immune response in bovine mammary gland infection

• Project/Area Number: 19580362
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Rakuno Gakuen University
• Principal Investigator: HIGUCHI Hidetoshi Rakuno Gakuen University, 獣医学部, 准教授 (00305905)
• Co-Investigator(Kenkyū-buntansha): 岩野 英知 酪農学園大学, 獣医学部, 講師 (60382488) ; 永幡 肇 酪農学園大学, 獣医学部, 教授 (10133571) ; 寺岡 宏樹 酪農学園大学, 獣医学部, 助教授 (50222146)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: ウシ / 乳房炎 / 乳腺上皮細胞 / 感染防御 / 好中球 / 活性酸素生成能 / 細胞内情報伝達系 / ウシ乳房炎 / 白血球

Research Abstract

ウシ乳房炎は生産動物の感染症において経済的損失が最も高く、畜産物の安全性を堅持する上でも大きな阻害要因となっている。乳房炎は、大腸菌や黄色ブドウ球菌など複数の病原微生物が関与する乳腺組織の感染症である。これらの原因菌は乳汁合成を阻害するとともに、乳汁に排菌された微生物は新たな感染源となる。本研究ではウシ由来乳腺上皮細胞を用い、in vitroにおける免疫学的多様性を評価し、乳腺細胞由来抗菌物質の動態および白血球機能との関連性を明らかにした。

