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Studies on Functional Analysis and Regulation Mechanism of Plant Aquaporins

Research Project

Project/Area Number 19580106
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionShimane University

Principal Investigator

SHIBATA Hitoshi  Shimane University, 生物資源科学部, 教授 (40032601)

Co-Investigator(Kenkyū-buntansha) KATSUHARA Maki  岡山大学, 資源生物学研究所, 准教授 (00211847)
石川 孝博  島根大学, 生物資源科学部, 准教授 (60285385)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords水チャンネル / アクアポリン / タンパク質リン酸化 / 脱リン酸化 / 花弁開閉 / 温度依存的応答 / 温度依存的植物応答 / タンパク質脱リン酸化
Research Abstract

チューリップ花弁の開閉は温度依存的であり、開花のためには水の花弁への輸送が必須で、水チャンネルの活性化が起こる。水チャネルを構成するアクアポリンの分子種を同定するために、細胞質アクアポリン遺伝子4種類をクローニングし、TgPIP2:2が水の輸送に関与することを機能面から確証した。またアクアポリンがリン酸化されて活性型になり、脱リン酸化で不活性化されることを確認した。TgPIP2:2も分子内でリン酸化を受ける3種のセリン残基(Ser35, Ser116, Ser274)を特定した。また、PIP2:2が花弁、茎、葉、塊茎、根いずれの組織においても最も発現量が多い分子種であり、根から花弁にいたる水の輸送に関与している可能性を指摘した。
TgPIP2-2を酵母(ピキア)に発現させ、水チャンネル活性を図る手法を新たに導入した。リン酸化脱リン酸化の阻害剤が活性に影響したことから、簡便に水チャンネル活性を測定できる系であるとともに調節機構を検証するための優れた方法であると結論した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report

Research Products

(12 results)

All 2009 2008 2007 Other

All Journal Article (8 results) (of which Peer Reviewed: 4 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Heterologouse Expression of Tulip Petal Plasma Membrane Aquaporinin Pichia pastoris for Water Channel2009

    • Author(s)
      Abul Kalam Azad, Yoshihiro Sawa,Takahiro Ishikawa and Hitoshi Shibata
    • Journal Title

      Applied and Environmental Microbiology 75

      Pages: 2792-2797

    • Related Report
      2008 Final Research Report
  • [Journal Article] Heterologouse Expression of Tulip Petal Plasma Membrane Aquaporins in Pichia pastoris for Water Channel Analysis2009

    • Author(s)
      A. K. Azad, Y. Sawa, T. Ishikawa, H. Shibata
    • Journal Title

      Applied and Environmental Microbiology 75(印刷中)(Web公開中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intaracellular energy depletion triggers programmed cell death during petal senescence in tulip2008

    • Author(s)
      Abul Kalam Azad, Takayuki Ishikawa, Takahiro Ishikawa, Yoshihiro Sawa, and Hitoshi Shibata
    • Journal Title

      Journal of Experimental Botany 59

      Pages: 2085-2095

    • Related Report
      2008 Final Research Report
  • [Journal Article] Characterization of Four Plasma Membrane Aquaporins in Tulip Petals : A Putative Homolog is Regulated by Phosphorylation2008

    • Author(s)
      Abul Kalam Azad, Maki Katsuhara,Yoshihiro Sawa, Takahiro Ishikawa and Hitoshi Shibata
    • Journal Title

      Plant and Cell Physiology 49

      Pages: 1196-1208

    • NAID

      10027332956

    • Related Report
      2008 Final Research Report
  • [Journal Article] Intracelluar energy depletion triggers programmed celldeath during petal senescence in tulip2008

    • Author(s)
      A. K. Azad, T. Ishikawa, T. Ishikawa, Y. Sawa, H. Shibata
    • Journal Title

      Journal of Experimental Botany 59

      Pages: 2085-2095

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Four Plasma Membrane Aquaporins in Tulip Petals ; A putative Homolog is Regulated by Phosphorylation2008

    • Author(s)
      A. K. Azad, M. Katsuhara, Y. Sawa, T. Ishikawa H. Shibata
    • Journal Title

      Plant & Cell Physiology 49

      Pages: 1196-1208

    • NAID

      10027332956

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temperature-dependent stomatal movement in tulip petal controls water transpiration during flower opening and closing2007

    • Author(s)
      Abul Kalam Azad, Yoshihiro Sawa, Takahiro Ishikawa and Hitoshi Shibata
    • Journal Title

      Annals of Applied Biology 150

      Pages: 81-87

    • Related Report
      2008 Final Research Report
  • [Journal Article] Temperature-dependent stomatal movement in tulip petals controls water transpiration during flower opening and closing2007

    • Author(s)
      A. K. Azad, Y. Sawa, T. Ishikawa, And H. Shibata
    • Journal Title

      Annals of Applied Biology 150

      Pages: 81-87

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Xenopasu oocytesとPichia pastorisで発現させたチューリップ花弁由来アクアポリンの水チャンネル活性:ホモログTgPIP2;2はリン酸化によって制御される2008

    • Author(s)
      Abul Kalam Azad, Maki Katsuhara,Yoshihiro Sawa,Takahiro Ishikawa and Hitoshi Shibata
    • Organizer
      第49回日本植物生理学会年会要旨集、pp.305
    • Place of Presentation
      札幌
    • Related Report
      2008 Final Research Report
  • [Presentation] Molecular Cloning,Functional and Expression Analysis of Four Homologues of Plasama Membrane Aquaporin Subfamilies of the Tulip Petal Tulipa Gesneriana2007

    • Author(s)
      Abul Kalam Azad, Maki Katsuhara,Yoshihiro Sawa,Takahiro Ishikawa and Hitoshi Shibata
    • Organizer
      The 5^<th> International Conference of Aquaporin. Exploring new functions of Aquaporin. Pp.93
    • Place of Presentation
      Nara, Japan
    • Related Report
      2008 Final Research Report
  • [Presentation] Molecular cloning, functional and expression analysis of four Homologues of plasma membrane aquaporin subfamilies of the tulip Petal Tulipa gesneriana2007

    • Author(s)
      A. K. Azad, M. Ktsuhara, Y. Sawa, T. Ishikawa and H. Shibata
    • Organizer
      The 5th International Congress of Aquaporin
    • Place of Presentation
      奈良Centennial Hall
    • Related Report
      2007 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2008 Final Research Report

URL: 

Published: 2007-03-31   Modified: 2016-04-21  

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