• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the function of cofilin in actin cytoskeleton dynamics

Research Project

Project/Area Number 19570174
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionTohoku University

Principal Investigator

OHASHI Kazumasa  Tohoku University, 大学院・生命科学研究科, 准教授 (10312539)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords細胞骨格・運動 / アクチン / コフィリン / LIMキナーゼ / 蛍光蛋白質 / 阻害剤 / アクチン骨格 / リン酸化 / GFP / Dronpa / イメージング / Slingshot
Research Abstract

アクチン線維の切断・脱重合因子コフィリンは、細胞運動の動的な変化に必須な蛋白質であり、その働きはリン酸化酵素HMキナーゼによって負に制御される。本研究において、GFPを用いたL]]M[キナーゼ阻害剤の探索方法を開発し、HMキナーゼ特異的な阻害剤を発見した。この化合物は癌細胞の移動を阻害することが明らかとなった。また、ラメリポディア形成においてコフィリンは遊離アクチンの産生において寄与し、IJMキナーゼはアクチン線維の伸長において寄与していることを明らかにした。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (47 results)

All 2009 2008 2007 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (29 results) Remarks (4 results)

  • [Journal Article] MST2-and Furry-Mediated Activation of NDR1 Kinase Is Critical for Precise Alignment of Mitotic Chromosomes.2009

    • Author(s)
      Chiba S., Ikeda M., Katsunuma K., Ohashi K. and Mizuno K.
    • Journal Title

      Curr. Biol. 19

      Pages: 675-681

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular dissection of the mechanisms of substrate recognition and F-actin-mediated activation of cofilin-phosphatase Slingshot-12009

    • Author(s)
      Chiba S., et.al.
    • Journal Title

      Current Biology 19

      Pages: 1-7

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular dissection of the mechanisms of substrate recognition and F-actin-mediated activation of cofilin-phosphatase slingshot-12008

    • Author(s)
      Kurita S., Watanabe Y., Gunji E., Ohashi K. and Mizuno K.
    • Journal Title

      J. Biol. Chem. 283

      Pages: 32545-32552

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Critical roles of actin-interacting protein 1 in cytokinesis and chemotacticmigration of mammalian cells2008

    • Author(s)
      Kato A., Kurita S., Hayashi A., Kaji N., Ohashi K. and Mizuno K.
    • Journal Title

      Biochem. J. 414

      Pages: 261-270

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of the invasive capacity of rat ascites hepatoma cells by knockdown of Slingshot or LIM kinase2008

    • Author(s)
      Horita Y., Ohashi K., Mukai M., Inoue M. and Mizuno K.
    • Journal Title

      J. Biol. Chem. 283

      Pages: 6013-6021

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular dissection of the mechanisms of substrate recognition-and F-actin-mediated activation of cofilin-phosphatase Slingshot-12008

    • Author(s)
      Kurita S., et.al.
    • Journal Title

      J. Biol. Chem 283

      Pages: 32542-32552

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Critical roles of actin-interacting protein 1 in cytokinesis and chemotactic migration of mammalian cells2008

    • Author(s)
      Kato A., et.al.
    • Journal Title

      Biochemical J 414

      Pages: 261-270

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of the Invasive Capacity of Rat Ascites Hepatoma Cells by Knockdown of Slingshot or LIM Kinase2008

    • Author(s)
      Horita, et. al.
    • Journal Title

      J. Biol. Chem. 283

      Pages: 6013-6021

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Actin filaments-stabilizing and -bundling activities of cofilin-phosphatase Slingshot-12007

    • Author(s)
      Kurita S., Gunji E., Ohashi K. and Mizuno K.
    • Journal Title

      Genes Cells 12

      Pages: 663-676

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cofilin promotes stimulus-inducedlamellipodium formationby generating an abundant supplyof actin monomers2007

    • Author(s)
      Kiuchi T., Ohashi K., Kurita S. and Mizuno K.
    • Journal Title

      J. Cell Biol. 177(3)

      Pages: 465-476

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] LIM kinase and slingshot are critical for neurite extension2007

    • Author(s)
      Endo M., Ohashi K., and Mizuno K.
    • Journal Title

      J Biol Chem. 282

      Pages: 13692-13702

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] LIM-kinase and slingshot are critical for neurite extension2007

    • Author(s)
      Endo, M., et. al.
    • Journal Title

      J. Biol. Chem. 282

      Pages: 13692-136702

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cofilin critically contributes to stimulus-induced lamellipodium formation by generating an abundant supply of actin monomers in the cytoplasm2007

