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Discrimination method for Takifugu hybrids using AFLP and mtDNA analyses

Research Project

Project/Area Number 19580229
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionNational Fisheries University

Principal Investigator

SAKAI Harumi  National Fisheries University, 生物生産学科, 教授 (80399659)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Hiroshi  独立行政法人水産大学校, 生物生産学科, 助教 (90399650)
MATSUURA Keiichi  独立行政法人国立科学博物館, コレクションディレクター (70141984)
Research Collaborator NISHIDA Mutsumi  東京大学, 海洋研究所, 所長 (90136896)
YAMANOUE Yusuke  東京大学, 海洋研究所 (90572107)
DOI Hiroyuki  下関市立水族館, 海響館
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords分類学 / 雑種 / トラフグ属 / mtDNA / AFLP / フグ目 / ミトコンドリアDNA / 種判別マーカー / AFLP解析 / ベイズ法 / 系統分類
Research Abstract

Phylogenetic relationships of tetraodontiform fishes within teleosts, of tetraodontiform fishes, and of Takifugu species were clarified using mtDNA full sequences analysis. Hybrid analysis was also performed among 9 Takifugu species using AFLP method. All species other than between T.rubripes and T.chinensis were clearly distinguishable based on species specific AFLP markers. Their hybrids were also discriminated and 46 out of 47 hybrids were those of F1 generation. Developing of convenient primers for specific AFLP markers is needed in order to identify Takifugu hybrids as well as species.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (25 results)

All 2010 2009 2008 2007

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (15 results)

  • [Journal Article] Explosive speciation of Takifugu: Another use of fug as a model system for evolutionary biology.2009

    • Author(s)
      Yamanoue Y, Miya M, Matsuura K, Miyazawa S. Tsukamoto N, Doi H, Takahashi H, Mabuchi K, Nishida M, Sakai H
    • Journal Title

      Mol Biol Evol 26

      Pages: 623-629

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 日本沿岸から初めて記録されたタキフグTakifugu oblongus.2009

    • Author(s)
      酒井治己, 藤田勲
    • Journal Title

      水大校研報 58

      Pages: 43-44

    • NAID

      120005848099

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 日本沿岸から初めて記録されたタキフグTakifugu oblongus.2009

    • Author(s)
      酒井治己
    • Journal Title

      水産大学校研究報告 58

      Pages: 43-44

    • NAID

      120005848099

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Explosive speciation of Takifugu : Another use of fug as a model system for evolutionary biology2009

    • Author(s)
      Yamanoue Y.
    • Journal Title

      Molecular Biology and Evolution 26

      Pages: 623-629

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unique pattern of pelvic fin evolution: A case study of balistoid fishes (Pisces: Tetraodontiformes) based on whole mitochondrial genome sequences.2008

    • Author(s)
      Yamanoue Y, Miya M, Matsuura K, Sakai H, Katoh M, Nishida M
    • Journal Title

      Mol Phylogen Evol 50

      Pages: 179-189

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new perspective on phylogeny and evolution of tetraodontiform fishes (Pisces: Acanthopterygii) based on whole mitochondrial genome sequences: Basal ecological diversification?2008

    • Author(s)
      Yamanoue Y, Miya M, Matsuura K, Katoh M, Sakai H, Nishida M
    • Journal Title

      BMC Evol Biol 8

      Pages: 212-212

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new perspective on phylogeny and evolution of tetraodontiform fishes (Pisces : Acanthopterygii) based on whole mitochondrial genomu sequences : Basal ecological diversification?2008

    • Author(s)
      Yamanoue Y.
    • Journal Title

      BMC Evolutionary Biology 8

      Pages: 212-212

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unique pattern of pelvic fin evolution : A case study of balistoid fishes (Pisces : Tetraodontiformes) based on whole mitochondrial genome sequences2008

    • Author(s)
      Yamanoue Y.
    • Journal Title

      Molecular Phylogenetics and Evolution 50

      Pages: 179-189

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phylogenetic position of tetraodontiform fishes within the higher teleosts: Baysian inferences based on 44 whole mitochondrial genome sequences.2007

    • Author(s)
      Yamanoue Y, Miya M, Matsuura K, Yagishita N, Mabuchi K, Sakai H, Nishida M
    • Journal Title

      Mol Phylog Evol 45

      Pages: 89-101

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phylogenetic position of tetraodontiform fishes within the higher teleosts : Baysian inferences based on 44 whole mitochondrial2007

