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Oxygen carrying mechanism of hemoglobin : verification of trigger model by using proximal cavity mutant hemoglobin

Research Project

Project/Area Number 19579002
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionKanazawa University

Principal Investigator

AKI Yayoi  Kanazawa University, 保健学系, 助教 (00452110)

Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,720,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2007: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsヘモグロビン / 酸素運搬機能 / 高次構造変化 / 引き金説 / 四次構造変化
Research Abstract

Many people believe that the trigger of the quaternary structural transition of hemoglobin (Hb) is the displacement of the proximal His, which is the only amino acid binding directly to the heme iron (trigger model). I inspected the model by synthesizing the recombinant Hbs, in which the proximal His is replaced with Gly in the α or β subunit, and then analyzing the conformational change and the oxygen carrying mechanism of these Hbs. I have suggested that the shift of the proximal His in the α subunit is the trigger of the structural transition of Hb, whereas one of the β subunit is not the trigger because the conformational change is partially observed in the recombinant Hb without the β proximal His.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (33 results)

All 2010 2009 2008 2007 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (21 results) Remarks (3 results)

  • [Journal Article] Differences in coordination states of substituted tyrosine residues and quaternary structures among hemoglobin M probed by resonance Raman spectroscopy2010

    • Author(s)
      Aki Y., Nagai M., Nagai Y., Imai K., Aki M., Sato A., Kubo M., Nagatomo S., Kitagawa T.
    • Journal Title

      Journal of Biological Inorganic Chemistry 15

      Pages: 147-158

    • Related Report
      2010 Final Research Report 2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Differences in coordination states of substituted tyrosine residues and quaternary structures among hemoglobin M probed by resonance Raman spectroscopy2010

    • Author(s)
      Aki Y, Nagai M, Nagai Y, Imai K, Aki M, Sato A, Kubo M, Nagatomo S, Kitagawa T.
    • Journal Title

      Journal of Biological Inorganic Chemistry 15

      Pages: 147-158

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of reversed heme orientation on circular dichoroism and cooperative oxygen binding of human adult hemoglobin2008

    • Author(s)
      Nagai, M., Nagai, Y., Aki, Y., Imai, K., Wada, Y., Nagatomo, S., Yamamoto, Y.
    • Journal Title

      Biochemistry 47

      Pages: 517-525

    • Related Report
      2010 Final Research Report 2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Reduction in hemoglobin-oxygen affinity results in the improvement of exercise capacity in mice with chronic heart failure2008

    • Author(s)
      Watanabe T, Takeda T, Omiya S, Hikoso S, Yamaguchi O, Nakano Y, Higuchi Y, Nakai A, Abe Y, Aki-Jin Y, Taniike M, Mizote I, Matsumura Y, Shimizu T, Nishida K, Imai K, Hori M, Shirasawa T, Otsu K.
    • Journal Title

      Journal of the American College of Cardiology 52

      Pages: 779-786

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of reversed heme orientation on circular dichoroism and cooperative oxygen binding of human adult hemoglobin2008

    • Author(s)
      Nagai, M., Nagai, Y., Aki, Y., Imai, K., Wada, Y., Nagatomo, S. & Yamamoto, Y.
    • Journal Title

      Biochemistry 47

      Pages: 517-525

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oxygenation properties of extracellular giant hemoglobin from Oligobrachia mashikoi2007

    • Author(s)
      Aki, Y., Nakagawa, T., Nagai, M., Sasayama, Y., Fukumori, Y., Imai, K.
    • Journal Title

      Biochemical and Biophysical Research Communications 360

      Pages: 673-678

    • Related Report
      2010 Final Research Report 2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Changes of near-UV CD spectra of human hemoglobin upon the R->T quaternary structure transition2007

    • Author(s)
      Aki-Jin Y., Nagai Y., Imai K., Nagai M.
    • Journal Title

      ACS Symposium Book on "New Approaches in Biomedical Spectroscopy" 963

      Pages: 297-311

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oxygenation properties of extracellular giant hemoglobin from Oligobrachia mashikoi2007

    • Author(s)
      Aki, Y., Nakagawa, T., Nagai, M., Sasayama, Y., Fukumori, Y. & Imai, K
    • Journal Title

      Biochemical and Bjophysical Research Communications 360

      Pages: 673-678

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes of near-UV circular dichroism spectra of human hemoglobin upon the R→T quaternary structure transition2007

