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Development of methods for genome identification and chromosome doubling in liliaceous ornamental plants

Research Project

Project/Area Number 19580024
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionNiigata University

Principal Investigator

OKAZAKI Keiichi  Niigata University, 自然科学系, 教授 (20270936)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsFISH / GISH / チューリップ / 染色体 / 笑気ガス
Research Abstract

倍数性および種間交雑育種法は園芸作物の育種として極めて重要である。そこで,本研究では,染色体倍加の新技術として,笑気ガス処理による花粉の染色体を倍加する方法や種間雑種の不稔性を回復する技術を開発した。また,ゲノムや染色体の同定を行うため,rDNAを用いたFISH解析やGISH解析により,雑種のゲノム構成を明らかにする技術を開発した。これらの技術は,ユリおよびチューリップの染色体同定に有効であるとともに,不稔性種間交雑種の稔性を回復させ交配母本とし利用できることを示した画期的な育種法を提示するものである。

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (20 results)

All 2009 2008 2007

All Journal Article (12 results) (of which Peer Reviewed: 6 results) Presentation (8 results)

  • [Journal Article] Diploid endosperm formation in Tulipa spp. and identification of a 1:1 maternaltopaternal genome ratio in endosperms of T. gesneriana2009

    • Author(s)
      H. Mizuochi, H. Matsuzaki, T. Moue, K. Okazaki
    • Journal Title

      L. Sex Plant Reprod 22

      Pages: 27-36

    • Related Report
      2009 Final Research Report
  • [Journal Article] Mechanism of action of nitrous oxide gas applied as a polyploidizing agent during meiosis in lilies2009

    • Author(s)
      S. Kitamura, M. Akutsu, K. Okazaki
    • Journal Title

      Sex. Plant Reprod 22

      Pages: 9-14

    • Related Report
      2009 Final Research Report
  • [Journal Article] Diploid endosperm formation in Tulipa spp. and identify-ication of a 1:1 maternal-to-paternal genome ratio in endosperms of T. gesneriana L.2009

    • Author(s)
      H. Mizuochi, H. Matsuzaki, T. Moue, K. Okazaki
    • Journal Title

      Sex Plant Reprod. 22

      Pages: 27-36

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism of action of nitrous oxide gas applied as a polyploidizing agent during meiosis in lilies2009

    • Author(s)
      S. Kitamura, M. Akutsu, K. Okazaki
    • Journal Title

      Sex Plant Reprod. 22

      Pages: 9-14

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of introgression of Tulipa fosteriana genome into Tulipa gesneriana using GISH and FISH2008

    • Author(s)
      A. Marasek, K. Okazaki
    • Journal Title

      Euphytica 160

      Pages: 217-230

    • Related Report
      2009 Final Research Report
  • [Journal Article] Analysis of introgression of Tulipa fosteriana genome into Tulipa gesneriana using GISH and FISH2008

    • Author(s)
      A. Marasek, K Okazaki
    • Journal Title

      Euphytica 160

      Pages: 217-230

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GISH Analysis of Hybrids Produced by Interspecific Hybridization between Tulipa gesneriana and T. fosteriana2007

    • Author(s)
      A. Marasek, K. Okazaki
    • Journal Title

      Acta Horticul 743

      Pages: 133-137

    • Related Report
      2009 Final Research Report
  • [Journal Article] Production of 2n pollen of Asiatic hybrid lilies by nitrous oxide treatment2007

    • Author(s)
      M. Akutsu, S. Kitamura, R. Toda, I. Miyajima, K. Okazaki
    • Journal Title

      Euphytica 155

      Pages: 143-152

    • Related Report
      2009 Final Research Report
  • [Journal Article] Molecular cloning of Tulipa fosteriana rDNA and subsequent FISH analysis yields cytogenetic organization of 5S rDNA and 45S rDNA in T. gesneriana and T. fosteriana2007

