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Isolation of host factor genes involved in the multiplication of dianthvirus using non-destructive quantification system

Research Project

Project/Area Number 19580046
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant pathology
Research InstitutionKyoto University

Principal Investigator

KAIDO Masanori  Kyoto University, 大学院農学研究科, 助教 (20314247)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords植物ウイルス / 細胞間移行 / 小胞体膜 / 細胞内局在性 / 宿主因子 / 移行タンパク質 / RNAウイルス / 複製酵素
Research Abstract

マメ科植物の重要病害ウイルスであるRed clover necrotic mosaic virus(RCNMV)の細胞間移行過程において中心的な役割を演ずる移行タンパク質(MP)の細胞内での局在性とその機構を詳細に調べた。MPは自身のC末端68アミノ酸を通じて小胞体膜にRCNMV複製酵素と共局在し、それはRCNMVゲノム複製には影響しないが、細胞間移行にとって必須の過程であることを明らかにした。さらにRCNMV MPと細胞内で相互作用する宿主因子タンパク質を免疫沈降法によって精製できることを明らかにした。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (49 results)

All 2009 2008 2007

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (36 results)

  • [Journal Article] Genetic analysis of a host determination mechanism of bromoviruses in Arabidopsis thaliana2009

    • Author(s)
      K.Fujisaki, F. Iwahashi, M. Kaido, T. Okuno and K. Mise
    • Journal Title

      Virus Research 140

      Pages: 103-111

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic analysis of a host determination mechanism of brpmoviruses in Arabidonsis thaliana2009

    • Author(s)
      K. Fujisaki, F. Iwahashi, M. Kaido, T. Okuno, K. Mise
    • Journal Title

      Virus Research 140

      Pages: 103-111

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 7番目(2008) A viral noncoding RNA generated by cis-element-mediated protection against 5' 3' RNA decay represses both cap-independent and cap-dependent translation2008

    • Author(s)
      H. Iwakawa 他8名
    • Journal Title

      Journal of Virology 82

      Pages: 10162-10174

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Okuno (2008) cis-Preferential requirement of a-1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA12008

    • Author(s)
      K. Okamoto, H. Nagano, H. Iwakawa, H. Mizumoto, A. Takeda, M. Kaido, K. Mise and T
    • Journal Title

      Virology 375

      Pages: 205-212

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A viral noncoding RNA generated by cis-element-mediated protection against 5'→3' RNA decay represses both cap-independent and cap-dependent translation2008

    • Author(s)
      H. Iwakawa, H. Mizumoto, H. Nagano, Y. Imoto, K. Takigawa, S. Sarawaneeyaruk, M. Kaido, K. Mise, T. Okuno
    • Journal Title

      Journal of Virology 82

      Pages: 10162-10174

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] cis-Preferential requirement of a -1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA12008

    • Author(s)
      K. Okamoto, H. Nagano, H. Iwakawa, H. Mizumoto, A. Takeda, M. Kaido, K. Mise, T. Okuno
    • Journal Title

      Virology 375

      Pages: 205-212

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cis-preferential requirement of a -1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA12008

    • Author(s)
      Okamoto, et. al.
    • Journal Title

      Virology 380(In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Okuno (2007) cis-Acting core RNA elements required for negative-strand RNA synthesis and cap-independent translation are separated in the 3'-untranslated region of Red clover necrotic mosaic virus RNA12007

    • Author(s)
      H. Iwakawa, M. Kaido, K. Mise and T
    • Journal Title

      Virology 369

      Pages: 168-181

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation and onteraction of the movement and coat proteins of Brome mosaic virus in infected barley protoplasts2007

    • Author(s)
      N. Akamatsu, A.Takeda, M. Kishimoto, M. Kaido, T. Okuno and K. Mise
    • Journal Title

      Archives of Virology 152

      Pages: 2087-2093

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 1番目 (2007) Downregulation of the NbNACa1 gene encoding a movement-protein-interacting protein reduces cell-to-cell movement of Brome mosaic virus in Nicotiana benthamiana2007

    • Author(s)
      M.Kaido 他9名
    • Journal Title

      Molecular Plant-Microbe Interactions 20

      Pages: 671-681

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Downregulation of the NbNACal gene encoding a movement protein-interacting protein reduces cell-to-cell movement of Brome mosaic virus in Nicotiana benthamiana2007

    • Author(s)
      Kaido, et. al.
    • Journal Title

      Molecular Plant-Microbe Interactions 20

      Pages: 671-681

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation and interaction of the movement and coat proteins of Brome mosaic virus in infected barley protoplasts2007

