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Functional analysis of newly acquired genes that directed the evolution of chordates.

Research Project

Project/Area Number 19570220
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Evolutionary biology
Research InstitutionKochi University

Principal Investigator

FUJIWARA Shigeki  Kochi University, 教育研究部自然科学系, 教授 (40229068)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsレチノイン酸 / 発生・分化 / 脊索動物 / 進化 / 遺伝子 / Hox / Nodal / 遺伝子発現調節 / レチノイン酸受容体 / レチノイン酸合成酵素
Research Abstract

I analyzed the function of genes that were involved in the establishment of the chordate-specific body plan. Retinoic acid has been thought to function only in vertebrate embryos. In the present study, I demonstrated that retinoic acid contributed to organogenesis in the ascidian embryo, through the activation of the Hox1 gene. I also demonstrated that the growth factor, Nodal, was required for morphogenesis of the dorsal neural tube, which was a chordate-specific feature. The transcription factor, Cdx, was revealed to mediatethe Nodal signal and regulates neural tube formation.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (48 results)

All 2010 2009 2008 2007

All Journal Article (20 results) (of which Peer Reviewed: 20 results) Presentation (28 results)

  • [Journal Article] Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis2010

    • Author(s)
      Kaneko, N., Katsuyama, Y., Kawamura, K., Fujiwara, S
    • Journal Title

      Dev. Growth Difer vol.52

      Pages: 457-468

    • NAID

      10027565188

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of genes downstream of Nodal in the Ciona intestinalis embryo2010

    • Author(s)
      Mita, K., Koyanagi, R., Azumi, K., Sabau, S.V., Fujiwara, S
    • Journal Title

      Zool. Sci. vol 27

      Pages: 69-75

    • NAID

      40017358555

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of genes downstream of Nodal in the Ciona intestinalis embryo2010

    • Author(s)
      Mita, K.
    • Journal Title

      Zoological Science 27

      Pages: 69-75

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis2010

    • Author(s)
      Kaneko, N.
    • Journal Title

      Development, Growth & Differentiation 52(印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epidermal expression of Hox1 is directly activated by retinoic acid in the Ciona intestinalis embryo2009

    • Author(s)
      Kanda, M., Wada, H., Fujiwara, S
    • Journal Title

      Dev. Biol vol.35

      Pages: 454-463

    • NAID

      120001671558

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional regulation of the retinoic acid receptor in the dorsal midline epidermis in the Ciona intestinalis embryo2009

    • Author(s)
      Koyano, R., Ishida, S., Fujiwara, S
    • Journal Title

      Dev. Growth Dife vol.51

      Pages: 777-786

    • NAID

      10027562418

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism of DNA replication-dependent transcriptional activation of the acetylcholinesterase gene in the Ciona intestinalis embryo2009

    • Author(s)
      Kataoka, Y., Mishina, R., Fujiwara, S
    • Journal Title

      Dev. Growth Differ vol.51

      Pages: 841-850

    • NAID

      10027562643

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epidermal expression of Hox1 is directly activated by retinoic acid in the Ciona intestinalis embryo2009

    • Author(s)
      Kanda, M.
    • Journal Title

      Developmental Biology 335

      Pages: 454-463

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism of DNA replication-dependent transcriptional activation of the acetylcholinesterase gene in the Ciona intestinalis embryo2009

    • Author(s)
      Kataoka, Y.
    • Journal Title

      Development, Growth & Differentiation 51

      Pages: 841-850

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional regulation of the retinoic acid receptor in the dorsal midline epidermis in the Ciona intestinalis embryo2009

    • Author(s)
      Koyano, R.
    • Journal Title

      Development, Growth & Differentiation 51

      Pages: 777-786

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA interference by expresing short hairpin RNA in the Ciona intestinalis embryo2008

    • Author(s)
      Nishiyama, A., Fujiwara, S
    • Journal Title

      Dev. Growth Differ vol.50

      Pages: 521-529

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multipotent epithelial cells in the process of regeneration and asexual reproduction in colonial tunicates2008

    • Author(s)
      Kawamura, K., Sugino, Y., Sunanaga, T., Fujiwara, S
    • Journal Title

      Dev. Growth Differ vol.50

      Pages: 1-11

    • NAID

      10020274014

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] RNA interference by expressing short hairpin RNA in the Ciona intestinalis embryo.2008

    • Author(s)
      Nishiyama, A.
    • Journal Title

      Dev. Growth Differ. 50

      Pages: 521-529

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multipotent epithelial cells in the process of regeneration and asexual reproduction in colonial tunicates.2008

    • Author(s)
      Kawamura, K.
    • Journal Title

      Dev. Growth Differ. 50

      Pages: 1-11

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multipotent epithelial cells in the process of regeneration and asexual reproduction in colonial tunicates2008

