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Study of environmental adaptation and horizontal gene transfer of soybean-nodulating rhizobia

Research Project

Project/Area Number 19580069
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionUniversity of Miyazaki

Principal Investigator

SAEKI Yuichi  University of Miyazaki, 農学部, 准教授 (50295200)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsダイズ / Rj遺伝子型 / 根粒菌 / 遺伝子水平伝播 / 環境適応性 / ダイズ根粒菌 / Bradyrhizobium / 環境傾度 / 土壌pH / 土壌温度 / Rj遺伝子 / Sinorhizobium
Research Abstract

In this study, we have studied to clarify the mechanism of occupancy of soybean-nodulating rhizobia under various environmental conditions. We clarified environmental factors, soil temperature and soil pH for establishment of soybean-nodulating rhizobia flora through the analyses about bacterial culture and composition of flora of soybean-nodulating rhizobia under various temperature, gene expression analysis under various pH conditions, and horizontal gene transfer.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (39 results)

All 2010 2009 2008 2007 Other

All Journal Article (15 results) (of which Peer Reviewed: 13 results) Presentation (21 results) Remarks (3 results)

  • [Journal Article] ダイズ根粒菌の外来遺伝子獲得に関する菌株間差異の解析.2010

    • Author(s)
      城惣吉, 山本昭洋, 吉田ナオト, 佐伯雄一
    • Journal Title

      宮崎大学農学部研究報告 55巻

      Pages: 79-87

    • NAID

      110008658917

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] ダイズ根粒菌の外来遺伝子獲得に関する菌株間差異の解析2010

    • Author(s)
      城惣吉, 山本昭洋, 吉田ナオト, 佐伯雄一
    • Journal Title

      宮崎大学農学部研究報告 56

      Pages: 79-87

    • NAID

      110008658917

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intragenomic variation of internal transcribed spacer regions between 16S-23S rRNA genes among the three copies of Sinorhizobium fredii strains.2009

    • Author(s)
      Saeki Y, Oguro H, Akagi I, Yamakawa T, Yamamoto A
    • Journal Title

      Soil Science and Plant Nutrition 55

      Pages: 627-633

    • NAID

      110007502755

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 日本国内におけるダイズ根粒菌の分布と多様性~共生窒素固定の農業技術としての有効利用を目指して~2009

    • Author(s)
      佐伯雄一
    • Journal Title

      化学と生物 47巻

      Pages: 228-230

    • NAID

      10026435129

    • Related Report
      2009 Final Research Report
  • [Journal Article] Estimation of nodulation tendency among Rj-genotype soybeans by bradyrhizobial community isolated from an Andosol.2009

    • Author(s)
      Minami M, Yamakawa T, Yamamoto A, Akao S, Saeki Y
    • Journal Title

      Soil Science and Plant Nutrition 54

      Pages: 65-72

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intragenomic variation of internal transcribed spacer regions between 16S-23S rRNA genes among the three copies of Sinorhizobium fredii strains2009

    • Author(s)
      Saeki Y, Oguro H, Akagi I, Yamakawa T, Yamamoto A
    • Journal Title

      Soil Science and Plant Nutrition 55

      Pages: 627-633

    • NAID

      110007502755

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 日本国内におけるダイズ根粒菌の分布と多様性~共生窒素固定の農業技術としての有効利用を目指して~2009

    • Author(s)
      佐伯雄一
    • Journal Title

      化学と生物 47

      Pages: 228-230

    • NAID

      10026435129

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Estimation of nodulation tendency among Rj-genotype soybeans by bradyrhizobial community isolated from an Andosol2009

    • Author(s)
      Minami M, et al.
    • Journal Title

      Soil Science and Plant Nutrition 54

      Pages: 65-72

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Estimation of bacterial community diversity of soybean-nodulating bradyrhizobia isolated from Rj-genotype soybeans.2008

    • Author(s)
      Saeki Y, Minami M, Yamamoto A, Akao S
    • Journal Title

      Soil Science and Plant Nutrition 54

      Pages: 718-724

    • NAID

      110007331015

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diversity and Distribution of Indigenous Soybean-Nodulating Rhizobia in the Okinawa Islands2008

    • Author(s)
      Suzuki K, Oguro H, Yamakawa T, Yamamoto A, Akao S, Saeki Y
    • Journal Title

      Japan. Soil Science and Plant Nutrition 54

      Pages: 237-246

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diversity and Distribution of Indigenous Soybean-Nodulating Rhizobia in the Okinawa Islands, Japan2008

    • Author(s)
      Suzuki K, et al.
    • Journal Title

      Soil Science and Plant Nutrition 54

      Pages: 237-246

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Estimation of bacterial community diversity of soybean-nodulating bradyrhizobia isolated from Rj-genotype soybeans2008

    • Author(s)
      Saeki Y, et al.
    • Journal Title

      Soil Science and Plant Nutrition 54

      Pages: 718-724

    • NAID

      110007331015

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diversity and Distribution of Indigenous Soybean-Nodulating Rhizobia in the Okinawa Islands, Japan2008

    • Author(s)
      Suzuki K, et. al.
    • Journal Title

      Soil Science and Plant Nutrition 54(In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differentiation of soybean-nodulating Bradyrhziobium USDA strains based on restriction fragment length polymorphisms of 23S-5S rRNA genes.2007

    • Author(s)
      Saeki Y, Murata T, Yamakawa T, Akao S
    • Journal Title

      Soil Science and Plant Nutrition 53

      Pages: 562-567

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differentiation of soybean-nodulating Bradyrhziobium USDA strains based on restriction fragment length polymorphisms of 23S-5S rRNA genes2007

    • Author(s)
      Saeki Y, et. al.
    • Journal Title

      Soil Science and Plant Nutrition 53

      Pages: 562-567

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] ダイズの根粒着生におけるRj遺伝子型と温度の影響2009

    • Author(s)
      城惣吉, 南麻衣, 梅原洋佐, 林正紀, 山本昭洋, 佐伯雄一
    • Organizer
      日本土壌肥料学会2009年度京都大会
    • Place of Presentation
      京都
    • Related Report
      2009 Final Research Report
  • [Presentation] Rj遺伝子型ダイズの根粒菌選択性に関する群集構造解析2009

    • Author(s)
      田島稔之, 大塚亜沙子, 梅原洋佐, 林正紀, 山本昭洋, 佐伯雄一
    • Organizer
      日本土壌肥料学2009年度京都大会
    • Place of Presentation
      京都
    • Related Report
      2009 Final Research Report
  • [Presentation] ダイズ根粒菌Bradyrhizobium japonicumのアルカリ耐性とnha遺伝子発現2009

    • Author(s)
      小黒紘子, 山本昭洋, 佐伯雄一
    • Organizer
      日本土壌微生物学会2009年度大会
    • Place of Presentation
      福岡
    • Related Report
      2009 Final Research Report
  • [Presentation] ダイズ根粒菌の外来遺伝子獲得機構に関する検討2009

    • Author(s)
      城惣吉, 山本昭洋, 佐伯雄一
    • Organizer
      日本土壌肥料学会九州支部春季例会
    • Place of Presentation
      佐賀
    • Related Report
      2009 Final Research Report
  • [Presentation] ダイズの根粒着生におけるRj遺伝子型と温度の影響2009

    • Author(s)
      城惣吉, 南麻衣, 梅原洋佐, 林正紀, 山本昭洋, 佐伯雄一
    • Organizer
      日本土壌肥料学会2009年度京都大会
    • Place of Presentation
      京都大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] Rj遺伝子型ダイズの根粒菌選択性に関する群集構造解析2009

    • Author(s)
      田島稔之, 大塚亜沙子, 梅原洋佐, 林正紀, 山本昭洋, 佐伯雄一
    • Organizer
      日本土壌肥料学会2009年度京都大会
    • Place of Presentation
      京都大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] ダイズ根粒菌 Bradyrhiaobium japonicum のアルカリ耐性とnha遺伝子発現2009

