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Development of oligoarginine-conjugated lipid vector for gene delivery

Research Project

Project/Area Number 19590046
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionHoshi University

Principal Investigator

MAITANI Yoshie  Hoshi University, 薬学部, 教授 (10231581)

Co-Investigator(Kenkyū-buntansha) KAWAKAMI Hiroko  野口研究所, 研究部, 研究員 (40320254)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords遺伝子ベクター / オリゴアルギニン / 生体膜貫通ペプチド / リポソーム / 非エンドサイトーシス / ミセル / ラクトース修飾 / 遺伝子導入 / リボソーム
Research Abstract

本研究では、遺伝子導入効率を上昇させるために、核移行能がある新規ラクトース脂質を合成し、これまでのオリゴアルギニンPEG脂質に混合したナノ粒子を調製し、グライコフェクションによる遺伝子導入ベクターの開発を目的とする。自己凝集するオリゴアルギニンPEG脂質と、ラクトースPEG脂質をそれぞれデザインし、合成した。遺伝子とラクトース修飾オリゴアルギニンミセルベクターとの複合体の構造を物理化学的手法で調べ、培養細胞における遺伝子導入効率との関係も調べた。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (22 results)

All 2009 2008 2007 Other

All Journal Article (12 results) (of which Peer Reviewed: 6 results) Presentation (8 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Decaarginine-PEG-liposome Enhanced Transfection Efficiency and Function of Arginine Length and PEG2009

    • Author(s)
      M. Furuhata, T. Izumisawa, H. Kawakami, K. Toma, Y. Hattori, Y. Maitani
    • Journal Title

      Int J Pharm 37(1-2)

      Pages: 40-46

    • Related Report
      2008 Final Research Report
  • [Journal Article] Calcium enhanced delivery of tetraarginine-PEG-lipid-coated DNA/protamine complexes2009

    • Author(s)
      T. Fujita, M. Furuhata, Y. Hattori, H. Kawakami, K. Toma, Y. Maitani
    • Journal Title

      Int J Pharm 368

      Pages: 186-192

    • Related Report
      2008 Final Research Report
  • [Journal Article] Non-ionic surfactant modified cationic liposomes mediated gene transfection in vitro and in the mouse lung2009

    • Author(s)
      W. Ding, T. Izumisawa, Y. Hattori, X. Qi, D. Kitamoto, Y. Maitani
    • Journal Title

      Biol. Pharm. Bull. 32(2)

      Pages: 311-315

    • NAID

      110007042144

    • Related Report
      2008 Final Research Report
  • [Journal Article] Surface properties of lipoplexes modified with MEL-A and Tween 802009

    • Author(s)
      W. Ding, Y. Hattori, X. Qi, D. Kitamoto, Y. Maitani
    • Journal Title

      Chem. Pharm. Bull. 57(2)

      Pages: 138-143

    • Related Report
      2008 Final Research Report
  • [Journal Article] Decaarginine-PEG-liposome Enhanced Transfection Efficiency and Function of Arginine Length and PEG2009

    • Author(s)
      M. Furuhata, T. Izumisawa, H. Kawakami, K. Toma, Y. Hattori, Y. Maitani
    • Journal Title

      Int J Pharm. 37

      Pages: 40-46

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calcium enhanced delivery of tetraarginine-PEG-lipid-coated DNA/protamine complexes2009

    • Author(s)
      T. Fujita, M. Furuhata. Y. Hattori, H. Kawakami, K. Toma, Y. Maitani
    • Journal Title

      Int J Pharm 368

      Pages: 186-192

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High gene delivery in tumor by intratumoral injection of tetraarginine-PEG lipid-coated protamine/DNA2008

    • Author(s)
      T. Fujita, M. Furuhata, Y. Hattori, H. Kawakami, K. Toma, Y. Maitani
    • Journal Title

      J. Control. Release 129(2)

      Pages: 124-127

    • Related Report
      2008 Final Research Report
  • [Journal Article] Novel oligoarginine-linked PEG-lipid micelles for gene delivery2008

