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ygienic management in the seed production system for Japanese flounder by probiotic techniques

Research Project

Project/Area Number 19580219
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionNihon University

Principal Investigator

SUGITA Haruo  Nihon University, 生物資源科学部, 教授 (50139052)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsプロバイオティクス / ヒラメ / 種苗生産 / 衛生管理 / 乳酸菌 / Lactococcus lactis / Listonella anguillarum / キチナーゼ / Vibrio / 餌料生物
Research Abstract

魚類が細菌感染したときに用いられる化学療法には多くの問題があるため、新たな対処方法が望まれている。本研究では、プロバイオティクス技術を魚類の養殖に応用し、細菌感染症の防除法を確立することを目的とした。乳酸菌やビブリオを魚類腸管から分離して、魚類に投与したところ、魚類飼育水中の病原細菌数が顕著に減少したことから、これらの細菌がヒラメなど多くの魚類のプロバイオティクス菌として有望であることが示唆された。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (45 results)

All 2009 2008 2007

All Journal Article (24 results) (of which Peer Reviewed: 16 results) Presentation (17 results) Book (4 results)

  • [Journal Article] The effect of Lactococcus lactis on the abundance of aeromonads in the rearing water of the goldfish, Carassius auratus (Linnaeus)2009

    • Author(s)
      H. Sugita, T. Fujie, T. Sagesaka and S. Itoi
    • Journal Title

      Aquacult. Res. (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 伊豆下田沖の海底から回収されたナイロンテグス表面に形成されたバイオフィルムの微生物群集2009

    • Author(s)
      杉田治男、駒田朋之、吉原喜好
    • Journal Title

      日水誌 (印刷中)

    • NAID

      10025022181

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phenotypic variation in Lactococcus lactis subsp. Lactis isolates derived from intestinal tracts of marine and freshwater fishes2009

    • Author(s)
      S. Itoi, K. Yuasa, S. Washio, T. Abe, E. Ikuno and H. Sugita
    • Journal Title

      J. Appl. Microbiol. (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biocontrol of aeromonads in freshwater culture ponds by oral administration of Lactococcus lactis2009

    • Author(s)
      H. Sugita, T. Fujie, T. Sagesaka and S. Itoi
    • Journal Title

      Proceedings of the WFC 2008 6b02

      Pages: 1-2

    • Related Report
      2008 Final Research Report
  • [Journal Article] Cetobacterium somerae, a predominant anaerobe in the intestinal tracts of freshwater fish2009

    • Author(s)
      C. Tsuchiya, T. Sakata and H. Sugita
    • Journal Title

      Proceedings of the WFC 2008 6b01

      Pages: 1-2

    • Related Report
      2008 Final Research Report
  • [Journal Article] Novel halotolerant isolates of Lactociccus lactis subsp. Lactis, isolated from intestinal tract of coastal fish2009

    • Author(s)
      S. Itoi, T. Abe, S. Washio, E. Ikuno, K. Yuasa and H. Sugita
    • Journal Title

      Proceedings of the WFC 2008 4b08

      Pages: 1-2

    • Related Report
      2008 Final Research Report
  • [Journal Article] Distribution of Vibrio parahaemolyticus and V. vulnificus in marine animals of Japanese coastal waters2009

    • Author(s)
      R. Ueda, Y. Koide, T. Sato and H. Sugita
    • Journal Title

      Proceedings of the WFC 2008 2a08

      Pages: 1-2

    • Related Report
      2008 Final Research Report
  • [Journal Article] Distribution of Vibrio parahaemolyticus and V. vulnificus in marine animals of Japanese coastal waters2009

    • Author(s)
      R. Ueda, H. Sugita, 他2名
    • Journal Title

      Proceedings of the WFC 2008 2a08

      Pages: 1-2

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Novel halotolerant isolates of Lactociccus lactis subsp. lactis, isolated from intestinal tract of coastal fish2009

