Characterization of dog allergens and their application to develop a control method for dog allergy
| Project/Area Number |
19580356
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
KAMATA Yoichi National Institute of Health Sciences (20152837)
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| Project Period (FY) |
2007 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 獣医公衆衛生 / イヌアレルゲン / タンパク質構造解析 / 二次構浩 / β-sheet構造 / リポカリンファミリー / 円二色性分光法 / X線小角散乱法 / プロスタグランジンD合成酵素 / イヌ / アレルゲン / リポカリン / 脂質結合性 / 酵素阻害 / イヌアレルギー / IgE抗体 / エピトープ / 減感作 / 組換え蛋白質 / 欠失変異体 / 点変異体 |
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| Research Abstract |
Human-animal bonds become stronger. Dogs as behalf of pets are tightly associated with human living life. Due to the intimate relationship, human may be sensitized with and exhibit allergic response to dog allergens. To prevent dog allergy and assess the risk of dog allergens, characterization of dog allergens was performed. From the analysis of amino-acid sequence of Can f 1 and Can f 2 (major dog allergens), both allergens revealed the barrel structure and one disulfide bond, which were the charters of popular antigenic proteins. X-ray small angle scattering analysis and circular dichroism photometric method indicated that both allergens were globular and β-sheet-abundant proteins, and showed mono-molecular distribution in water-solutions. Also, both allergens showed the values of molecular weight in solution, which was accordance with those estimated from the amino-acid sequence. Dog albumin (Can f 3) being as one of dog allergens was also examined its allergic cross reactions. The IgE antibody in the serum of dog allergy patients bound to albumin of dog and other animal species. This fact indicates that dog allergens must be recognized as widely distributed risk substances.
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Report
(4 results)
Research Products
(10 results)