    • Author(s)
      Kiuchi, T., et. al.
    • Journal Title

      J. Cell Biol. 177

      Pages: 465-476

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Actin filaments-stabilizing and-bundling activities of cofilin-phosphatase Slingshot-12007

    • Author(s)
      Kurita, S., et. al.
    • Journal Title

      Genes Cells 12

      Pages: 663-673

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Wnt-PCP経路による神経突起退縮に関与するRhoA-GEFの同定2008

    • Author(s)
      辻拓史, 太田裕作, 太橋一正, 水野健作
    • Organizer
      日本生化学会東北支部例会
    • Place of Presentation
      盛岡
    • Year and Date
      2008-05-17
    • Related Report
      2008 Final Research Report
  • [Presentation] Analysis of the regulation of actin cytoskeleton dynamics using Dronpa, a photochromic fluorescent protein2008

    • Author(s)
      辻拓史, 他
    • Organizer
      日本生化学会東北支部例会
    • Place of Presentation
      盛岡
    • Year and Date
      2008-05-17
    • Related Report
      2008 Annual Research Report
  • [Presentation] Phosphorylation of Slingshot-1 by Polo-like kinase-12008

    • Author(s)
      Otsuki M., Ikebe C., Ohashi K. and Mizuno K.
    • Organizer
      The 48th Annual Meeting of The American Society for Cell Biology
    • Place of Presentation
      San Francisco
    • Related Report
      2008 Final Research Report
  • [Presentation] MST2, MST3によるNDR1の活性制御と細胞分裂の制御cell movement mediated by cell adhesion2008

    • Author(s)
      池田真教, 葉秀平, 勝沼功吉, 太橋二正, 水野健作
    • Organizer
      BMB2008第81回日本生化学会大会・第31回日本分子生物学会年会・合同大会
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] 哺乳類細胞の対称分裂における紡錘体位置決定タンパク質の局在とアクチン骨格の関与movement from epiblast to presomitic mesoderm2008

    • Author(s)
      林文, 黒沢豪, 梶紀子, 大橋一正, 鎌倉幸子, 住本英樹, 水野健作
    • Organizer
      BMB2008第81回日本生化学会大会・第31回日本分子生物学会年会・合同大会
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] ラメリポディア形成過程における細胞内Gーアクチン濃度のタイムラプスイメージングmolecule associates with Notch signaling in early neural differentiation2008

    • Author(s)
      永井友朗, 木内泰, 大橋一正, 水野健作
    • Organizer
      BMB2008第81回日本生化学会大会・第31回日本分子生物学会年会・合同大会
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] Analysis of the regulation of actin cytoskeleton dynamics using Dronpa, a photochromic fluorescent protein2008

    • Author(s)
      Ohashi K., Kiuchi T., Nagai T. and Mizuno K.
    • Organizer
      MHS2008 (2008 International Symposium on Micro-NanoMechatronicsand Human Science)
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] Cofilin phospho-regulation plays a critical role in tumor cell motilityand invasion2008

    • Author(s)
      Ohashi K., Horita Y., and Mizuno K.
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] Roles of cofilin phosphorylation in neurite outgrowth and dendritogenesis outgrowth and dendritogenesis2008

    • Author(s)
      Ohashi K., Akatsuka J., Miyajima K., Endo M. and Mizuno K.
    • Organizer
      第31回日本神経科学大会ワークショップ
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] LIM-kinase is required for BDNF-induced dendritogenesis2008

    • Author(s)
      Miyajima K., Akatsuka J., Ohashi K. and Mizuno K
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Roles of cofilin phospho-regulation in tumor cell migration and invasion2008

    • Author(s)
      Ohashi K., Horita Y., Kiuchi T. and Mizuno K.
    • Organizer
      第60回日本細胞生物学会大会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] Phosphorylation of Slingshot-1 by Polo-like kinase-12008

    • Author(s)
      Otsuki M., Kaneto K., Ikebe C., Ohashi K. and Mizuno K.
    • Organizer
      第60回日本細胞生物学会大会
    • Place of Presentation
      横浜
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Involvement of cofilin/ADF, Slingshot and LIM-kinase in invadopodium formation of tumor cells2008

    • Author(s)
      Kitatani K., Horita Y. , Ohashi K. and Mizuno K.
    • Organizer
      第60回日本細胞生物学会大会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] Identification of Rho-GEFs that mediate Dishevelled-induced RhoA activation2008

    • Author(s)
      Tsuji T., Ohta Y., Ohashi K. and Mizuno K.
    • Organizer
      第60回日本細胞生物学会大会
    • Place of Presentation
      横浜
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Roles of cofilin phospho-regulation in tumor cell migration and invasion2008