    • Author(s)
      Aki-Jin, Y., Nagai, Y., Imai, K. & Nagai, M
    • Journal Title

      ACS Symposium Series on New Approaches in Biomedical Spectroscopy 963

      Pages: 297-311

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Coordination states of proximal or distal tyrosine residue in Hemoglobins M and their quaternary structures probed by resonance Raman spectroscopy2009

    • Author(s)
      Aki Y., Nagai M., Imai K., Aki M., Kitagawa T.
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      アスティとくしま(徳島県)
    • Year and Date
      2009-10-30
    • Related Report
      2010 Final Research Report
  • [Presentation] Coordination states of proximal or distal tyrosine residue in Hemoglobins M and their quaternary structures probed by resonance Raman spectroscopy2009

    • Author(s)
      Aki Y., Nagai M., Imai K., Aki M., Kitagawa T.
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      アスティとくしま (徳島県)
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report 2009 Self-evaluation Report
  • [Presentation] Differences in coordination states of replaced tyrosine residues and quaternary structures among hemoglobins M probed by resonance Raman Spectroscopy14th International Conference on Biological2009

    • Author(s)
      長井幸史, 安藝弥生, 北川禎三
    • Organizer
      Inorganic Chemistry
    • Place of Presentation
      Nagoya Congress Center(愛知県)
    • Year and Date
      2009-07-26
    • Related Report
      2010 Final Research Report
  • [Presentation] Differences in coordination states of replaced tyrosine residues and quaternary structures among hemoglobins M probed by resonance Raman spectroscopy2009

    • Author(s)
      Nagai M, Aki Y, Kitagawa T.
    • Organizer
      14th International Conference on Biological, Inorganic Chemistry
    • Place of Presentation
      Nagoya Congress Center (愛知県)
    • Year and Date
      2009-07-26
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Differences in coordination states of replaced tyrosine residues and quaternary structures among hemoglobins M probed by resonance Raman spectroscopy2009

    • Author(s)
      長井雅子, 安藝弥生, 北川禎三
    • Organizer
      14^<th> International Conference on Biological Inorganic Chemistry
    • Place of Presentation
      Nagoya Congress Center (愛知県)
    • Year and Date
      2009-07-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヘモグロビンの3つのトリプトファン残基の環境とその酸素結合による変化 : 近紫外円二色性及び紫外共鳴ラマン分光による研究2009

    • Author(s)
      長井雅子, 大久保賢一, 安藝弥生, 長井幸史, 今井清博, 長友重紀, 北川禎三
    • Organizer
      第36回生体分子科学討論会
    • Place of Presentation
      北海道大学学術交流会館 (北海道)
    • Year and Date
      2009-06-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] Functional properties of mersalylmodified hemoglobin (2)2008

    • Author(s)
      Kuriyama, S., Aki, Y, Imai K.
    • Organizer
      第46回日本生物物理学会年会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2008-12-03
    • Related Report
      2010 Final Research Report
  • [Presentation] Functional properties of mersalyl-modified hemoglobin (2)2008

    • Author(s)
      Kuriyama, S., Aki, Y. Imal, K.
    • Organizer
      第46回日本生物物理学会牟会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2008-12-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] Environmental alterations of three tryptophan residues in human adult hemoglobin (Hb A) : A near-UV circular dichroism study2008

    • Author(s)
      Ohkubo, K., Aki, Y., Imai, K., Nagai, Y., Nagai, M.
    • Organizer
      第46回日本生物物理学会年会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2008-12-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] Environmental alterations of three tryptophan residues in human adult hemoglobin upon ligand binding2008

    • Author(s)
      小久保昭秀, 安藝弥生, 今井清博, 長井雅子, 長井幸史
    • Organizer
      XXI International Conference on Raman Spectroscopy ICORS 2008
    • Place of Presentation
      Uxbridge (United Kingdom)
    • Year and Date
      2008-08-17
    • Related Report
      2010 Final Research Report
  • [Presentation] Environmental alterations of three tryptophan residues in human adult hemoglobin upon ligand binding2008

    • Author(s)
      Ohkubo, K., Aki, Y., Imai, K., Nagai, Y., Nagai, M.
    • Organizer
      XXI International Conference on Raman Spectroscopy ICORS 2008
    • Place of Presentation
      Uxbridge (West London)
    • Year and Date
      2008-08-17
    • Related Report
      2008 Annual Research Report
  • [Presentation] 逆配向ヘムをもつヘモグロビンの酸素平衡機能と円二色性(CD)2008

