Oxygen carrying mechanism of hemoglobin : verification of trigger model by using proximal cavity mutant hemoglobin
Project/Area Number |
19579002
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biophysics
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Research Institution | Kanazawa University |
Principal Investigator |
AKI Yayoi Kanazawa University, 保健学系, 助教 (00452110)
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Project Period (FY) |
2007 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥3,720,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2007: ¥1,900,000 (Direct Cost: ¥1,900,000)
|
Keywords | ヘモグロビン / 酸素運搬機能 / 高次構造変化 / 引き金説 / 四次構造変化 |
Research Abstract |
Many people believe that the trigger of the quaternary structural transition of hemoglobin (Hb) is the displacement of the proximal His, which is the only amino acid binding directly to the heme iron (trigger model). I inspected the model by synthesizing the recombinant Hbs, in which the proximal His is replaced with Gly in the α or β subunit, and then analyzing the conformational change and the oxygen carrying mechanism of these Hbs. I have suggested that the shift of the proximal His in the α subunit is the trigger of the structural transition of Hb, whereas one of the β subunit is not the trigger because the conformational change is partially observed in the recombinant Hb without the β proximal His.
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Report
(6 results)
Research Products
(33 results)
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[Journal Article] Effect of reversed heme orientation on circular dichoroism and cooperative oxygen binding of human adult hemoglobin2008
Author(s)
Nagai, M., Nagai, Y., Aki, Y., Imai, K., Wada, Y., Nagatomo, S., Yamamoto, Y.
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Journal Title
Biochemistry 47
Pages: 517-525
Related Report
Peer Reviewed
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[Journal Article] Reduction in hemoglobin-oxygen affinity results in the improvement of exercise capacity in mice with chronic heart failure2008
Author(s)
Watanabe T, Takeda T, Omiya S, Hikoso S, Yamaguchi O, Nakano Y, Higuchi Y, Nakai A, Abe Y, Aki-Jin Y, Taniike M, Mizote I, Matsumura Y, Shimizu T, Nishida K, Imai K, Hori M, Shirasawa T, Otsu K.
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Journal Title
Journal of the American College of Cardiology 52
Pages: 779-786
Related Report
Peer Reviewed
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[Journal Article] Effect of reversed heme orientation on circular dichoroism and cooperative oxygen binding of human adult hemoglobin2008
Author(s)
Nagai, M., Nagai, Y., Aki, Y., Imai, K., Wada, Y., Nagatomo, S. & Yamamoto, Y.
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Journal Title
Biochemistry 47
Pages: 517-525
Related Report
Peer Reviewed
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[Journal Article] Oxygenation properties of extracellular giant hemoglobin from Oligobrachia mashikoi2007
Author(s)
Aki, Y., Nakagawa, T., Nagai, M., Sasayama, Y., Fukumori, Y., Imai, K.
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Journal Title
Biochemical and Biophysical Research Communications 360
Pages: 673-678
Related Report
Peer Reviewed
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[Journal Article] Oxygenation properties of extracellular giant hemoglobin from Oligobrachia mashikoi2007
Author(s)
Aki, Y., Nakagawa, T., Nagai, M., Sasayama, Y., Fukumori, Y. & Imai, K
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Journal Title
Biochemical and Bjophysical Research Communications 360
Pages: 673-678
Related Report
Peer Reviewed
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[Presentation] Circular dichoroism and oxygen binding properties of human adult hemoglobin with reversed heme2008
Author(s)
Nagai, M., Nagai, Y., Aki, Y., Imai, K., Nagatomo, S. & Yamamoto, Y.
Organizer
Joint Meeting of the Biophysical Society 52nd Annual Meeting & 16th Ihternational Biophysics Congress
Place of Presentation
Long Beach (California)
Year and Date
2008-02-05
Related Report
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