Elucidation of novel roles of F-box protein that recognizes sugar chain.

• Project/Area Number: 19580114
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Tokyo Metropolitan Organization for Medical Research
• Principal Investigator: YOSHIDA Yukiko Tokyo Metropolitan Organization for Medical Research, 東京都臨床医学総合研究所, 主任研究員 (90271543)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: F-box蛋白質 / ユビキチンリガーゼ / N型糖鎖 / 標的分子

Research Abstract

私達が糖鎖を認識するF-box蛋白質として発見したFbs1はSCF型ユビキチンリガーゼの基質認識サブユニットとして糖蛋白質をユビキチン化し、細胞における品質管理機構である小胞体関連分解に関わるものとして機能することを報告してきた。本研究では、Fbs1がユビキチンリガーゼとして機能するばかりではなく、血管新生に関わる蛋白質Amotと複合体を形成していることを見出し、その機能について解析を行った。

Research Products

• [Journal Article] 蛋白質の一生 (2008)
  • Author(s): 吉田雪子
  • Journal Title: 蛋白質核酸酵素臨時増刊 53巻 Pages: 1050-1050
• [Journal Article] SCF^<Fbs1>の構造と機能 (2008)
  • Author(s): 水島恒裕、吉田雪子
  • Journal Title: 実験医学臨時増刊「細胞内の輪廻転生.タンパク質の分解機構 26巻 Pages: 81-86
• [Journal Article] SCF^<Fbs1>の構造と機能 (2008)
  • Author(s): 水島恒裕, 吉田雪子
  • Journal Title: 実験医学臨時増刊「細胞内の輪廻転生.タンパク質の分解機構」 26 Pages: 81-86
• [Journal Article] Fbs1 (2008)
  • Author(s): 吉田雪子
  • Journal Title: キーワード:蛋白質の一生.蛋白質核酸酵素臨時増刊 53 Pages: 1050-1050
• [Journal Article] F-box proteins that contain sugar-binding domains. (2007)
  • Author(s): Y. Yoshida
  • Journal Title: Biosci. Biotechnol. Biochem. 71 Pages: 2623-2631
  • NAID: 10027520502
  • Peer Reviewed
• [Journal Article] Fbs1 protects the malfolded glycoproteins from the attack of peptide:N-glycanase. (2007)
  • Author(s): Y. Yamaguchi et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 362 Pages: 712-716
  • Peer Reviewed
• [Journal Article] Structural basis for the selection of glycosylated substrates by SCF^<Fbs1> ubiquitin ligase. (2007)
  • Author(s): T. Mizushida, et al.
  • Journal Title: Proc. Nat. Acad. Sci. 104 Pages: 5777-5781
  • Peer Reviewed
• [Journal Article] A neural-specific F-box protein Fbs1 functions as a chaperone suppressing glycoprotein aggregation. (2007)
  • Author(s): Y. Yoshida, et al.
  • Journal Title: J. Biol. Chem. 282 Pages: 7137-7144
  • Peer Reviewed
• [Journal Article] A neural-specific F-box protein Fbsl functions as a chaperone suppressing glycoprotein aggregation. (2007)
  • Author(s): Y.Yoshida, et. al.
  • Journal Title: J.Biol.Chem. 282 Pages: 7137-7144
  • Peer Reviewed
• [Journal Article] Structural basis for the selection of glycosylated substrates by SCF^<Fbsl> ubiquitin ligase. (2007)
  • Author(s): T.Mizushima, et. al.
  • Journal Title: Proc.Nat.Acad.Sci.U.S.A 104 Pages: 5777-5781
  • Peer Reviewed
• [Journal Article] Fbsl protects the malfoblded glycoproteins from the attack of peptide:N-glycanase. (2007)
  • Author(s): Y.Yamaguchi, et. al.
  • Journal Title: Biochem.Biophys.Res.Commun. 362 Pages: 712-716
  • Peer Reviewed
• [Journal Article] F-box proteins that contain sugar-binding domains. (2007)
  • Author(s): Y.Yoshida
  • Journal Title: Biosci.Biotechnol.Biochem. 71 Pages: 2623-2631
  • NAID: 10027520502
  • Peer Reviewed
• [Presentation] ノックアウトマウスを用いたN型糖鎖を認識するSCFFbs1ユビキチンリガーゼの解析 (2007)
  • Author(s): 中川朋子ら
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] 糖鎖認識F-boxタンパク質Fbs1の神経細胞における機能の解析 (2007)
  • Author(s): 吉田雪子、田中啓二
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] 新規F-boxタンパク質の標的分子の探索 (2007)
  • Author(s): 丸岡裕子ら
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] 糖鎖認識F-boxタンパク質Fbs 1の神経細胞における機能の解析 (2007)
  • Author(s): 吉田 雪子、田中 啓二
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] ノックアウトマウスを用いたN型糖鎖を認識するSCFFbs 1ユビキチンリガーゼの解析 (2007)
  • Author(s): 中川 朋子, ら
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] Novel functions of a neural-specific F-box protein Fbs1. (2007)
  • Author(s): Y. Yoshida & K. Tanaka
  • Organizer: EMBO Conference'Ubiquitin and Ubiquitin-like Modifiers in Cellular Regulation'
  • Year and Date: 2007-09-24
• [Presentation] Novel functions of a neural-specific F-box protein Fbsl. (2007)
  • Author(s): Y.Yoshida & K.. Tanaka
  • Organizer: EMBO Conference'Ubiquitin and Ubiquitin-like Modifiers in Cellular Regulation'
  • Place of Presentation: Riva del Garda,Italy
  • Year and Date: 2007-09-24
• [Presentation] Structural basis for the selection of glycosylated substrates by SCFFbs1 ubiquitin ligase. (2007)
  • Author(s): Y.Maruoka et al.
  • Organizer: EMBO Conference'Ubiquitin and Ubiquitin-like Modifiers in Cellular Regulation'
  • Year and Date: 2007-09-23
• [Presentation] Structural basis for the selection of glycosylated substrates by SCFFbs 1 ubiquitin ligase. (2007)
  • Author(s): Y.Maruoka, et. al.
  • Organizer: EMBO Conference'Ubiquitin and Ubiquitin-like Modifiers in Cellular Regulation'
  • Place of Presentation: Riva del Garda,Italy
  • Year and Date: 2007-09-23
• [Remarks] 研究成果データベース
  • URL: http://www.rinshoken.or.jp/database/yyosida.pdf
• [Remarks]
  • URL: http://www.rinshoken.or.jp/database/yyosida.pdf
• [Remarks]
  • URL: http://www.rinshoken.or.jp/database/yyosida.pdf