Analyses of insect mitochondrial DNA and its nuclear copies

• Project/Area Number: 19580058
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: MURAJI Masahiko National Institute of Agrobiological Sciences, 昆虫-昆虫・植物間相互作用研究ユニット, 上級研究員 (20355746)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 多型的DNAマーカー / 昆虫 / DNA塩基配列 / 偽遺伝子 / ミトコンドリアDNA / 核酸 / 分子進化

Research Abstract

Nuclear copies (Numts) of the mitochondrial DNA (mtDNA) are the major factor that deteriorate the utility of mtDNA markers in phylogenetic and population studies of insects. In this study, Numts were isolated and sequenced using various insect species. By comparing the sequences between Numts and mtDNA, the structure, evolutionary history, and utility of Numts in insect studies were examined.

Research Products

• [Journal Article] Relationship among local populations of the white grub beetle, Dasylepida ishigakiensis (Coleoptera: Scarabaeidae), detected by phylogenetic analysis based on the mitochondrial DNA sequence. (2010)
  • Author(s): Muraji M., Y. Hirai, T. Akino, S. Wakamura, H. Yasui, N. Arakaki, A. Nagayama, T. Ando, S. Tanizaki, Y. Hohama
  • Journal Title: Applied Entomology and Zoology (日本応用動物昆虫学会英文誌) 45巻(予定(印刷中)) Pages: 291-298
  • NAID: 130000248674
  • Peer Reviewed
• [Journal Article] PCR-RFLP analysis of Bactrocera dorsalis (Tephritidae; Diptera) complex species collected in and around the Ryukyu Islands of Japan using the mitochondrial A-T rich control region. (2010)
  • Author(s): Nakahara, S., M. Muraji
  • Journal Title: Research Bulletin of the Plant Protection Service, Japan (植物防疫所調査研究報告) 46巻 Pages: 17-23
  • NAID: 40017088620
  • Peer Reviewed
• [Journal Article] Relationship among local populations of the white grub beetle, Dasylepida ishigakiensis (Coleoptera : Scarabaeidae), detected by phylogenetic analysis based on the mitochondrial DNA sequence. (2009)
  • Author(s): M.Muraji, Y.Hirai, T.Akino, S.Wakamura, H.Yasui, N.Arakaki, A.Nagayama, T.Ando, S.Tanizaki, Y.Hohama
  • Journal Title: Applied Entomology and Zoology 45(2)(予定)(印刷中)
  • NAID: 130000248674
  • Peer Reviewed
• [Journal Article] PCR-RFLP analysis of Bactrocera dorsalis (Tephritidae ; Diptera) complex species Collected in and around the Ryukyu Islands of Japan Using the Mitochondrial A-T rich control region. (2009)
  • Author(s): S.Nakahara, M.Muraji
  • Journal Title: Research Bulletin of the Plant Protection Service Japan 46(印刷中)
  • NAID: 40017088620
  • Peer Reviewed
• [Journal Article] The Philippines is a possible source of the Bactrocera dorsalis complex species (Diptera, Tephritidae) occasionally flown into the Ryukyu Islands of Japan; analyses of the mitochondrial DNA. (2008)
  • Author(s): Muraji, M., S. Nakahara, T. Ishida, K. Minoura, I. Miyazaki, T. Kohama
  • Journal Title: Applied Entomology and Zoology (日本応用動物昆虫学会英文誌) 43巻 Pages: 609-615
  • Peer Reviewed
• [Journal Article] Phylogenetic analyses of Bactrocera fruit flies (Diptera: Tephritidae) based on nucleotide sequences of the mitochondrial DNA. (2008)
  • Author(s): Nakahara, S., M. Muraji
  • Journal Title: Research Bulletin of the Plant Protection Service, Japan(植物防疫所調査研究報告) 44巻
  • Peer Reviewed
• [Journal Article] Genetic variations among and within populations of the Oriental fruit fly, Bactrocera dorsalis (Diptera; Tephritidae), detected by PCR-RFLP of the mitochondrial control region. (2008)
  • Author(s): Nakahara, S., Y. Kobashigawa, M. Muraji
  • Journal Title: Applied Entomology and Zoology (日本応用動物昆虫学会英文誌) 43巻 Pages: 457-465
  • NAID: 110006878887
  • Peer Reviewed
• [Journal Article] Genetic variation of the green chafer, Anomala albopilosa (Hope) (Coleoptera: Scarabaeidae), in the Ryukyu Islands of Japan detected by mitochondrial DNA sequences. (2008)
  • Author(s): Muraji, M., N. Arakaki, S. Ohno, Y. Hirai