    • Author(s)
      H. Mizuochi1, A. Marasek, K. Okazaki
    • Journal Title

      Euphytica 155

      Pages: 235-248

    • Related Report
      2009 Final Research Report
  • [Journal Article] Molecular cloning of Tulipa fosteriana rDNA and subsequent FISH analysis yields cytogenetic organization of 5S rDNA and 45S rDNA in T. gesneriana and T. fosteriana2007

    • Author(s)
      H Mizuochi, A Marasek, and K Okazaki
    • Journal Title

      Euphytica 155

      Pages: 235-248

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of 2n pollen of Asiatic hybrid lilies by nitrous oxide treatment2007

    • Author(s)
      M Akutsu, S Kitamura, R Toda, I Miyajima and K Okazaki1
    • Journal Title

      Euphytica 155

      Pages: 143-152

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GISF analysis of hybrids produced by interspecific hybridization between Tulipa gesneriana and T. fosteriana2007

    • Author(s)
      A Marasek, and K Okazaki
    • Journal Title

      Acta Horticulturace 747

      Pages: 133-137

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 笑気ガス処理による不稔性ユリ種間雑種の稔性回復と得られた稔性回復花粉を用いた後代の育成2009

    • Author(s)
      岡崎桂一, 北村里美, 温井祥太郎, 日置智代, 大塚英昭, 三吉一光, 佐藤孝夫, 高取由佳, 高濱雅幹
    • Organizer
      園芸学会
    • Place of Presentation
      秋田大学
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] ユリにおける笑気ガス処理が染色体倍加花粉獲得に及ぼす影響2009

    • Author(s)
      高濱雅幹、黒鳥学、大友知、鈴木亮子、生方雅男、岡崎桂一
    • Organizer
      園芸学会
    • Place of Presentation
      秋田大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] 笑気ガス処理によるシンテッポウユリ倍数体の作出2009

    • Author(s)
      佐藤孝夫、佐藤友博、三吉一光、岡崎桂一
    • Organizer
      園芸学会
    • Place of Presentation
      秋田大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] 受精卵に対する新染色体倍加法(笑気ガス処理)によるアジアティックおよびオリエンタルユリの4倍性実生の育成2009

    • Author(s)
      大塚英昭、日置智代、温井祥太郎、北村里美、岡崎桂一
    • Organizer
      園芸学会
    • Place of Presentation
      秋田大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] Induction of 2n Gametes and 4n Embryo in Lilium(Lilium×formolongihort.)by Nitrous Oxide Gus Treatment2009

    • Author(s)
      Takao Sato, Kazumitsu Miyoshi, Tomohiro Sato, Keiichi Okazaki
    • Organizer
      23rd Eucarpia Symposium-Section omamentals
    • Place of Presentation
      オランダ
    • Related Report
      2009 Annual Research Report
  • [Presentation] ユリにおける染色体倍加花粉獲得のための笑気ガス処理技術の確立,(第二報)アジアティックハイブリッドユリおよびジンテッポウユリにおける花粉の第一減数分裂中期の笑気ガス処理2009

    • Author(s)
      高取由佳・宮崎雄太・大藪榮興・岡崎桂一
    • Organizer
      園芸学会九州支部第49回大会
    • Place of Presentation
      佐賀市
    • Related Report
      2009 Annual Research Report
  • [Presentation] 笑気ガス処理を用いた花粉母細胞の染色体倍加によるユリ種間雑種の稔性回復2007

    • Author(s)
      北村里美・岡崎桂一
    • Organizer
      日本育種学会
    • Place of Presentation
      山形大学
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] Production of 2n pollne by chromosome doubling in pre-meiotic cell division of PMC with nitrous oxide treatment2007

    • Author(s)
      S kitamura, K Okazaki
    • Organizer
      II International symp on Food Security, Agric. Devel. & Envr. Conser in Souh. East Asia
    • Place of Presentation
      Bogor, Indonesia
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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