Transport Mechanism of Vesicular Glutamate Transporter

• Project/Area Number: 19570132
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Okayama University
• Principal Investigator: OMOTE Hiroshi Okayama University, 大学院・医歯薬学総合研究科, 准教授 (10273707)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 生体膜・チャネル・トランスポーター・能動輸送 / タンパク質 / 生化学 / 脳・神経 / 小胞型グルタミン酸トランスポーター / 大量発現 / 精製 / 変異導入 / リン酸輸送

Research Abstract

小胞型グルタミン酸トランスポーター(VGLUT)はV-ATPaseがATPの加水分解によって形成するH+の電気化学的勾配を駆動力とし、グルタミン酸を小胞内に輸送するトランスポーターである。この研究ではVGLUTによるグルタミン酸輸送の分子機構を明らかにすべく、VGLUTを含むSLC17型ファミリートランスポーターの輸送機構を速度論的に解析した。

Research Products

• [Journal Article] 小胞型神経伝達物質トランスポーターの局在化機構 (2008)
  • Author(s): 表弘志、森山芳則
  • Journal Title: 蛋白質核酸酵素 53 Pages: 2220-2224
  • Peer Reviewed
• [Journal Article] Identification of a vesicular aspartate transporter (2008)
  • Author(s): Miyaji T., Echigo N., Hiasa M., Senoh S., Omote H., Moriyama Y.
  • Journal Title: Proc Natl Acad Sci U S A 105 Pages: 11720-11724
  • Peer Reviewed
• [Journal Article] Multidrug and toxic compound extrusion(MATE)-type proteins as anchor transporters for the excretion of metabolic waste products and xenobiotics (2008)
  • Author(s): *Moriyama Y., Hiasa M., Matsumoto T., Omote H.
  • Journal Title: Xenobiotica 38 Pages: 1107-1118
  • Peer Reviewed
• [Journal Article] Identification of a vesicular nucleotide transporter (2008)
  • Author(s): Sawada K., Echigo N., Juge N., Miyaji T., Otsuka M., Omote H., Yamamoto A., *Moriyama Y.
  • Journal Title: Proc Natl Acad Sci U S A 105 Pages: 5683-5686
  • NAID: 120002306983
  • Peer Reviewed
• [Journal Article] Role of glutamate residues in substrate recognition by human MATE1 polyspecific H^+/organic cation exporter (2008)
  • Author(s): Matsumoto T., Kanamoto T., Otsuka M., Omote H., *Moriyama Y.
  • Journal Title: Am J Physiol Cell Physiol 294
  • Peer Reviewed
• [Journal Article] 薬物排出のアンカートランスポーター、MATEの構造と機能 (2008)
  • Author(s): 日浅未来、表弘志、森山芳則
  • Journal Title: 蛋白質核酸酵素 53 Pages: 52-58
  • NAID: 40015771165
  • Peer Reviewed
• [Journal Article] A novel variant of mouse MATE-1 H^+/organic cation antiporter with a long hydrophobic tail (2008)
  • Author(s): Kobara A., Hiasa M., Matsumoto T., Otsuka M., Omote H., *Moriyama Y.
  • Journal Title: Arch Biochem Biophys 469 Pages: 195-199
  • Peer Reviewed
• [Journal Article] Identification of a vesicular aspartate transporter. (2008)
  • Author(s): Miyaji T., et al
  • Journal Title: Proc Natl Acad Sci U S A. 105 Pages: 11720-11724
  • Peer Reviewed
• [Journal Article] Multidrug and toxic compound extrusion (MATE)-type proteins as anchor transporters for the excretion of metabolic waste products and xenobiotics. (2008)
  • Author(s): Moriyama Y., et al
  • Journal Title: Xenobiotica 38 Pages: 1107-1118
  • Peer Reviewed
• [Journal Article] Role of glutamate residues in substrate recognition by human MATE1 polyspecific H+/organic cation exporter (2008)
  • Author(s): Matsuraoto T., et al
  • Journal Title: Am J Physiol Cell Physiol. 294
  • Peer Reviewed
• [Journal Article] Identification of a vesicular nucleotide transporter. (2008)
  • Author(s): Sawada K, Echigo N, Juge N, Miyaj
  • Journal Title: Proc. Natl. Acad. USA 105(電子版)
  • Peer Reviewed
• [Journal Article] Functional characterization of testis-specific rodent multidrug and toxic compound extrusion 2, a class III MATE-type polyspecific H+/organic cation exporter (2007)
  • Author(s): Hiasa M., Matsumoto T., Komatsu T., Omote H., *Moriyama Y
  • Journal Title: Am J Physiol Cell Physiol 293
  • Peer Reviewed
• [Journal Article] 小胞型グルタミン酸トランスポーターの分子機構 (2007)
  • Author(s): 表弘志、樹下成信
  • Journal Title: 生化学 79 Pages: 956-960
  • NAID: 10019771904
  • Peer Reviewed
• [Presentation] Functional Characterization of Purified Human Multidrug And Toxic Compound Extrusion 1 (2009)
  • Author(s): Hiroshi Omote
  • Organizer: JBMB
  • Place of Presentation: Kobe
  • Year and Date: 2009-12-09
• [Presentation] Xenobiotic Transport By The MATE Family In Prokaryotes (2009)
  • Author(s): Hiroshi Omote
  • Organizer: Gordon Research Conference
  • Place of Presentation: Galveston, TX, USA
  • Year and Date: 2009-03-23
• [Presentation] SLC17型トランスポーターの新メンバー、小胞型ヌクレオチドトランスポーター(VNUT)の発見 (2008)
  • Author(s): 表弘志
  • Organizer: 生体エネルギー研究会
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2008-11-07
• [Presentation] 小胞型抑制性アミノ酸トランスポーター(VIAAT)の輸送機構解析 (2007)
  • Author(s): 表弘志
  • Organizer: 生体エネルギー研究会
  • Place of Presentation: 山口大学
  • Year and Date: 2007-11-16