    • Author(s)
      Akamatsu, et. al.
    • Journal Title

      Archives of Virology 152

      Pages: 2087-2093

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cis-acting core RNA elements required for negative-strand RNA synthesis and cap-independent translation are separated in the 3'-untranslated region of Red clover necrotic mosaic virus RNA12007

    • Author(s)
      Iwakawa, et. al.
    • Journal Title

      Virology 369

      Pages: 168-181

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Red clover necrotic mosaic virus (RCNMV)移行タンパク質(MP)のER局在性は細胞間移行に影響する2009

    • Author(s)
      海道真典・築野靖子・三瀬和之・奥野哲郎
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形県山形市
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Host-deendent roles of the 5' untranslated region (UTR) of Red clover necrotic mosaic virus (RCNMV) RNA1 in cap-independent translation mediated by its 3'UTR2009

    • Author(s)
      S. Sarawaneeyaruk, H. Iwakawa, M. Kaido, K. Mise and T. Okuno
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形県山形市
    • Year and Date
      2009-03-27
    • Related Report
      2008 Final Research Report
  • [Presentation] Red clover necrotic mosaic virus RNA1の3'端非翻訳領域における -1フレームシフトに必要なシス因子の同定2009

    • Author(s)
      田島由理・岩川弘宙・海道真典・三瀬和之・奥野哲郎
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形県山形市
    • Year and Date
      2009-03-27
    • Related Report
      2008 Final Research Report
  • [Presentation] Strepto Tag法を用いたRed clover necrotic mosaic virus RNA2に存在する複製酵素リクルート因子の同定2009

    • Author(s)
      岩川弘宙・安夢楠・峯彰・海道真典・三瀬和之・奥野哲郎
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形県山形市
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Red clover necrotic mosaic virus複製酵素タンパク質が形成する複合体の性状解析2009

    • Author(s)
      峯彰・竹田篤史・海道真典・三瀬和之・奥野哲郎
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形県山形市
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Melandrium yellow fleck virus (MYFV) RNA3の5'非翻訳領域内に存在する短いopen reading frame (ORF)がウイルス感染に与える影響2009

    • Author(s)
      楢林大樹・海道真典・奥野哲郎・三瀬和之
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形県山形市
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Host-deendent roles of the 5'untranslated region (UTR) of Red clover necrotic mosaic virus(RCNMV) RNA1 in cap-independent translation mediated by its 3'UTR2009

    • Author(s)
      S. Sarawaneeyaruk, H. Iwakawa, M. Kaido, K. Mise, T. Okuno
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形県山形市
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] Red clover necrotic mosaic virus RNA1の3'端非翻訳領域における-1フレームシフトに必要なシス因子の同定2009

    • Author(s)
      田島由理, 岩川弘宙, 海道真典, 三瀬和之, 奥野哲郎
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形県山形市
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 分節ゲノムを持つダイアンソウイルスのRNA複製制御機構2008

    • Author(s)
      岩川弘宙・安夢楠・峯彰・海道真典・三瀬和之・奥野哲郎
    • Organizer
      第56回日本ウイルス学会学術集会
    • Place of Presentation
      岡山県岡山市
    • Year and Date
      2008-10-26
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 複製酵素タンパク質による膜画分へのRNAリクルートメントにおけるRed clover necrotic mosaic virusゲノムRNA2 3'UTRの役割2008

    • Author(s)
      峯彰・安夢楠・岩川弘宙・海道真典・三瀬和之・奥野哲郎
    • Organizer
      第56回日本ウイルス学会学術集会
    • Place of Presentation
      岡山県岡山市
    • Year and Date
      2008-10-26
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ER-targeting of Red clover necrotic mosaic virus movement protein is dependent on the viral RNA replication2008

    • Author(s)
      M. Kaido, Y. Tsuno, K. Mise and T. Okuno
    • Organizer
      XIV. International Congress of Virology
    • Place of Presentation
      Istanbul, Turkey
    • Year and Date
      2008-08-13
    • Related Report
      2008 Final Research Report
  • [Presentation] A viral non-coding RNA generated by cis-element-mediated protection against 5'→3' decay represses both cap-independent and dependent translation2008

    • Author(s)
      H. Iwakawa,H. Mizumoto, Y. Imoto, K. Takigawa, S. Sarawaneeyaruk, M. Kaido, K. Mise, T. Okuno
    • Organizer
      XIV. International Congress of Virology
    • Place of Presentation
      Istanbul, Turkey
    • Year and Date
      2008-08-13
    • Related Report
      2008 Final Research Report
  • [Presentation] Interactions between p27 and p88 replicase proteins of Red clover necrotic mosaic virus are required for viral RNA replication and RNA silencing suppression2008

