Molecular evolution of hatching enzyme of ancient fishes

• Project/Area Number: 19570221
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: Sophia University
• Principal Investigator: 安増 茂樹 Sophia University, 理工学部, 教授 (00222357)
• Co-Investigator(Kenkyū-buntansha): 井内 一郎 上智大学, 理工学部, 教授 (10011694)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 分子進化 / 孵化酵素 / 卵膜分解機構 / 機能進化 / チョウザメ / タンパク質機能進化 / プロテアーゼ / 遺伝子進化 / 古代魚 / 硬骨魚類

Research Abstract

申請研究は、表題にある古代魚孵化腺細胞の発生進化学的研究に加え、卵膜分解機構の進化研究の2題を含んでいる。前者においはて、チョウザメより孵化液を調整して、孵化酵素の精製途中である。後者の卵膜分解機構の進化研究では、初期の硬骨魚類孵化酵素の卵膜分解系は、膨潤化酵素の単一酵素であったものが、進化過程で、膨潤卵膜分解酵素が出現して2つの酵素の効率良い卵膜分解系に進化したことが明らかとなった。

Research Products

• [Journal Article] Different hatching strategies in embryos of two species,pacific herring Clupea pallasii and Japanese anchovy Engraulis japonicus, that belong to the same order Clupeiformes, and their environmental adaptation (2009)
  • Author(s): Kawaguchi M, Fujita H, Yoshizaki N, Hiroi J, Okouchi H, Nagakura Y,Noda T, Watanabe S, Katayama S, Iwamuro S,Nishida M, Iuchi I, Yasumasu S
  • Journal Title: J.Exp. Zoology B 312 Pages: 95-107
  • Peer Reviewed
• [Journal Article] Different hatching strategies in embryos of two species, pacific herring Clupea pallasii and Japanese anchovy Engraulis japonicus, that belong to the same order Clupeiformes, and their environmental adaptation. (2009)
  • Author(s): Kawaguchi M, Fujita H, Yoshizaki N, Hiroi J, Okouchi H, Nagakura Y, Noda T, Watanabe S, Katayama S, Iwamuro S, Nishida M, Iuchi I, Yasumasu S
  • Journal Title: J Exp Zoolog B 312 Pages: 95-107
  • Peer Reviewed
• [Journal Article] Purification and characterization of zebrafish hatching enzyme - an evolutionary aspect of the mechanism of egg envelope digestion (2008)
  • Author(s): Sano K, Inohaya K, Kawaguchi M,YoshizakiN, Iuchi I, Yasumasu S
  • Journal Title: FEBS J 275 Pages: 5934-5946
  • Peer Reviewed
• [Journal Article] Hatching enzyme of the ovoviviparous black rockfish Sebastes schlegeliienvironmental adaptation of the hatching enzyme and evolutionary aspects of formationof the pseudogene. (2008)
  • Author(s): Kawaguchi M, Nakagawa M, Noda T, Yoshizaki N, Hiroi J, Nishida M, Iuchi I, Yasumasu S.
  • Journal Title: FEBS J 275 Pages: 2884-2898
  • Peer Reviewed
• [Journal Article] Purification and characterization of zebrafish hatching enzymean evolutionary aspect of the mechanism of egg envelope digestion. (2008)
  • Author(s): Sano K, Inohaya K, Kawaguchi M, Yoshizaki N, Iuchi I, Yasumasu S
  • Journal Title: FEBS J. 275 Pages: 5934-5946
  • Peer Reviewed
• [Journal Article] Hatching enzyme of the ovoviviparous black rockfish Sebastes schlegelii-environmental adaptation of the hatching enzyme and evolutionary aspects of formationof the pseudogene. (2008)
  • Author(s): Kawaguchi M, Nakagawa M, Noda T, Yoshizaki N, Hiroi J, Nishida M, Iuchi I, Yasumasu S.
  • Journal Title: FEBS J. 275 Pages: 2884-2898
  • Peer Reviewed
