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Identification and functional analysis of genes that affect the formation of endoderm and endodermal organs

Research Project

Project/Area Number 19570204
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionHiroshima University

Principal Investigator

KIKUCHI Yutaka  Hiroshima University, 大学院・理学研究科, 教授 (20286438)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsゼブラフィッシュ / 内胚葉性器官 / 器官形成 / 変異体 / 原因遺伝子 / 突然変異体
Research Abstract

本研究では、内胚葉性器官、特に消化器官形成の異常が顕著な新規変異体morendo(mor)とlegato(leg)変異体の解析を中心に研究を進め、mor変異体の原因遺伝子の同定に成功した。現在Morタンパク質の機能に関して研究を進めている段階である。また、leg変異体に関しては、原因遺伝子が含まれるゲノム領域の絞り込みに成功した。今後の解析により、原因遺伝子を同定することが出来ると考えている。
更に私は、内胚葉細胞が特異的に蛍光を発するトランスジェニックゼブラフィッシュTg(sox17 : EGFP)を用いることにより、原腸陥入期における内胚葉細胞の運動がケモカインシグナル(SDF1/Cxcr4シグナル)により制御されていることを明らかにし、論文発表(Mizoguchi et.al., 2008)を行った。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (18 results)

All 2008 2007 Other

All Journal Article (16 results) (of which Peer Reviewed: 11 results) Presentation (2 results)

  • [Journal Article] Sdf1/Cxcr4 signaling controls the dorsal migration of endodermal cells during zebrafish gastrulation2008

    • Author(s)
      Mizoguchi, T., Verkade, H., Heath, J., Kuroiwa, A. and Kikuchi Kikuchi, Y.
    • Journal Title

      Development 135

      Pages: 2521-2529

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Translational enhancement of recombinant protein synthesis in transgenic silkworms by a 5'- untranslated region of polyhedrin gene of Bombyx mori Nucleopolyhedrovirus2008

    • Author(s)
      Iizuka, M., Tomita, M., Shimizu, K., Kikuchi, Y. and Yoshizato, K.
    • Journal Title

      Journal of Bioscience and Bioengineering 105

      Pages: 595-603

    • NAID

      110006792535

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of nucleoplasmin 3 as a histone-binding protein in embryonic stem cells2008

    • Author(s)
      Motoi, N., Suzuki, K., Hirota, R., Johnson, P., Oofusa, K., Kikuchi, Y. and Yoshizato, K.
    • Journal Title

      Development Growth & Differentiation 50

      Pages: 307-320

    • NAID

      10021096598

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Extensive expansion and diversification of the chemokine gene family in zebrafish : identification of a novel chemokine subfamily CX2008

    • Author(s)
      Nomiyama, H., Hieshima, K., Osada, N., Kato-Unoki, Y., Otsuka-Ono, K., Takegawa, S., Izawa, T., Yoshizawa, A., Kikuchi, Y. Y., Tanase, S., Miura, R., Kusuda, J., Nakao, M., and Yoshie, O.
    • Journal Title

      BMC Genomics 9

      Pages: 222-222

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mtx2 directs zebrafish morphogenetic movements during epiboly by regulating microfilament formation2008

    • Author(s)
      Wilkins, S., Yoong, S., Verkade, H., Mizoguchi, T., Plowman, S., Hancock, J., Kikuchi, Y. Y., Heath, J. and Perkins, A.
    • Journal Title

      Developmental Biology 314

      Pages: 12-22

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 内胚葉分化にかかわるシグナル、転写因子群の機能2008

    • Author(s)
      溝口貴正, 菊池裕
    • Journal Title

      内分泌・糖尿病科 26

      Pages: 106-114

    • Related Report
      2008 Final Research Report
  • [Journal Article] Sdf1/Cxcr4シグナルはゼブラフィッシュの原腸陥入期において内胚葉細胞の背側への移動を制御する2008

    • Author(s)
      溝口貴正, 菊池裕
    • Journal Title

      細胞工学 27

      Pages: 1160-1161

    • Related Report
      2008 Final Research Report
  • [Journal Article] Sdfl/Cxcr4 signaling controls the dorsal migration of endodermal cells during zebrafish gastrulation.2008

    • Author(s)
      Mizoguchi, T., Verkade, H., Heath, J., Kuroiwa, A., Kikuchi. Y.
    • Journal Title

      Development 135

      Pages: 2521-2529

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sdfl/Cxcr4シグナルはゼブラフィッシュの原腸陥入期において内胚葉細胞の背側への移動を制御する2008

    • Author(s)
      溝口貴正, 菊池 裕
    • Journal Title

      細胞工学 27

      Pages: 1160-1161

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Mtx2 directs zebrafish morphogenetic movements during epiboly byregulating microfilament formation.2008

    • Author(s)
      Wilkins, S.J., Yoong, S., Verkade, H., Mizoguchi, T., Plowman, S.J., Hancock, J.F., Kikuchi. Y., Heath, J.K. and Perkins, A.C.
    • Journal Title

      Developmental Biology 314

      Pages: 12-22

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of nucleoplasmin 3 as a histone-binding protein in embryonic stem cells.2008

    • Author(s)
      Motoi, N., Suzuki, K., Hirota, R., Johnson, P., Oofusa, K., Kikuchi, Y. and Yoshizato, K.
    • Journal Title

      Developmental Growth & Differentiation (in press)

    • NAID

      10021096598

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Translational enhancement of recombinant protein synthesis in transgenic silkworms by a 5'-untranslated region of polyhedrin gene of Bombyx mori Nucleopolyhedrovirus.2008

    • Author(s)
      Iizuka, M., Tomita, M., Shimizu, K., Kikuchi, Y. and Yoshizato, K.
    • Journal Title

      Journal of Bioscience and Bioengineering (in press)

    • NAID

      110006792535

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Initial specification of the epibranchial placode in zebrafish embryos depends on the fibroblast growth factor signal2007

    • Author(s)
      Nikaido, M., Doi, K., Shimizu, T., Hibi, M., Kikuchi, Y. and Yamasu, K.
    • Journal Title

      Developmental Dynamics 236

      Pages: 564-571

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 特集"内胚葉分化の分子メカニズム"(編集 ; 菊池裕)序論-器官形成から再生を目指して-2007

    • Author(s)
      菊池裕
    • Journal Title

      蛋白質核酸酵素 52

      Pages: 101-104

    • Related Report
      2008 Final Research Report
  • [Journal Article] 脊椎動物における内胚葉形成の分子機構2007

    • Author(s)
      溝口貴正, 菊池裕
    • Journal Title

      蛋白核酸酵素 52

      Pages: 105-111

    • Related Report
      2008 Final Research Report
  • [Journal Article] Cyp26 enzymes function in endoderm to regulate pancreatic field size.

    • Author(s)
      Kinkel, M. D., Sefton, E. M., Kikuchi. Y., Mizoguchi, T., Andrea B. Ward, A. B., Prince, V. E.
    • Journal Title

      Proc. Natl. Acad. Sci. USA (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] ゼブラフィッシュ原腸陥入期における内胚葉細胞の移動機構2007

    • Author(s)
      溝口貴正, 黒岩厚, 菊池裕
    • Organizer
      第30回日本分子生物学会ワークショップ招待講演
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2007-12-14
    • Related Report
      2008 Final Research Report
  • [Presentation] ゼブラフィッシュ原腸陥入期における内胚葉細胞の移動機構2007

    • Author(s)
      菊池 裕
    • Organizer
      日本分子生物学会(ワークショップ)
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2007-12-14
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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