    • Author(s)
      長井雅子、長井幸史、今井清博、安藝弥生、長友重紀、山本泰彦
    • Organizer
      第35回生体分子科学討論会
    • Place of Presentation
      兵庫県立大学(兵庫県)
    • Year and Date
      2008-06-26
    • Related Report
      2010 Final Research Report
  • [Presentation] 逆配向ヘムをもつヘモグロビンの酸素平衡機能と円二色性(CD)2008

    • Author(s)
      長井雅子, 長井幸史, 今井清博, 安藝弥生, 長友重紀, 山本泰彦
    • Organizer
      第35回生体分子科学討論会
    • Place of Presentation
      兵庫県立大学(姫路市)
    • Year and Date
      2008-06-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] Circular dichoroism and oxygen binding properties of human adult hemoglobin with reversed heme2008

    • Author(s)
      Nagai M., Nagai Y., Aki Y., Imai K., Nagatomo S., Yamamoto, Y.
    • Organizer
      Joint Meeting of the Biophysical Society 52nd Annual Meeting & 16th International Biophysics Congress
    • Place of Presentation
      Long Beach (USA)
    • Year and Date
      2008-02-05
    • Related Report
      2010 Final Research Report
  • [Presentation] Circular dichoroism and oxygen binding properties of human adult hemoglobin with reversed heme2008

    • Author(s)
      Nagai, M., Nagai, Y., Aki, Y., Imai, K., Nagatomo, S. & Yamamoto, Y.
    • Organizer
      Joint Meeting of the Biophysical Society 52nd Annual Meeting & 16th Ihternational Biophysics Congress
    • Place of Presentation
      Long Beach (California)
    • Year and Date
      2008-02-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] 有鬚動物マシコヒゲムシのもつ巨大ヘモグロビンの酸素運搬機能2007

    • Author(s)
      安藝弥生、中川太郎、福森義宏、長井雅子、今井清博
    • Organizer
      第45回日本生物物理学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2007-12-23
    • Related Report
      2010 Final Research Report
  • [Presentation] ヒトヘモグロビンにおける3つのトリプトファン残基の酸素結合にともなう変化:近紫外CDおよび紫外共鳴ラマン分光による研究2007

    • Author(s)
      長井雅子、長井幸史、小久保昭秀、川口裕貴、安藝弥生、今井清博、長友重紀、北川禎三
    • Organizer
      第45回日本生物物理学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2007-12-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 有鬚動物マシコヒゲムシのもつ巨大ヘモグロビンの酸素運搬機能2007

    • Author(s)
      安藝弥生、中川太郎、長井雅子、笹山雄一、福森義宏、今井清博
    • Organizer
      第45回日本生物物理学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2007-12-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] ヒトヘモグロビンにおける3つのトリプトファン残基の酸素結合にともなう変化:近紫外CDおよび紫外共鳴ラマン分光による研究2007

    • Author(s)
      長井雅子、長井幸史、小久保昭秀、川口裕貴、安藝弥生、今井清博、長友重紀、北川禎三
    • Organizer
      第45回日本生物物理学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2007-12-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] マシコヒゲムシ巨大ヘモグロビンの酸素結合特性2007

    • Author(s)
      安藝弥生、中川太郎、福森義宏、長井雅子、今井清博
    • Organizer
      第7回日本蛋白質科学会年会
    • Place of Presentation
      仙台国際センター(宮城県)
    • Year and Date
      2007-05-25
    • Related Report
      2010 Final Research Report
  • [Presentation] マシコヒゲムシ巨大ヘモグロビンの酸素結合特性2007

    • Author(s)
      安藝弥生、中川太郎、福森義宏、長井雅子、今井清博
    • Organizer
      第7回日本蛋白質科学会年会
    • Place of Presentation
      仙台国際センター(仙台市)
    • Year and Date
      2007-05-25
    • Related Report
      2007 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://kurt.kanazawa-u.ac.jp/souran_ku/info.php?teacher_id=881

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://kurt.kanazawa-u.ac.jp/souran_ku/info.php?teacher_id=881

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://kurt.kanazawa-u.ac.jp/souran_ku/info.php?teacher_id=881

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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