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Molecular cloning of cDNAs encoding bioactive peptides by the sequence capture method

Research Project

Project/Area Number 19570063
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionToho University

Principal Investigator

IWAMURO Shawichi  Toho University, 理学部, 准教授 (70221794)

Co-Investigator(Kenkyū-buntansha) KIKUYAMA Sakae  早稲田大学, 教育・総合科学学術院, 名誉教授 (20063638)
KOBAYASHI Tetsuya  埼玉大学, 理工学研究科, 准教授 (00195794)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords動物形態 / 両生類 / 抗菌ペプチド / 皮膚 / 遺伝子クローニング / ヒストンH2B / ファブリキウス嚢 / シュレーゲルアオガエル / 抗菌活性 / ハチ毒メリチン様ペプチド / ヒストンH4 / 細胞溶解性
Research Abstract

本研究では、両生類の皮膚から抗菌性のペプチドの単離を行なうとともに、その効率的な遺伝子クローニング法の開発を行なった。その結果、日本産の数種類のカエルから有用な抗菌ペプチド及びその配列を多数見つけ出すとともに、抗菌ペプチド遺伝子の発現には甲状腺ホルモンが重要であることを示した。また、ヒストンH2Bという本来は細胞の核にあるタンパク質がカエルの皮膚から得られていることに基づき、その抗菌メカニズムを明らかにした。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report

Research Products

(42 results)

All 2009 2008 2007

All Journal Article Presentation

  • [Journal Article] Developmental and thyroid hormone induced expression of preprotemporin genes in the skin of Japanese mountain brown frog Rana ornativentris2009

    • Author(s)
      Ohnuma A, Conlon JM, Iwamuro S
    • Journal Title

      Annals NY Acad Sci (in press)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Dermal cytolytic peptides and the system of innate immunity in anurans2009

    • Author(s)
      Conlon JM, Iwamuro S, King JD
    • Journal Title

      Annals NY Acad Sci (in press)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Demonstration of the proopiomelaocortin signalling system in the primary immune organ, the bursa of Fabricius, of the quail2009

    • Author(s)
      Kobayashi T, Takaso A, Tanaka S, Amano M, Takahashi A, Iwamuro S, Machida, T
    • Journal Title

      Annals NY Acad Sci (in press)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Developmental and thyroid hormone-induced expression of preprotemporin genes in the skin of Japanese mountain brown frog Rana ornativentris.2009

    • Author(s)
      Ohnuma A, Conlon JM, Iwamuro S
    • Journal Title

      Annals of New York Academy of Sciences (im press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dermal cytolytic peptides and the system of innate immunity in anurans.2009

    • Author(s)
      Conlon JM, Iwamuro S, King JD
    • Journal Title

      Annals of New York Academy of Sciences (im press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Demonstration of the proopiomelaocortin signalling system in the primary immune organ, the bursa of Fabricius, of the quail.2009

    • Author(s)
      Kobayashi T, Takaso A, Tanaka S, Amano M, Takahashi A, Iwamuro S, Machida T
    • Journal Title

      Annals of New York Academy of Sciences (im press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potential roles of histones in host defense as antimicrobial agents2008

    • Author(s)
      Kawasaki H, Iwamuro S
    • Journal Title

      Infectious Disorder-Drug Targets 8

      Pages: 195-205

    • Related Report
      2008 Final Research Report
  • [Journal Article] Antimicrobial action of histone H2B in Escherichia coli : evidence for membrane translocation and DNA-binding of a histone H2B fragment after proteolytic cleavage by outer membrane proteinase T2008

    • Author(s)
      Kawasaki H, Koyama T, Conlon JM, Yamakura F, Iwamuro S
    • Journal Title

      Biochimie 90

      Pages: 1693-1702

    • Related Report
      2008 Final Research Report
  • [Journal Article] Molecular cloning of a cDNA encoding atypical antimicrobial and cytotoxic brevinin-1Ja from the skin of the Japanese brown frog, Rana japonica2008

    • Author(s)
      Koyama T, Iwamuro S
    • Journal Title

      Zool Sci 25

      Pages: 487-491

    • Related Report
      2008 Final Research Report
  • [Journal Article] Characterization of a hemolytic protein, identified as histone H4, from the skin of the Japanese tree frog Hyla japonica(Hylidae)2008

    • Author(s)
      Kawasaki H, Iwamuro S, Goto Y, Nielsen Per F, Conlon JM
    • Journal Title

      Comp Biochem Phys B 149

      Pages: 120-125

    • Related Report
      2008 Final Research Report
  • [Journal Article] Antimicrobial action of histone H2B in Escherichia coli : evidence for membrane translocation and DNA-binding of a histone H2B fragment after proteolytic cleavage by outer membrane proteinase T.2008

    • Author(s)
      Kawasaki H, Koyama T, Conlon JM, Yamakura F, Iwamuro S.
    • Journal Title

      Biochimie 90

      Pages: 1693-1702

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potential roles of histones in host defence as antimicrobial agents.2008