• [Journal Article] Analysis of the exon-intron structures of fish, amphibian, bird and mammalian hatching enzyme genes, with special reference to the intron loss evolution of hatching enzyme genes in Teleostei (2007)
  • Author(s): Kawaguchi M, Yasumasu S, Hiroi J, Naruse K, Suzuki T, Iuchi I
  • Journal Title: Gene 392 Pages: 77-88
  • Peer Reviewed
• [Journal Article] Analysis of the exon-intron structures of fish, amphibian, bird and mammalian hatching enzyme genes, with special reference to the intron loss evolution of hatching enzyme genes in Teleostei. (2007)
  • Author(s): Kawaguchi M, Yasumasu S, Hiroi J, Naruse K, Suzuki T, Iuchi I.
  • Journal Title: Gene. 392 Pages: 77-88
  • Peer Reviewed
• [Journal Article] Globin gene enhancer activity of a DNase-I hypersensitive site-40homolog in medaka,Oryzias latipes (2007)
  • Author(s): Maruyama K., Ishikawa Y., Yasumasu S., Iuchi I.
  • Journal Title: Zoolog Sci 24 Pages: 997-1004
  • NAID: 40016396313
  • Peer Reviewed
• [Journal Article] Analysis of the exon-intron structures of fish,amphibian,bird and mammalian hatching enzyme genes,with special reference to the intron loss evolution of hatching enzyme genes in Teleostei (2007)
  • Author(s): Kawaguchi M., Yasumasu S., Hiroi J., Naruse K., Suzuki T., Iuchi I.
  • Journal Title: Gene.2007 392 Pages: 77-88
  • Peer Reviewed
• [Journal Article] Cloning and expression of xP1-L,a new marker gene for larval surface mucous cells of tadpole stomach in Xenopus laevis (2007)
  • Author(s): Ikuzawa M., Yasumasu S., Kobayashi K., Iuchi I.
  • Journal Title: Gene Exp Patterns 8 Pages: 12-8
  • Peer Reviewed
• [Presentation] 硬骨魚類孵化酵素の卵膜分解機構における機能進化 (2008)
  • Author(s): 佐野香織, 川口眞理, 井内一郎, 安増茂樹
  • Organizer: 日本進化学会第10回大会
  • Place of Presentation: 東京大学
  • Year and Date: 2008-08-22
• [Presentation] 孵化酵素LCEの種特異的卵膜分解 (2008)
  • Author(s): 川口眞理, 清水昭男, 廣井準也, 井内一郎, 安増茂樹, 西田睦
  • Organizer: 日本進化学会第10回大会
  • Place of Presentation: 東京大学
  • Year and Date: 2008-08-22
• [Presentation] 孵化酵素LCE の種特異的卵膜分解 (2008)
  • Author(s): 川口眞理、清水昭男、廣井準也、井内一郎、安増茂樹、西田睦
  • Organizer: 日本進化学会第9回大会
  • Place of Presentation: 東京大学
• [Presentation] ウナギ孵化酵素の卵膜分解特異性から考察した卵膜分解機構の進化 (2008)
  • Author(s): 佐野香織川口眞理井内一郎安増茂樹
  • Organizer: 第79 回日本動物学会
  • Place of Presentation: 福岡大
• [Presentation] 硬骨魚類孵化酵素の卵膜分解機構における機能進化 (2008)
  • Author(s): 佐野香織、川口眞理、井内一郎、安増茂樹
  • Organizer: 日本進化学会第9回大会
  • Place of Presentation: 東京大
• [Presentation] ゼブラフィッシュの孵化酵素とその卵膜分解機構 (2007)
  • Author(s): 佐野香織安増茂樹猪早敬二吉崎範夫川口眞理井内一郎
  • Organizer: 第13 回小型魚類研究会
  • Place of Presentation: 東京大
• [Presentation] ウナギ孵化酵素の精製とその卵膜分解機構 (2007)
  • Author(s): 佐野香織安増茂樹吉川昌之川口眞理井内一郎
  • Organizer: 第78 回日本動物学会
  • Place of Presentation: 弘前大
• [Presentation] 卵胎生魚クロソイの孵化酵素LCE 遺伝子における偽遺伝子形成機過程 (2007)
  • Author(s): 川口眞理、安増茂樹、廣井準也、中川雅弘、野田勉、井内一郎
  • Organizer: 日本進化学会第9回大会
  • Place of Presentation: 京都大学
• [Presentation] 単一酵素系としてのゼブラフィッシュ胚の孵化酵素 (2007)
  • Author(s): 佐野香織安増茂樹猪早敬二吉崎範夫川口眞理井内一郎
  • Organizer: 第59 回日本動物学会・関東支部会
  • Place of Presentation: 首都大学
• [Presentation] ウナギ艀化酵素の精製とその卵膜分解機構 (2007)
  • Author(s): 佐野、安増、吉川、川口、井内
  • Organizer: 日本動物学会
  • Place of Presentation: 弘前大学