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Study of regulation mechanisms in the cell cycle coordinated with cell differentiation with translucent blastomeres of the frog, Xenopus laevis

Research Project

Project/Area Number 19570207
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionYamaguchi University

Principal Investigator

IWAO Yasuhiro  Yamaguchi University, 大学院・医学系研究科, 教授 (10144916)

Co-Investigator(Kenkyū-buntansha) UENO Shuichi  山口大学, 大学院・医学系研究科, 助教 (80363092)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords細胞分化 / 卵割 / 形態形成 / Caイオン / 発生・分化 / 動物 / 細胞周期 / 遺伝子発現 / 両生類 / PI3K / TOR / 体軸形成
Research Abstract

We studied the molecular mechanisms in cell cycle and cell differentiation with the efficient bio-imaging system of the translucent blastomeres of the frog, Xenopus laevis. The interactions of molecules necessary for DNA synthesis, such as DNA polymerase and cdc 45, was clarified with FRET methods. we found that the decrease in protein synthesis during MBT determined the onset of cell cycle elongation followed by cell differentiation. In addition, we investigated the role of Ca ions in the regulation of cell cycle and cell differentiation during early embryonic development.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (21 results)

All 2009 2008 2007 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (15 results) Remarks (4 results)

  • [Journal Article] The Sperm-surface glycoprotein, SGP, is necessary for fertilization in the frog, Xenopus laevis.2009

    • Author(s)
      Nagai K, Ishida T, Hashimoto T, Harada Y, Ueno S, Ueda Y, Kubo H, Iwao Y
    • Journal Title

      Development, Growth & Differentiation 51

      Pages: 499-510

    • NAID

      10025376389

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Sperm-surface glycoprotein, SGP, is necessary for fertilization in the frog, Xenopus laevis2009

    • Author(s)
      Keita Nagai
    • Journal Title

      Development, Growth & Differentiation 51

      Pages: 499-510

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 両生類ツメガエルの初期発生におけるクエン酸合成酵素の役割2009

    • Author(s)
      桑名貴幸
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] ツメガエル初期胚における細胞周期位相のイメージング2009

    • Author(s)
      山口壮太、上野秀一、岩尾康宏
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-10
    • Related Report
      2009 Final Research Report
  • [Presentation] ツメガエル初期胚における細胞周期位相のイメージング2009

    • Author(s)
      山口壮太
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] アフリカツメガエルの初期発生におけるクエン酸合成酵素の機能2009

    • Author(s)
      桑名貴幸
    • Organizer
      第80回日本動物学会
    • Place of Presentation
      静岡県コンベンションアーツセンター グランシップ
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] 精子マトリックスメタロプロテアーゼ(MMP)のツメガエルの受精における役割2009

    • Author(s)
      志賀圭子
    • Organizer
      第80回日本動物学会
    • Place of Presentation
      静岡県コンベンションアーツセンター グランシップ
    • Year and Date
      2009-09-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 透明化割球を用いたツメガエル初期胚細胞周期位相のイメージング2008

    • Author(s)
      山口壮太、上野秀一、岩尾康宏
    • Organizer
      第31回日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2009 Final Research Report
  • [Presentation] PI3K-TOR経路はツメガエル初期胚における細胞周期伸長開始に関与する2008

    • Author(s)
      瀧水智代、上野秀一、岩尾康宏
    • Organizer
      第31回日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2009 Final Research Report
  • [Presentation] PI3K-TOR経路はツメガエル初期胚における細胞周期伸長開始に関与する2008

    • Author(s)
      瀧水智代
    • Organizer
      第31回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド(神戸)
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] ツメガエル受精に必要な精子マトリツクスメタロプロテアーゼ-2(MMP-2)の性質2008

    • Author(s)
      吉川智康
    • Organizer
      第31回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド(神戸)
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] I3K/TORシグナル経路はツメガエル初期胚での細胞周期伸長開始に関与する2008

    • Author(s)
      瀧水智代、上野秀一、岩尾康宏
    • Organizer
      第79回日本動物学会
    • Place of Presentation
      福岡
    • Year and Date
      2008-09-06
    • Related Report
      2009 Final Research Report
  • [Presentation] PI3K/TORシグナル経路はツメガエル初期胚での細胞周期伸長開始に関与する2008

    • Author(s)
      瀧水智代
    • Organizer
      第79回日本動物学会
    • Place of Presentation
      福岡大学(博多)
    • Year and Date
      2008-09-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] PI3K/TOR pathway is involved in cell cycle elongation during Xenopus early embryogenesis2008

    • Author(s)
      瀧水智代、上野秀一、岩尾康宏
    • Organizer
      第41回日本発生生物学会(ISDB共催)
    • Place of Presentation
      徳島
    • Related Report
      2009 Final Research Report
  • [Presentation] PI3K/TORシグナル経路はツメガエル初期胚における細胞周期伸長に関与する2007

    • Author(s)
      瀧水智代、上野秀一、岩尾康宏
    • Organizer
      第30回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-11
    • Related Report
      2009 Final Research Report
  • [Presentation] PI3K/TORシグナル経路はツメガエル初期胚における細胞周期伸長に関与する2007

    • Author(s)
      瀧水 智代
    • Organizer
      第30回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2007-12-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] 正常細胞および形質転換細胞のpre-mitotic stageにおけるpaclitaxelの効果2007

    • Author(s)
      西尾 美則
    • Organizer
      第40回日本発生生物学会会合同大会第59回日本発生生物学
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2007-05-29
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://ds.cc.yamaguchi-u.ac.jp/~suenoscb/index.html

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://ds.ce.yamaguchi-u.ac.jp/~suenoscb/index.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://ds.cc.yamaguchi-u.ac.jp/~suenoscb/index.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://ds.cc.yamaguchi-u.ac.jp/~suenoscb/index.html

    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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