Utilization of organic phosphate by acid phosphatase released from external hyphae of mycorrhizal fungi

• Project/Area Number: 19580064
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Yamagata University
• Principal Investigator: TAWARAYA Keitaro Yamagata University, 農学部, 教授 (70179919)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: アーバスキュラー菌根菌 / リン酸 / 菌糸の浸出物 / ボスファターゼ / 菌根 / リン / ホスファターゼ / 菌糸 / 浸出物

Research Abstract

各種土壌からアーバスキュラー菌根菌を単離し、コンパートメントポット培養により菌糸の浸出物を含む土壌溶液を回収し、酸性ホスファターゼ活性を測定したところ、泥炭土壌から単離したGlomus clarumが高い酸性ホスファターゼ活性を示した。この酸性ホスファターゼ活性はフッ化ナトリウム及びモリブデン酸ナトリウムにより阻害された。SDS-PAGEにより菌糸の浸出物中の酸性ホスファターゼは菌特異的であることが明らかとなった。

Research Products

• [Journal Article] Improvement of early growth of two tropical peat-swamp forest tree species Ploiarium alternifolium and Calophyllum hosei by two arbuscular mycorrhizal fungi under greenhouse conditions (2008)
  • Author(s): Turjaman M, Tamai Y, Sitepu IR, Santoso E, Osaki M and Tawaraya K
  • Journal Title: New Forests 36 Pages: 1-12
  • Peer Reviewed
• [Journal Article] Improvement of early growth of two tropical peat-swamp forest tree species Ploiarium alternifolium and Calophyllum hosei by two arbuscular mycorrhizal fungi under greenhouse conditions (2008)
  • Author(s): Turjaman, M., Tamai, Y., Tawaraya, K.
  • Journal Title: New Forests 36 Pages: 1-12
  • Peer Reviewed
• [Journal Article] Effect of arbuscular mycorrhizal colonization on nitrogen and phosphorus uptake and growth of Aloe vera l (2007)
  • Author(s): Tawaraya K, TurJaman M and Ekamawanti HA
  • Journal Title: Hortscience 42 Pages: 1737-1739
  • Peer Reviewed
• [Journal Article] Formation of appressoria by the arbuscular mycorrhizal fungus Gigaspora margarita on roots of Allium cepa is linked with root age (2007)
  • Author(s): Tawaraya K, Watanabe S, Vierheilig H and Wagatsuma T
  • Journal Title: Mycoscience 48 Pages: 305-308
  • NAID: 10019950677
  • Peer Reviewed
• [Journal Article] White clover living mulch increases the yield of silage corn via arbuscular mycorrhizal fungus colonization (2007)
  • Author(s): Deguchi S, Shimazaki Y, Uozumi S, Tawaraya K, Kawamoto H and Tanaka O
  • Journal Title: Plant Soil 291 Pages: 291-299
  • Peer Reviewed
• [Journal Article] Effect of arbuscular mycorrhizal colonization on nitrogen and phosphorus uptake and growth of Aloe vera L. (2007)
  • Author(s): Tawaraya, K.
  • Journal Title: HortScience 42 Pages: 1737-1739
  • Peer Reviewed
• [Journal Article] Formation of appressorium by the arbuscular mycorrhizal fungus Gigas pora margarita on roots of Allium cepa is linked with root age (2007)
  • Author(s): Tawaraya, K.
  • Journal Title: Mycoscience 48 Pages: 305-308
  • Peer Reviewed
• [Presentation] 圃場におけるネギ生育に対するアーバスキュラー菌根菌の影響 (2008)
  • Author(s): 廣瀬僚太、我妻忠雄、俵谷圭太郎
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-09-09
• [Presentation] Reduction of phosphorus fertilization to Allium fistulosum with inoculation of arbuscular mycorrhizal fungi (2007)
  • Author(s): Hirose, R.
  • Organizer: Eighth Conference of the East and Southeast Asian Federatin of Soil Science
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2007-10-22
• [Presentation] アーバスキュラー菌根菌を利用したネギ品種におけるリン酸施肥削減 (2007)
  • Author(s): 廣瀬僚太
  • Organizer: 日本土壌肥料学会東京大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2007-08-24
• [Presentation] アーバスキュラー菌根菌を利用したネギ品種におけるリン酸施肥削減 (2007)
  • Author(s): 廣瀬僚太、元鍾大、我妻忠雄、俵谷圭太郎
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 東京
  • Year and Date: 2007-08-22