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Function of the Itm2 family of membrane proteins on endosomes

Research Project

Project/Area Number 19570178
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionTokyo Institute of Technology

Principal Investigator

KOMADA Masayuki  Tokyo Institute of Technology, 大学院・生命理工学研究科, 准教授 (10225568)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords細胞 / タンパク質 / ユビキチン / 脱ユビキチン化酵素 / メンブレントラフィック / 選別輸送 / エンドソーム / 癌 / 酵素 / 細胞・組織 / シグナル伝達 / 蛋白質 / 神経科学 / 痴呆 / 脳神経疾患
Research Abstract

遺伝性痴呆症の原因遺伝子産物であるItm2タンパク質が細胞内小器官であるエンドソームに局在するタンパク質複合体Hrs-STAMと結合することから、エンドソーム機能の痴呆症発症への関与が示唆されている。本研究では、エンドソームでHrs-STAM複合体と結合するItm2 タンパク質および脱ユビキチン化酵素UBPY とAMSH の解析を行い、1) Itm2タンパク質がエンドソームで働くこと、2) UBPYとAMSHが細胞質分裂においても重要な役割を果たすこと、そして3) AMSHの酵素活性の基質特異性の構造的基盤、を解明した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (48 results)

All 2009 2008 2007 Other

All Journal Article (26 results) (of which Peer Reviewed: 24 results) Presentation (18 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Nucleolar structure and function are regulated by the deubiquitylating enzyme USP362009

    • Author(s)
      Endo A, Matsumoto M, Inada T, Yamamoto A,Nakayama KI, Kitamura N, Komada M
    • Journal Title

      J. Cell Sci 122

      Pages: 678-686

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dispensable role of protein 4.1B/DAL-1 in rodent adrenal medulla regarding generation of pheochromocytoma and plasmalemmal localization of TSLC12009

    • Author(s)
      Ohno N, Terada N, Komada M, Saitoh S, Costantini F, Pace V, Germann PG, Weber K, Yamakawa H, Ohara O, Ohno S
    • Journal Title

      Biochim. Biophys. Acta 1793

      Pages: 506-515

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] ユビキチン修飾系による細胞質分裂の制御2009

    • Author(s)
      駒田雅之、向井明子
    • Journal Title

      蛋白質核酸酵素 54

      Pages: 11-19

    • NAID

      40016392332

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nucleolar structure and function are regulated by the deubiquitylating enzyme USP36.2009

    • Author(s)
      Endo A
    • Journal Title

      J. Cell Sci. 122

      Pages: 678-686

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dispensable role of protein 4.1B/DAL-1 in rodent adrenal medulla regarding generation of pheochromocytoma and plasmalemmal localization of TSLC1.2009

    • Author(s)
      Ohno N
    • Journal Title

      Biochim. Biophys. Acta 1793

      Pages: 506-515

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ユビキチン修飾系による細胞質分裂の制御2009

    • Author(s)
      駒田雅之
    • Journal Title

      蛋白質核酸酵素 54

      Pages: 11-19

    • NAID

      40016392332

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic regulation of ubiquitylation and deubiquitylation at the central spindle during cytokinesis2008

    • Author(s)
      Mukai A, Mizuno E, Kobayashi K, Matsumoto M, Nakayama KI, Kitamura N, Komada M
    • Journal Title

      J. Cell Sci 121

      Pages: 1325-1333

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for selective cleavage of Lys63-linked polyubiquitin chains2008

    • Author(s)
      Sato Y, Yoshikawa A, Yamagata A, Mimura H, Yamashita M, Ookata K, Nureki O, Iwai K, Komada M, Fukai S
    • Journal Title

      Nature 455

      Pages: 358-362

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sodium channel cluster, betaIV-spectrin and ankyrinG positive "hot spots" on dendritic segments of parvalbumin-containing neurons and some other neurons in the mouse and rat main olfactory bulbs2008

    • Author(s)
      Kosaka T, Komada M, Kosaka K
    • Journal Title

      Neurosci. Res 62

      Pages: 176-186

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ubiquitin-mediated proteasomal degradation of non-synonymous SNP variants of human ABC transporter ABCG22008

    • Author(s)
      Nakagawa H, Tamura A, Wakabayashi K, Hoshijima K, Komada M, Yoshida T, Kometani S, Matsubara T, Mikuriya K, Ishikawa T
    • Journal Title

      Biochem 411

      Pages: 623-631

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Controlling receptor downregulation by ubiquitination and deubiquitination2008

