and cellular distribution of GTH and adrenocorticotropin (ACTH) in the hagfish adenohypophysis (2008)
  • Author(s): Nozaki M., Uchida K., Miki M., Shimotani S., Honda K., Moriyama S
  • Organizer: The 15th CDB Meeting, "Advances in Cyclostome Research: Body plan and developmental programs before jawed vertebrates
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2008-01-25
• [Presentation] Identity of hagfish gonadotropin (GTH) and cellular distribution of GTH and adrenocorticotropin (ACTH) in the hagfish adenohypophysis. (2008)
  • Author(s): Nozaki M., Uchida K., Miki M., Shimotani S., Honda K. and Moriyama S.
  • Organizer: The 15th CDB Meeting, "Advances in Cyclostome Research: Body plan and developmental programs before jawed vertebrates
  • Place of Presentation: RIKEN Kobe CDB Institute
  • Year and Date: 2008-01-25
• [Presentation] Identification of hagfish gonadotropin (GTH) and its evolutionary implication (2007)
  • Author(s): Nozaki M., Shimotani S., Uchida K., Moriyama S
  • Organizer: Okazaki Biology Conference, OBC 6 on "Marine Biology"
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2007-12-03
• [Presentation] Identification of hagfish gonadotropin (GTH) and its evolutionary implication. (2007)
  • Author(s): Nozaki M., Shimotani S., Uchida K. and Moriyama S.
  • Organizer: Okazaki Biology Conference, OBC 6 on "Marine Biology"
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2007-12-03
• [Presentation] 最古の脊椎動物・ヌタウナギの生殖腺刺激ホルモン (2007)
  • Author(s): 内田勝久
  • Organizer: 平成19年度東京大学海洋研究所共同利用研究集会「魚類の適応と進化の統合生物学:遺伝子から行動まで」
  • Place of Presentation: 東京
  • Year and Date: 2007-11-16
• [Presentation] 最古の脊椎動物・ヌタウナギの生殖腺刺激ホルモン (2007)
  • Author(s): 内田勝久
  • Organizer: 平成19年度東京大学海洋研究所共同利用研究集会「魚類の適応と進化の統合生物学:遺伝子から行動まで」
  • Place of Presentation: 東京大学海洋研究所
  • Year and Date: 2007-11-16
• [Presentation] 日本比較内分泌学会第32回大会 (2007)
  • Author(s): 野崎眞澄、下谷豊和、内田勝久、森山俊介、高橋明義、川内浩司、Stacia A.Sower
  • Organizer: 日本比較内分泌学会第32回大会
  • Place of Presentation: 栃木
  • Year and Date: 2007-10-12
• [Presentation] 無顎類からみた下垂体と腺下垂体ホルモンの進化 (2007)
  • Author(s): 野崎眞澄、下谷豊和、内田勝久、森山俊介、高橋明義、川内浩司、Stacia A. Sower
  • Organizer: 日本比較内分泌学会第32回大会
  • Place of Presentation: 栃木県・日光プリンスホテル
  • Year and Date: 2007-10-12
• [Presentation] クロヌタウナギの下垂体における生殖腺刺激ホルモン遺伝子発現解析 (2007)
  • Author(s): 内田勝久、森山俊介、本田香織、下谷豊和、野崎眞澄
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 青森
  • Year and Date: 2007-09-20
• [Presentation] クロヌタウナギの腺下垂体におけるACTH細胞の同定 (2007)
  • Author(s): 野崎眞澄、下谷豊和、内田勝久、安田明和
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 青森
  • Year and Date: 2007-09-20
• [Presentation] クロヌタウナギの下垂体における生殖腺刺激ホルモン遺伝子発現解析 (2007)
  • Author(s): 内田勝久、森山俊介、本田香織、下谷豊和、野崎眞澄
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前大学
  • Year and Date: 2007-09-20
• [Presentation] クロヌタウナギの腺下垂体におけるACTH細胞の同定 (2007)
  • Author(s): 野崎眞澄、下谷豊和、内田勝久、安田明和
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前大学
  • Year and Date: 2007-09-20
• [Presentation] 新潟県産クロヌタウナギの生殖腺刺激ホルモン遺伝子の単離を発端に (2007)
  • Author(s): 内田勝久
  • Organizer: 日本下垂体研究会第22回学術集会
  • Place of Presentation: 神奈川
  • Year and Date: 2007-08-25
• [Presentation] 新潟県産クロヌタウナギの生殖腺刺激ホルモン遺伝子の単離を発端に (2007)
  • Author(s): 内田勝久
  • Organizer: 日本下垂体研究会第22回学術集会
  • Place of Presentation: 神奈川県・湘南国際村センター国際会議場
  • Year and Date: 2007-08-25