    • Author(s)
      A. Mine, A. Takeda, M. Kaido, K. Mise and T. Okuno
    • Organizer
      XIV. International Congress of Virology
    • Place of Presentation
      Istanbul, Turkey
    • Year and Date
      2008-08-13
    • Related Report
      2008 Final Research Report
  • [Presentation] Host-specific roles of the 5' untranslated region (UTR) of Red clover necrotic mosaic virus RNA1 in cap-independent translation2008

    • Author(s)
      S. Sarawaneeyaruk, H. Murakami, H. Iwakawa, M. Kaido, K. Mise and T. Okuno
    • Organizer
      XIV. International Congress of Virology
    • Place of Presentation
      Istanbul, Turkey
    • Year and Date
      2008-08-13
    • Related Report
      2008 Final Research Report
  • [Presentation] ER-targeting of Red clover necrotic mosaic virus movement protein is dependent on the viral RNA replication2008

    • Author(s)
      M. Kaido, Y. Tsuno, K. Mise, T. Okuno
    • Organizer
      XIV. International Congress of Virology
    • Place of Presentation
      Istanbul. Turkey
    • Year and Date
      2008-08-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] A viral non-coding RNA generated by cis-element-mediated protection against 5'→3' decay represses both cap independent and dependent translation2008

    • Author(s)
      H. Iwakawa, H. Mizumoto, Y. Imoto, K. Takigawa, S. Sarawaneeyaruk, M. Kaido, K. Mise, T. Okuno
    • Organizer
      XIV. International Congress of Virology
    • Place of Presentation
      Istanbul. Turkey
    • Year and Date
      2008-08-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] Interactions between p27 and p88 replicase proteins of Red clover necrotic mosaic virus are required for viral RNA replication and RNA silencing suppression2008

    • Author(s)
      A. Mine, A. Takeda, M. Kaido, K. Mise, T. Okuno
    • Organizer
      XIV. International Congress of Virology
    • Place of Presentation
      Istanbul. Turkey
    • Year and Date
      2008-08-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] Host-specific roles of the 5'untranslated region (UTR) of Red clover necrotic mosaic virus RNA1 in cap-independent translation2008

    • Author(s)
      S. Sarawaneeyaruk, H. Murakami, H. Iwakawa, M. Kaido, K. Mise, T. Okuno
    • Organizer
      XIV. International Congress of Virology
    • Place of Presentation
      Istanbul. Turkey
    • Year and Date
      2008-08-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] Red clover necrotic mosaic virus RNAの複製は移行タンパク質の細胞内局在性に影響する2008

    • Author(s)
      築野靖子・三瀬和之・奥野哲郎・海道真典
    • Organizer
      平成20年度日本植物病理学会大会
    • Place of Presentation
      島根県松江市
    • Year and Date
      2008-04-27
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Red clover necrotic mosaic virus RNA1に由来するノンコーディングRNAの生成メカニズムと機能解析2008

    • Author(s)
      岩川弘宙・水本祐之・海道真典・三瀬和之・奥野哲郎
    • Organizer
      平成20年度日本植物病理学会大会
    • Place of Presentation
      島根県松江市析
    • Year and Date
      2008-04-27
    • Related Report
      2008 Final Research Report
  • [Presentation] Red clover necrotic mosaic virusの非構造タンパク質p27およびp88の相互作用はウイルス複製酵素複合体形成、RNA複製およびRNAサイレンシングの抑制に必要である2008

    • Author(s)
      峯彰・海道真典・三瀬和之・奥野哲郎
    • Organizer
      平成20年度日本植物病理学会大会
    • Place of Presentation
      島根県松江市
    • Year and Date
      2008-04-27
    • Related Report
      2008 Final Research Report
  • [Presentation] レッドクローバーネクロティックモザイクウイルスRNA2の3'UTRに存在する二つの新規なステムループ構造はRNA2の複製に重要である2008

    • Author(s)
      安夢楠・岩川弘宙・海道真典・三瀬和之・奥野哲郎
    • Organizer
      平成20年度日本植物病理学会大会
    • Place of Presentation
      島根県松江市
    • Year and Date
      2008-04-27
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] カーネーションリングスポットウイルスのゲノムRNA3'末端配列のウイルス感染性における意義2008

    • Author(s)
      永野秀昭・井本裕佳・海道真典・三瀬和之・奥野哲郎
    • Organizer
      平成20年度日本植物病理学会大会
    • Place of Presentation
      島根県松江市
    • Year and Date
      2008-04-27
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Red clover necrotic mosaic virus RNA1の3'翻訳促進因子によるキャップ非依存的翻訳はリボソームスキャニング機構を利用する2008