    • Author(s)
      S. Itoi, H. Susita, 他4名
    • Journal Title

      Proceedings of the WFC 2008 4b08

      Pages: 1-2

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Cetobacterium somerae, a predominant anaerobe in the intestinal tracts of freshwater fish2009

    • Author(s)
      C. Tsuchiya, H. Sugita, 他1名
    • Journal Title

      Proceedings of the WFC 2008 6b01

      Pages: 1-2

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Biocontrol of aeromonads in freshwater culture ponds by oral administration of Lactococcus lactis2009

    • Author(s)
      H. Sugita, 他3名
    • Journal Title

      Proceedings of the WFC 2008 6b02

      Pages: 1-2

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Phenotypic variation in Lactococcus lact is subsp. lactis isolates derived from intestinal tracts of marine and freshwater fishes2009

    • Author(s)
      S. Itoi, H. Sugita, 他4名
    • Journal Title

      Journal of Applied Microbiology (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of halotolerant Lactococcus lactis subsp. Lactis from intestinal tract of coastal fish2008

    • Author(s)
      S. Itoi, T. Abe, S. Washio, E. Ikuno, Y. Kanomata and H. Sugita
    • Journal Title

      Int. J. Food Microbiol. 121

      Pages: 116-121

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of a fish pathogen, Listonella anguillarum in the intestinal tract of fish collected off the coast of Japan2008

    • Author(s)
      H. Sugita, H. Mizuki and S. Itoi
    • Journal Title

      Aquacult. Res. 39

      Pages: 103-105

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel ecological niche of Cetobacterium somerae, an anaerobic bacterium in the intestinal tracts of freshwater fish2008

    • Author(s)
      C. Tsuchiya, T. Sakata and H. Sugita
    • Journal Title

      Lett. Appl. Microbiol. 46

      Pages: 43-48

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel ecological niche of Cetobacterium somerae, an anaerobic bacterium in the intestinal tracts of freshwater fish2008

    • Author(s)
      C. Tsuchiya, T. Sakata and H. Sugita
    • Journal Title

      Lett. Appl. Microbiol, 46

      Pages: 43-48

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of a fish pathogen, Listonella anguillarum in the intestinal tract of fish collected off the coast of Japan2008

    • Author(s)
      H. Sugita, H. Mizuki and S. Itoi
    • Journal Title

      Aquaculture Research 39

      Pages: 103-105

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of halotolerant Lactococcus lactis subsp. lactis from intestinal tract of coastal fish2008

    • Author(s)
      S. Itoi, T. Abe, S. Washio, E. Ikuno, Y. Kanomata and H. Sugita
    • Journal Title

      Int. J. Food Microbiol. 121

      Pages: 116-121

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a novel endochitinase from a marine bacterium Vibrio proteolyticus strain No. 4422007

    • Author(s)
      S. Itoi, Y. Kanomata, Y. Koyama, K. Kadokura, S. Uchida, T. Nishio, T. Oku and H. Sugita
    • Journal Title

      Biochimica et Biophysica Acta 1774

      Pages: 1099-1107

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] An antibacterial effect of Lactococcus lactis isolated from the intestinal tract of the Amur catfish, Silurus asotus Linnaeus2007

    • Author(s)
      H. Sugita, K. Ohta, A. Kuruma and T. Sagesaka
    • Journal Title

      Aquacult. Res. 38

      Pages: 1002-1004

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of Vibrio parahaemolyticus extracellular chitinase and chitin oligosaccharide deacetylase involved in the production of heterodisaccharide from chitin2007

    • Author(s)
      K. Kadokura, A. Rokutani, M. Yamamoto, T. Ikegami, H. Sugita, S. Itoi, W. Hakamata, T. Oku and T. Nishio
    • Journal Title

      Appl. Microbiol. Biotechnol. 75

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of Vibrio parahaemolyticus extracellular chitinase and chitin oligosaccharide deacetylase involved in the production of heterodisaccharide from chitin2007

