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Changes in Biochemical Properties and Food Functionalities of Myosin in Fish Myofibrils Exposed to Oxidative Stresses

Research Project

Project/Area Number 19580240
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionFukui Prefectural University

Principal Investigator

OOIZUMI Tooru  Fukui Prefectural University, 海洋生物資源学部, 教授 (20254245)

Research Collaborator XIONG Youling L.  University of Kentucky, Department of Food and Animal Sciences, Professor
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords酸化ストレス / 筋原繊維 / ミオシン / 凝集 / 架橋形成 / 過酸化脂質 / ヒドロキシラジカル / 臭素酸カリウム / 酸化 / 架橋重合 / 塩溶解性 / ジスルフィド結合 / 筋原纎維 / 分子内架橋 / 加熱変性
Research Abstract

Aggregation and cross-linking pattern of myosin in carp myofibrils exposed to hydroxyl radical, oxidized lipid or potassium bromide were investigated as a fundamental study to elucidate the effect of protein oxidation on the quality changes of fish meats during storage and processing. Aggregation and cross-linking pattern of myosin was different depending on the kind of oxidative stress. Moreover, thermal denaturation of myofibrils enhanced susceptibility of myosin to oxidative stresses.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report

Research Products

(10 results)

All 2010 2009 2008 2007

All Presentation (10 results)

  • [Presentation] Variation in cross-linking and aggregation process of myosin in carp myofibrils exposed to three different oxidizing systems.2010

    • Author(s)
      T. Ooizumi, K. Usuda, Y. Akahane, Y.L. Xiong
    • Organizer
      Institute of Food Technologists Annual Meeting
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2010-07-19
    • Related Report
      2009 Final Research Report
  • [Presentation] 各種酸化ストレスで処理したコイ筋原繊維中のミオシンのキモトリプシン消化性2010

    • Author(s)
      臼田浩紀, 大泉徹, 赤羽義章
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日本大学生物資源科学部(神奈川県藤沢市)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 各種酸化ストレスで処理したコイ筋原繊維中のミオシンのキモトリプシン消化性.2010

    • Author(s)
      臼田浩紀・大泉徹, 他
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日本大学藤沢キャンパス(神奈川県藤沢市)
    • Year and Date
      2010-03-27
    • Related Report
      2009 Final Research Report
  • [Presentation] 各種酸化ストレスによるコイ筋原繊維中のミオシンの架橋形成と凝集の進行様式の比較.2009

    • Author(s)
      臼田浩紀・大泉徹, 他
    • Organizer
      平成21年度日本水産学会秋季大会
    • Place of Presentation
      いわて県民情報交流センター・アイーナ(岩手県盛岡市)
    • Year and Date
      2009-10-02
    • Related Report
      2009 Final Research Report
  • [Presentation] 各種酸化ストレスによるコイ筋原繊維中のミオシンの架橋形成と凝集の進行様式の比較2009

    • Author(s)
      臼田浩紀, 大泉徹, 赤羽義章
    • Organizer
      平成21年度日本水産学会秋季大会
    • Place of Presentation
      いわて県民情報交流センター・アイーナ(岩手県盛岡市)
    • Year and Date
      2009-10-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] Cross-linking of myosin heavy chains in carp myofibrils as affected by the progress of protein denaturation in hydroxyl radical- oxidizing environment.2008

    • Author(s)
      T. Ooizumi, S. Ohshima T. Sekiya, Y. Akahane, Y.L. Xiong
    • Organizer
      Institute of Food Technologists Annual Meeting
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2008-06-30
    • Related Report
      2009 Final Research Report
  • [Presentation] Cross-linking of myosin heavy chains in carp myofibrils as affected by the progress of protein denaturation in hydroxyl radical-oxidizing environment2008

    • Author(s)
      T. Ooizumi, S. Ohishima, T. Sekiya, Y. Akahane
    • Organizer
      Institute of Food Technologists Annual Meeting
    • Place of Presentation
      アメリカ合衆国ニューオリンズ市
    • Year and Date
      2008-06-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒドロキシラジカルによるミオシンの架橋形成に及ぼす筋原繊維タンパク質の加熱変性の影響.2008

    • Author(s)
      大泉徹, 他
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      東海大学海洋学部(静岡県清水市)
    • Year and Date
      2008-03-28
    • Related Report
      2009 Final Research Report
  • [Presentation] ヒドロキシラジカルによるミオシンの架橋形成に及ぼす筋原繊維タンパク質の加熱変性の影響2008

    • Author(s)
      大泉 徹・大島祥子・赤羽義章
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      東海大学海洋学部
    • Year and Date
      2008-03-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] Biochemical changes in myofibrillar protein exposed to various oxidizing Systems.2007

    • Author(s)
      T. Ooizumi
    • Organizer
      98th American Oil Chemists' Society Annual Meeting and Expo
    • Place of Presentation
      Quebec City, Canada
    • Year and Date
      2007-05-14
    • Related Report
      2009 Final Research Report

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Published: 2007-03-31   Modified: 2016-04-21  

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