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Anterior neural development requires the matrix protein Del1, which attenuates intracellular Wnt signaling

Research Project

Project/Area Number 19570192
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

INOMATA Hidehiko (2008)  The Institute of Physical and Chemical Research, 細胞分化・器官発生研究グループ, 研究員 (60372166)

笹井 紀明 (2007)  The Institute of Physical and Chemical Research, 細胞分化・器官発生研究グループ, 研究員 (80391960)

Co-Investigator(Kenkyū-buntansha) 猪股 秀彦  独立行政法人理化学研究所, 1細胞分化・器醗生研究グルーブ, 研究員 (60372166)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsアフリカツメガエル / 初期胚 / パターン形成 / BMP / 細胞外マトリックス / Wnt
Research Abstract

代表研究者らはアフリカツメガエルを用いて、分泌蛋白質Del1が初期胚の神経発生における前後軸の制御に必須の因子であることを見いだした。Del1の機能阻害では、神経の頭側の発生が抑制され、後方化した胚が発生する。一方、機能亢進実験により頭部形成が促進された。Del1がこれらの表現系を示す分子メカニズムを明らかにするために下流のシグナル解析を行ったところ、Del1はWntシグナルを抑制していることが判明した。レポーターアッセイを用いてDel1のWntシグナルに対する作用点を解析したところ、転写因子であるTcfもDel1の過剰発現により抑制された。また、タンパクレベルでの解析から、Del1は細胞外に分泌されたのち、細胞周辺に蓄積する細胞外マトリックスとして機能していることが示された。Del1は頭側に細胞外マトリックスを形成することにより、頭部抑制因子Wntシグナルから頭部を隔離し、神経発生における前後軸のパターン形成に関与している可能性が高い。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (10 results)

All 2008 2007

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (2 results)

  • [Journal Article] Ectodermal Factor Restricts Mesoderm Differentiation by Inhibiting p532008

    • Author(s)
      Sasai, N., Yakura, R,, Kamiya, D., Nakazawa, Y. and Sasai, Y
    • Journal Title

      Cell 133

      Pages: 878-890

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Robust Stability of the Embryonic Dorsal Axial Pattern Requires ONT1, a Secreted Scaffold for Chordin Degradation2008

    • Author(s)
      Inomata, H., Haraguchi, T. and Sasai, Y
    • Journal Title

      Cell 134

      Pages: 854-865

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Robust Stability of the Embryonic Dorsal Axial Pattern Requires ONT1, a Secreted Scaffold for Chordin Degradation2008

    • Author(s)
      Hidehiko Inomata
    • Journal Title

      Cell 134

      Pages: 854-865

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ectodermal Factor Restricts Mesoderm Differentiation by Inhibiting p532008

    • Author(s)
      Noriaki Sasai
    • Journal Title

      Cell 133

      Pages: 878-890

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The secreted EGF-Discoidin factor xDel1 is essential for dorsal development of the Xenopus embryo2007

    • Author(s)
      Arakawa, A., Matsuo-Takasaki, M., Takai, A., Inomata, H., Matsumura, M., Ikeya, M., Takahashi, K., Miyachi, Y., Sasai, N. and Sasai,Y
    • Journal Title

      Developmental Biology 306

      Pages: 160-169

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] XTsh3 is an essential enhancing factor of canonical Wnt signaling in Xenopus axial determination2007

    • Author(s)
      Onai, T., Matsuo-Takasaki, M., Inomata, H., Aramaki, T., Matsumura, M., Yakura, R., Sasai, N., and Sasai, Y
    • Journal Title

      EMBO J 26

      Pages: 2350-2360

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The secreted EGF-Discoidin factor xDel1 is essential for dorsal development of the Xenopus embryo.2007

    • Author(s)
      Akiko Arakawa
    • Journal Title

      Developmental Biology 306

      Pages: 160-169

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] XTsh3 is an essential enhancing factor of canonical Wnt signaling in Xenopusaxial determination.2007

    • Author(s)
      Takayuki Onai
    • Journal Title

      EMBO Journal 26

      Pages: 2350-2360

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Xenopus ONT1 is novel secreted pro-BMP factor that restricts Chordin in axial development2008

    • Author(s)
      猪股秀彦
    • Organizer
      41^<st> Annual Meeting for the Japanese Society of Developmental Biology
    • Place of Presentation
      徳島県郷土文化会館
    • Year and Date
      2008-05-28
    • Related Report
      2008 Final Research Report
  • [Presentation] Xenopus ONT1 is novel secreted pro-BMP factor that restricts Chordin in axial development2008

    • Author(s)
      猪股秀彦
    • Organizer
      41^<st> Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      徳島県郷土文化会館
    • Year and Date
      2008-05-28
    • Related Report
      2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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