    • Author(s)
      K. Kadokura, 他8名
    • Journal Title

      Appl. Microbiol. Biotechnol. 75

      Pages: 357-365

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An antibacterial effect of Lactococcus lactis isolated from the intestinaltract of the Amur catfish, Silurus asotus Linnaeus2007

    • Author(s)
      H. Sugita, K. Ohta, A. Kuruma and T. Sagesaka
    • Journal Title

      Aquaculture Research 38

      Pages: 1002-1004

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a novel endochitinase from a marine bacterium Vibrioproteolyticus strain No. 4422007

    • Author(s)
      S. Itoi, 他7名
    • Journal Title

      Biochimica et Biophysica Acta 1774

      Pages: 1099-1107

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 異なる環境から分離した乳酸菌Lactococcus lactis subsp. Lactisの性状の比較2009

    • Author(s)
      湯浅航平、糸井史朗、阿部剛士、杉田治男
    • Organizer
      2009年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2009-03-28
    • Related Report
      2008 Final Research Report
  • [Presentation] Distribution of Vibrio parahaemolyticus and V. vulnificus in marine animals of Japanese coastal waters2008

    • Author(s)
      R. Ueda, H. Sugita, 他2名
    • Organizer
      第5回世界水産学会議
    • Place of Presentation
      横浜
    • Year and Date
      2008-10-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] Novel halotolerant isolates of Lactociccus lactis subsp. lactis, isolated from intestinal tract of coastal fish.2008

    • Author(s)
      I. Itoi, H. Sugita, 他4名
    • Organizer
      第5回世界水産学会議
    • Place of Presentation
      横浜
    • Year and Date
      2008-10-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] Cetobacterium somerae, a predominant anaerobe in the intestinal tracts of freshwater fish2008

    • Author(s)
      C. Tsuchiya, H. Sugita, 他1名
    • Organizer
      第5回世界水産学会議
    • Place of Presentation
      横浜
    • Year and Date
      2008-10-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] Biocontrol of aeromonads in freshwater culture ponds by oral administration of Lactococcus lactis2008

    • Author(s)
      H. Sugita, 他3名
    • Organizer
      第5回世界水産学会議
    • Place of Presentation
      横浜
    • Year and Date
      2008-10-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒメダカの飼育水中の細菌叢について2008

    • Author(s)
      糸井史朗、吉村 聡、幾野英里花、松本 健、杉田治男
    • Organizer
      2008年度日本水産学会春季大会
    • Place of Presentation
      東海大学
    • Year and Date
      2008-03-30
    • Related Report
      2008 Final Research Report
  • [Presentation] プロバイオティクス「魚病対策の新たなる方向性」2008

    • Author(s)
      杉田治男
    • Organizer
      平成19年度第2回日本水産学会水産増殖懇話会講演会
    • Place of Presentation
      東京大学
    • Year and Date
      2008-02-10
    • Related Report
      2008 Final Research Report
  • [Presentation] Biocontrol of aeromonads in freshwater culture ponds by oral administration of Lactococcus lactis2008

    • Author(s)
      H. Sugita, T. Fujie, T. Sagesaka and S. Itoi
    • Organizer
      5th World Fisheries Congress
    • Place of Presentation
      Pacifico Yokohama
    • Related Report
      2008 Final Research Report
  • [Presentation] Cetobacterium somerae, a predominant anaerobe in the intestinal tracts of freshwater fish2008

    • Author(s)
      C. Tsuchiya, T. Sakata and H. Sugita
    • Organizer
      5th World Fisheries Congress
    • Place of Presentation
      Pacifico Yokohama
    • Related Report
      2008 Final Research Report
  • [Presentation] Novel halotolerant isolates of Lactociccus lactis subsp. Lactis, isolated from intestinal tract of coastal fish2008

