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Studies on development of avian embryo after ICSI

Research Project

Project/Area Number 19580325
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionNagoya University

Principal Investigator

SHIMADA Kiyoshi  Nagoya University, 生命農学研究科, 名誉教授 (40065579)

Co-Investigator(Kenkyū-buntansha) 小野 珠乙  信州大学, 農学部, 教授 (10177264)
Co-Investigator(Renkei-kenkyūsha) ONO Tamao  信州大学, 農学部, 教授 (10177264)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords細胞内精子顕微注入(ICSI) / 精子由来卵子活性化因子 / phospholipase PLCζ / cRNA / 胚盤葉 / 外来遺伝子GFP / トランスジェニックウズラ / PLCzeta / ICSI / GFP / ウズラ / 胚盤葉発生 / 顕微授精(ICSI) / phospholipase Czeta (PLCzeta) / ストロンチウム / Ca^<2+> / 発生率
Research Abstract

非ウィルス法による鳥類トランスジェニック作出は、成功率が極めて低い。よって本研究は、細胞質内精子顕微注射法(Intracytoplasmic Sperm Injection, ICSI)による遺伝子導入法を試みた。外来遺伝子としてGreen Fluorescent Protein(GFP)を単一射出精子とPLCzeta cRNAと同時に注入し、ウズラ胚にGFPを発現させるた。pCX-E GFPプラスミドとPLCzeta cRNAをウズラ未受精卵に同時に顕微注入し、24時間の体外培養(System Q1a)と48時間の追加体外培養の結果、外来遺伝子GFPを胚盤葉で発現していることが観察できた。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (19 results)

All 2009 2008 2007

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (3 results)

  • [Journal Article] Identification of differentially expressed genes involved in the regression and development of Mullerian duct of chicken2009

    • Author(s)
      Ha Y, Tsukada A, Saito N, Shimada K
    • Journal Title

      International Journal of Developmental Biology 52

      Pages: 1135-1141

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expressionof P450arom, AMH and Erα mRNA in gonads of turkey, duck and goose within one week of age2008

    • Author(s)
      Koba N, Ohfuji T, Ha Y, Mizushima S, Tsukada A, Saito N, Shimada K.
    • Journal Title

      Journal of Poultry Science 45

      Pages: 215-219

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Developmental enhancement of intracytoplasmic sperm injection(ICSI)-generated quail embryos by phospholipase Cζ2008

    • Author(s)
      Mizushima S, Takagi S, Ono T, Atsumi Y, Tsukada A, Saito N, Shimada K.
    • Journal Title

      Journal of Poultry Science 45

      Pages: 152-158

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Profiles of mRNA expression of FOXL2, P450arom, DMRT1, AMH. P450c17, SF1, Era and AR, in relation to gonadal sex differentiation in duck embryo2008

    • Author(s)
      Koba N, Ohfuji T, Ha Y, Mizushima S, Tsukada A, Saito N, Shimada K.
    • Journal Title

      Journal of Poultry Science 45

      Pages: 132-138

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of aromatase inhibitor(Fadrozole)-induced sex-reversal on gonadal differentiation and mRNA expression of P450arom, AMH and Erα in quail embryos and growth in posthaching quail2008

    • Author(s)
      Koba N, Mori M, Ha Y, Mizushima S, Tsukada A, Saito N, Shimada K
    • Journal Title

      Journal of Poultry Science 45

      Pages: 116-124

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Possible role of calcium on oocyte development after intracytoplasmic sperm injection in quail(Coturnix japonica)2008

    • Author(s)
      Mizushima S, Takagi S, Ono T, Atsumi Y, Tsukada A, Saito N, Shimada K
    • Journal Title

      Journal of Experimental Zoology 307

      Pages: 647-53

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Profiles of mRNA expression of FOXL2, P450arom, DMRT1, AMH. P450c17, SF1, ERa and AR, in relation to gonadal sex differentiation in duck embryo.2008

    • Author(s)
      Koba N, Ohfuji T, Ha Y, Mizushima S, Tsukada A, Saito N, Shimada K.
    • Journal Title

      Journal of Poultry Science 45

      Pages: 132-138

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Developmental enhancement of intracytoplasmic sperm injection (ICSI)-generated quail embryos by phospholipase Cζ2008

    • Author(s)
      Mizushima S, Takagi S, Ono T, Atsumi Y, Tsukada A, Saito N, Shimada K.
    • Journal Title

      Journal of Poultry Science 45

      Pages: 152-158

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of aromatase inhibitor (Fadrozole)-induced sex-reversal on gonadai differentiation and mRNA expression of P450axm, AMH and ERa in quail embryos and growth in posthaching quail.2008

    • Author(s)
      Koba N, Mori M, Ha Y, Mizushima S, Tsukada A, Saito N, Shimada K.
    • Journal Title

      Journal of Poultry Science 45

      Pages: 116-124

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Histocompatible chicken inbred lines : Homogeneities in the major histocompatibility complex antigens of the GSP, GSN/1, PNP/DO and BM-C inbred lines assessed by hemagglutination, mixed lymphocyte reaction and skin transplantation2007

    • Author(s)
      Valdez Jr MB, Mizutani M, Fujihara A Yazawa H, Yamagata T, Shimada K, Namikawa T
    • Journal Title

      Experimental Animals 56

      Pages: 329-338

    • NAID

      10019776577

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Z-chromosome specific primers for chicken-quail hybrid blastoderm2007

    • Author(s)
      Takagi S, Ono T, Tsukada A, Atsumi Y, Mizushima S, Saito N, Shimada K.
    • Journal Title

      Journal of Poultry Science 44

      Pages: 209-212

    • NAID

      130004446236

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing the W chromosome2007

    • Author(s)
      Takagi S, Ono T, Tsukada A, Atsumi Y, Mizushima S, Saito N, Shimada K
    • Journal Title

      Poultry Science 86

      Pages: 937-943

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fertilizing ability of chicken sperm bearing W chromosome2007

    • Author(s)
      Takagi S, Tsukada A, Saito N, Shimada K.
    • Journal Title

      Poultry Science 86

      Pages: 730-737

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Possible role of calcium on oocyte development after intracytoplasmicsperm injection in quail(Conturnix japonica)2007

    • Author(s)
      Mizushima, S., et. al.
    • Journal Title

      J Expt Zool 307A,

      Pages: 647-653

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fertilizing ability of chicken sperm bearing the W chromosome2007

    • Author(s)
      Takagi, S., et. al.
    • Journal Title

      Poultry Science 86

      Pages: 731-738

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing the W chromosome2007

    • Author(s)
      Takagi, S., et. al.
    • Journal Title

      Poultry Science 86

      Pages: 937-943

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] ウズラ卵子DNase I活性に及ぼす精子数の効果2009

    • Author(s)
      杉本宰甫、水島秀成、古寺敏子、島田清司、小野珠乙、木村一郎
    • Organizer
      日本家禽学会
    • Place of Presentation
      藤沢市
    • Year and Date
      2009-03-28
    • Related Report
      2008 Final Research Report
  • [Presentation] 顕微授精ウズラ卵cRNA注入の効果2008

    • Author(s)
      水島秀成、島田清司
    • Organizer
      日本家禽学会
    • Place of Presentation
      水戸市
    • Year and Date
      2008-03-28
    • Related Report
      2008 Final Research Report
  • [Presentation] Profiles of mRNA expression of sex-differentiation-related genes in relation to gonadal sexual differentiation in duck2007

    • Author(s)
      Naomi Koba, Shusei Mizushima, Akira Tsukada, Noboru Saito
    • Organizer
      APPC
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2007-02-06
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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