Establishment of hierarchical structure analysis of proteins insolutions by high-throughput of wide-angle X-rays scattering usingsynchrotron radiation source
Project/Area Number |
19570148
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biophysics
|
Research Institution | Gunma University |
Principal Investigator |
HIRAI Mitsuhiro Gunma University, 大学院・工学研究科, 教授 (00189820)
|
Project Period (FY) |
2007 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
|
Keywords | タンパク質 / 生体膜 / 階層構造 / フォールディング / アミロイド形成 / X線広角散乱 / 放射光 / フオールディング / フォールデング / アミロイド / 放射光X線 / 溶液散乱 |
Research Abstract |
The wide-angle X-ray scattering using the third generation synchrotron radiation source is a promising and powerful method elucidating in-situ hierarchical structures of proteins at those functional states. By the excution of this study aimed for the establishment of the solution X-ray wide-angle scattering, we can get scattering data with high statistics precision while avoiding radiation damage, and we clarified the relation between hydration shell and structural transition of a protein, the all processes of nucleation / growth in the amyloid metastasis, the change of dynamics of a protein induced by the amyloid transition (α-to-cross-β).We published the aobve results of this project in 11 full articles in academic journals and in 23 papers at overseas international conferences and at domestic academic society meetings.
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Report
(4 results)
Research Products
(54 results)