    • Author(s)
      大橋一正, 他
    • Organizer
      第60回日本細胞生物学会大会
    • Place of Presentation
      横浜
    • Related Report
      2008 Annual Research Report
  • [Presentation] Involvement of cofilin/ADF, Slingshot and LIM-kinase in invado podium formation of tumor cells2008

    • Author(s)
      北谷佳奈恵, 他
    • Organizer
      第60回日本細胞生物学会大会
    • Place of Presentation
      横浜
    • Related Report
      2008 Annual Research Report
  • [Presentation] Roles of cofilin phosphorylation in neurite outgrowth and dendritogenesis2008

    • Author(s)
      大橋一正, 他
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] Cofilin phospho-regulation plays a critical role in tumor cell motility and invasion2008

    • Author(s)
      大橋一正, 他
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] Analysis of the regulation of actin cytoskeleton dynamics using Dronpa, a photochromic fluorescent protein2008

    • Author(s)
      大橋一正, 他
    • Organizer
      2008 International Symposium on Micro-NanoMechatronics and Human Science
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] MST2, MST3によるNDR1の活性制御と細胞分裂の制御2008

    • Author(s)
      池田真教, 他
    • Organizer
      BMB2008第81回日本生化学会大会・第31回日本分子生物学会年会・合同大会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] 哺乳類細胞の対称分裂における紡錘体位置決定タンパク質の局在とアクチン骨格の関与2008

    • Author(s)
      林文, 他
    • Organizer
      BMB2008第81回日本生化学会大会・第31回日本分子生物学会年会・合同大会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] ラメリポディア形成過程における細胞内G-アクチン濃度のタイムラプスイメージング-Dronpa-アクチンのFDAP解析を用いて-2008

    • Author(s)
      永井友朗, 他
    • Organizer
      BMB2008第81回日本生化学会大会・第31回日本分子生物学会年会・合同大会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] Phosphorylation of Slingshot-1 by Polo-like kinase-12008

    • Author(s)
      大槻美樹, 他
    • Organizer
      The 48th Annual Meeting of The American Society for Cell Biology
    • Place of Presentation
      サンフランシスコ(米国)
    • Related Report
      2008 Annual Research Report
  • [Presentation] コフィリンのリン酸化によるラメリポディア内のアクチンターンオーバー制御機構2007

    • Author(s)
      大橋一正, 他
    • Organizer
      第80回日本生化学会大会・第30回日本分子生物学会大会 合同大会
    • Place of Presentation
      横浜(パシフィコ横浜)
    • Year and Date
      2007-12-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] FurryによるNDR1の活性化とその中心体複製における役割2007

    • Author(s)
      千葉秀平, 他
    • Organizer
      第80回日本生化学会大会・第30回日本分子生物学会大会 合同大会
    • Place of Presentation
      横浜(パシフィコ横浜)
    • Year and Date
      2007-12-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] コフィリン脱リン酸化におけるSlingshot-1のN末端領域の機能2007

    • Author(s)
      栗田宗一, 他
    • Organizer
      第80回日本生化学会大会・第30回日本分子生物学会大会 合同大会
    • Place of Presentation
      横浜(パシフィコ横浜)
    • Year and Date
      2007-12-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] Critical roles of cofilin/ADF,Slingshot and LIM-kinase in tumor cell invasion2007

    • Author(s)
      堀田祐司, 他
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      横浜(パシフィコ横浜)
    • Year and Date
      2007-10-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] スリングショットによる海馬神経細胞樹状突起形成の制御2007

    • Author(s)
      赤塚淳一, 他
    • Organizer
      第30回日本神経科学大会・第50回日本神経化学会大会・第17回日本神経回路学会大会
    • Place of Presentation
      横浜(パシフィコ横浜)
    • Year and Date
      2007-09-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] コフィリンのリン酸化はラメリポディア内のアクチンターンオーバー速度とラメリポディアの長さを制御する2007

    • Author(s)
      大橋一正, 他
    • Organizer
      第40回日本発生生物学会・第59回日本細胞生物学会合同大会
    • Place of Presentation
      福岡(福岡国際会議場)
    • Year and Date
      2007-05-29
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.lifesci.tohoku.ac.jp/teacher/biomo1ecular/ts_oohashi.html

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.biology.tohoku.ac.jp/lab-www/mizunolab/

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.lifesci.tohoku.ac.jp/teacher/biomolecular/ts_oohashi.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.lifesci.tohoku.ac.jp/teacher/biomolecular/ts_oohashi.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi