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Requirement of PKC pathway of target yeast for toxicity of killer protein

Research Project

Project/Area Number 19580100
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionNational Institute for Agro-Environmental Sciences

Principal Investigator

KITAMOTO Hiroko  National Institute for Agro-Environmental Sciences, 生物生態機能研究領域, 主任研究員 (10370652)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords微生物機能 / 細胞内・細胞間情報伝達 / 膜輸送と輸送蛋白質 / 応用微生物 / シグナル伝達 / 抗生物質 / キチン合成酵素 / PKC経路
Research Abstract

Toxic subunit of Kluyveromyces lactis killer protein (zymocin), is transported into cell of sensitive yeast, Saccharomyces cerevisiae (SC). In this study we found that the activation of cell intregrity pathway (PKC pathway) of SC is needed for the transportation.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report

Research Products

(24 results)

All 2010 2009 2008 2007

All Journal Article (8 results) (of which Peer Reviewed: 6 results) Presentation (14 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Mannosylinositol phosphorylceramide is a major sphingolipid component and is required for proper localization of plasma membrane proteins in Schizosaccharomyces pombe2010

    • Author(s)
      Nakase M., M. Tani, T. Morita, H.K. Kitamoto, J. Kashiwazaki, T. Nakamura, A. Hosomi, N. Tanaka, K. Takegawa
    • Journal Title

      Journal of Cell Science 123

      Pages: 1578-1587

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cellular and transcriptional responses of yeast to the cleavage of cytosolic tRNAs induced by colicin D.2009

    • Author(s)
      Megumi S., O. Tetsuhiro, K. Atsuhiko, H.K. Kitamoto, H. Makoto, H. Masaki
    • Journal Title

      Yeast 26(12)

      Pages: 663-673

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 真菌界の殺し屋-酵母のキラー因子生物の科学遺伝2009

    • Author(s)
      北本宏子
    • Journal Title

      63,2

      Pages: 48-53

    • Related Report
      2009 Final Research Report
  • [Journal Article] Cellular and transcriptional responses of yeast to the cleavage of cyto solic tRNAs induced by colicin D2009

    • Author(s)
      Megumi Shigematsu, Tetsuhiro Ogawa, Atsuhiko Kido, Hiroko Kitamoto, Makoto Hidaka, Haruhiko Masaki
    • Journal Title

      YEAST 26

      Pages: 663-673

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 真菌界の殺し屋-酵母のキラー因子2009

    • Author(s)
      北本宏子
    • Journal Title

      生物の科学 遺伝 63

      Pages: 48-53

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Rice exonuclease-1 homologue, OsEXO1, that interacts with DNA polymerase □ and RPA subunit proteins, is involved in cell proliferation2008

    • Author(s)
      Furukawa T, et al.
    • Journal Title

      Plant Mol Biol 66

      Pages: 519-531

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two Alternatively Spliced Transcripts Generated from OsMUS81,a Rice Homolog of Yeast MUS81, Are Up-Regulated by D NA-Damaging Treatments2007

    • Author(s)
      Naozumi Mimida, et. al.
    • Journal Title

      PLANT AND CELL PHYSIOLOGY 48

      Pages: 648-654

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rice Shaker Postassium Channel OsKAT1 Confers Tolerance to Salinity Stress on Yeast and Rice Cells12007

    • Author(s)
      Toshihiro Obata, et. al.
    • Journal Title

      PLANT PHYSIOLOGY 144

      Pages: 1978-1985

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 自然環境下で抗菌タンパク質耐性株は定着するのか?2009

    • Author(s)
      柴山祥枝, 多胡香奈子, 中村幸治, 北本宏子
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2009-07-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 自然環境下で抗菌タンパク質耐性株は定着するのか?2009

    • Author(s)
      柴山祥枝, 多胡香奈子, 中村幸治, 北本宏子
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      茨城県
    • Related Report
      2009 Final Research Report
  • [Presentation] tRNAの切断が接合シグナル伝達系を活性化させる2009

    • Author(s)
      茂松恵, 大本哲也, 小川哲弘, 北本宏子, 日高真誠, 正木春彦
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      茨城県
    • Related Report
      2009 Final Research Report
  • [Presentation] Cellularand transcriptional responses to tRNA cleavage induced by tRNA-targeting ribonuclease2009

    • Author(s)
      M. Shigematsu, T. Omoto, T. Ogawa, H.K. Kitamoto, M. Hidaka, H. Masaki
    • Organizer
      colicin D., Yeast cell biology meeting
    • Place of Presentation
      米国
    • Related Report
      2009 Final Research Report
  • [Presentation] tRNA切断とDNA損傷修復機構との関連性の検討2009

    • Author(s)
      茂松恵, ら
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      マリンメッセ福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] Kluyveromyces lactisアルコール脱水素酵素Iにおけるニッケル結合部位の推定2008

    • Author(s)
      前田美紀, 北本宏子, 梶原英之
    • Organizer
      第8回日本蛋白質科学会年会日本蛋白質科学会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] 出芽酵母の偽菌糸形成における新しい形態変化2008

    • Author(s)
      柴山祥枝, 中村幸治, 北本宏子
    • Organizer
      日本農芸化学会大会講演要旨集2008年度(平成20年度)大会, (社)日本農芸化学会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] tRNAノックダウンに対するDNA損傷応答の関与2008

    • Author(s)
      重松恵, ら
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      北海道大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] カラシナ由来ディフェンシンの抗菌作用に関与する遺伝子の検索2008

    • Author(s)
      多胡 香奈子, ら
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      名城大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] 出芽酵母におけるカラシナ由来ディフェンシンの抗菌作用に関与する遺伝子の特定2007

    • Author(s)
      多胡香奈子, 高久洋暁, 高木正道, 北本宏子
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      大阪
    • Related Report
      2009 Final Research Report
  • [Presentation] 出芽酵母におけるカラシナ由来ディフェンシンの抗菌作用に関与する遺伝子の特定2007

    • Author(s)
      多胡 香奈子, ら
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      大阪大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] tRNA切断性トキシンに対する出芽酵母の細胞応答の解析2007

    • Author(s)
      茂松 恵, ら
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] 分裂酵母のスフィンゴ脂質の生合成機構と役割について2007

    • Author(s)
      中瀬 舞, ら
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      大阪大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] tRNA切断性トキシンに対する出芽酵母の細胞応答の解析2007

    • Author(s)
      茂松 恵, ら
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      大阪大学
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] カルシニューリン活性化剤2010

    • Inventor(s)
      北本宏子, 宮川都吉
    • Industrial Property Rights Holder
      農業生物資源研究所
    • Acquisition Date
      2010-04-30
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] キラー蛋白質の精製方法2010

    • Inventor(s)
      北本宏子
    • Industrial Property Rights Holder
      農業生物資源研究所
    • Acquisition Date
      2010-04-16
    • Related Report
      2009 Final Research Report

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Published: 2007-03-31   Modified: 2016-04-21  

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