    • Author(s)
      Yusuke Yamanoue
    • Journal Title

      Molecular Phylogenetics and Evolution 45

      Pages: 89-101

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] ショウサイフグの初期発育とトラフグ属4種との比較.2010

    • Author(s)
      土井啓行
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      藤澤
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] ショウサイフグの初期発育とトラフグ属4種との比較.2010

    • Author(s)
      土井啓行, 園山貴之, 野村美沙紀, 石橋敏章, 今井千文, 酒井治己, 渡部終五
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      藤沢
    • Related Report
      2009 Final Research Report
  • [Presentation] AFLP法に基づくトラフグ属魚類種判別マーカーの開発.2009

    • Author(s)
      善家裕太朗, 三好博之, 高橋洋, 酒井治己
    • Organizer
      日本水産増殖学会第8回大会
    • Place of Presentation
      下関
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 周防灘におけるトラフグ放流魚の自然再生産.2009

    • Author(s)
      片町太輔, 大亀正照, 木村博, 小金隆之, 菅谷琢磨, 高橋洋, 酒井治己
    • Organizer
      平成21年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] 周防灘におけるトラフグ放流魚の自然再生産2009

    • Author(s)
      片町太輔
    • Organizer
      平成21年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] トラフグ属4種の初期発育2008

    • Author(s)
      土井啓行
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      静岡
    • Year and Date
      2008-03-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] Rapid radiation and speciation of Takifugu species in marine waters of East Asia : Another use of the fugu, Takifugu rubripes as a model.2008

    • Author(s)
      Yusuke Yamanoue
    • Organizer
      International Symposium of Systematics and Diversity of Fishes
    • Place of Presentation
      東京
    • Year and Date
      2008-03-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] フグ類の進化.水産大学校シンポジウム2008

    • Author(s)
      山野上祐介, 宮正樹, 松浦啓一, 西田睦, 酒井治己
    • Organizer
      本場・下関でフクを語る~意外に知らないフグの秘密
    • Place of Presentation
      下関
    • Related Report
      2009 Final Research Report
  • [Presentation] Rapid radiation and speciation of Takifugu species in marine waters of East Asia: Another use of the fugu2008

    • Author(s)
      Yamanoue Y, Miya M, Sakai H, Matsuura K, Miyazawa S, Miyamoto N, Takahashi H, Doi H, Nishida M
    • Organizer
      Takifugu rubripes as a model. Int Symp Syst Div Fish
    • Place of Presentation
      Tokyo
    • Related Report
      2009 Final Research Report
  • [Presentation] 淡水フグCarinotetraodon属3種の繁殖.2008

    • Author(s)
      土井啓行, 杉山由貴子, 野村美沙紀石橋敏章, 酒井治己
    • Organizer
      2008年度日本魚類学会年会
    • Place of Presentation
      松山
    • Related Report
      2009 Final Research Report
  • [Presentation] AFLP分析によって明らかになったトラフグ属魚類の自然種間交雑.2008

    • Author(s)
      善家裕太朗, 高橋洋, 酒井治己, 土井啓行, 松浦啓一, 西田睦
    • Organizer
      2008年度日本魚類学会年会
    • Place of Presentation
      松山
    • Related Report
      2009 Final Research Report
  • [Presentation] トラフグ属4種の初期発育.2008

    • Author(s)
      土井啓之, 園山貴之, 石橋敏章, 酒井治己
    • Organizer
      平成20年度日本水産学会春期大会
    • Place of Presentation
      静岡
    • Related Report
      2009 Final Research Report
  • [Presentation] AFLP分析によって明らかになったトラフグ属魚類の自然種間交雑2008

    • Author(s)
      善家裕太朗
    • Organizer
      2008年度日本魚類学会年会
    • Place of Presentation
      松山
    • Related Report
      2008 Annual Research Report
  • [Presentation] 淡水フグ Carinotetraodon 属3種の繁殖2008

    • Author(s)
      土井啓行
    • Organizer
      2008年度日本魚類学会年会
    • Place of Presentation
      松山
    • Related Report
      2008 Annual Research Report
  • [Presentation] フグ類の進化2008

    • Author(s)
      山野上祐介
    • Organizer
      水産大学校シンポジウム, 本場・下関でフクを語る〜意外に知らないフグの秘密
    • Place of Presentation
      下関
    • Related Report
      2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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