  • Journal Title: Applied Entomology and Zoology (日本応用動物昆虫学会英文誌) 43巻 Pages: 299-306
  • NAID: 110006684234
  • Peer Reviewed
• [Journal Article] Genetic divergence among populations of the white grub beetle, Dasylepida ishigakiensis (Coleoptera: Scarabaeidae), distributed in the southern part of the Ryukyu Islands of Japan, detected from the mitochondrial DNA sequences. (2008)
  • Author(s): Muraji, M., Y. Hirai, T. Akino, S. Wakamura, N. Arakak
  • Journal Title: Applied Entomology and Zoology (日本応用動物昆虫学会英文誌) 43巻 Pages: 287-292
  • NAID: 110006684232
  • Peer Reviewed
• [Journal Article] 南西諸島と周辺域におけるアオドウガネの遺伝的変異と地理的分布. (2008)
  • Author(s): 村路雅彦・新垣則雄・大野豪・平井剛夫
  • Journal Title: Saikaku Tsushin 17巻 Pages: 51-57
• [Journal Article] The Philippines is a possible source of the Bactrocera dorsalis complex species (Diptera, Tephritidae) occasionally flown into the Ryukyu Islands of Japan ; analyses of the mitochondrial DNA (2008)
  • Author(s): Muraji, M., S. Nakahara, T. Ishida, K. Minoura, I. Miyazaki, T. Kohama
  • Journal Title: Applied Entomology and Zoology 43 Pages: 609-615
  • Peer Reviewed
• [Journal Article] Genetic variations among and within populations of the Oriental fruit fly, Bactrocera dorsalis (Diptera ; Tephritidae), detected by PCR-RFLP of the mitochondrial control region (2008)
  • Author(s): Nakahara, S., Y. Kobashigawa, M. Muraji
  • Journal Title: Applied Entomology and Zoology 43 Pages: 457-465
  • NAID: 110006878887
  • Peer Reviewed
• [Journal Article] Phylogenetic analyses of Bactrocera fruit flies (Diptera : Tephritidae) based on nucleotide sequences of the mitochondrial DNA (2008)
  • Author(s): Nakahara, S., M. Muraji
  • Journal Title: Research Bulletin of the Plant Protection Service Japan 44 Pages: 1-12
  • Peer Reviewed
• [Journal Article] 南西諸島と周辺域におけるアオドウガネの遺伝的変異と地理的分布 (2008)
  • Author(s): 村路雅彦, 新垣則雄, 大野豪, 平井剛夫
  • Journal Title: SAIKAKU TSOSHIN (鰓角通信) 17 Pages: 51-57
• [Journal Article] Genetic divergence among populations of the white grub beetle,Dasylepida ishigakiensis(Coleoptera:Scarabaeidae),distributed in the southern part of the Ryukyu Islands of Japan,detected from mitochondrial DNA sequences (2008)
  • Author(s): Masahiko Muraji
  • Journal Title: Applied Entomology and Zoology(日本応用動物昆虫学会英文誌) 43 Pages: 287-292
  • Peer Reviewed
• [Journal Article] Genetic variation of the green chafer,Anomala albopilosa(Hope)(Coleoptera:Scarabaeidae),in the Ryukyu Islands of Japan detected by mitochondrial DNA sequences. (2008)
  • Author(s): Masahiko Muraji
  • Journal Title: Applied Entomology and Zoology(日本応用動物昆虫学会英文誌) 43 Pages: 299-306
  • Peer Reviewed
• [Presentation] ネムリユスリカの系統地理学的研究:ナイジェリアとマラウィ個体群間の比較 (2009)
  • Author(s): GusevOleg, 小林貞, 中原雄一, 岩田健一, Cornette Richard, 金森保志, 黄川田隆洋, 村路雅彦, 奥田隆
  • Organizer: 第53回 日本応用動物昆虫学会大会
  • Place of Presentation: 北海道大学高等教育機能開発センター
  • Year and Date: 2009-03-29
• [Presentation] 沖縄県に飛来するミカンコミバエ種群の遺伝的変異フィリピンからの直接飛来の可能性について (2008)
  • Author(s): 中原重仁, 宮崎勲, 小濱継雄, 村路雅彦
  • Organizer: 第52回日本応用動物昆虫学会、第52回日本応用動物昆虫学会大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2008-03-28
• [Presentation] 先島諸島におけるケブカアカチャコガネの遺伝的変異 (2008)
  • Author(s): 村路雅彦, 平井剛夫, 秋野順治, 若村定男, 新垣則雄
  • Organizer: 第52回日本応用動物昆虫学会大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2008-03-28
• [Presentation] 東日本におけるプラタナスグンバイの遺伝的変異(予報) (2008)
  • Author(s): 村路雅彦, 立石剣
  • Organizer: 第52回日本応用動物昆虫学会大会、第52回日本応用動物昆虫学会大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2008-03-27
• [Presentation] 東日本におけるプラタナスグンバイの遺伝的変異(予報) (2008)
  • Author(s): 村路 雅彦
  • Organizer: 第52回日本応用動物昆虫学会大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2008-03-27
• [Presentation] 沖縄県に飛来するミカンコミバエ種群の遺伝的変異フィりピンからの直接飛来の可能性 (2008)
  • Author(s): 中原 重仁
  • Organizer: 第52回日本応用動物昆虫学会大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2008-03-27