Analysis of function and regulatory mechanism of mRNA expression of bird specific transcription factor in the anterior pituitary gland
Project/Area Number |
19580330
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied animal science
|
Research Institution | Azabu University |
Principal Investigator |
KANSAKU Norio Azabu University, 獣医学部, 教授 (60333142)
|
Project Period (FY) |
2007 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 生産機能制御 / 転写制御 / Pit-1 / アイソフォーム / プロラクチン / TATAbox / レポーターアッセイ / GH3細胞 / プロモーター / イントロン / 多型 / ヒスタグ |
Research Abstract |
Intron 1 of the precocial and altricial bird of the Pit-1 gene were cloned and sequenced. Homologous position of putative translation starting site of Pit-1w/γ in the altricial species was not ATG methionin codon. Thus, it is suggested that Pit-1w/γ is not exist in the altricial species. Moreover, transcription starting region was cloned by RT-PCR. Results of this study showed that regulatory region of Pit-1w/γ is smaller than previously expected.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Changes in post-translational modifications of prolactin during development and reproductive cycles in the chicken.2009
Author(s)
Hiyama, G., Kansaku, N., Sasanami, T., Nakamura, A., Noda, K., Tsukada, A., Shimada, K., Zadworny, D.
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Journal Title
General and Comparative Endocrinology. 161
Pages: 238-245
Related Report
Peer Reviewed
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[Journal Article] Sequence of the domestic duck (Anas platyrhynchos) growth hormone-encoding gene and genetic variation in the promoter region.2008
Author(s)
Kansaku, N., Soma, A., Furukawa, S., Hiyama, G., Okabayashi, H., Guemene, D., Kuhnlein, U., Zadworny, D.
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Journal Title
Animal Science Journal. l79
Pages: 163-170
NAID
Related Report
Peer Reviewed
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[Presentation] Sequence comparison of the prolactin (PRL) promoter in BUT and Bettina turkeys2008
Author(s)
Hiyama, G., Kansaku, N., Reddy, I.J., Sotocinal, S., Guemene, D., Zadworny, D.
Organizer
9th International Symposium on Avian Endocrinology
Place of Presentation
Leuven Katholic University, Leuven, Belgium
Year and Date
2008-07-13
Related Report
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[Presentation] Comparison of prolactin in galliforms2008
Author(s)
Hiyama G, Zadworny D, Ohtsuki M, Sasanami T, Kansaku N
Organizer
9th International Symposium on Avian Endocrinology
Place of Presentation
Leuven Katholic University, Leuven, Belgium
Year and Date
2008-07-11
Related Report