    • Author(s)
      Kawasaki H, Iwamuro S.
    • Journal Title

      Drug Targets Infectious Disorders 8

      Pages: 195-205

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a hemolytic protein, identified as histone H4, from the skin of the Japanese tree frog Hyla japonica(Hylidae).2008

    • Author(s)
      Kawasaki H, Iwamuro S, Goto Y, Nielsen PF, Conlon JM.
    • Journal Title

      Comp Biochem Physiol B Biochem Mol Biol. 149

      Pages: 120-125

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning of a cDNA encoding the atypical antimicrobial and cytotoxic brevinin-1Ja from the skin of Japanese brown frog Rana japonica.2008

    • Author(s)
      Koyama T, Iwamuro S.
    • Journal Title

      Zoological Science (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evidence that the genes encoding the melittin-related peptides in the skins of the Japanese frogs Rana sakuraii and Rana tagoi are not orthologous to bee venom melittin genes : developmental-and tissue-dependent gene expression2007

    • Author(s)
      Suzuki H, Conlon JM, Iwamuro, S
    • Journal Title

      Peptides 28

      Pages: 2061-2068

    • Related Report
      2008 Final Research Report
  • [Journal Article] Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog, Rana dybowskii2007

    • Author(s)
      Conlon JM, Kokodziejek J, Nowotny N, Leprince J, Vaudry H, Coquet L, Jouenne T, Iwamuro S
    • Journal Title

      Toxicon 50

      Pages: 746-756

    • Related Report
      2008 Final Research Report
  • [Journal Article] Expression of the estrogen receptor a gene in the anal fin of Japanese medaka, Oryzias latipes, by environmental concentrations of bisphenol A2007

    • Author(s)
      Hayashi H, Nishimoto A, Oshima N, Iwamuro S
    • Journal Title

      J Toxicol Sci 32

      Pages: 91-96

    • Related Report
      2008 Final Research Report
  • [Journal Article] Antimicrobial peptides from the skin of the frog, Rana dybowskii implications for the phylogeny of the Japanese brown frogs.2007

    • Author(s)
      Conlon JM, Kolodziejek J, Nowotny N, Leprince J, Vaudry H, Coquet L, Jouenne T, Iwamuro, S.
    • Journal Title

      Toxicon 50

      Pages: 746-756

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evidence that the genes encoding the melittin-related peptides in the skins of the Japanese frogs Rana sakuraii and Rana tagoi are not orthologous to bee venom melittin genes: developmental-and tissue-dependent gene expression.2007

    • Author(s)
      Suzuki H, Conlon JM, Iwamuro S.
    • Journal Title

      Peptides 28

      Pages: 2061-2068

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 対馬産Rana属皮膚抗菌ペプチドの遺伝子クローニング2009

    • Author(s)
      秋葉貴久、安藤麻理、Conlon JM、岩室祥一
    • Organizer
      日本動物学会関東支部第61回大会
    • Place of Presentation
      埼玉大学
    • Year and Date
      2009-03-20
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ウシガエルハーダー腺における抗菌ペプチド遺伝子の発現2009

    • Author(s)
      小西健司、蓮沼至、小林哲也、岡田令子、中野真樹、白間一彦、菊山榮、岩室祥一
    • Organizer
      日本動物学会関東支部第61回大会
    • Place of Presentation
      埼玉大学
    • Year and Date
      2009-03-20
    • Related Report
      2008 Final Research Report
  • [Presentation] ゲノム情報から両生類抗菌ペプチド遺伝子の発現調節機構を探る2009

    • Author(s)
      宮崎希、太枝志帆、竹山留美子、大沼彩、岩室祥一
    • Organizer
      日本動物学会関東支部第61回大会
    • Place of Presentation
      埼玉大学
    • Year and Date
      2009-03-20
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 無尾目両生類皮膚腺の形態学的解析2009

    • Author(s)
      丸橋佳織、岩室祥一
    • Organizer
      日本動物学会関東支部第61回大会
    • Place of Presentation
      埼玉大学
    • Year and Date
      2009-03-20
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 鳥類血球系及び免疫系からのCathelicidin遺伝子のクローニング2009

    • Author(s)
      椿卓、小林哲也、稲嶺由羽、菊山榮、岩室祥一
    • Organizer
      日本動物学会関東支部第61回大会
    • Place of Presentation
      埼玉大学
    • Year and Date
      2009-03-20
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ウシガエルバーダー腺における抗菌ペプチド遺伝子の発現2009

    • Author(s)
      小西健司, 蓮沼至, 小林哲也, 岡田令子, 中野真樹, 自間一彦, 菊山榮, 岩室祥一
    • Organizer
      日本動物学会関東支部第61回大会
    • Place of Presentation
      埼玉大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒストンH2Bの抗大腸菌作用機序の解析2008

    • Author(s)
      岩室祥一、川崎広明、山倉文幸、Conlon J M.
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2008-12-10
    • Related Report
      2008 Final Research Report
  • [Presentation] 両生類皮膚抗菌ペプチドの遺伝子発現とその甲状腺ホルモンによる関与2008