    • Author(s)
      Komada M
    • Journal Title

      Curr. Drug Discov. Technol 5

      Pages: 78-84

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] エンドソームにおけるメンブレントラフィックと細胞質分裂の接点2008

    • Author(s)
      向井明子、駒田雅之
    • Journal Title

      増刊号「メンブレントラフィックの奔流」 53

      Pages: 2094-2098

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for selective cleavage of Lys63-linked polyubiquitin chains.2008

    • Author(s)
      Sato Y
    • Journal Title

      Nature 455

      Pages: 358-362

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic regulation of ubiquitylation and deubiquitylation at the central spindle during cytokinesis.2008

    • Author(s)
      Mukai A
    • Journal Title

      J. Cell Sci. 121

      Pages: 1325-1333

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ubiquitin-mediated proteasomal degradation of non-synonymous SNP variants of human ABC transporter ABCG2.2008

    • Author(s)
      Nakagawa H
    • Journal Title

      Biochem. J. 411

      Pages: 623-631

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sodium channel cluster, betaIV-spectrin and ankyrinG positive hot spots on dendritic segments of parvalbumin-containing neurons and someother neurons in the mouse and rat main olfactory bulbs.2008

    • Author(s)
      Kosaka T
    • Journal Title

      Neurosci. Res. 62

      Pages: 176-186

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Controlling receptor downregulation by ubiquitination and deubiquitination.2008

    • Author(s)
      Komada M
    • Journal Title

      Curr. Drug Discov. Technol. 5

      Pages: 78-84

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] エンドソームにおけるメンブレントラフィックと細胞質分裂の接点2008

    • Author(s)
      向井明子
    • Journal Title

      蛋白質核酸酵素 53

      Pages: 2094-2098

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Controlling receptor downregulation by ubiquitination and deubiquitination.2008

    • Author(s)
      Komada, M.
    • Journal Title

      Curr. Drug. Discov. Technol. 5

      Pages: 78-84

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ubiquitin-mediated proteasomal degradation of non-synonymous SNP variants of human ABC transporter ABCG2.2008

    • Author(s)
      Nakagawa, H.
    • Journal Title

      Biochem. J. 411

      Pages: 623-631

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic regulation of ubiquitination and deubiquitination at the central spindle during cytokinesis.2008

    • Author(s)
      Mukai, A.
    • Journal Title

      J. Cell Sci. 121

      Pages: 1325-1333

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intramolecular disulfide hond is a critical checkpoint determining degradative fates of ATP-binding cassette (ABC) transporter ABCG2 protein.2007

    • Author(s)
      Wakabayashi, K.
    • Journal Title

      J. Biol. Chem. 282

      Pages: 27841-27846

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 14-3-3-dependent inhibition of the deubiquitinating activity of UBPY and its cancellation in the M phase.2007

    • Author(s)
      Mizuno, E
    • Journal Title

      Exp. Cell Res. 313

      Pages: 3624-3634

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interaction of membrane skeletal protein, protein 4.1B and p55, and sodium bicarbonate cotransporter 1 in mouse renal S1-S2 proximal tubules.2007

    • Author(s)
      Terada, N.
    • Journal Title

      J. Histochem. Cytochem. 55

      Pages: 1199-1206

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of endosomal cargo sorting by deubiquitinating enzymes.2007

    • Author(s)
      Komada, M.
    • Journal Title

      Research Advances in Biological Chemistry

      Pages: 27-38

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 神経繊維ランビエ絞輪部近傍の軸索構築に関するタンパク質間相互作用2007

    • Author(s)
      寺田 信生
    • Journal Title

      生体の科学 58

      Pages: 476-477

    • Related Report
      2007 Annual Research Report
  • [Presentation] LRRK1 はESCRT-0複合体と相互作用することでEGFR細胞内トラフィックを制御している2008

    • Author(s)
      花房洋
    • Organizer
      日本生化学会/日本分子生物学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] AMSHファミリーによるK63結合型ポリユビキチン鎖特異的な切断活性の構造的基盤2008

    • Author(s)
      佐藤裕介
    • Organizer
      日本生化学会/日本分子生物学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] AMSHファミリーによるK63結合型ポリュビキチン鎖特異的な切断活性の構造的基盤2008

    • Author(s)
      佐藤裕介
    • Organizer
      日本生化学会/日本分子生物学会合同大会BMB2008
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] LRRK1はESCRT-O複合体と相互作用することでEGFR細胞内トラフィックを制御している2008