    • Author(s)
      M. Furuhata, Y. Hattori and Y. Maitani
    • Journal Title

      Proc. Hoshi Univ. 50

      Pages: 21-32

    • NAID

      40016648030

    • Related Report
      2008 Final Research Report
  • [Journal Article] High gene delivery in tumor by intratumoral injection of tetraarginine-PEG lipid-coated protamine/DNA2008

    • Author(s)
      T. Fujita, M. Furuhata, Y. Hattori, H. Kawakami, K. Toma, Y. Maitani
    • Journal Title

      J. Control. Release 129

      Pages: 124-127

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Highly efficient cationic hydroxyethylated cholesterol-based nanoparticle-mediated gene transfer in vivo and in vitro in prostate carcinoma PC-3 cells2007

    • Author(s)
      Y.Hattori, W.Ding, Y.Maitani
    • Journal Title

      J Control Release 120

      Pages: 122-130

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-circulating liposome-encapsulated gancicloVir enhallces the efficacy of HSV-TK suicide gene therapy2007

    • Author(s)
      E.Kajiwara, K.Kawano, Y.Hattori, M.Fukushma, K.Hayashi, Y.Maitani
    • Journal Title

      J Control Release 120

      Pages: 104-110

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cationic liposome(DC-Chol/DOPE=1:2)and a modified ethanol injection method to prepare liposomes,increased gene expression2007

    • Author(s)
      Y.Maitani, S.Igarashi, M.Sato, Y.Hattori
    • Journal Title

      Int.J.Pharm 342

      Pages: 33-39

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] オリゴアルギニン脂質/DNA複合体の細胞内動態2008

    • Author(s)
      小林昇平、古幡昌彦、R. Danev、永山國昭, 戸澗一孔、服部喜之、米谷芳枝、原口徳子
    • Organizer
      遺伝子・デリバリー研究会第8回シンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2008-05-09
    • Related Report
      2008 Final Research Report
  • [Presentation] オリゴアルギニンPEG修飾リポソームによる細胞内取り込み経路および遺伝子発現2008

    • Author(s)
      古幡昌彦、川上宏子、戸澗一孔、米谷芳枝
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] MEL-A modified MHAPC-liposomes for gene delivery in vitro and in the lung2008

    • Author(s)
      丁武孝、服部喜之、米谷芳枝
    • Organizer
      第10回応用薬理シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] MEL-A and Tween 80 modified cationic liposomes for gene transfection in vitro and in the lung2008

    • Author(s)
      丁武孝、泉澤友宏、服部喜之、米谷芳枝
    • Organizer
      11th LIPOSOME RESEARCH DAYS CONFERENCE
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] Liposomal Drug Delivery : An Update2008

    • Author(s)
      Y. Maitani
    • Organizer
      22nd Federation of Asian Pharmaceutical Association Congress
    • Place of Presentation
      シンガポール
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] オリゴアルギニンミセル/DNA複合体の構造と遺伝子導入効率2007

    • Author(s)
      古幡昌彦、川上宏子、戸澗一孔、服部喜之、米谷芳枝
    • Organizer
      日本薬剤学会第22年会
    • Place of Presentation
      埼玉
    • Related Report
      2008 Final Research Report
  • [Presentation] オリゴアルギニン脂質ベクターによる遺伝子の細胞内移行促進2007

    • Author(s)
      古幡昌彦
    • Organizer
      日本薬剤学会第22年会
    • Place of Presentation
      埼玉
    • Related Report
      2008 Final Research Report
  • [Presentation] オリゴアルギニンミセル/DNA複合体の構造と遺伝子導入効率2007

    • Author(s)
      古幡 昌彦、川上 宏子、服部 喜之、戸澗 一孔、米谷 芳枝
    • Organizer
      日本薬剤学会第22年会
    • Place of Presentation
      大宮
    • Related Report
      2007 Annual Research Report
  • [Book] Tumor-targeting non-Viral gene therapy for the treatment of oral cancer, Cancer of the Mouth Research Trends2007

    • Author(s)
      Y.Hattori, Y.Maitani
    • Publisher
      Nova Science Pubhshers,Inc
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://polaris.hoshi.ac.jp/kyoshitsu/souzai/index1.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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