    • Author(s)
      S. Itoi, T. Abe, S. Washio, E. Ikuno, K. Yuasa and H. Sugita
    • Organizer
      5th World Fisheries Congress
    • Place of Presentation
      Pacifico Yokohama
    • Related Report
      2008 Final Research Report
  • [Presentation] Distribution of Vibrio parahaemolyticus and V. vulnificus in marine animals of Japanese coastal waters2008

    • Author(s)
      R. Ueda, Y. Koide, T. Sato and H. Sugita
    • Organizer
      5th World Fisheries Congress
    • Place of Presentation
      Pacifico Yokohama
    • Related Report
      2008 Final Research Report
  • [Presentation] Lactococcus lactis as a possible biocontrol agent against opportunistic pathogens in freshwater aquaculture2008

    • Author(s)
      H. Sugita and S. Itoi
    • Organizer
      World Aquaculture 2008
    • Place of Presentation
      Busan, Korean
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 海産魚類の腸管から分離した乳酸菌Lactococcus lactisの性状2007

    • Author(s)
      糸井史朗、阿部剛士、鷲尾明佳、幾野英里花、鹿股悠奈、杉田治男
    • Organizer
      2007年度日本水産学会秋季大会
    • Place of Presentation
      北海道大学(函館)
    • Year and Date
      2007-09-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] Vibrio proteolyticus由来キチナーゼのpH依存性に及ぼすC末端領域の影響2007

    • Author(s)
      鹿股悠奈、糸井史朗、内田晋輔、門倉一成、西尾俊幸、奥忠武、杉田治男
    • Organizer
      2007年度日本水産学会秋季大会
    • Place of Presentation
      北海道大学(函館)
    • Year and Date
      2007-09-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] Vibrio proteolyticus由来キチナーゼのPh依存性に及ぼすC末端領域の影響2007

    • Author(s)
      鹿股悠奈、糸井史朗、内田晋輔、門倉一成、西尾俊幸、奥忠武、杉田治男
    • Organizer
      2007度日本水産学会秋季大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2007-09-26
    • Related Report
      2008 Final Research Report
  • [Presentation] 海産魚類の腸管から分離した乳酸菌Lactococcus lactisの性状2007

    • Author(s)
      糸井史朗、阿部剛士、鷲尾明佳、幾野英里花、鹿股悠奈、杉田治男
    • Organizer
      2007年度日本水産学会秋季大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2007-09-26
    • Related Report
      2008 Final Research Report
  • [Presentation] 魚類腸内細菌Vibrio proteolyticus由来エンドキチナーゼの精製および活性におよぼすC末端領域の影響2007

    • Author(s)
      鹿股悠奈、糸井史朗、門倉一成、西尾俊幸、奥忠武、杉田治男
    • Organizer
      日本水産増殖学会第6回大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2007-07-18
    • Related Report
      2008 Final Research Report
  • [Book] 養殖の水と餌-陰の主役たち 杉田治男(編)2008

    • Author(s)
      杉田治男
    • Total Pages
      183
    • Publisher
      恒星社厚生閣
    • Related Report
      2008 Final Research Report
  • [Book] 養殖の餌と水-陰の主役たち2008

    • Author(s)
      杉田治男(編著)
    • Total Pages
      183
    • Publisher
      恒星社厚生閣
    • Related Report
      2007 Annual Research Report
  • [Book] 微生物の利用と制御-食の安全から環境保全まで 水産学シリーズ155 左子芳彦(編)2007

    • Author(s)
      藤井建夫、杉田治男
    • Total Pages
      143
    • Publisher
      恒星社厚生閣
    • Related Report
      2008 Final Research Report
  • [Book] 微生物の利用と制御-食の安全から環境保全まで2007

    • Author(s)
      藤井建夫・杉田治男・左子芳彦(編著)
    • Total Pages
      143
    • Publisher
      恒星社厚生閣
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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