    • Author(s)
      サラワニヤラック シリラック・岩川弘宙・海道真典・三瀬和之・奥野哲郎
    • Organizer
      平成20年度日本植物病理学会大会
    • Place of Presentation
      島根県松江市
    • Year and Date
      2008-04-27
    • Related Report
      2008 Final Research Report
  • [Presentation] Melandrium yellow fleck virus (MYFV) RNA3の5'非翻訳領域の反復配列はNicotiana benthamianaでの全身感染に必須ではないがウイルスの感染競合に影響する2008

    • Author(s)
      楢林大樹・海道真典・奥野哲郎・三瀬和之
    • Organizer
      平成20年度日本植物病理学会大会
    • Place of Presentation
      島根県松江市
    • Year and Date
      2008-04-27
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Spring beauty latent virus (SBLV)感染シロイヌナズナに誘導される全身壊疽病徴発現への病害抵抗性経路の関与2008

    • Author(s)
      岩橋福松・林瑞恵・猿渡洋介・楢林大樹・海道真典・奥野哲郎・三瀬和之
    • Organizer
      平成20年度日本植物病理学会大会
    • Place of Presentation
      島根県松江市
    • Year and Date
      2008-04-27
    • Related Report
      2008 Final Research Report
  • [Presentation] Red clover necrotic mosaic virus RNA1に由来するノンコーディングRNAの生成メカニズムと機能解析2008

    • Author(s)
      岩川弘宙, 水本祐之, 海道真典, 三瀬和之, 奥野哲郎
    • Organizer
      平成20年度日本植物病理学会大会
    • Place of Presentation
      島根県松江市
    • Year and Date
      2008-04-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] Red clover necrotic mosaic virusの非構造タンパク質p27およびp88の相互作用はウイルス複製酵素複合体形成、RM複製およびRNAサイレンシングの抑制に必要である2008

    • Author(s)
      峯彰, 海道真典, 三瀬和之, 奥野哲郎
    • Organizer
      平成20年度日本植物病理学会大会
    • Place of Presentation
      島根県松江市
    • Year and Date
      2008-04-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] Red clover necrotic mosaic virus RNA1の3'翻訳促進因子によるキャップ非依存的翻訳はリボソームスキヤニング機構を利用する2008

    • Author(s)
      サラワニヤラックシリラック, 岩川弘宙, 海道真典, 三瀬和之, 奥野哲郎
    • Organizer
      平成20年度日本植物病理学会大会
    • Place of Presentation
      島根県松江市
    • Year and Date
      2008-04-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] Spring beauty latent virus (SBLV)感染シロイヌナズナに誘導される全身壊疸病徴発現への病害抵抗性経路の関与2008

    • Author(s)
      岩橋福松, 林瑞恵, 猿渡洋介, 楢林大樹, 海道真典, 奥野哲郎, 三瀬和之
    • Organizer
      平成20年度日本植物病理学会大会
    • Place of Presentation
      島根県松江市
    • Year and Date
      2008-04-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] Two TIR-NB-LRR genes, SSBIA and SSB1B, interdependently function to develop severe symptoms upon infection with Spring beauty latent virus in Arabidopsis thaliana2008

    • Author(s)
      Iwahashi, et. al.
    • Organizer
      Keystone Symposia on Molecular and Cellular Biology, Plant Innate Immunity
    • Place of Presentation
      Keystone Resort, Keystone, Colorado, USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] Brome mosaic virus(BMV)の3a移行タンパク質のリン酸化と管状構造2007

    • Author(s)
      赤松, ら
    • Organizer
      平成19年度日本植物病理学会関西部会
    • Place of Presentation
      岐阜市 岐阜大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] ブロムモザイクウイルスの移行タンパク質の機能解析-リン酸化と管状構造-2007

    • Author(s)
      赤松真子
    • Organizer
      第30回日本分子生物学会年会
    • Place of Presentation
      横浜市 パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Red clover necrotic mosaic virus RNA1の3'非翻訳領域に由来するSmall RNAの生成機構2007

    • Author(s)
      岩川弘宙
    • Organizer
      第55回日本ウイルス学会学術集会
    • Place of Presentation
      札幌市 札幌コンベンションセンター
    • Related Report
      2007 Annual Research Report
  • [Presentation] Red clover necrotic mosaic virusのRNA複製酵素成分間結合が複製とRNAサイレンシング抑制に与える影響2007

    • Author(s)
      峯 彰
    • Organizer
      第55回日本ウイルス学会学術集会
    • Place of Presentation
      札幌市 札幌コンベンションセンター
    • Related Report
      2007 Annual Research Report
  • [Presentation] Spring beauty latent virus (SBLV)感染シロイヌナズナに誘導される全身えそ病徴には2種のTIR-NB-LRR遺伝子が関与する2007

    • Author(s)
      猿渡洋介
    • Organizer
      平成19年度日本植物病理学会関西部会
    • Place of Presentation
      岐阜市 岐阜大学
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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