    • Author(s)
      Kawamura, K.
    • Journal Title

      Develoment, Growth and Differentiation 50

      Pages: 1-11

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA interference by expressing short hairpin RNA in the Ciona intestinalis embryo2008

    • Author(s)
      Nishiyama, A.
    • Journal Title

      Develoment, Growth and Differentiation 50(印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nodal regulates neural tube formation in the Ciona intestinalis embryo2007

    • Author(s)
      Mita, K., Fujiwara, S
    • Journal Title

      Dev. Genes Evol vol.217

      Pages: 593-601

    • NAID

      120001073510

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene expresion profile during the life cycle of the urochordate Ciona intestinalis2007

    • Author(s)
      Azumi, K., Sabau, S.V., Fujie, M., Usami, T., Koyanagi, R., Kawashima, T., Fujiwara, S., Ogasawara, M., Satake, M., Nonaka, M., Wang, H-G., Satou, Y., Satoh, N
    • Journal Title

      Dev. Biol vol.308

      Pages: 572-582

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression profile during the life cycle of the urochordate Ciona intestinalis2007

    • Author(s)
      Azumi, K.
    • Journal Title

      Developmental Biology 308

      Pages: 572-582

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nodal regulates neural tube formation in the Ciona intestinalis embryo2007

    • Author(s)
      Mita, K.
    • Journal Title

      Developmelnt, Genes and Evolution 217

      Pages: 593-601

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Transcriptional regulation of Hox1 by retinoic acid in the Ciona intestinalis embryo2009

    • Author(s)
      Ikeda, T., Fujiwara, S., Kanda, M
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-11
    • Related Report
      2009 Final Research Report
  • [Presentation] Transcriptional regulation of Hox1 by retinoic acid in the Ciona intestinalis embryo2009

    • Author(s)
      Ikeda, T.
    • Organizer
      日本分子生物学会・第32回年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] カタユウレイボヤ胚におけるアセチルコリンエステラーゼ遺伝子のDNA複製回数依存的な転写調節の仕組み2009

    • Author(s)
      三科亮
    • Organizer
      日本分子生物学会・第32回年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] カタユウレイボヤ胚の背側正中線表皮におけるチノン酸受容体遺伝子の転写調節2009

    • Author(s)
      石田聡美・小谷野竜介・藤原滋樹
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡
    • Year and Date
      2009-09-17
    • Related Report
      2009 Final Research Report
  • [Presentation] カタユウレイボヤ胚の背側正中線表皮におけるレチノイン酸受容体遺伝子の転写調節2009

    • Author(s)
      石田聡美
    • Organizer
      日本動物学会・第80回大会
    • Place of Presentation
      グランシップ(静岡県)
    • Year and Date
      2009-09-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis2009

    • Author(s)
      Kaneko, N., Katsuyama, Y., Kawamura, K., Fujiwara, S
    • Organizer
      The 5th International Tunicate Meeting
    • Place of Presentation
      沖縄
    • Year and Date
      2009-06-24
    • Related Report
      2009 Final Research Report
  • [Presentation] Epidermal expression of Hox1 is directly activated by retinoic acid in the Ciona intestinalis embryo2009

    • Author(s)
      Kanda, M., Wada, H., Fujiwara, S
    • Organizer
      The 5th International Tunicate Meting
    • Place of Presentation
      沖縄
    • Year and Date
      2009-06-24
    • Related Report
      2009 Final Research Report
  • [Presentation] Large scale analysis of Nodal-regulated genes in the Ciona intestinalis embryo2009

    • Author(s)
      Mita, K., Koyanagi, R., Sabau, S., Azumi, K., Fujiwara, S
    • Organizer
      The 5th International Tunicate Meeting
    • Place of Presentation
      沖縄
    • Year and Date
      2009-06-24
    • Related Report
      2009 Final Research Report
  • [Presentation] Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis2009

    • Author(s)
      Kaneko, N.
    • Organizer
      The 5th International Tunicate Meeting
    • Place of Presentation
      沖縄産業支援センター
    • Year and Date
      2009-06-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Transposon-mediated enhancer trap revealed the functions of Hox1 during and after metamorphosis2009

    • Author(s)
      Sasakura, Y., Kanda, M., Horie, T., Satoh, N., Fujiwara, S
    • Organizer
      The 5th International Tunicate Meting
    • Place of Presentation
      沖縄
    • Year and Date
      2009-06-22
    • Related Report
      2009 Final Research Report
  • [Presentation] Transposon-mediated enhancer trap revealed the functions of Hox1 during and after metamorphosis2009