Research Products

• [Journal Article] Characterization of bacterial population of raw milk from bovine mastitis by culture-independent PCR-DGGE method (2008)
  • Author(s): Ying Kuang, Kaori Tani, Aidan Synnott, Kazuhito Ohshima, Hidetoshi Higuchi, Hajime Nagahata, Yasunori Tanji
  • Journal Title: Biochem. Eng. J in press
  • NAID: 120006582021
  • Peer Reviewed
• [Journal Article] Functional disorder of the retina in manganese-deficient Japanese quail revealed by electroretinography using a contact lens electrode with built-in light source (2008)
  • Author(s): Kentaro Endo, Norihiko Itoh, Seiya Maehara, Aya Shinozaki, Tomohiro Imagawa, Masato Uehara, Nahoharu Mizuno, Shouji Sasaki, Takeo Hiraga T, Hiroki Teraoka
  • Journal Title: J. Vet. Med. Sci 70 Pages: 139-144
  • NAID: 110006613916
  • Peer Reviewed
• [Journal Article] Characterization of bacterial population of raw milk from bovine mastitis by culture-independent PCR-DGGE method (2008)
  • Author(s): Ying Kuang, Kaori Tani, Aidan Synnott, Kazuhito Ohshima, Hidetoshi Higuchi, Hajime Nagahata^b, Yasunori Tanji^<a*>
  • Journal Title: Biochem. Eng. J. (In press)(受理済み)
  • NAID: 120006582021
  • Peer Reviewed
• [Journal Article] Functional disorder of the retina in manganese-deficient Japanese quail revealed by electroretinography using a contact lens electrode with built-in light source. (2008)
  • Author(s): Kentaro Endo, Norihiko Itoh, Seyiya Maehara, Aya Shinozaki, Tomohiro Imagawa, Masato Uehara, Nahoharu Mizuno, Shouji Sasaki, Takeo Hiraga T, Hiroki Teraoka
  • Journal Title: J. Vet. Med. Sci. 70 Pages: 139-44
  • NAID: 110006613916
  • Peer Reviewed
• [Journal Article] Lead exposure induces pycnosis and enucleation of peripheral erythrocytes in the domestic fowl. (2008)
  • Author(s): Hiraga T, Ohyama K, Hashigaya A, Ishikawa T, Muramoto W, Kitagawa H, Mizuno N, Teraoka H.
  • Journal Title: Vet. J. 178 Pages: 109-114
  • Peer Reviewed
• [Journal Article] Complement receptor type 3 (CR3)- and Fc receptor (FcR)-mediated matrixmetaroproteinase 9 (MMP-9) secretion and their intracellular signaling of bovine neutrophils (2007)
  • Author(s): Higuchi, H., Ishizaka, M. and Nagahata, H
  • Journal Title: Vet. Res. Commun 31 Pages: 985-991
  • Peer Reviewed
• [Journal Article] Controlling highly prevalent Staphylococcus aureus mastitis from the dairy herd (2007)
  • Author(s): Nagahata, H., Ito, H., Maruta, H., Nishikawa, Y., Susukino, H., Matsuki, S., Higuchi, H., Okuhira, T. & Anri, A
  • Journal Title: J. Vet. Med. Sci 69 Pages: 893-898
  • Peer Reviewed
• [Journal Article] Antibacterial activity of bovine lactoferrin hydrolusate against mastitis pathogen its effect on superoxide production of bovine neutrophils (2007)
  • Author(s): Kawai, K., Shimazaki, K., Higuchi, H. and Nagahata, H
  • Journal Title: Zoonoses and public Health 54 Pages: 164-167
  • Peer Reviewed
• [Journal Article] Complement receptor type 3(CR3)-and Fc receptor(FcR)-mediated matrlxmetaroproteinase 9(MMP-9)secretion and their intracellular signaling of bovine neutrophils (2007)
  • Author(s): Higuchi, H., Ishiizaka, M. and Nagahata, H.
  • Journal Title: Vet.Res.Commun. 31 Pages: 985-991
  • Peer Reviewed
• [Journal Article] Controlling highly prevalent Staphylococcus aureus mastitis from the dairy herd (2007)
  • Author(s): Nagahata, H., Ito, H., Maruta, H., Nishikawa, Y., Susukino, H., Matsuki, S., Higuchi, H., Okuhira, T. & Anri, A.
  • Journal Title: J.Vet.Med.Sci. 69 Pages: 893-898
  • Peer Reviewed
• [Journal Article] Antibacterial activity of bovine lactoferrin hydrolusate against mastitis athogen its effect on superoxide production of bovine neutronhils (2007)
  • Author(s): Kawai, K., Shimazaki, K., Higuchi, H., and Nagahata, H.
  • Journal Title: Zoonoses and public Health 54 Pages: 160-164
  • Peer Reviewed
• [Presentation] 周産期乳牛における好中球α-トコフェロール濃度の評価 (2008)
  • Author(s): 北川詠子・間狩明美・樋口豪紀・永幡 肇
  • Organizer: 日本家畜衛生学会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-07
• [Presentation] L.acidophilusの乳腺内注入が乳汁の細胞学的・理化学的性状におよぼす影響 (2008)
  • Author(s): 杉山瑞恵・田中雅委・樋口豪紀・永幡肇
  • Organizer: 日本家畜衛生学会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-07
• [Presentation] マイコトキシンとウシ白血球:好中球化学発光能への影響 (2008)
  • Author(s): 難波江晶子・樋口豪紀・和田賢二・永幡肇
  • Organizer: 日本獣医学会学術集会
  • Place of Presentation: 宮崎
  • Year and Date: 2008-09-25
• [Presentation] Complement receptor type 3 (CR3)- and Fc receptor (FcR)-mediated matrix metalloproteinase 9 (MMP-9) secretion and their intracellular signaling of bovine neutrophils (2008)
  • Author(s): Higuchi, H. and Nagahata, H
  • Organizer: 世界牛病学会(WBC)
  • Place of Presentation: Hungary
  • Year and Date: 2008-07-08
• [Presentation] Complement receptor type 3 (CR3)- and Fc receptor (FcR)-mediated matrix metalloproteinase 9 (MMP-9) secretion and their intracellular signaling of bovine neutrophils (2008)
  • Author(s): Higuchi, H., Nagahata, H.
  • Organizer: 世界牛病学会(WBC)
  • Place of Presentation: Hungary (Budapest)
  • Year and Date: 2008-07-08
• [Presentation] PCR法を用いた乳房炎原因菌の迅速検出法に関する検討 (2008)
  • Author(s): 樋口豪紀・岩野英知・岩野直美・横田 博・永幡 肇
  • Organizer: 日本獣医学会学術集会
  • Place of Presentation: 神奈川
  • Year and Date: 2008-03-28
• [Presentation] 搾乳過程における生乳の衛生学的ならびに理化学的性状の変化 (2007)
  • Author(s): 久保田友香・澤山成行・樋口豪紀・永幡肇
  • Organizer: 日本家畜衛生学会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-22
• [Presentation] 黄色ブドウ球菌に対するウシ好中球の化学発光反応 (2007)
  • Author(s): 大津治子・樋口豪紀・永幡肇
  • Organizer: 日本家畜衛生学会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-22
• [Presentation] 搾乳過程における生乳の衛生学的ならびに理化学的性状の変化 (2007)
  • Author(s): 久保田 友香・澤山 成行・樋口 豪紀・永幡 肇
  • Organizer: 日本家畜衛生学会
  • Place of Presentation: 東京明治製菓本社講堂
  • Year and Date: 2007-11-22
• [Presentation] 黄色ブドウ球菌に対するウシ好中球の化学発光反応 (2007)
  • Author(s): 大津 治子・樋口 豪紀・永幡 肇
  • Organizer: 日本家畜衛生学会
  • Place of Presentation: 東京明治製菓本社講堂
  • Year and Date: 2007-11-22
• [Presentation] 大腸菌(O111:B4)によるウシ好中球活性酸素生成能の抑制について (2007)
  • Author(s): 佐藤倫奈・山本千草・樋口豪紀・永幡 肇
  • Organizer: 第144回日本獣医学会
  • Place of Presentation: 江別
  • Year and Date: 2007-09-03
• [Presentation] ビタミンE剤投与がウシ白血球のα-Tocopherol量に及ぼす影響 (2007)
  • Author(s): 伊藤えりな・坂井田佳奈・樋口豪紀・永幡 肇
  • Organizer: 第144回日本獣医学会
  • Place of Presentation: 江別
  • Year and Date: 2007-09-03
• [Presentation] 大腸菌(O111:B4)によるウシ好中球活性酸素生成能の抑制について (2007)
  • Author(s): 佐藤 倫奈・山本 千草・樋口 豪紀・永幡 肇
  • Organizer: 第144回日本獣医学会
  • Place of Presentation: 酪農学園大学
  • Year and Date: 2007-09-03
• [Presentation] ビタミンE剤投与がウシ白血球のα-Tocopherol量に及ぼす影響 (2007)
  • Author(s): 伊藤 えりな・坂井田 佳奈・樋口 豪紀・永幡 肇
  • Organizer: 第144回日本獣医学会
  • Place of Presentation: 酪農学園大学
  • Year and Date: 2007-09-03