    • Author(s)
      花房洋
    • Organizer
      日本生化学会/日本分子生物学会合同大会BMB2008
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] 脱ユビキチン化酵素USP36による核小体機能の制御2008

    • Author(s)
      遠藤彬則
    • Organizer
      日本生化学会/日本分子生物学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-11
    • Related Report
      2008 Final Research Report
  • [Presentation] 脱ュビキチン化酵素USP36による核小体機能の制御2008

    • Author(s)
      遠藤彬則
    • Organizer
      日本生化学会/日本分子生物学会合同大会BMB2008
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] 中央部紡錘体における時空間的に制御されたタンパク質ユビキチン化2008

    • Author(s)
      駒田雅之
    • Organizer
      日本生化学会/日本分子生物学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-10
    • Related Report
      2008 Final Research Report
  • [Presentation] 中央部紡錘体における時空間的に制御されたタンパク質ュビキチン化2008

    • Author(s)
      駒田雅之
    • Organizer
      日本生化学会/日本分子生物学会合同大会BMB2008
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] キイロショウジョウバエを用いた脱ユビキチン化酵素UBPYの機能解析2008

    • Author(s)
      向井明子
    • Organizer
      第60回日本細胞生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2008-07-01
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] エンドソームと細胞質分裂の接点-ユビキチン化と脱ユビキチン化による調節2008

    • Author(s)
      駒田雅之
    • Organizer
      第60回日本細胞生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2008-07-01
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] エンドソームで働く脱ユビキチン化酵素UBPYの活性調節とその意義2007

    • Author(s)
      駒田 雅之
    • Organizer
      日本生化学会/日本分子生物学会合同大会BMB2007・ワークショップ
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-15
    • Related Report
      2007 Annual Research Report
  • [Presentation] 脱ユビキチン化酵素USP36の機能解析2007

    • Author(s)
      遠藤 彬則
    • Organizer
      日本生化学会/日本分子生物学会合同大会BMB2007
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-14
    • Related Report
      2007 Annual Research Report
  • [Presentation] Disulfide bond formation plays a crucial role in protein stability of human ABCG22007

    • Author(s)
      若林 香菜子
    • Organizer
      日本生化学会/日本分子生物学会合同大会BMB2007
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-14
    • Related Report
      2007 Annual Research Report
  • [Presentation] キイロショウジョウバエにおける脱ユビキチン化酵素UBPYの機能解析2007

    • Author(s)
      向井 明子
    • Organizer
      日本生化学会/日本分子生物学会合同大会BMB2007
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] ユビキチン化と脱ユビキチン化によるABCG2の発現レベル調節2007

    • Author(s)
      山口 徳之
    • Organizer
      トランスポーター研究会 第1回関東部会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] Activation of an endosomal deubiquitinating enzyme in M phase2007

    • Author(s)
      駒田 雅之
    • Organizer
      国際シンポジウム"メンブレントラフィック"
    • Place of Presentation
      淡路島
    • Year and Date
      2007-11-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] ユビキチン化/脱ユビキチン化による受容体ダウンレギュレーションの調節2007

    • Author(s)
      駒田 雅之
    • Organizer
      第2回トランスポーター研究会・特別講演
    • Place of Presentation
      東京
    • Year and Date
      2007-06-09
    • Related Report
      2007 Annual Research Report
  • [Presentation] Endosome-associated deubiquitinating enzyme UBPY is regulated by 14-3-3 proteins in the M phase2007

    • Author(s)
      駒田 雅之
    • Organizer
      第59回日本細胞生物学会・ワークショップ
    • Place of Presentation
      福岡
    • Year and Date
      2007-05-30
    • Related Report
      2007 Annual Research Report
  • [Book] バイオ系のための基礎化学問題集2008

    • Author(s)
      三原 久和
    • Publisher
      講談社サイエンティフィク
    • Related Report
      2007 Annual Research Report
  • [Book] トコトンやさしいタンパク質の本2007

    • Author(s)
      有坂 文雄
    • Publisher
      日刊工業新聞社
    • Related Report
      2007 Annual Research Report
  • [Book] バイオ研究のフロンティア3-医療に向けた先端バイオ科学

    • Author(s)
      駒田雅之
    • Publisher
      増殖因子受容体の分解制御と制癌(印刷中)
    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.kitamura-komada-lab.bio.titech.ac.jp/

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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