    • Author(s)
      Sasakura, Y.
    • Organizer
      The 5th International Tunicate Meeting
    • Place of Presentation
      沖縄産業支援センター
    • Year and Date
      2009-06-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] レチノイン酸によるカタユウレイボヤHox1遺伝子の転写調節2009

    • Author(s)
      神田美幸
    • Organizer
      日本動物学会中四国支部会・第61回大会
    • Place of Presentation
      高知大学(高知県)
    • Year and Date
      2009-05-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] Epidermal expression of Hox1 is directly activated by retinoic acid in the Ciona intestinalis embryo2009

    • Author(s)
      神田美幸
    • Organizer
      The 5th International Tunicate Meeting
    • Place of Presentation
      沖縄産業支援センター
    • Related Report
      2009 Annual Research Report
  • [Presentation] Large scale analysis of Nodal-regulated genes in the Ciona intestinalis embryo2009

    • Author(s)
      Mita, K.
    • Organizer
      The 5th International Tunicate Meeting
    • Place of Presentation
      沖縄産業支援センター
    • Related Report
      2009 Annual Research Report
  • [Presentation] Role of RACK1 homologue in zooidal growth and reproduction of budding tunicates2009

    • Author(s)
      Tatzuke, Y.
    • Organizer
      The 5th International Tunicate Meeting
    • Place of Presentation
      沖縄産業支援センター
    • Related Report
      2009 Annual Research Report
  • [Presentation] Nodal signaling regulates neural tube formation in embryos of the ascidian Ciona intestinalis2008

    • Author(s)
      Mita, K.
    • Organizer
      Gradient & Signalling : from chemotaxis to developments
    • Place of Presentation
      沖縄先端大
    • Year and Date
      2008-11-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] ミサキマメイタボヤの再生におけるレチノイン酸の機能2008

    • Author(s)
      金子永基・川村和夫・藤原滋樹
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡
    • Year and Date
      2008-09-07
    • Related Report
      2009 Final Research Report
  • [Presentation] ミサキマメイタボヤの再生におけるレチノイン酸の機能2008

    • Author(s)
      金子永基
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡大学
    • Year and Date
      2008-09-07
    • Related Report
      2008 Annual Research Report
  • [Presentation] ミサキマメイタボヤにおけるmycの発現解析および機能解析2008

    • Author(s)
      礒崎崇臣
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡大学
    • Year and Date
      2008-09-07
    • Related Report
      2008 Annual Research Report
  • [Presentation] レチノイン酸によるカタユウレイボヤHox1遺伝子の転写調節2008

    • Author(s)
      神田美幸・和田洋・藤原滋樹
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡
    • Year and Date
      2008-09-05
    • Related Report
      2009 Final Research Report
  • [Presentation] レチノイン酸によるカタユウレイボヤHox1遺伝子の転写調節2008

    • Author(s)
      神田美幸
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡大学
    • Year and Date
      2008-09-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] Roles of Nodal signaling in the embryo of the ascidian Ciona intestinalis2008

    • Author(s)
      Mita, K., Koyanagi, R., Azumi, K., Fujiwara, S
    • Organizer
      The 41st Anual Meeting for the Japanese Society of Developmental Biologists (Jointly sponsored by the International Society of Developmental Biologsits
    • Place of Presentation
      徳島
    • Year and Date
      2008-05-28
    • Related Report
      2009 Final Research Report
  • [Presentation] Roles of Nodal signaling in the embryo of the ascidian Ciona intestinalis2008

    • Author(s)
      Mita, K.
    • Organizer
      日本発生生物学会(国際発生生物学会共催)
    • Place of Presentation
      徳島県郷土文化会館
    • Year and Date
      2008-05-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] カタユウレイボヤの神経管形成におけるNodalシグナル機能解析2007

    • Author(s)
      三田薫・藤原滋樹
    • Organizer
      第30回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-11
    • Related Report
      2009 Final Research Report
  • [Presentation] カタユウレイボヤ胚におけるNodalシグナル標的遺伝子のマイクロアレイ解析2007

    • Author(s)
      三田 薫
    • Organizer
      日本分子生物学会第30回年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] ホヤの無性生殖におけるホメオボックス遺伝子のcDNA単離と発現解析2007

    • Author(s)
      柏原 央人
    • Organizer
      日本分子生物学会第30回年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] ホヤ胚におけるDNA複製回数依存的な転写調節の仕組みを探る試み2007

    • Author(s)
      藤原 滋樹
    • Organizer
      第2回ホヤ研究集会
    • Place of Presentation
      下田東急ホテル
    • Year and Date
      2007-05-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] カタユウレイボヤの神経管形成におけるNodalシグナルの機能解析2007

    • Author(s)
      三佃 薫
    • Organizer
      第2回ホヤ研究集会
    • Place of Presentation
      下田東急ホテル
    • Year and Date
      2007-05-07
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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