    • Author(s)
      岩室祥一、太枝志帆、鈴木啓恵、大沼彩
    • Organizer
      第33回日本比較内分泌学会大会
    • Place of Presentation
      広島大学
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ニホンアカガエル新規抗菌ペプチドBrevenin-1Ja2008

    • Author(s)
      岩室祥一、小山卓己
    • Organizer
      第60回日本動物学会関東支部大会
    • Place of Presentation
      東京大学
    • Year and Date
      2008-03-22
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] ニホンアカガエル抗菌ペプチドの遺伝子クローニングとその解析2008

    • Author(s)
      小山卓己、岩室祥一
    • Organizer
      第60回日本動物学会関東支部大会
    • Place of Presentation
      東京大学
    • Year and Date
      2008-03-22
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] Molecular cloning and developmental expression of genes encoding antimicrobial peptide precursors in Rana ornativentris2008

    • Author(s)
      Iwamuro S, Oeda S, Miyazaki N, Conlon JM, Ohnuma A
    • Organizer
      The 24th Conference of European Comparative Endocrinologists 2008, Genoa, Italy
    • Place of Presentation
      Centro Congressi Magazzini del Cotone Centro Congressi Magazzini del Cotone
    • Related Report
      2008 Final Research Report
  • [Presentation] Identification of Des-acetyl-alpha-melanocyte-stimulating hormone in the quail bursa of Fabricius2008

    • Author(s)
      Kobayashi T, Takaso A, Tanaka S, Amano M, Takahashi A, Iwamuro S, Machida T
    • Organizer
      The 24th Conference of European Comparative Endocrinologists 2008, Genoa, Italy
    • Place of Presentation
      Centro Congressi Magazzini del Cotone
    • Related Report
      2008 Final Research Report
  • [Presentation] Molecular cloning and developmental expression of genes encoding antimicrobial peptide precursors in Ranaornativentris.2008

    • Author(s)
      Iwamuro s, Oeda S, Miyazaki N, Conlon J, Ohnuma A.
    • Organizer
      The 24th Conference of European Comparative Endocrinologists 2008
    • Place of Presentation
      Genoa, Italy
    • Related Report
      2008 Annual Research Report
  • [Presentation] Dermal cytolytic peptides and the system of innate immunity in amphibians.2008

    • Author(s)
      Conlon JM, Iwamuro S.
    • Organizer
      The 24th Conference of European Comparative Endocrinologists 2008
    • Place of Presentation
      Genoa, Italy
    • Related Report
      2008 Annual Research Report
  • [Presentation] Identification of Des-acetyl-alpha-melanocyte-stimulating hormone in the quail bursa of Fabricius.2008

    • Author(s)
      Kobayashi T, Takaso A, Tanaka S, Amano M, Takahashi A, Iwamuro S, Machida T.
    • Organizer
      The 24th Conference of European Comparative Endocrinologists 2008
    • Place of Presentation
      Genoa, Italy
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒストンH2Bの抗大腸菌作用機序の解析2008

    • Author(s)
      岩室祥一, 川崎広明, 山倉文幸, Conlon JM
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2008 Annual Research Report
  • [Presentation] 微生物を撃て! -カエルの抗菌ペプチド2007

    • Author(s)
      岩室祥一
    • Organizer
      日本動物学会第78回大会シンポジウム「両生類はワンダーランド」
    • Place of Presentation
      弘前大学
    • Year and Date
      2007-09-22
    • Related Report
      2008 Final Research Report
  • [Presentation] ヤマアカガエル抗菌ペプチドの遺伝子クローニングとそのステージ依存的な発現2007

    • Author(s)
      大沼彩、鈴木啓恵、Conlon, JM、岩室祥一
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前大学
    • Year and Date
      2007-09-20
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] 組換え体ヒストンH2Bの抗大腸菌作用機構2007

    • Author(s)
      川崎広明、Conlon JM、小林哲也、岩室祥一
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前大学
    • Year and Date
      2007-09-20
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] ニホンアカガエル由来抗菌ペプチドJaponicinの遺伝子クローニング及び局在解析2007

    • Author(s)
      小山卓己、Conlon JM、岩室祥一
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前大学
    • Year and Date
      2007-09-20
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] アカガエル属抗菌ペプチドTemporinの遺伝子解析2007

    • Author(s)
      太枝志帆、大沼彩、竹山瑠美子、Conlon JM、岩室祥一
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前大学
    • Year and Date
      2007-09-20
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] 両生類下垂体研究にまつわるささやかなセレンディピティー2007

    • Author(s)
      菊山 榮
    • Organizer
      日本動物学会第78回大会シンポジウム「両生類はワンダーランド」
    • Place of Presentation
      弘前大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] 微生物を撃て!-カエルの抗菌ペプチド2007

    • Author(s)
      岩室祥一
    • Organizer
      日本動物学会第78回大会シンポジウム「両生類はワンダーランド」
    • Place of Presentation
      弘前大学
    • Related Report
      2007 Annual Research Report

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Published: 2007-03-31   Modified: 2016-04-21  

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