    • Author(s)
      小黒紘子, 山本昭洋, 佐伯雄一
    • Organizer
      日本土壌微生物学会2009年度大会
    • Place of Presentation
      九州大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] ダイズ根粒菌の外来遺伝子獲得機構に関する検討2009

    • Author(s)
      城惣吉, 山本昭洋, 佐伯雄一
    • Organizer
      日本土壌肥料学会九州支部春季例会
    • Place of Presentation
      佐賀大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] 特定根粒菌株に対して非親和性を示すRj4遺伝子保有ダイスの解析2008

    • Author(s)
      林 正紀ら
    • Organizer
      日本育種学会
    • Place of Presentation
      滋賀県立大学 (滋賀)
    • Year and Date
      2008-10-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] 土着ダイス根粒菌の占有率に及ぼす温度の影響2008

    • Author(s)
      小住哲ら
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋市立大学 (愛知)
    • Year and Date
      2008-09-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] 日本の土着ダイス根粒菌の群集構造と分布に関する研究2008

    • Author(s)
      田島稔之ら
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋市立大学 (愛知)
    • Year and Date
      2008-09-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] ダイス根粒菌の分布と多様性に関する分子生態学的研究2008

    • Author(s)
      佐伯雄一
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋市立大学 (愛知)
    • Year and Date
      2008-09-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] 梅原洋佐特定根粒菌株に対して非親和性を示すRj4遺伝子保有ダイズの解析2008

    • Author(s)
      林正紀, 佐伯雄一, 西岡美樹, 高橋将一, 佐山貴司, 石本政男, 原田久也, 河内宏
    • Organizer
      日本育種学会第114回大会
    • Place of Presentation
      滋賀
    • Related Report
      2009 Final Research Report
  • [Presentation] ダイズ根粒菌の分布と多様性に関する分子生態学的研究2008

    • Author(s)
      佐伯雄一
    • Organizer
      日本土壌肥料学会2008年度愛知大会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Final Research Report
  • [Presentation] 土着ダイズ根粒菌の占有率に及ぼす温度の影響2008

    • Author(s)
      小住哲, 小黒紘子, 山本昭洋, 佐伯雄一
    • Organizer
      日本土壌肥料学会2008年度愛知大会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Final Research Report
  • [Presentation] 日本の土着ダイズ根粒菌の群集構造と分布に関する研究2008

    • Author(s)
      田島稔之, 南麻衣, 山本昭洋, 佐伯雄一
    • Organizer
      日本土壌肥料学会2008年度愛知大会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Final Research Report
  • [Presentation] ダイズ根粒菌の宿主への感染傾向に関する解析 -根粒着生・培養を通して見える根粒菌生態-2007

    • Author(s)
      佐伯 雄一
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京農業大学(東京)
    • Year and Date
      2007-08-24
    • Related Report
      2007 Annual Research Report
  • [Presentation] ダイズ根粒菌の宿主への感染傾向に関する解析-根粒着生・培養を通して見える根粒菌生態-2007

    • Author(s)
      佐伯雄一
    • Organizer
      日本土壌肥料学会2007年度東京大会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] 宿主遺伝子型による土着ダイズ根粒菌の分離傾向2007

    • Author(s)
      南麻衣, 山本昭洋, 佐伯雄一, 赤尾勝一郎
    • Organizer
      日本土壌肥料学会2007年度東京大会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] ダイズ根粒菌Sinorhizobium属USDA株の16S-23S rDNAITS領域の多様性2007

    • Author(s)
      小黒紘子, 佐伯雄一, 山本昭洋, 赤尾勝一郎
    • Organizer
      日本土壌肥料学会2007年度東京大会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] 赤尾勝一郎ダイズ根粒菌の土壌中における占有率評価法の開発2007

    • Author(s)
      小住哲, 堀真治, 佐伯雄一, 山本昭洋
    • Organizer
      日本土壌肥料学会九州支部春期例会
    • Place of Presentation
      福岡
    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.agr.miyazaki-u.ac.jp/~abs/sspn/index.html

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.agr.miyazaki-u.ac.jp/~abs/sspn/index.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.agr.miyazaki-u.ac.jp/~abs/index.html

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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